(Circular) (Six-base) MAPSORT of: pFN21AE4495.seq Check: 8447 from: 1 to: 6262 pFN21AE4495 6262 bp With 182 enzymes: SgfI * March 28, 2012 13:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4072 278 Size: 53 83 186 936 2536 2468 Fragments arranged by size: 2536 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3605 1937 Size: 1668 4594 Acc65I G'GTAC_C Cuts at: 2667 3583 2667 Size: 916 5346 AclI AA'CG_TT Cuts at: 1563 4389 4762 1563 Size: 2826 373 3063 Fragments arranged by size: 3063 2826 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3022 4325 5709 5977 1632 Size: 1390 1303 1384 268 1917 Fragments arranged by size: 1917 1390 1384 1303 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6065 AflIII A'CryG_T Cuts at: 1203 5161 6138 1203 Size: 3958 977 1327 Fragments arranged by size: 3958 1327 977 AgeI A'CCGG_T Cuts at: 2345 2664 5093 2345 Size: 319 2429 3514 Fragments arranged by size: 3514 2429 319 AhdI GACnn_n'nnGTC Cuts at: 4991 4991 Size: 6262 AleI CACnn'nnGTG Cuts at: 1166 2192 1166 Size: 1026 5236 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2072 2210 2975 1184 Size: 888 138 765 4471 Fragments arranged by size: 4471 888 765 138 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2381 2413 2381 Size: 32 6230 AlwNI CAG_nnn'CTG Cuts at: 3265 4005 5577 3265 Size: 740 1572 3950 Fragments arranged by size: 3950 1572 740 ApaI G_GGCC'C Cuts at: 2511 2511 Size: 6262 ApaLI G'TGCA_C Cuts at: 2185 2293 4319 5475 2185 Size: 108 2026 1156 2972 Fragments arranged by size: 2972 2026 1156 108 ApoI r'AATT_y Cuts at: 1493 2270 3571 3797 3949 6058 1493 Size: 777 1301 226 152 2109 1697 Fragments arranged by size: 2109 1697 1301 777 226 152 AseI AT'TA_AT Cuts at: 160 1032 4816 160 Size: 872 3784 1606 Fragments arranged by size: 3784 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6262 AvaI C'yCGr_G Cuts at: 1870 1943 2082 3587 1870 Size: 73 139 1505 4545 Fragments arranged by size: 4545 1505 139 73 BamHI G'GATC_C Cuts at: 3592 3959 3592 Size: 367 5895 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2432 2661 2667 3583 5038 Size: 325 217 627 645 229 6 916 1455 Cuts at: 5038 618 Size: 1842 Fragments arranged by size: 1842 1455 916 645 627 325 229 217 6 BanII G_rGCy'C Cuts at: 1391 2012 2068 2511 3581 1391 Size: 621 56 443 1070 4072 Fragments arranged by size: 4072 1070 621 443 56 BbeI G_GCGC'C Cuts at: 2436 2436 Size: 6262 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3499 928 Size: 971 1600 3691 Fragments arranged by size: 3691 1600 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4453 4487 1903 Size: 34 2516 34 3678 Fragments arranged by size: 3678 2516 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4154 5364 4154 Size: 1210 5052 BclI T'GATC_A Cuts at: 1548 2475 1548 Size: 927 5335 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4873 136 Size: 107 122 71 631 605 3201 1525 Fragments arranged by size: 3201 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2440 2692 6257 2440 Size: 252 3565 2445 Fragments arranged by size: 3565 2445 252 BlpI GC'TnA_GC Cuts at: 3679 3679 Size: 6262 Bme1580I G_kGCm'C Cuts at: 1790 2189 2297 2511 4323 5479 1790 Size: 399 108 214 1812 1156 2573 Fragments arranged by size: 2573 1812 1156 399 214 108 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6262 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2760 3993 4951 Size: 416 53 83 399 24 1343 1233 958 Cuts at: 4951 442 Size: 1753 Fragments arranged by size: 1753 1343 1233 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6262 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2236 2681 2827 2981 3300 3387 4922 1239 Size: 997 445 146 154 319 87 1535 2579 Fragments arranged by size: 2579 1535 997 445 319 154 146 87 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2422 2818 3078 4387 5252 5550 2422 Size: 396 260 1309 865 298 3134 Fragments arranged by size: 3134 1309 865 396 298 260 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2794 4925 882 Size: 617 1295 2131 2219 Fragments arranged by size: 2219 2131 1295 617 BsaAI yAC'GTr Cuts at: 493 1204 2473 4053 493 Size: 711 1269 1580 2702 Fragments arranged by size: 2702 1580 1269 711 BsaBI GATnn'nnATC Cuts at: 6256 6256 Size: 6262 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2433 2608 4069 4451 Size: 53 83 186 936 900 175 1461 382 Cuts at: 4451 5086 275 Size: 635 1451 Fragments arranged by size: 1461 1451 936 900 635 382 186 175 83 53 BsaWI w'CCGG_w Cuts at: 2280 2345 2664 4695 5093 5367 5514 5925 2280 Size: 65 319 2031 398 274 147 411 2617 Fragments arranged by size: 2617 2031 411 398 