(Circular) (Six-base) MAPSORT of: pFN21AE5609.seq Check: 6612 from: 1 to: 6304 pFN21AE5609 6304 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2119 2119 Size: 6304 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4114 278 Size: 53 83 186 936 2578 2468 Fragments arranged by size: 2578 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2793 3647 1937 Size: 856 854 4594 Fragments arranged by size: 4594 856 854 Acc65I G'GTAC_C Cuts at: 3625 3625 Size: 6304 AclI AA'CG_TT Cuts at: 1563 4431 4804 1563 Size: 2868 373 3063 Fragments arranged by size: 3063 2868 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4367 5751 6019 1632 Size: 2735 1384 268 1917 Fragments arranged by size: 2735 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6107 AflIII A'CryG_T Cuts at: 1203 5203 6180 1203 Size: 4000 977 1327 Fragments arranged by size: 4000 1327 977 AgeI A'CCGG_T Cuts at: 5135 5135 Size: 6304 AhdI GACnn_n'nnGTC Cuts at: 5033 5033 Size: 6304 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6304 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6304 AlwNI CAG_nnn'CTG Cuts at: 4047 5619 4047 Size: 1572 4732 ApaLI G'TGCA_C Cuts at: 4361 5517 4361 Size: 1156 5148 ApoI r'AATT_y Cuts at: 1493 2084 2562 3092 3525 3613 3839 3991 6100 Size: 591 478 530 433 88 226 152 2109 Cuts at: 6100 1493 Size: 1697 Fragments arranged by size: 2109 1697 591 530 478 433 226 152 88 AseI AT'TA_AT Cuts at: 160 1032 3338 4858 160 Size: 872 2306 1520 1606 Fragments arranged by size: 2306 1606 1520 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6304 AvaI C'yCGr_G Cuts at: 1870 1943 3629 1870 Size: 73 1686 4545 Fragments arranged by size: 4545 1686 73 BamHI G'GATC_C Cuts at: 3596 3634 4001 3596 Size: 38 367 5899 Fragments arranged by size: 5899 367 38 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2107 3625 5080 618 Size: 325 217 627 320 1518 1455 1842 Fragments arranged by size: 1842 1518 1455 627 325 320 217 BanII G_rGCy'C Cuts at: 1391 2055 3623 1391 Size: 664 1568 4072 Fragments arranged by size: 4072 1568 664 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5333 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4495 4529 1903 Size: 34 2558 34 3678 Fragments arranged by size: 3678 2558 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3180 4196 5406 3180 Size: 1016 1210 4078 Fragments arranged by size: 4078 1210 1016 BclI T'GATC_A Cuts at: 1548 2866 1548 Size: 1318 4986 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4915 136 Size: 107 122 71 631 605 3243 1525 Fragments arranged by size: 3243 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2935 6299 2935 Size: 3364 2940 BlpI GC'TnA_GC Cuts at: 3721 3721 Size: 6304 Bme1580I G_kGCm'C Cuts at: 1790 4365 5521 1790 Size: 2575 1156 2573 Fragments arranged by size: 2575 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6304 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4035 4993 442 Size: 416 53 83 399 24 2618 958 1753 Fragments arranged by size: 2618 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6304 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4964 1239 Size: 3725 2579 Bpu10I CC'TnA_GC Cuts at: 2501 2826 2501 Size: 325 5979 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2916 4429 5294 5592 2916 Size: 1513 865 298 3628 Fragments arranged by size: 3628 1513 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4967 882 Size: 617 3468 2219 Fragments arranged by size: 3468 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3236 4095 493 Size: 711 2032 859 2702 Fragments arranged by size: 2702 2032 859 711 BsaBI GATnn'nnATC Cuts at: 6298 6298 Size: 6304 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4111 4493 5128 275 Size: 53 83 186 936 2578 382 635 1451 Fragments arranged by size: 2578 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4737 5135 5409 5556 5967 4737 Size: 398 274 147 411 5074 Fragments arranged by size: 5074 