(Circular) (Six-base) MAPSORT of: pFN21AE1373.seq Check: 3815 from: 1 to: 6022 pFN21AE1373 6022 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2568 2568 Size: 6022 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3832 278 Size: 53 83 186 936 2296 2468 Fragments arranged by size: 2468 2296 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3365 1937 Size: 1428 4594 Acc65I G'GTAC_C Cuts at: 3343 3343 Size: 6022 AclI AA'CG_TT Cuts at: 1563 4149 4522 1563 Size: 2586 373 3063 Fragments arranged by size: 3063 2586 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4085 5469 5737 1632 Size: 2453 1384 268 1917 Fragments arranged by size: 2453 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5825 AflIII A'CryG_T Cuts at: 1203 4921 5898 1203 Size: 3718 977 1327 Fragments arranged by size: 3718 1327 977 AgeI A'CCGG_T Cuts at: 4853 4853 Size: 6022 AhdI GACnn_n'nnGTC Cuts at: 4751 4751 Size: 6022 AleI CACnn'nnGTG Cuts at: 1166 3237 1166 Size: 2071 3951 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6022 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2501 2533 2501 Size: 32 5990 AlwNI CAG_nnn'CTG Cuts at: 2114 3765 5337 2114 Size: 1651 1572 2799 Fragments arranged by size: 2799 1651 1572 ApaI G_GGCC'C Cuts at: 2079 2561 2861 3005 2079 Size: 482 300 144 5096 Fragments arranged by size: 5096 482 300 144 ApaLI G'TGCA_C Cuts at: 4079 5235 4079 Size: 1156 4866 ApoI r'AATT_y Cuts at: 1493 3146 3331 3557 3709 5818 1493 Size: 1653 185 226 152 2109 1697 Fragments arranged by size: 2109 1697 1653 226 185 152 AseI AT'TA_AT Cuts at: 160 1032 4576 160 Size: 872 3544 1606 Fragments arranged by size: 3544 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6022 AvaI C'yCGr_G Cuts at: 1870 1943 2036 2538 3347 1870 Size: 73 93 502 809 4545 Fragments arranged by size: 4545 809 502 93 73 BamHI G'GATC_C Cuts at: 2220 3352 3719 2220 Size: 1132 367 4523 Fragments arranged by size: 4523 1132 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3343 4798 618 Size: 325 217 627 1556 1455 1842 Fragments arranged by size: 1842 1556 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2079 2561 2570 2655 2835 2861 3005 3255 Size: 688 482 9 85 180 26 144 250 Cuts at: 3255 3341 1391 Size: 86 4072 Fragments arranged by size: 4072 688 482 250 180 144 86 85 26 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5051 BbvCI CC'TCA_GC Cuts at: 3281 3281 Size: 6022 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4213 4247 1903 Size: 34 2276 34 3678 Fragments arranged by size: 3678 2276 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3914 5124 3914 Size: 1210 4812 BclI T'GATC_A Cuts at: 1548 2118 2955 1548 Size: 570 837 4615 Fragments arranged by size: 4615 837 570 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4633 136 Size: 107 122 71 631 605 2961 1525 Fragments arranged by size: 2961 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6017 6017 Size: 6022 BlpI GC'TnA_GC Cuts at: 2042 2103 3439 2042 Size: 61 1336 4625 Fragments arranged by size: 4625 1336 61 Bme1580I G_kGCm'C Cuts at: 1790 2079 2561 2861 3005 4083 5239 1790 Size: 289 482 300 144 1078 1156 2573 Fragments arranged by size: 2573 1156 1078 482 300 289 144 BmgBI CAC'GTC Cuts at: 1318 2792 1318 Size: 1474 4548 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2631 3231 3753 Size: 416 53 83 399 24 1214 600 522 Cuts at: 3753 4711 442 Size: 958 1753 Fragments arranged by size: 1753 1214 958 600 522 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6022 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2128 2128 Size: 6022 