319 274 147 65 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6037 Fragments arranged by size: 6037 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6262 BseYI C'CCAG_C Cuts at: 2494 2520 2847 5465 5961 2494 Size: 26 327 2618 496 2795 Fragments arranged by size: 2795 2618 496 327 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2160 1163 Size: 573 424 5265 Fragments arranged by size: 5265 573 424 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3712 4473 5501 1540 Size: 275 186 1711 761 1028 2301 Fragments arranged by size: 2301 1711 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2189 2297 4323 4408 5479 2189 Size: 108 2026 85 1071 2972 Fragments arranged by size: 2972 2026 1071 108 85 BsmI GAATG_Cn' Cuts at: 3055 3773 3866 3055 Size: 718 93 5451 Fragments arranged by size: 5451 718 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2068 2189 2297 2511 3581 4323 Size: 399 222 56 121 108 214 1070 742 Cuts at: 4323 4408 5479 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 1070 742 399 222 214 121 108 85 56 BspEI T'CCGG_A Cuts at: 5925 5925 Size: 6262 BspHI T'CATG_A Cuts at: 4151 5881 4151 Size: 1730 4532 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2294 2653 3617 844 Size: 1450 359 964 3489 Fragments arranged by size: 3489 1450 964 359 BsrBI CCG'CTC Cuts at: 1394 1823 2362 4149 1394 Size: 429 539 1787 3507 Fragments arranged by size: 3507 1787 539 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2719 2908 4757 4931 65 Size: 1183 1471 189 1849 174 1396 Fragments arranged by size: 1849 1471 1396 1183 189 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2309 2345 2664 4906 5093 1700 Size: 110 499 36 319 2242 187 2869 Fragments arranged by size: 2869 2242 499 319 187 110 36 BsrGI T'GTAC_A Cuts at: 96 2774 3291 96 Size: 2678 517 3067 Fragments arranged by size: 3067 2678 517 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6262 BssSI C'ACGA_G Cuts at: 4322 5334 4322 Size: 1012 5250 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6262 BstBI TT'CG_AA Cuts at: 3499 5066 3499 Size: 1567 4695 BstEII G'GTnAC_C Cuts at: 1184 2890 1184 Size: 1706 4556 BstXI CCAn_nnnn'nTGG Cuts at: 2789 3181 2789 Size: 392 5870 BstYI r'GATC_y Cuts at: 1972 2440 2692 3592 3959 4344 4361 5813 5899 Size: 468 252 900 367 385 17 1452 86 Cuts at: 5899 5911 6257 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 900 468 385 367 346 252 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2409 3258 2409 Size: 849 5413 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5710 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5523 Fragments arranged by size: 5523 683 56 BtsI GCAGTG_nn' Cuts at: 2189 2548 3778 4572 4592 2189 Size: 359 1230 794 20 3859 Fragments arranged by size: 3859 1230 794 359 20 ClaI AT'CG_AT Cuts at: 1554 3375 1554 Size: 1821 4441 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2140 2175 529 Size: 35 1576 35 4616 Fragments arranged by size: 4616 1576 35 35 DraI TTT'AAA Cuts at: 3566 3566 Size: 6262 DraIII CAC_nnn'GTG Cuts at: 2898 2898 Size: 6262 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5269 809 Size: 507 3953 1802 Fragments arranged by size: 3953 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2148 2261 3709 Size: 54 1425 50 275 18 318 113 1448 Cuts at: 3709 4598 6227 8 Size: 889 1629 43 Fragments arranged by size: 1629 1448 1425 889 318 275 113 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3709 1537 Size: 275 1897 4090 Fragments arranged by size: 4090 1897 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2447 4192 1353 Size: 1094 1745 3423 Fragments arranged by size: 3423 1745 1094 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4849 5223 5369 387 Size: 1064 138 3260 374 146 1280 Fragments arranged by size: 3260 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2236 2681 2827 2981 3022 3300 3387 Size: 393 604 445 146 154 41 278 87 Cuts at: 3387 4325 4922 5709 5977 1239 Size: 938 597 787 268 1524 Fragments arranged by size: 1524 938 787 604 597 445 393 278 268 154 146 87 41 EcoO109I rG'GnC_Cy Cuts at: 2211 2642 2211 Size: 431 5831 EcoRI G'AATT_C Cuts at: 3571 3949 3571 Size: 378 5884 FspI TGC'GCA Cuts at: 4768 4768 Size: 6262 HaeII r_GCGC'y Cuts at: 1678 2436 5409 1678 Size: 758 2973 2531 Fragments arranged by size: 2973 2531 758 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2917 2949 2975 3007 4903 Size: 32 135 32 1345 32 26 32 1896 Cuts at: 4903 4935 4977 5009 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1896 1345 135 42 32 32 32 32 32 32 26 HincII GTy'rAC Cuts at: 669 1657 1938 