################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflIII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvrII BamHI BbeI BciVI BclI BglI BlnI BsaHI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DrdI DsaI Eam1105I EarI Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HgaI Hin1I HincII HindII HpaI KasI KpnI Ksp632I KspI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tsp509I Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 604 CGCG CG^CG AflII 1315 CTTAAG C^TTAAG Aor51HI 431 AGCGCT AGC^GCT AvaI 403 CTCGAG C^[CT]CG[GA]G AviII 1262 TGCGCA TGC^GCA BbrPI 964 CACGTG CAC^GTG BbsI 340 GAAGAC GAAGAC(2/6) BfaI 796 CTAG C^TAG BfrI 1315 CTTAAG C^TTAAG BpuAI 340 GAAGAC GAAGAC(2/6) BsaAI 964 CACGTG [CT]AC^GT[GA] BsaBI 1206 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 841 GAGACC GGTCTC(1/5) BsgI 556 CTGCAC GTGCAG(16/14) BsiEI 418 CGGCCG CG[GA][CT]^CG BsoBI 403 CTCGAG C^[CT]CG[GA]G BsrBI 1279 GAGCGG CCGCTC(-3/-3) BsrDI 780 CATTGC GCAATG(2/0) BstAPI 477 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 604 CGCG CG^CG BstXI 935 CCAGGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 961 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 418 CGGCCG C^GGCCG EclXI 418 CGGCCG C^GGCCG Eco47III 431 AGCGCT AGC^GCT Eco52I 418 CGGCCG C^GGCCG EcoT14I 1094 CCTTGG C^C[AT][AT]GG FspI 1262 TGCGCA TGC^GCA HindIII 5 AAGCTT A^AGCTT HinfI 376 GAGTC G^A[ACGT]TC MaeI 796 CTAG C^TAG MaeII 965 ACGT A^CGT MamI 1206 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 1316 TTAA T^TAA MvnI 604 CGCG CG^CG PaeR7I 403 CTCGAG C^TCGAG PleI 376 GAGTC GAGTC(4/5) PmaCI 964 CACGTG CAC^GTG PmlI 964 CACGTG CAC^GTG PpuMI 911 AGGACCT [GA]G^G[AT]CC[CT] SacI 469 GAGCTC GAGCT^C SexAI 47 ACCAGGT A^CC[AT]GGT StuI 257 AGGCCT AGG^CCT StyI 1094 CCTTGG C^C[AT][AT]GG Tru9I 1316 TTAA T^TAA XcmI 779 CCATTGCCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 403 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### Asp700I 21 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1230 GAACCCCTTC BanI 321 GGTGCC G^G[CT][GA]CC 821 GGTGCC BanII 282 GAGCCC G[GA]GC[CT]^C 469 GAGCTC BcgI 606 CGAGCACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1365 CGAGGGCTGTGC BglII 59 AGATCT A^GATCT 1124 AGATCT BsaWI 11 ACCGGA [AT]^CCGG[AT] 644 ACCGGA BsmAI 841 GAGAC GTCTC(1/5) 1019 GTCTC BsmFI 185 GGGAC GGGAC(10/14) 1069 GGGAC BsrFI 154 GCCGGT [GA]^CCGG[CT] 509 ACCGGC BsrI 992 ACTGG ACTGG(1/-1) 1158 CCAGT BssSI 515 CTCGTG CACGAG(-5/-1) 1000 CTCGTG Cfr10I 154 GCCGGT [GA]^CCGG[CT] 509 ACCGGC EcoNI 795 CCTAGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1059 CCTGTACGAGG HaeII 431 AGCGCT [GA]GCGC^[CT] 811 AGCGCC MboII 340 GAAGA GAAGA(8/7) 1172 TCTTC MslI 236 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 538 CACCTCCGTG PflMI 366 CCAGGCGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 390 CCACCTGCTGG PstI 169 CTGCAG CTGCA^G 238 CTGCAG PvuII 1249 CAGCTG CAG^CTG 1258 CAGCTG TaqI 275 TCGA T^CGA 404 TCGA TspRI 236 CACTG CA[GC]TG[ACGT][ACGT]^ 241 CAGTG TthHB8I 275 TCGA T^CGA 404 TCGA Van91I 366 CCAGGCGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 390 CCACCTGCTGG XmnI 21 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1230 GAACCCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaII BalI BbvCI BbvI BcnI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsiHKAI BsiYI BslI Bsp1286I BspMI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeIII MboI MflI MluNI MnlI MscI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TseI Tsp45I XhoII