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6079 Fragments arranged by size: 6079 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 1186 Size: 931 5373 BseYI C'CCAG_C Cuts at: 5507 6003 5507 Size: 496 5808 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3404 1163 Size: 573 1668 4063 Fragments arranged by size: 4063 1668 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3754 4515 5543 1540 Size: 275 186 1753 761 1028 2301 Fragments arranged by size: 2301 1753 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2055 4365 4450 5521 2055 Size: 2310 85 1071 2838 Fragments arranged by size: 2838 2310 1071 85 BsmI GAATG_Cn' Cuts at: 2206 3815 3908 2206 Size: 1609 93 4602 Fragments arranged by size: 4602 1609 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 3623 4365 4450 5521 1391 Size: 399 265 1568 742 85 1071 2174 Fragments arranged by size: 2174 1568 1071 742 399 265 85 BspEI T'CCGG_A Cuts at: 5967 5967 Size: 6304 BspHI T'CATG_A Cuts at: 2856 4193 5923 2856 Size: 1337 1730 3237 Fragments arranged by size: 3237 1730 1337 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2119 3659 844 Size: 1275 1540 3489 Fragments arranged by size: 3489 1540 1275 BsrBI CCG'CTC Cuts at: 1394 1823 4191 1394 Size: 429 2368 3507 Fragments arranged by size: 3507 2368 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4799 4973 65 Size: 1183 3551 174 1396 Fragments arranged by size: 3551 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4948 5135 1700 Size: 110 3138 187 2869 Fragments arranged by size: 3138 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6304 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6304 BssSI C'ACGA_G Cuts at: 4364 5376 4364 Size: 1012 5292 BstAPI GCAn_nnn'nTGC Cuts at: 794 3140 794 Size: 2346 3958 BstBI TT'CG_AA Cuts at: 3398 5108 3398 Size: 1710 4594 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6304 BstYI r'GATC_y Cuts at: 1972 2935 3596 3634 4001 4386 4403 5855 5941 Size: 963 661 38 367 385 17 1452 86 Cuts at: 5941 5953 6299 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 963 661 385 367 346 86 38 17 12 BtgI C'CryG_G Cuts at: 513 1065 3546 513 Size: 552 2481 3271 Fragments arranged by size: 3271 2481 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5565 Fragments arranged by size: 5565 683 56 BtsI GCAGTG_nn' Cuts at: 2812 3820 4614 4634 2812 Size: 1008 794 20 4482 Fragments arranged by size: 4482 1008 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6304 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6269 DraI TTT'AAA Cuts at: 2089 3608 2089 Size: 1519 4785 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5311 809 Size: 507 3995 1802 Fragments arranged by size: 3995 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3751 4640 6269 Size: 54 1425 50 275 18 1921 889 1629 Cuts at: 6269 8 Size: 43 Fragments arranged by size: 1921 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3751 1537 Size: 275 1939 4090 Fragments arranged by size: 4090 1939 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3116 4234 1353 Size: 1763 1118 3423 Fragments arranged by size: 3423 1763 1118 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4891 5265 5411 387 Size: 1064 138 3302 374 146 1280 Fragments arranged by size: 3302 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2053 2053 Size: 6304 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4367 4964 5751 6019 1239 Size: 393 2735 597 787 268 1524 Fragments arranged by size: 2735 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 3613 3991 3613 Size: 378 5926 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2739 3292 2739 Size: 553 5751 FspI TGC'GCA Cuts at: 4810 4810 Size: 6304 HaeII r_GCGC'y Cuts at: 1678 5451 1678 Size: 3773 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2851 2883 3174 3206 3410 Size: 32 135 32 1279 32 291 32 204 Cuts at: 3410 3442 3514 3546 4945 4977 5019 5051 1373 Size: 32 72 32 1399 32 42 32 