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2183 2366 2786 3269 4682 1239 Size: 944 183 420 483 1413 2579 Fragments arranged by size: 2579 1413 944 483 420 183 Bpu10I CC'TnA_GC Cuts at: 3281 3281 Size: 6022 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4147 5012 5310 4147 Size: 865 298 4859 Fragments arranged by size: 4859 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2634 3265 3269 4685 882 Size: 617 1135 631 4 1416 2219 Fragments arranged by size: 2219 1416 1135 631 617 4 BsaAI yAC'GTr Cuts at: 493 1204 2065 3813 493 Size: 711 861 1748 2702 Fragments arranged by size: 2702 1748 861 711 BsaBI GATnn'nnATC Cuts at: 6016 6016 Size: 6022 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3829 4211 4846 275 Size: 53 83 186 936 2296 382 635 1451 Fragments arranged by size: 2296 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2897 4455 4853 5127 5274 5685 2897 Size: 1558 398 274 147 411 3234 Fragments arranged by size: 3234 1558 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2409 2439 2879 2897 2900 Size: 30 165 30 1006 30 440 18 3 Cuts at: 2900 2909 2927 2930 3012 3042 1178 Size: 9 18 3 82 30 4158 Fragments arranged by size: 4158 1006 440 165 82 30 30 30 30 18 18 9 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2905 1186 Size: 1719 4303 BseYI C'CCAG_C Cuts at: 2396 2828 2933 5225 5721 2396 Size: 432 105 2292 496 2697 Fragments arranged by size: 2697 2292 496 432 105 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2087 2714 1163 Size: 573 351 627 4471 Fragments arranged by size: 4471 627 573 351 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2999 3472 4233 5261 1540 Size: 275 186 998 473 761 1028 2301 Fragments arranged by size: 2301 1028 998 761 473 275 186 BsiHKAI G_wGCw'C Cuts at: 3255 4083 4168 5239 3255 Size: 828 85 1071 4038 Fragments arranged by size: 4038 1071 828 85 BsmI GAATG_Cn' Cuts at: 3533 3626 3533 Size: 93 5929 BsmBI CGTCTCn'nnnn_ Cuts at: 2159 2159 Size: 6022 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2561 2570 2655 2835 2861 3005 Size: 399 289 482 9 85 180 26 144 Cuts at: 3005 3255 3341 4083 4168 5239 1391 Size: 250 86 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 482 399 289 250 180 144 86 85 85 26 9 BspEI T'CCGG_A Cuts at: 5685 5685 Size: 6022 BspHI T'CATG_A Cuts at: 3911 5641 3911 Size: 1730 4292 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2568 3231 3377 844 Size: 1724 663 146 3489 Fragments arranged by size: 3489 1724 663 146 BsrBI CCG'CTC Cuts at: 1394 1823 2871 3909 1394 Size: 429 1048 1038 3507 Fragments arranged by size: 3507 1048 1038 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4517 4691 65 Size: 1183 3269 174 1396 Fragments arranged by size: 3269 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2753 2814 4666 4853 1700 Size: 110 943 61 1852 187 2869 Fragments arranged by size: 2869 1852 943 187 110 61 BsrGI T'GTAC_A Cuts at: 96 2775 96 Size: 2679 3343 BssHII G'CGCG_C Cuts at: 1925 2611 1925 Size: 686 5336 BssSI C'ACGA_G Cuts at: 3254 4082 5094 3254 Size: 828 1012 4182 Fragments arranged by size: 4182 1012 828 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6022 BstBI TT'CG_AA Cuts at: 4826 4826 Size: 6022 BstEII G'GTnAC_C Cuts at: 1184 2407 1184 Size: 1223 4799 BstXI CCAn_nnnn'nTGG Cuts at: 2404 2528 2810 2404 Size: 124 282 5616 Fragments arranged by size: 5616 282 124 BstYI r'GATC_y Cuts at: 1972 2220 2967 3352 3719 4104 4121 5573 5659 Size: 248 747 385 367 385 17 1452 86 Cuts at: 5659 5671 6017 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 747 385 385 367 346 248 