Restriction map for
pFN21AE6227
(Linear) (Six-base) MAPSORT of: KIEE6227.seq Check: 6312 from: 1 to: 657
KIEE6227.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AccI GT'mk_AC
Cuts at: 0 440 657
Size: 440 217
Acc65I G'GTAC_C
Cuts at: 0 88 657
Size: 88 569
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 327 657
Size: 327 330
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 422 657
Size: 422 235
BanI G'GyrC_C
Cuts at: 0 88 657
Size: 88 569
BanII G_rGCy'C
Cuts at: 0 230 657
Size: 230 427
BbvCI CC'TCA_GC
Cuts at: 0 550 657
Size: 550 107
BglI GCCn_nnn'nGGC
Cuts at: 0 25 657
Size: 25 632
BglII A'GATC_T
Cuts at: 0 35 383 657
Size: 35 348 274
Fragments arranged by size:
348 274 35
Bpu10I CC'TnA_GC
Cuts at: 0 550 657
Size: 550 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 444 657
Size: 444 213
BsaHI Gr'CG_yC
Cuts at: 0 563 657
Size: 563 94
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 244 274 657
Size: 244 30 383
Fragments arranged by size:
383 244 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 249 607 657
Size: 249 358 50
Fragments arranged by size:
358 249 50
BseYI C'CCAG_C
Cuts at: 0 507 527 657
Size: 507 20 130
Fragments arranged by size:
507 130 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 501 657
Size: 501 156
BsiHKAI G_wGCw'C
Cuts at: 0 230 657
Size: 230 427
Bsp1286I G_dGCh'C
Cuts at: 0 230 657
Size: 230 427
BsrFI r'CCGG_y
Cuts at: 0 15 602 657
Size: 15 587 55
Fragments arranged by size:
587 55 15
BstYI r'GATC_y
Cuts at: 0 35 383 657
Size: 35 348 274
Fragments arranged by size:
348 274 35
BtgI C'CryG_G
Cuts at: 0 71 657
Size: 71 586
BtsI GCAGTG_nn'
Cuts at: 0 513 657
Size: 513 144
EaeI y'GGCC_r
Cuts at: 0 368 410 542 657
Size: 368 42 132 115
Fragments arranged by size:
368 132 115 42
EarI CTCTTCn'nnn_
Cuts at: 0 133 657
Size: 133 524
EcoICRI GAG'CTC
Cuts at: 0 228 657
Size: 228 429
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 327 657
Size: 327 330
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 244 276 657
Size: 244 32 381
Fragments arranged by size:
381 244 32
HincII GTy'rAC
Cuts at: 0 162 657
Size: 162 495
KpnI G_GTAC'C
Cuts at: 0 92 657
Size: 92 565
MscI TGG'CCA
Cuts at: 0 544 657
Size: 544 113
MspA1I CmG'CkG
Cuts at: 0 402 527 657
Size: 402 125 130
Fragments arranged by size:
402 130 125
NaeI GCC'GGC
Cuts at: 0 17 657
Size: 17 640
NgoMIV G'CCGG_C
Cuts at: 0 15 657
Size: 15 642
NruI TCG'CGA
Cuts at: 0 66 657
Size: 66 591
NspI r_CATG'y
Cuts at: 0 315 480 657
Size: 315 165 177
Fragments arranged by size:
315 177 165
PflMI CCAn_nnn'nTGG
Cuts at: 0 527 657
Size: 527 130
PvuII CAG'CTG
Cuts at: 0 402 657
Size: 402 255
SacI G_AGCT'C
Cuts at: 0 230 657
Size: 230 427
SmlI C'TyrA_G
Cuts at: 0 459 657
Size: 459 198
StuI AGG'CCT
Cuts at: 0 419 657
Size: 419 238
StyI C'CwwG_G
Cuts at: 0 305 657
Size: 305 352
TatI w'GTAC_w
Cuts at: 0 500 657
Size: 500 157
Tth111I GACn'n_nGTC
Cuts at: 0 157 657
Size: 157 500
XbaI T'CTAG_A
Cuts at: 0 271 657
Size: 271 386
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 297 657
Size: 297 360
Enzymes that do cut:
AccI Acc65I AcuI AlfI BanI BanII BbvCI BglI
BglII Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BsrFI BstYI BtgI BtsI EaeI EarI
EcoICRI Eco57MI Hin4I HincII KpnI MscI MspA1I NaeI
NgoMIV NruI NspI PflMI PvuII SacI SmlI StuI
StyI TatI Tth111I XbaI XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BcgI
BciVI BclI BfrBI BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HaeII HindIII HpaI KasI MfeI MluI MmeI MslI
NarI NcoI NdeI NheI NotI NsiI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TstI XhoI XmaI XmnI ZraI