Restriction map for
pFN21AE1373
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BanI BbeI
BbsI BcgI BciVI BfaI BfrI
BglI BglII BlnI BpuAI BsaBI
BsaHI BseAI BsiWI BsmI BspDI
BspEI BspHI BspLU11I BsrDI Bst1107I
BstAPI BstBI BstZ17I ClaI CpoI
DraI DraIII DrdI Eam1105I EarI
Eco47III EcoNI EcoRV EcoT22I FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MseI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI TaqI Tru9I Tth111I TthHB8I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AcsI 1141 GAATTC [GA]^AATT[CT]
AlwNI 104 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1141 GAATTC [GA]^AATT[CT]
AspHI 1246 GAGCTC G[AT]GC[AT]^C
BamHI 215 GGATCC G^GATCC
BbrPI 58 CACGTG CAC^GTG
BbvCI 1275 CCTCAGC CCTCAGC(-5/-2)
BsaAI 58 CACGTG [CT]AC^GT[GA]
BsaWI 892 TCCGGT [AT]^CCGG[AT]
BseRI 909 CTCCTC GAGGAG(10/8)
BsiEI 991 CGGCCG CG[GA][CT]^CG
BsiHKAI 1246 GAGCTC G[AT]GC[AT]^C
BsmBI 160 GAGACG CGTCTC(1/5)
Bsp1407I 770 TGTACA T^GTACA
BsrBI 864 CCGCTC CCGCTC(-3/-3)
BsrGI 770 TGTACA T^GTACA
BssHII 606 GCGCGC G^CGCGC
BssSI 1249 CTCGTG CACGAG(-5/-1)
BstEII 402 GGTGACC G^GT[ACGT]ACC
BstPI 402 GGTGACC G^GT[ACGT]ACC
EagI 991 CGGCCG C^GGCCG
EclXI 991 CGGCCG C^GGCCG
Eco52I 991 CGGCCG C^GGCCG
EcoO65I 402 GGTGACC G^GT[ACGT]ACC
EcoRI 1141 GAATTC G^AATTC
PmaCI 58 CACGTG CAC^GTG
PmlI 58 CACGTG CAC^GTG
SacI 1246 GAGCTC GAGCT^C
SmaI 533 CCCGGG CCC^GGG
SspBI 770 TGTACA T^GTACA
StuI 315 AGGCCT AGG^CCT
TfiI 1090 GATTC G^A[AT]TC
Tsp509I 1142 AATT ^AATT
XmaCI 533 CCCGGG C^CCGGG
XmaI 533 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AvaI 31 CTCGGG C^[CT]CG[GA]G
533 CCCGGG
BclI 113 TGATCA T^GATCA
950 TGATCA
BlpI 36 GCTGAGC GC^T[ACGT]AGC
97 GCTGAGC
Bpu1102I 36 GCTGAGC GC^T[ACGT]AGC
97 GCTGAGC
BsgI 61 GTGCAG GTGCAG(16/14)
688 GTGCAG
BsoBI 31 CTCGGG C^[CT]CG[GA]G
533 CCCGGG
BspMI 1217 ACCTGC ACCTGC(4/8)
572 GCAGGT
BsrFI 748 GCCGGC [GA]^CCGG[CT]
809 GCCGGC
BstYI 215 GGATCC [GA]^GATC[CT]
962 AGATCC
CelII 36 GCTGAGC GC^T[ACGT]AGC
97 GCTGAGC
Cfr10I 748 GCCGGC [GA]^CCGG[CT]
809 GCCGGC
DsaI 522 CCGTGG C^C[GA][CT]GG
548 CCGTGG
EaeI 807 TGGCCG [CT]^GGCC[GA]
991 CGGCCG
FauI 1095 CCCGC CCCGC(4/6)
1174 GCGGG
FbaI 113 TGATCA T^GATCA
950 TGATCA
HaeII 800 AGCGCC [GA]GCGC^[CT]
1115 AGCGCC
HgaI 162 GACGC GACGC(5/10)
1101 GCGTC
MflI 215 GGATCC [GA]^GATC[CT]
962 AGATCC
NaeI 748 GCCGGC GCC^GGC
809 GCCGGC
NgoMIV 748 GCCGGC G^CCGGC
809 GCCGGC
NspI 273 ACATGC [GA]CATG^[CT]
886 ACATGC
PflMI 86 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
260 CCATCACCTGG
PvuII 1085 CAGCTG CAG^CTG
1265 CAGCTG
SfaNI 745 GATGC GCATC(5/9)
1053 GATGC
Van91I 86 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
260 CCATCACCTGG
XhoII 215 GGATCC [GA]^GATC[CT]
962 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AocI ApaI AvaII BanII BbvI
BcnI BmyI BpmI BsaI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstXI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII Eco81I EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfcI StyI
TseI Tsp45I TspRI XcmI