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2144 2413 3016 2144 Size: 269 603 5150 Fragments arranged by size: 5150 603 269 BtgI C'CryG_G Cuts at: 513 1065 2527 2553 513 Size: 552 1462 26 3982 Fragments arranged by size: 3982 1462 552 26 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5283 Fragments arranged by size: 5283 683 56 BtsI GCAGTG_nn' Cuts at: 2341 3538 4332 4352 2341 Size: 1197 794 20 4011 Fragments arranged by size: 4011 1197 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6022 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5987 DraI TTT'AAA Cuts at: 3326 3326 Size: 6022 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5029 809 Size: 507 3713 1802 Fragments arranged by size: 3713 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2812 2996 3469 Size: 54 1425 50 275 18 982 184 473 Cuts at: 3469 4358 5987 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 982 889 473 275 184 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2996 3469 1537 Size: 275 1184 473 4090 Fragments arranged by size: 4090 1184 473 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3952 1353 Size: 2599 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4609 4983 5129 387 Size: 1064 138 3020 374 146 1280 Fragments arranged by size: 3020 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3253 3253 Size: 6022 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2183 2366 2786 3269 4085 4682 5469 Size: 393 551 183 420 483 816 597 787 Cuts at: 5469 5737 1239 Size: 268 1524 Fragments arranged by size: 1524 816 787 597 551 483 420 393 268 183 EcoO109I rG'GnC_Cy Cuts at: 2058 2075 2076 2558 2702 2858 3002 3183 2058 Size: 17 1 482 144 156 144 181 4897 Fragments arranged by size: 4897 482 181 156 144 144 17 1 EcoRI G'AATT_C Cuts at: 3146 3331 3709 3146 Size: 185 378 5459 Fragments arranged by size: 5459 378 185 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2749 2749 Size: 6022 FspI TGC'GCA Cuts at: 4528 4528 Size: 6022 HaeII r_GCGC'y Cuts at: 1678 2809 3124 5169 1678 Size: 1131 315 2045 2531 Fragments arranged by size: 2531 2045 1131 315 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2557 2589 2970 3002 4663 Size: 32 135 32 985 32 381 32 1661 Cuts at: 4663 4695 4737 4769 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1661 985 381 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3366 3612 669 Size: 988 281 1428 246 3079 Fragments arranged by size: 3079 1428 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6022 HpaI GTT'AAC Cuts at: 3612 3612 Size: 6022 KpnI G_GTAC'C Cuts at: 3347 3347 Size: 6022 MfeI C'AATT_G Cuts at: 3621 3621 Size: 6022 MluI A'CGCG_T Cuts at: 5898 5898 Size: 6022 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3237 3327 5136 5320 1305 Size: 1932 90 1809 184 2007 Fragments arranged by size: 2007 1932 1809 184 90 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4200 Fragments arranged by size: 4200 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2520 2526 3237 3980 4339 4498 518 Size: 648 1354 6 711 743 359 159 2042 Fragments arranged by size: 2042 1354 743 711 648 359 159 6 MspA1I CmG'CkG Cuts at: 1606 3092 3103 3272 3438 4115 5263 5508 1606 Size: 1486 11 169 166 677 1148 245 2120 Fragments arranged by size: 2120 1486 1148 677 245 169 166 11 NaeI GCC'GGC Cuts at: 2755 2816 2755 Size: 61 5961 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5470 NdeI CA'TA_TG Cuts at: 387 387 Size: 6022 NgoMIV G'CCGG_C Cuts at: 2753 2814 2753 Size: 61 5961 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6022 NotI GC'GGCC_GC Cuts at: 3469 3469 Size: 6022 NspI