################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII ClaI DraI DraIII EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HindIII KasI KpnI KspI MamI MfeI MflI MluI MunI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tsp45I Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccI 100 GTCTAC GT^[AC][GT]AC AccII 105 CGCG CG^CG AccIII 306 TCCGGA T^CCGGA AcyI 616 GACGCC G[GA]^CG[CT]C Aor51HI 62 AGCGCT AGC^GCT AvaI 276 CTCGGG C^[CT]CG[GA]G BalI 927 TGGCCA TGG^CCA BcgI 135 CGACGTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 594 CCGGG CC^[GC]GG BfaI 871 CTAG C^TAG BsaHI 616 GACGCC G[GA]^CG[CT]C BsaI 572 GAGACC GGTCTC(1/5) BsaWI 306 TCCGGA [AT]^CCGG[AT] BseAI 306 TCCGGA T^CCGGA BseRI 502 GAGGAG GAGGAG(10/8) BsgI 540 CTGCAC GTGCAG(16/14) BsmAI 572 GAGAC GTCTC(1/5) BsmFI 694 GGGAC GGGAC(10/14) BsoBI 276 CTCGGG C^[CT]CG[GA]G BspEI 306 TCCGGA T^CCGGA BspHI 605 TCATGA T^CATGA BsrFI 145 GCCGGC [GA]^CCGG[CT] BstUI 105 CGCG CG^CG Cac8I 145 GCCGGC GC[ACGT]^[ACGT]GC Cfr10I 145 GCCGGC [GA]^CCGG[CT] CpoI 107 CGGACCG CG^G[AT]CCG DpnII 603 GATC ^GATC DraII 694 GGGACCC [GA]G^G[ACGT]CC[CT] DsaI 930 CCATGG C^C[GA][CT]GG EarI 289 CTCTTC CTCTTC(1/4) Eco47III 62 AGCGCT AGC^GCT EcoO109I 694 GGGACCC [GA]G^G[ACGT]CC[CT] HgaI 616 GACGC GACGC(5/10) Hin1I 616 GACGCC G[GA]^CG[CT]C HpaI 453 GTTAAC GTT^AAC Ksp632I 289 CTCTTC CTCTTC(1/4) MaeI 871 CTAG C^TAG MaeIII 706 GTTAC ^GT[ACGT]AC MboI 603 GATC ^GATC MluNI 927 TGGCCA TGG^CCA MroI 306 TCCGGA T^CCGGA MscI 927 TGGCCA TGG^CCA MseI 454 TTAA T^TAA MspA1I 481 CAGCGG C[AC]G^C[GT]G MvnI 105 CGCG CG^CG NaeI 145 GCCGGC GCC^GGC NciI 594 CCGGG CC^[GC]GG NcoI 930 CCATGG C^CATGG NdeII 603 GATC ^GATC NgoMIV 145 GCCGGC G^CCGGC PflMI 950 CCATCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 694 GGGACCC [GA]G^G[AT]CC[CT] RcaI 605 TCATGA T^CATGA RsrII 107 CGGACCG CG^G[AT]CCG Sau3AI 603 GATC ^GATC Tru9I 454 TTAA T^TAA Van91I 950 CCATCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 128 CCACCACCGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AfaI 681 GTAC GT^AC 853 GTAC AlwNI 82 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG 436 CAGTGGCTG BanII 799 GAGCCC G[GA]GC[CT]^C 943 GGGCTC BglI 929 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 967 GCCCTGGAGGC BsmI 813 GAATGC GAATGC(1/-1) 1022 GAATGC DrdI 91 GACATAAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 526 GACGTGATTGTC EaeI 847 CGGCCA [CT]^GGCC[GA] 927 TGGCCA EcoT14I 622 CCTTGG C^C[AT][AT]GG 930 CCATGG HaeII 62 AGCGCT [GA]GCGC^[CT] 561 GGCGCT HincII 453 GTTAAC GT[CT]^[GA]AC 535 GTCAAC HindII 453 GTTAAC GT[CT]^[GA]AC 535 GTCAAC MaeII 137 ACGT A^CGT 527 ACGT MwoI 929 GCCATGGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 967 GCCCTGGAGGC PleI 26 GAGTC GAGTC(4/5) 493 GAGTC RsaI 681 GTAC GT^AC 853 GTAC StyI 622 CCTTGG C^C[AT][AT]GG 930 CCATGG TfiI 864 GATTC G^A[AT]TC 995 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AluI Asp700I AvaII BbvI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI BstXI CfoI Cfr13I DdeI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MboII MnlI MslI MspI MvaI NlaIII NlaIV PstI Sau96I ScrFI SfaNI SfcI TseI Tsp509I TspRI XmnI