(Circular) (Six-base) MAPSORT of: pFN21AE3916.seq Check: 4083 from: 1 to: 6109 pFN21AE3916 6109 bp With 182 enzymes: SgfI * March 28, 2012 11:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2406 2568 2687 2406 Size: 162 119 5828 Fragments arranged by size: 5828 162 119 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3919 278 Size: 53 83 186 936 2383 2468 Fragments arranged by size: 2468 2383 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3452 1937 Size: 1515 4594 Acc65I G'GTAC_C Cuts at: 3430 3430 Size: 6109 AclI AA'CG_TT Cuts at: 1563 4236 4609 1563 Size: 2673 373 3063 Fragments arranged by size: 3063 2673 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2372 4172 5556 5824 1632 Size: 740 1800 1384 268 1917 Fragments arranged by size: 1917 1800 1384 740 268 AfeI AGC'GCT Cuts at: 2438 2438 Size: 6109 AflII C'TTAA_G Cuts at: 820 1017 3320 820 Size: 197 2303 3609 Fragments arranged by size: 3609 2303 197 AflIII A'CryG_T Cuts at: 1203 5008 5985 1203 Size: 3805 977 1327 Fragments arranged by size: 3805 1327 977 AgeI A'CCGG_T Cuts at: 4940 4940 Size: 6109 AhdI GACnn_n'nnGTC Cuts at: 4838 4838 Size: 6109 AleI CACnn'nnGTG Cuts at: 1166 2245 2547 1166 Size: 1079 302 4728 Fragments arranged by size: 4728 1079 302 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2637 1184 Size: 1453 4656 AlwNI CAG_nnn'CTG Cuts at: 2133 2488 2560 2587 2767 2920 3169 3852 5424 Size: 355 72 27 180 153 249 683 1572 Cuts at: 5424 2133 Size: 2818 Fragments arranged by size: 2818 1572 683 355 249 180 153 72 27 ApaLI G'TGCA_C Cuts at: 4166 5322 4166 Size: 1156 4953 ApoI r'AATT_y Cuts at: 1493 3418 3644 3796 5905 1493 Size: 1925 226 152 2109 1697 Fragments arranged by size: 2109 1925 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4663 160 Size: 872 3631 1606 Fragments arranged by size: 3631 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6109 AvaI C'yCGr_G Cuts at: 1870 1943 2408 3434 1870 Size: 73 465 1026 4545 Fragments arranged by size: 4545 1026 465 73 BamHI G'GATC_C Cuts at: 3439 3806 3439 Size: 367 5742 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2326 2826 3430 4885 618 Size: 325 217 627 539 500 604 1455 1842 Fragments arranged by size: 1842 1455 627 604 539 500 325 217 BanII G_rGCy'C Cuts at: 1391 2291 2478 3428 1391 Size: 900 187 950 4072 Fragments arranged by size: 4072 950 900 187 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2352 928 Size: 971 453 4685 Fragments arranged by size: 4685 971 453 BbvCI CC'TCA_GC Cuts at: 2219 2429 2467 2219 Size: 210 38 5861 Fragments arranged by size: 5861 210 38 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2600 2634 3359 3393 4300 4334 1903 Size: 34 663 34 725 34 907 34 3678 Fragments arranged by size: 3678 907 725 663 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4001 5211 4001 Size: 1210 4899 BclI T'GATC_A Cuts at: 1548 1548 Size: 6109 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4720 136 Size: 107 122 71 631 605 3048 1525 Fragments arranged by size: 3048 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2064 3129 6104 2064 Size: 1065 2975 2069 Fragments arranged by size: 2975 2069 1065 BlpI GC'TnA_GC Cuts at: 2285 2591 3297 3526 2285 Size: 306 706 229 4868 Fragments arranged by size: 4868 706 306 229 Bme1580I G_kGCm'C Cuts at: 1790 2331 2466 4170 5326 1790 Size: 541 135 1704 1156 2573 Fragments arranged by size: 2573 1704 1156 541 135 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6109 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3840 4798 442 Size: 416 53 83 399 24 2423 958 1753 Fragments arranged by size: 2423 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6109 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2089 2548 2553 2674 2897 3311 3332 3356 Size: 850 459 5 121 223 414 21 24 Cuts at: 3356 4769 1239 Size: 1413 2579 Fragments arranged by size: 2579 1413 850 459 414 223 121 24 21 5 Bpu10I CC'TnA_GC Cuts at: 2219 2429 2467 2219 Size: 210 38 5861 Fragments arranged by size: 5861 210 38 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2462 3154 4234 5099 5397 2462 Size: 692 1080 865 298 3174 Fragments arranged by size: 3174 1080 865 692 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2840 4772 882 Size: 617 1341 1932 2219 Fragments arranged by size: 2219 1932 1341 617 BsaAI yAC'GTr Cuts at: 493 1204 2971 3900 493 Size: 711 1767 929 2702 Fragments arranged by size: 2702 1767 929 711 BsaBI GATnn'nnATC Cuts at: 3215 6103 3215 Size: 2888 3221 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3916 4298 4933 275 Size: 53 83 186 936 2383 382 635 1451 Fragments arranged by size: 2383 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2016 2649 4542 4940 5214 5361 5772 2016 Size: 633 1893 398 274 147 411 2353 Fragments arranged by size: 2353 1893 633 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5884 Fragments arranged by size: 5884 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2819 2822 2825 1186 Size: 1633 3 3 4470 Fragments arranged by size: 4470 1633 3 3 BseYI C'CCAG_C Cuts at: 2255 2401 2439 5312 5808 2255 Size: 146 38 2873 496 2556 Fragments arranged by size: 2873 2556 496 146 38 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2546 1163 Size: 573 810 4726 Fragments arranged by size: 4726 810 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2426 3559 4320 5348 1540 Size: 275 186 425 1133 761 1028 2301 Fragments arranged by size: 2301 1133 1028 761 425 275 186 BsiHKAI G_wGCw'C Cuts at: 2210 2478 2616 2913 4170 4255 5326 2210 Size: 268 138 297 1257 85 1071 2993 Fragments arranged by size: 2993 1257 1071 297 268 138 85 BsmI GAATG_Cn' Cuts at: 3620 3713 3620 Size: 93 6016 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2210 2291 2331 2466 2478 2616 2913 Size: 399 420 81 40 135 12 138 297 Cuts at: 2913 3428 4170 4255 5326 1391 Size: 515 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 515 420 399 297 138 135 85 81 40 12 BspEI T'CCGG_A Cuts at: 5772 5772 Size: 6109 BspHI T'CATG_A Cuts at: 3998 5728 3998 Size: 1730 4379 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2406 2568 2687 2928 3177 3464 844 Size: 1562 162 119 241 249 287 3489 Fragments arranged by size: 3489 1562 287 249 241 162 119 BsrBI CCG'CTC Cuts at: 1394 1823 3286 3996 1394 Size: 429 1463 710 3507 Fragments arranged by size: 3507 1463 710 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2784 4604 4778 65 Size: 1183 1536 1820 174 1396 Fragments arranged by size: 1820 1536 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2159 2514 4753 4940 1700 Size: 110 349 355 2239 187 2869 Fragments arranged by size: 2869 2239 355 349 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6109 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6109 BssSI C'ACGA_G Cuts at: 2520 3005 4169 5181 2520 Size: 485 1164 1012 3448 Fragments arranged by size: 3448 1164 1012 485 BstAPI GCAn_nnn'nTGC Cuts at: 794 2488 794 Size: 1694 4415 BstBI TT'CG_AA Cuts at: 4913 4913 Size: 6109 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6109 BstXI CCAn_nnnn'nTGG Cuts at: 2947 2947 Size: 6109 BstYI r'GATC_y Cuts at: 1972 2046 2064 2367 2974 3129 3439 3806 4191 Size: 74 18 303 607 155 310 367 385 Cuts at: 4191 4208 5660 5746 5758 6104 