r_CATG'y Cuts at: 1132 1229 2282 2895 3380 4925 1132 Size: 97 1053 613 485 1545 2229 Fragments arranged by size: 2229 1545 1053 613 485 97 PasI CC'CwG_GG Cuts at: 1348 2717 2854 1348 Size: 1369 137 4516 Fragments arranged by size: 4516 1369 137 PciI A'CATG_T Cuts at: 4921 4921 Size: 6022 PflMI CCAn_nnn'nTGG Cuts at: 1108 2097 2271 1108 Size: 989 174 4859 Fragments arranged by size: 4859 989 174 PmeI GTTT'AAAC Cuts at: 3326 3326 Size: 6022 PmlI CAC'GTG Cuts at: 2065 2065 Size: 6022 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4091 4123 5604 5636 4091 Size: 32 1481 32 4477 Fragments arranged by size: 4477 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2058 2702 3183 2058 Size: 644 481 4897 Fragments arranged by size: 4897 644 481 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6022 PsiI TTA'TAA Cuts at: 3592 3592 Size: 6022 PspOMI G'GGCC_C Cuts at: 2075 2557 2857 3001 2075 Size: 482 300 144 5096 Fragments arranged by size: 5096 482 300 144 PstI C_TGCA'G Cuts at: 830 3374 830 Size: 2544 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6022 PvuII CAG'CTG Cuts at: 3092 3272 3092 Size: 180 5842 SacI G_AGCT'C Cuts at: 3255 3255 Size: 6022 SalI G'TCGA_C Cuts at: 1936 3364 1936 Size: 1428 4594 SbfI CC_TGCA'GG Cuts at: 3374 3374 Size: 6022 ScaI AGT'ACT Cuts at: 4270 5982 4270 Size: 1712 4310 SfcI C'TryA_G Cuts at: 826 1045 2316 2368 3187 3370 4505 5186 5377 Size: 219 1271 52 819 183 1135 681 191 Cuts at: 5377 826 Size: 1471 Fragments arranged by size: 1471 1271 1135 819 681 219 191 183 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6022 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6022 SmaI CCC'GGG Cuts at: 1872 2540 3349 1872 Size: 668 809 4545 Fragments arranged by size: 4545 809 668 SmlI C'TyrA_G Cuts at: 820 1017 1943 4126 5027 5289 820 Size: 197 926 2183 901 262 1553 Fragments arranged by size: 2183 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3813 493 Size: 3320 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6022 SphI G_CATG'C Cuts at: 1132 3380 1132 Size: 2248 3774 SspI AAT'ATT Cuts at: 5 52 3946 5 Size: 47 3894 2081 Fragments arranged by size: 3894 2081 47 StuI AGG'CCT Cuts at: 1517 2322 1517 Size: 805 5217 StyI C'CwwG_G Cuts at: 513 1065 2125 3078 3309 3461 513 Size: 552 1060 953 231 152 3074 Fragments arranged by size: 3074 1060 953 552 231 152 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2138 2511 4049 4066 4219 4404 4849 Size: 1255 558 373 1538 17 153 185 445 Cuts at: 4849 325 Size: 1498 Fragments arranged by size: 1538 1498 1255 558 445 373 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2775 2886 4268 Size: 274 80 33 51 1443 798 111 1382 Cuts at: 4268 5980 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1382 798 274 138 111 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2409 2441 3012 3044 5982 6014 2409 Size: 32 571 32 2938 32 2417 Fragments arranged by size: 2938 2417 571 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6022 XbaI T'CTAG_A Cuts at: 3358 3358 Size: 6022 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2551 2577 2644 2851 2941 3244 2551 Size: 26 67 207 90 303 5329 Fragments arranged by size: 5329 303 207 90 67 26 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6022 XmaI C'CCGG_G Cuts at: 1870 2538 3347 1870 Size: 668 809 4545 Fragments arranged by size: 4545 809 668 XmnI GAAnn'nnTTC Cuts at: 4151 4151 Size: 6022 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3830 276 Size: 53 83 186 936 2296 2468 Fragments arranged by size: 2468 2296 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI