Restriction map for
pFN21AE4336
(Circular) (Six-base) MAPSORT of: pFN21AE4336.seq Check: 280 from: 1 to: 5746
pFN21AE4336 5746 bp
With 182 enzymes: SgfI *
March 28, 2012 13:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3556 278
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2106 3089 1937
Size: 169 983 4594
Fragments arranged by size:
4594 983 169
Acc65I G'GTAC_C
Cuts at: 3067 3067
Size: 5746
AclI AA'CG_TT
Cuts at: 1563 3873 4246 1563
Size: 2310 373 3063
Fragments arranged by size:
3063 2310 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2834 3809 5193 5461 1632
Size: 1202 975 1384 268 1917
Fragments arranged by size:
1917 1384 1202 975 268
AfeI AGC'GCT
Cuts at: 2069 2069
Size: 5746
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5549
AflIII A'CryG_T
Cuts at: 1203 4645 5622 1203
Size: 3442 977 1327
Fragments arranged by size:
3442 1327 977
AgeI A'CCGG_T
Cuts at: 4577 4577
Size: 5746
AhdI GACnn_n'nnGTC
Cuts at: 4475 4475
Size: 5746
AleI CACnn'nnGTG
Cuts at: 1166 2141 1166
Size: 975 4771
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5746
AlwNI CAG_nnn'CTG
Cuts at: 2092 2446 3489 5061 2092
Size: 354 1043 1572 2777
Fragments arranged by size:
2777 1572 1043 354
ApaLI G'TGCA_C
Cuts at: 3803 4959 3803
Size: 1156 4590
ApoI r'AATT_y
Cuts at: 1493 3055 3281 3433 5542 1493
Size: 1562 226 152 2109 1697
Fragments arranged by size:
2109 1697 1562 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4300 160
Size: 872 3268 1606
Fragments arranged by size:
3268 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
AvaI C'yCGr_G
Cuts at: 1870 1943 2281 3071 1870
Size: 73 338 790 4545
Fragments arranged by size:
4545 790 338 73
BamHI G'GATC_C
Cuts at: 3076 3443 3076
Size: 367 5379
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3067 4522 618
Size: 325 217 627 1280 1455 1842
Fragments arranged by size:
1842 1455 1280 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2808 2952 3065 1391
Size: 1417 144 113 4072
Fragments arranged by size:
4072 1417 144 113
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4775
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2129 2163 3937 3971 1903
Size: 34 50 34 108 34 1774 34 3678
Fragments arranged by size:
3678 1774 108 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3638 4848 3638
Size: 1210 4536
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5746
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2940 2978 4357
Size: 107 122 71 631 605 1268 38 1379
Cuts at: 4357 136
Size: 1525
Fragments arranged by size:
1525 1379 1268 631 605 122 107 71
38
BglII A'GATC_T
Cuts at: 5741 5741
Size: 5746
BlpI GC'TnA_GC
Cuts at: 3163 3163
Size: 5746
Bme1580I G_kGCm'C
Cuts at: 1790 2974 3807 4963 1790
Size: 1184 833 1156 2573
Fragments arranged by size:
2573 1184 1156 833
BmgBI CAC'GTC
Cuts at: 1318 2143 2533 1318
Size: 825 390 4531
Fragments arranged by size:
4531 825 390
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2559 2971 3477
Size: 416 53 83 399 24 1142 412 506
Cuts at: 3477 4435 442
Size: 958 1753
Fragments arranged by size:
1753 1142 958 506 416 412 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2224 2441 2764 2822 2996 4406 1239
Size: 985 217 323 58 174 1410 2579
Fragments arranged by size:
2579 1410 985 323 217 174 58
Bpu10I CC'TnA_GC
Cuts at: 2231 2231
Size: 5746
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3871 4736 5034 3871
Size: 865 298 4583
Fragments arranged by size:
4583 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2571 4409 882
Size: 617 1072 1838 2219
Fragments arranged by size:
2219 1838 1072 617
BsaAI yAC'GTr
Cuts at: 493 1204 3537 493
Size: 711 2333 2702
Fragments arranged by size:
2702 2333 711
BsaBI GATnn'nnATC
Cuts at: 5740 5740
Size: 5746
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2622 3553 3935 4570
Size: 53 83 186 936 1089 931 382 635
Cuts at: 4570 275
Size: 1451
Fragments arranged by size:
1451 1089 936 931 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2311 4179 4577 4851 4998 5409 2311
Size: 1868 398 274 147 411 2648
Fragments arranged by size:
2648 1868 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2016 2046 1178
Size: 30 165 30 613 30 4878
Fragments arranged by size:
4878 613 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2522 1186
Size: 1336 4410
BseYI C'CCAG_C