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 607 385 367 346 310 303 155 86 74 18 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4618 Fragments arranged by size: 4618 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3202 487 Size: 683 56 1976 3394 Fragments arranged by size: 3394 1976 683 56 BtsI GCAGTG_nn' Cuts at: 2240 2252 3625 4419 4439 2240 Size: 12 1373 794 20 3910 Fragments arranged by size: 3910 1373 794 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6109 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6074 DraI TTT'AAA Cuts at: 3413 3413 Size: 6109 DraIII CAC_nnn'GTG Cuts at: 2971 2971 Size: 6109 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5116 809 Size: 507 3800 1802 Fragments arranged by size: 3800 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2157 2361 2423 Size: 54 1425 50 275 18 327 204 62 Cuts at: 2423 2781 2835 3102 3556 4445 6074 8 Size: 358 54 267 454 889 1629 43 Fragments arranged by size: 1629 1425 889 454 358 327 275 267 204 62 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2423 3556 1537 Size: 275 611 1133 4090 Fragments arranged by size: 4090 1133 611 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4039 1353 Size: 2686 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2699 4696 5070 5216 387 Size: 1064 138 1110 1997 374 146 1280 Fragments arranged by size: 1997 1280 1110 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2476 2476 Size: 6109 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2089 2372 2548 2553 2674 2897 3311 Size: 393 457 283 176 5 121 223 414 Cuts at: 3311 3332 3356 4172 4769 5556 5824 1239 Size: 21 24 816 597 787 268 1524 Fragments arranged by size: 1524 816 787 597 457 414 393 283 268 223 176 121 24 21 5 EcoNI CCTnn'n_nnAGG Cuts at: 2804 3068 2804 Size: 264 5845 EcoO109I rG'GnC_Cy Cuts at: 2095 2182 2764 2917 2095 Size: 87 582 153 5287 Fragments arranged by size: 5287 582 153 87 EcoRI G'AATT_C Cuts at: 3418 3796 3418 Size: 378 5731 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3148 3148 Size: 6109 FspI TGC'GCA Cuts at: 3269 4615 3269 Size: 1346 4763 HaeII r_GCGC'y Cuts at: 1678 2440 2820 5256 1678 Size: 762 380 2436 2531 Fragments arranged by size: 2531 2436 762 380 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2252 2284 2372 2404 2909 Size: 32 135 32 680 32 88 32 505 Cuts at: 2909 2938 2941 2970 4750 4782 4824 4856 1373 Size: 29 3 29 1780 32 42 32 2626 Fragments arranged by size: 2626 1780 680 505 135 88 42 32 32 32 32 32 32 29 29 3 HincII GTy'rAC Cuts at: 669 1657 1938 3453 3699 669 Size: 988 281 1515 246 3079 Fragments arranged by size: 3079 1515 988 281 246 HindIII A'AGCT_T Cuts at: 748 2010 748 Size: 1262 4847 HpaI GTT'AAC Cuts at: 3699 3699 Size: 6109 KpnI G_GTAC'C Cuts at: 3434 3434 Size: 6109 MfeI C'AATT_G Cuts at: 3708 3708 Size: 6109 MluI A'CGCG_T Cuts at: 5985 5985 Size: 6109 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2358 3219 5223 5407 1305 Size: 1053 861 2004 184 2007 Fragments arranged by size: 2007 2004 1053 861 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2363 2837 3104 10 Size: 54 1425 343 531 474 267 3015 Fragments arranged by size: 3015 1425 531 474 343 267 54 MslI CAynn'nnrTG Cuts at: 518 1166 2245 2547 4067 4426 4585 518 Size: 648 1079 302 1520 359 159 2042 Fragments arranged by size: 2042 1520 1079 648 359 302 159 MspA1I CmG'CkG Cuts at: 1606 2428 2861 2905 3256 3265 3525 4202 5350 Size: 822 433 44 351 9 260 677 1148 Cuts at: 5350 5595 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 822 677 433 351 260 245 44 9 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4618 