2626 Fragments arranged by size: 2626 1399 1279 291 204 135 72 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3648 3894 669 Size: 988 281 1710 246 3079 Fragments arranged by size: 3079 1710 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6304 HpaI GTT'AAC Cuts at: 3894 3894 Size: 6304 KpnI G_GTAC'C Cuts at: 3629 3629 Size: 6304 MfeI C'AATT_G Cuts at: 2211 3421 3903 2211 Size: 1210 482 4612 Fragments arranged by size: 4612 1210 482 MluI A'CGCG_T Cuts at: 6180 6180 Size: 6304 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2048 5418 5602 1305 Size: 743 3370 184 2007 Fragments arranged by size: 3370 2007 743 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4482 Fragments arranged by size: 4482 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2447 4262 4621 4780 518 Size: 648 1281 1815 359 159 2042 Fragments arranged by size: 2042 1815 1281 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3720 4397 5545 5790 1606 Size: 2114 677 1148 245 2120 Fragments arranged by size: 2120 2114 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5752 NdeI CA'TA_TG Cuts at: 387 2179 387 Size: 1792 4512 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6304 NotI GC'GGCC_GC Cuts at: 3751 3751 Size: 6304 NspI r_CATG'y Cuts at: 1132 1229 3662 5207 1132 Size: 97 2433 1545 2229 Fragments arranged by size: 2433 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6304 PciI A'CATG_T Cuts at: 5203 5203 Size: 6304 PflMI CCAn_nnn'nTGG Cuts at: 1108 2711 1108 Size: 1603 4701 PmeI GTTT'AAAC Cuts at: 3608 3608 Size: 6304 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4373 4405 5886 5918 4373 Size: 32 1481 32 4759 Fragments arranged by size: 4759 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6304 PsiI TTA'TAA Cuts at: 3197 3874 3197 Size: 677 5627 PstI C_TGCA'G Cuts at: 830 3656 830 Size: 2826 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6304 SacI G_AGCT'C Cuts at: 2055 2055 Size: 6304 SalI G'TCGA_C Cuts at: 1936 3646 1936 Size: 1710 4594 SbfI CC_TGCA'GG Cuts at: 3656 3656 Size: 6304 ScaI AGT'ACT Cuts at: 4552 6264 4552 Size: 1712 4592 SfcI C'TryA_G Cuts at: 826 1045 2728 3652 4787 5468 5659 826 Size: 219 1683 924 1135 681 191 1471 Fragments arranged by size: 1683 1471 1135 924 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6304 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6304 SmaI CCC'GGG Cuts at: 1872 3631 1872 Size: 1759 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2931 4408 5309 5571 820 Size: 197 926 988 1477 901 262 1553 Fragments arranged by size: 1553 1477 988 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4095 493 Size: 3602 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6304 SphI G_CATG'C Cuts at: 1132 3662 1132 Size: 2530 3774 SspI AAT'ATT Cuts at: 5 52 2509 3336 4228 5 Size: 47 2457 827 892 2081 Fragments arranged by size: 2457 2081 892 827 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6304 StyI C'CwwG_G Cuts at: 513 1065 2907 3743 513 Size: 552 1842 836 3074 Fragments arranged by size: 3074 1842 836 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4331 4348 4501 4686 5131 325 Size: 1255 2751 17 153 185 445 1498 Fragments arranged by size: 2751 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4550 6262 96 Size: 274 80 33 51 1443 2573 1712 138 Fragments arranged by size: 2573 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6264 6296 6264 Size: 32 6272 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6304 XbaI T'CTAG_A Cuts at: 2761 3640 2761 Size: 879 5425 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6304 XmaI C'CCGG_G Cuts at: 1870 3629 1870 Size: 1759 4545 XmnI GAAnn'nnTTC Cuts at: 3093 3301 4433 3093 Size: 208 1132 4964 Fragments arranged by size: 4964 1132 208 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4112 276 Size: 53 83 186 936 2578 2468 Fragments arranged by size: 2578 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI