Restriction map for
pFN21AE3916
(Linear) (Six-base) MAPSORT of: KIEE3916.seq Check: 7981 from: 1 to: 1404
KIEE3916.orf
With 182 enzymes: SgfI *
March 22, 2012 16:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 401 563 682 1404
Size: 401 162 119 722
Fragments arranged by size:
722 401 162 119
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 1404
Size: 367 1037
AfeI AGC'GCT
Cuts at: 0 433 1404
Size: 433 971
AflII C'TTAA_G
Cuts at: 0 1315 1404
Size: 1315 89
AleI CACnn'nnGTG
Cuts at: 0 240 542 1404
Size: 240 302 862
Fragments arranged by size:
862 302 240
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 632 1404
Size: 632 772
AlwNI CAG_nnn'CTG
Cuts at: 0 128 483 555 582 762 915 1164 1404
Size: 128 355 72 27 180 153 249 240
Fragments arranged by size:
355 249 240 180 153 128 72 27
AvaI C'yCGr_G
Cuts at: 0 403 1404
Size: 403 1001
BanI G'GyrC_C
Cuts at: 0 321 821 1404
Size: 321 500 583
Fragments arranged by size:
583 500 321
BanII G_rGCy'C
Cuts at: 0 286 473 1404
Size: 286 187 931
Fragments arranged by size:
931 286 187
BbsI GAAGACnn'nnnn_
Cuts at: 0 347 1404
Size: 347 1057
BbvCI CC'TCA_GC
Cuts at: 0 214 424 462 1404
Size: 214 210 38 942
Fragments arranged by size:
942 214 210 38
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 595 629 1354 1388 1404
Size: 595 34 725 34 16
Fragments arranged by size:
725 595 34 34 16
BglII A'GATC_T
Cuts at: 0 59 1124 1404
Size: 59 1065 280
Fragments arranged by size:
1065 280 59
BlpI GC'TnA_GC
Cuts at: 0 280 586 1292 1404
Size: 280 306 706 112
Fragments arranged by size:
706 306 280 112
Bme1580I G_kGCm'C
Cuts at: 0 326 461 1404
Size: 326 135 943
Fragments arranged by size:
943 326 135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 543 548 669 892 1306 1327 1351
Size: 84 459 5 121 223 414 21 24
Cuts at: 1351 1404
Size: 53
Fragments arranged by size:
459 414 223 121 84 53 24 21
5
Bpu10I CC'TnA_GC
Cuts at: 0 214 424 462 1404
Size: 214 210 38 942
Fragments arranged by size:
942 214 210 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 1149 1404
Size: 457 692 255
Fragments arranged by size:
692 457 255
BsaI GGTCTCn'nnnn_
Cuts at: 0 835 1404
Size: 835 569
BsaAI yAC'GTr
Cuts at: 0 966 1404
Size: 966 438
BsaBI GATnn'nnATC
Cuts at: 0 1210 1404
Size: 1210 194
BsaWI w'CCGG_w
Cuts at: 0 11 644 1404
Size: 11 633 760
Fragments arranged by size:
760 633 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 814 817 820 1404
Size: 814 3 3 584
Fragments arranged by size:
814 584 3 3
BseYI C'CCAG_C
Cuts at: 0 250 396 434 1404
Size: 250 146 38 970
Fragments arranged by size:
970 250 146 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 1404
Size: 541 863
BsiEI CG_ry'CG
Cuts at: 0 421 1404
Size: 421 983
BsiHKAI G_wGCw'C
Cuts at: 0 205 473 611 908 1404
Size: 205 268 138 297 496
Fragments arranged by size:
496 297 268 205 138
Bsp1286I G_dGCh'C
Cuts at: 0 205 286 326 461 473 611 908 1404
Size: 205 81 40 135 12 138 297 496
Fragments arranged by size:
496 297 205 138 135 81 40 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 401 563 682 923 1172 1404
Size: 401 162 119 241 249 232
Fragments arranged by size:
401 249 241 232 162 119
BsrBI CCG'CTC
Cuts at: 0 1281 1404
Size: 1281 123
BsrDI GCAATG_nn'
Cuts at: 0 779 1404
Size: 779 625
BsrFI r'CCGG_y
Cuts at: 0 154 509 1404
Size: 154 355 895
Fragments arranged by size:
895 355 154
BssSI C'ACGA_G
Cuts at: 0 515 1000 1404
Size: 515 485 404
Fragments arranged by size:
515 485 404
BstAPI GCAn_nnn'nTGC
Cuts at: 0 483 1404
Size: 483 921
BstXI CCAn_nnnn'nTGG
Cuts at: 0 942 1404
Size: 942 462
BstYI r'GATC_y
Cuts at: 0 41 59 362 969 1124 1404
Size: 41 18 303 607 155 280
Fragments arranged by size:
607 303 280 155 41 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1197 1404
Size: 1197 207
BtsI GCAGTG_nn'
Cuts at: 0 235 247 1404
Size: 235 12 1157
Fragments arranged by size:
1157 235 12
DraIII CAC_nnn'GTG
Cuts at: 0 966 1404
Size: 966 438