Fragments arranged by size: 4618 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6109 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6109 NotI GC'GGCC_GC Cuts at: 3556 3556 Size: 6109 NspI r_CATG'y Cuts at: 1132 1229 3467 5012 1132 Size: 97 2238 1545 2229 Fragments arranged by size: 2238 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2185 2449 1348 Size: 837 264 5008 Fragments arranged by size: 5008 837 264 PciI A'CATG_T Cuts at: 5008 5008 Size: 6109 PflMI CCAn_nnn'nTGG Cuts at: 1108 2377 2401 1108 Size: 1269 24 4816 Fragments arranged by size: 4816 1269 24 PfoI T'CCnGG_A Cuts at: 2232 2913 2232 Size: 681 5428 PmeI GTTT'AAAC Cuts at: 3413 3413 Size: 6109 PmlI CAC'GTG Cuts at: 2971 2971 Size: 6109 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4178 4210 5691 5723 4178 Size: 32 1481 32 4564 Fragments arranged by size: 4564 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2917 2917 Size: 6109 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6109 PsiI TTA'TAA Cuts at: 3679 3679 Size: 6109 PspXI vC'TCGA_Gb Cuts at: 2408 2408 Size: 6109 PstI C_TGCA'G Cuts at: 830 2178 2247 3461 830 Size: 1348 69 1214 3478 Fragments arranged by size: 3478 1348 1214 69 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6109 PvuII CAG'CTG Cuts at: 3256 3265 3256 Size: 9 6100 SacI G_AGCT'C Cuts at: 2478 2478 Size: 6109 SalI G'TCGA_C Cuts at: 1936 3451 1936 Size: 1515 4594 SbfI CC_TGCA'GG Cuts at: 3461 3461 Size: 6109 ScaI AGT'ACT Cuts at: 4357 6069 4357 Size: 1712 4397 SexAI A'CCwGG_T Cuts at: 2052 2052 Size: 6109 SfcI C'TryA_G Cuts at: 826 1045 2174 2243 3140 3457 4592 5273 5464 Size: 219 1129 69 897 317 1135 681 191 Cuts at: 5464 826 Size: 1471 Fragments arranged by size: 1471 1135 1129 897 681 317 219 191 69 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6109 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6109 SmaI CCC'GGG Cuts at: 1872 3436 1872 Size: 1564 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2408 2477 3133 3320 4213 5114 Size: 197 926 465 69 656 187 893 901 Cuts at: 5114 5376 820 Size: 262 1553 Fragments arranged by size: 1553 926 901 893 656 465 262 197 187 69 SnaBI TAC'GTA Cuts at: 493 3900 493 Size: 3407 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6109 SphI G_CATG'C Cuts at: 1132 3467 1132 Size: 2335 3774 SspI AAT'ATT Cuts at: 5 52 4033 5 Size: 47 3981 2081 Fragments arranged by size: 3981 2081 47 StuI AGG'CCT Cuts at: 1517 2264 1517 Size: 747 5362 StyI C'CwwG_G Cuts at: 513 1065 2004 3099 3548 513 Size: 552 939 1095 449 3074 Fragments arranged by size: 3074 1095 939 552 449 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4136 4153 4306 4491 4936 325 Size: 1255 2556 17 153 185 445 1498 Fragments arranged by size: 2556 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3114 4355 6067 Size: 274 80 33 51 1443 1137 1241 1712 Cuts at: 6067 96 Size: 138 Fragments arranged by size: 1712 1443 1241 1137 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6069 6101 6069 Size: 32 6077 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6109 XbaI T'CTAG_A Cuts at: 3445 3445 Size: 6109 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2791 2791 Size: 6109 XhoI C'TCGA_G Cuts at: 1943 2408 1943 Size: 465 5644 XmaI C'CCGG_G Cuts at: 1870 3434 1870 Size: 1564 4545 XmnI GAAnn'nnTTC Cuts at: 2030 3239 4238 2030 Size: 1209 999 3901 Fragments arranged by size: 3901 1209 999 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3917 276 Size: 53 83 186 936 2383 2468 Fragments arranged by size: 2468 2383 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI