################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AvaI AvaII AviII AvrII BamHI BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfrI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HindIII HpaI KasI KspI MamI MfeI MluI MroI MseI MslI MunI NarI NciI NcoI NdeI NheI NotI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I Tsp45I TthHB8I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### Acc65I 88 GGTACC G^GTACC AccI 439 GTCTAC GT^[AC][GT]AC AccII 65 CGCG CG^CG AcyI 562 GACGCC G[GA]^CG[CT]C AlwI 146 GGATC GGATC(4/5) Asp718I 88 GGTACC G^GTACC AspHI 226 GAGCTC G[AT]GC[AT]^C AspI 154 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 542 TGGCCA TGG^CCA BanI 88 GGTACC G^G[CT][GA]CC BanII 226 GAGCTC G[GA]GC[CT]^C BbvCI 549 CCTCAGC CCTCAGC(-5/-2) BglI 19 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 226 GAGCTC G[AGT]GC[ACT]^C BsaHI 562 GACGCC G[GA]^CG[CT]C BsgI 516 CTGCAC GTGCAG(16/14) BsiHKAI 226 GAGCTC G[AT]GC[AT]^C BsmFI 455 GGGAC GGGAC(10/14) Bsp1286I 226 GAGCTC G[AGT]GC[ACT]^C BstUI 65 CGCG CG^CG CfoI 474 GCGC GCG^C DdeI 550 CTCAG C^T[ACGT]AG DsaI 71 CCGTGG C^C[GA][CT]GG EarI 127 CTCTTC CTCTTC(1/4) EcoT14I 305 CCTTGG C^C[AT][AT]GG HhaI 474 GCGC GCG^C Hin1I 562 GACGCC G[GA]^CG[CT]C HinP1I 474 GCGC G^CGC HincII 160 GTCAAC GT[CT]^[GA]AC HindII 160 GTCAAC GT[CT]^[GA]AC HinfI 457 GACTC G^A[ACGT]TC KpnI 88 GGTACC GGTAC^C Ksp632I 127 CTCTTC CTCTTC(1/4) MaeII 212 ACGT A^CGT MaeIII 209 GTAAC ^GT[ACGT]AC MluNI 542 TGGCCA TGG^CCA MscI 542 TGGCCA TGG^CCA MvnI 65 CGCG CG^CG NaeI 15 GCCGGC GCC^GGC NgoMIV 15 GCCGGC G^CCGGC NruI 64 TCGCGA TCG^CGA PflFI 154 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 521 CCATCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 457 GACTC GAGTC(4/5) PvuII 400 CAGCTG CAG^CTG SacI 226 GAGCTC GAGCT^C SfaNI 396 GATGC GCATC(5/9) SmlI 459 CTCAAG C^T[CT][GA]AG StuI 417 AGGCCT AGG^CCT StyI 305 CCTTGG C^C[AT][AT]GG Tsp509I 8 AATT ^AATT TspRI 514 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 154 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 521 CCATCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 271 TCTAGA T^CTAGA XcmI 290 CCATGTTCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### BfaI 250 CTAG C^TAG 272 CTAG BglII 35 AGATCT A^GATCT 383 AGATCT BsiYI 169 CCGCAGGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 521 CCATCCGCTGG BslI 169 CCGCAGGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 521 CCATCCGCTGG BsrFI 15 GCCGGC [GA]^CCGG[CT] 602 ACCGGC BsrI 114 ACTGG ACTGG(1/-1) 143 ACTGG BstYI 35 AGATCT [GA]^GATC[CT] 383 AGATCT Cfr10I 15 GCCGGC [GA]^CCGG[CT] 602 ACCGGC HapII 16 CCGG C^CGG 603 CCGG HgaI 562 GACGC GACGC(5/10) 158 GCGTC HpaII 16 CCGG C^CGG 603 CCGG HphI 3 GGTGA GGTGA(8/7) 95 TCACC MaeI 250 CTAG C^TAG 272 CTAG MboII 128 TCTTC GAAGA(8/7) 353 TCTTC MflI 35 AGATCT [GA]^GATC[CT] 383 AGATCT MspA1I 400 CAGCTG C[AC]G^C[GT]G 525 CCGCTG MspI 16 CCGG C^CGG 603 CCGG NspI 311 GCATGT [GA]CATG^[CT] 476 GCATGT XhoII 35 AGATCT [GA]^GATC[CT] 383 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AciI AfaI AluI BbvI BsaJI BseRI BssKI BstF5I BstNI Cac8I Cfr13I DpnII EaeI EcoRII Fnu4HI FokI HaeIII ItaI MboI MnlI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TseI