EaeI y'GGCC_r
Cuts at: 0 152 356 418 776 830 1097 1404
Size: 152 204 62 358 54 267 307
Fragments arranged by size:
358 307 267 204 152 62 54
EagI C'GGCC_G
Cuts at: 0 418 1404
Size: 418 986
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 694 1404
Size: 694 710
EcoICRI GAG'CTC
Cuts at: 0 471 1404
Size: 471 933
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 367 543 548 669 892 1306 1327
Size: 84 283 176 5 121 223 414 21
Cuts at: 1327 1351 1404
Size: 24 53
Fragments arranged by size:
414 283 223 176 121 84 53 24
21 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 799 1063 1404
Size: 799 264 341
Fragments arranged by size:
799 341 264
EcoO109I rG'GnC_Cy
Cuts at: 0 90 177 759 912 1404
Size: 90 87 582 153 492
Fragments arranged by size:
582 492 153 90 87
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1143 1404
Size: 1143 261
FspI TGC'GCA
Cuts at: 0 1264 1404
Size: 1264 140
HaeII r_GCGC'y
Cuts at: 0 435 815 1404
Size: 435 380 589
Fragments arranged by size:
589 435 380
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 247 279 367 399 904 933 936 965
Size: 247 32 88 32 505 29 3 29
Cuts at: 965 1404
Size: 439
Fragments arranged by size:
505 439 247 88 32 32 29 29
3
HindIII A'AGCT_T
Cuts at: 0 5 1404
Size: 5 1399
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 1214 1404
Size: 353 861 190
Fragments arranged by size:
861 353 190
MscI TGG'CCA
Cuts at: 0 358 832 1099 1404
Size: 358 474 267 305
Fragments arranged by size:
474 358 305 267
MslI CAynn'nnrTG
Cuts at: 0 240 542 1404
Size: 240 302 862
Fragments arranged by size:
862 302 240
MspA1I CmG'CkG
Cuts at: 0 423 856 900 1251 1260 1404
Size: 423 433 44 351 9 144
Fragments arranged by size:
433 423 351 144 44 9
PasI CC'CwG_GG
Cuts at: 0 180 444 1404
Size: 180 264 960
Fragments arranged by size:
960 264 180
PflMI CCAn_nnn'nTGG
Cuts at: 0 372 396 1404
Size: 372 24 1008
Fragments arranged by size:
1008 372 24
PfoI T'CCnGG_A
Cuts at: 0 227 908 1404
Size: 227 681 496
Fragments arranged by size:
681 496 227
PmlI CAC'GTG
Cuts at: 0 966 1404
Size: 966 438
PpuMI rG'GwC_Cy
Cuts at: 0 912 1404
Size: 912 492
PspXI vC'TCGA_Gb
Cuts at: 0 403 1404
Size: 403 1001
PstI C_TGCA'G
Cuts at: 0 173 242 1404
Size: 173 69 1162
Fragments arranged by size:
1162 173 69
PvuII CAG'CTG
Cuts at: 0 1251 1260 1404
Size: 1251 9 144
Fragments arranged by size:
1251 144 9
SacI G_AGCT'C
Cuts at: 0 473 1404
Size: 473 931
SexAI A'CCwGG_T
Cuts at: 0 47 1404
Size: 47 1357
SfcI C'TryA_G
Cuts at: 0 169 238 1135 1404
Size: 169 69 897 269
Fragments arranged by size:
897 269 169 69
SmlI C'TyrA_G
Cuts at: 0 403 472 1128 1315 1404
Size: 403 69 656 187 89
Fragments arranged by size:
656 403 187 89 69
StuI AGG'CCT
Cuts at: 0 259 1404
Size: 259 1145
StyI C'CwwG_G
Cuts at: 0 1094 1404
Size: 1094 310
TatI w'GTAC_w
Cuts at: 0 1109 1404
Size: 1109 295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 786 1404
Size: 786 618
XhoI C'TCGA_G
Cuts at: 0 403 1404
Size: 403 1001
XmnI GAAnn'nnTTC
Cuts at: 0 25 1234 1404
Size: 25 1209 170
Fragments arranged by size:
1209 170 25
Enzymes that do cut:
AarI AcuI AfeI AflII AleI AlfI AlwNI AvaI
BanI BanII BbsI BbvCI BcgI BglII BlpI Bme1580I
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstXI BstYI BtgZI BtsI DraIII
EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I HindIII MmeI MscI MslI MspA1I
PasI PflMI PfoI PmlI PpuMI PspXI PstI PvuII
SacI SexAI SfcI SmlI StuI StyI TatI XcmI
XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflIII AgeI AhdI AloI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BciVI BclI BfrBI BglI BmgBI BmrI
BmtI BplI BsaHI BsaXI BsiWI BsmI BsmBI BspEI
BspHI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgI
ClaI CspCI DraI DrdI EarI EcoRI EcoRV FseI
FspAI HincII HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PmeI PpiI PshAI PsiI PspOMI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TstI Tth111I XbaI
XmaI ZraI