Cuts at: 2561 4949 5445 2561
Size: 2388 496 2862
Fragments arranged by size:
2862 2388 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2530 1163
Size: 573 794 4379
Fragments arranged by size:
4379 794 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3196 3957 4985 1540
Size: 275 186 1195 761 1028 2301
Fragments arranged by size:
2301 1195 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3807 3892 4963 3807
Size: 85 1071 4590
Fragments arranged by size:
4590 1071 85
BsmI GAATG_Cn'
Cuts at: 2824 3033 3257 3350 2824
Size: 209 224 93 5220
Fragments arranged by size:
5220 224 209 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2808 2952 2974 3065 3807 3892 4963
Size: 399 1018 144 22 91 742 85 1071
Cuts at: 4963 1391
Size: 2174
Fragments arranged by size:
2174 1071 1018 742 399 144 91 85
22
BspEI T'CCGG_A
Cuts at: 2311 5409 2311
Size: 3098 2648
BspHI T'CATG_A
Cuts at: 2610 3635 5365 2610
Size: 1025 1730 2991
Fragments arranged by size:
2991 1730 1025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3101 844
Size: 2257 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3633 1394
Size: 429 1810 3507
Fragments arranged by size:
3507 1810 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4241 4415 65
Size: 1183 2993 174 1396
Fragments arranged by size:
2993 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2150 4390 4577 1700
Size: 110 340 2240 187 2869
Fragments arranged by size:
2869 2240 340 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5746
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5746
BssSI C'ACGA_G
Cuts at: 3806 4818 3806
Size: 1012 4734
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5746
BstBI TT'CG_AA
Cuts at: 4550 4550
Size: 5746
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5746
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2143 2368 2930 2011
Size: 132 225 562 4827
Fragments arranged by size:
4827 562 225 132
BstYI r'GATC_y
Cuts at: 1972 3076 3443 3828 3845 5297 5383 5395 5741
Size: 1104 367 385 17 1452 86 12 346
Cuts at: 5741 1972
Size: 1977
Fragments arranged by size:
1977 1452 1104 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2935 513
Size: 552 1870 3324
Fragments arranged by size:
3324 1870 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5007
Fragments arranged by size:
5007 683 56
BtsI GCAGTG_nn'
Cuts at: 3262 4056 4076 3262
Size: 794 20 4932
Fragments arranged by size:
4932 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5746
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5711
DraI TTT'AAA
Cuts at: 3050 3050
Size: 5746
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2102 2537 4753 809
Size: 507 786 435 2216 1802
Fragments arranged by size:
2216 1802 786 507 435
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2852 2932 3193
Size: 54 1425 50 275 18 1022 80 261
Cuts at: 3193 4082 5711 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1022 889 275 261 80 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3193 1537
Size: 275 1381 4090
Fragments arranged by size:
4090 1381 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2300 3676 1353
Size: 947 1376 3423
Fragments arranged by size:
3423 1376 947
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2213 4333 4707 4853 387
Size: 1064 138 624 2120 374 146 1280
Fragments arranged by size:
2120 1280 1064 624 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2224 2441 2764 2822 2834 2996 3809
Size: 393 592 217 323 58 12 162 813
Cuts at: 3809 4406 5193 5461 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 813 787 597 592 393 323 268
217 162 58 12
EcoO109I rG'GnC_Cy
Cuts at: 2700 2700
Size: 5746
EcoRI G'AATT_C
Cuts at: 3055 3433 3055
Size: 378 5368
FspI TGC'GCA
Cuts at: 4252 4252
Size: 5746
HaeII r_GCGC'y
Cuts at: 1678 2071 2570 4893 1678
Size: 393 499 2323 2531
Fragments arranged by size:
2531 2323 499 393
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2016 2048 2698 2730 4387
Size: 32 135 32 444 32 650 32 1657
Cuts at: 4387 4419 4461 4493 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1657 650 444 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2460 2542 3090 3336 669
Size: 988 281 522 82 548 246 3079
Fragments arranged by size:
3079 988 548 522 281 246 82
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5746
HpaI GTT'AAC
Cuts at: 2460 3336 2460
Size: 876 4870
KpnI G_GTAC'C
Cuts at: 3071 3071
Size: 5746
MfeI C'AATT_G
Cuts at: 3345 3345
Size: 5746
MluI A'CGCG_T
Cuts at: 5622 5622
Size: 5746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4860 5044 1305
Size: 3555 184 2007
Fragments arranged by size:
3555 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2934 10
Size: 54 1425 343 1102 2822
Fragments arranged by size:
2822 1425 1102 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2141 2615 2928 3704 4063 4222
Size: 648 843 132 474 313 776 359 159
Cuts at: 4222 518
Size: 2042
Fragments arranged by size:
2042 843 776 648 474 359 313 159
132
MspA1I CmG'CkG
Cuts at: 1606 2488 3162 3839 4987 5232 1606
Size: 882 674 677 1148 245 2120
Fragments arranged by size:
2120 1148 882 677 674 245
NaeI GCC'GGC
Cuts at: 2152 2152
Size: 5746
NcoI C'CATG_G
Cuts at: 513 1065 2935 513
Size: 552 1870 3324
Fragments arranged by size:
3324 1870 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5746
NgoMIV G'CCGG_C
Cuts at: 2150 2150
Size: 5746
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5746
NotI GC'GGCC_GC
Cuts at: 3193 3193
Size: 5746
NspI r_CATG'y
Cuts at: 1132 1229 3104 4649 1132
Size: 97 1875 1545 2229
Fragments arranged by size:
2229 1875 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2696 1348
Size: 1348 4398
PciI A'CATG_T
Cuts at: 4645 4645
Size: 5746
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2961 1108
Size: 1853 3893
PfoI T'CCnGG_A
Cuts at: 2321 2763 3003 2321
Size: 442 240 5064
Fragments arranged by size:
5064 442 240
PmeI GTTT'AAAC
Cuts at: 3050 3050
Size: 5746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3815 3847 5328 5360 3815
Size: 32 1481 32 4201
Fragments arranged by size:
4201 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2700 2700
Size: 5746
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5746
PsiI TTA'TAA
Cuts at: 3316 3316
Size: 5746
PstI C_TGCA'G
Cuts at: 830 2088 2592 2798 3098 830
Size: 1258 504 206 300 3478
Fragments arranged by size:
3478 1258 504 300 206
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5746
RsrII CG'GwC_CG
Cuts at: 2113 2113
Size: 5746
SalI G'TCGA_C
Cuts at: 1936 3088 1936
Size: 1152 4594
SanDI GG'GwC_CC
Cuts at: 2700 2700
Size: 5746
SbfI CC_TGCA'GG
Cuts at: 3098 3098
Size: 5746
ScaI AGT'ACT
Cuts at: 3994 5706 3994
Size: 1712 4034
SfcI C'TryA_G
Cuts at: 826 1045 2084 2438 2588 2794 3094 4229 4910
Size: 219 1039 354 150 206 300 1135 681
Cuts at: 4910 5101 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1039 681 354 300 219 206
191 150
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5746
SmaI CCC'GGG
Cuts at: 1872 3073 1872
Size: 1201 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3850 4751 5013 820
Size: 197 926 1907 901 262 1553
Fragments arranged by size:
1907 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3537 493
Size: 3044 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5746
SphI G_CATG'C
Cuts at: 1132 3104 1132
Size: 1972 3774
SspI AAT'ATT
Cuts at: 5 52 3670 5
Size: 47 3618 2081
Fragments arranged by size:
3618 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5746
StyI C'CwwG_G
Cuts at: 513 1065 2627 2935 3185 513
Size: 552 1562 308 250 3074
Fragments arranged by size:
3074 1562 552 308 250
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2666 3773 3790 3943 4128 4573 325
Size: 1255 1086 1107 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1107 1086 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3992 5704 96
Size: 274 80 33 51 1443 2015 1712 138
Fragments arranged by size:
2015 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5706 5738 5706
Size: 32 5714
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5746
XbaI T'CTAG_A
Cuts at: 3082 3082
Size: 5746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2140 2011
Size: 129 5617
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5746
XmaI C'CCGG_G
Cuts at: 1870 3071 1870
Size: 1201 4545
XmnI GAAnn'nnTTC
Cuts at: 2307 2480 2639 2889 3875 2307
Size: 173 159 250 986 4178
Fragments arranged by size:
4178 986 250 173 159
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3554 276
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI RsrII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspOMI PspXI PsrI PvuII SacI
SacII SapI SexAI SfiI SfoI SrfI SwaI