3606 3852 669 Size: 988 281 1668 246 3079 Fragments arranged by size: 3079 1668 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6262 HpaI GTT'AAC Cuts at: 3852 3852 Size: 6262 KasI G'GCGC_C Cuts at: 2432 2432 Size: 6262 KpnI G_GTAC'C Cuts at: 2671 3587 2671 Size: 916 5346 MfeI C'AATT_G Cuts at: 3861 3861 Size: 6262 MluI A'CGCG_T Cuts at: 6138 6138 Size: 6262 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2807 2837 5376 5560 1305 Size: 1502 30 2539 184 2007 Fragments arranged by size: 2539 2007 1502 184 30 MscI TGG'CCA Cuts at: 10 64 1489 1832 2263 10 Size: 54 1425 343 431 4009 Fragments arranged by size: 4009 1425 431 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2096 2192 3179 4220 4579 4738 518 Size: 648 930 96 987 1041 359 159 2042 Fragments arranged by size: 2042 1041 987 930 648 359 159 96 MspA1I CmG'CkG Cuts at: 1606 2785 2847 3678 4355 5503 5748 1606 Size: 1179 62 831 677 1148 245 2120 Fragments arranged by size: 2120 1179 1148 831 677 245 62 NaeI GCC'GGC Cuts at: 2311 2311 Size: 6262 NarI GG'CG_CC Cuts at: 2433 2433 Size: 6262 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5710 NdeI CA'TA_TG Cuts at: 387 387 Size: 6262 NgoMIV G'CCGG_C Cuts at: 2309 2309 Size: 6262 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6262 NotI GC'GGCC_GC Cuts at: 3709 3709 Size: 6262 NruI TCG'CGA Cuts at: 2447 2447 Size: 6262 NspI r_CATG'y Cuts at: 1132 1229 3620 5165 1132 Size: 97 2391 1545 2229 Fragments arranged by size: 2391 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2068 1348 Size: 720 5542 PciI A'CATG_T Cuts at: 5161 5161 Size: 6262 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6262 PmeI GTTT'AAAC Cuts at: 3566 3566 Size: 6262 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4331 4363 5844 5876 4331 Size: 32 1481 32 4717 Fragments arranged by size: 4717 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2642 2642 Size: 6262 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6262 PsiI TTA'TAA Cuts at: 3832 3832 Size: 6262 PspOMI G'GGCC_C Cuts at: 2507 2507 Size: 6262 PstI C_TGCA'G Cuts at: 830 2543 3247 3614 830 Size: 1713 704 367 3478 Fragments arranged by size: 3478 1713 704 367 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6262 PvuII CAG'CTG Cuts at: 2785 2847 2785 Size: 62 6200 SalI G'TCGA_C Cuts at: 1936 3604 1936 Size: 1668 4594 SbfI CC_TGCA'GG Cuts at: 3614 3614 Size: 6262 ScaI AGT'ACT Cuts at: 4510 6222 4510 Size: 1712 4550 SexAI A'CCwGG_T Cuts at: 2886 2886 Size: 6262 SfcI C'TryA_G Cuts at: 826 1045 2539 2614 3243 3361 3610 4745 5426 Size: 219 1494 75 629 118 249 1135 681 Cuts at: 5426 5617 826 Size: 191 1471 Fragments arranged by size: 1494 1471 1135 681 629 249 219 191 118 75 SfoI GGC'GCC Cuts at: 2434 2434 Size: 6262 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6262 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6262 SmaI CCC'GGG Cuts at: 1872 3589 1872 Size: 1717 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2401 2833 3093 4366 5267 5529 Size: 197 926 458 432 260 1273 901 262 Cuts at: 5529 820 Size: 1553 Fragments arranged by size: 1553 1273 926 901 458 432 262 260 197 SnaBI TAC'GTA Cuts at: 493 4053 493 Size: 3560 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6262 SphI G_CATG'C Cuts at: 1132 3620 1132 Size: 2488 3774 SspI AAT'ATT Cuts at: 5 52 4186 5 Size: 47 4134 2081 Fragments arranged by size: 4134 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6262 StyI C'CwwG_G Cuts at: 513 1065 3701 513 Size: 552 2636 3074 Fragments arranged by size: 3074 2636 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2126 4289 4306 4459 4644 5089 325 Size: 1255 546 2163 17 153 185 445 1498 Fragments arranged by size: 2163 1498 1255 546 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2458 2774 3291 Size: 274 80 33 51 1443 481 316 517 Cuts at: 3291 4508 6220 96 Size: 1217 1712 138 Fragments arranged by size: 1712 1443 1217 517 481 316 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2749 2781 3429 3461 6222 6254 2749 Size: 32 648 32 2761 32 2757 Fragments arranged by size: 2761 2757 648 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6262 XbaI T'CTAG_A Cuts at: 3598 3598 Size: 6262 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2705 2705 Size: 6262 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6262 XmaI C'CCGG_G Cuts at: 1870 3587 1870 Size: 1717 4545 XmnI GAAnn'nnTTC Cuts at: 4391 4391 Size: 6262 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4070 276 Size: 53 83 186 936 2536 2468 Fragments arranged by size: 2536 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI