(Linear) (Six-base) MAPSORT of: KIEE4553.seq Check: 6616 from: 1 to: 1995 KIEE4553.orf With 182 enzymes: SgfI * March 22, 2012 18:21 .. AclI AA'CG_TT Cuts at: 0 774 1995 Size: 774 1221 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 35 1672 1804 1866 1966 1995 Size: 35 1637 132 62 100 29 Fragments arranged by size: 1637 132 100 62 35 29 AfeI AGC'GCT Cuts at: 0 724 1981 1995 Size: 724 1257 14 Fragments arranged by size: 1257 724 14 AflII C'TTAA_G Cuts at: 0 1453 1995 Size: 1453 542 AflIII A'CryG_T Cuts at: 0 1905 1995 Size: 1905 90 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1157 1995 Size: 1157 838 AlwNI CAG_nnn'CTG Cuts at: 0 73 391 1261 1995 Size: 73 318 870 734 Fragments arranged by size: 870 734 318 73 ApaI G_GGCC'C Cuts at: 0 507 1374 1995 Size: 507 867 621 Fragments arranged by size: 867 621 507 AvaI C'yCGr_G Cuts at: 0 1205 1802 1995 Size: 1205 597 193 Fragments arranged by size: 1205 597 193 BanI G'GyrC_C Cuts at: 0 484 1763 1987 1995 Size: 484 1279 224 8 Fragments arranged by size: 1279 484 224 8 BanII G_rGCy'C Cuts at: 0 450 507 1206 1237 1244 1374 1871 1995 Size: 450 57 699 31 7 130 497 124 Fragments arranged by size: 699 497 450 130 124 57 31 7 BbeI G_GCGC'C Cuts at: 0 1767 1991 1995 Size: 1767 224 4 Fragments arranged by size: 1767 224 4 BbsI GAAGACnn'nnnn_ Cuts at: 0 403 602 1423 1810 1995 Size: 403 199 821 387 185 Fragments arranged by size: 821 403 387 199 185 BbvCI CC'TCA_GC Cuts at: 0 1330 1502 1715 1995 Size: 1330 172 213 280 Fragments arranged by size: 1330 280 213 172 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 581 615 1995 Size: 581 34 1380 Fragments arranged by size: 1380 581 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1027 1995 Size: 1027 968 BclI T'GATC_A Cuts at: 0 1103 1995 Size: 1103 892 BglI GCCn_nnn'nGGC Cuts at: 0 1498 1995 Size: 1498 497 BglII A'GATC_T Cuts at: 0 533 1806 1995 Size: 533 1273 189 Fragments arranged by size: 1273 533 189 BlpI GC'TnA_GC Cuts at: 0 88 1005 1294 1797 1995 Size: 88 917 289 503 198 Fragments arranged by size: 917 503 289 198 88 Bme1580I G_kGCm'C Cuts at: 0 507 1374 1995 Size: 507 867 621 Fragments arranged by size: 867 621 507 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 311 1995 Size: 311 1684 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 104 255 1238 1339 1495 1699 1995 Size: 104 151 983 101 156 204 296 Fragments arranged by size: 983 296 204 156 151 104 101 Bpu10I CC'TnA_GC Cuts at: 0 1238 1330 1502 1715 1995 Size: 1238 92 172 213 280 Fragments arranged by size: 1238 280 213 172 92 BsaI GGTCTCn'nnnn_ Cuts at: 0 572 631 1969 1995 Size: 572 59 1338 26 Fragments arranged by size: 1338 572 59 26 BsaBI GATnn'nnATC Cuts at: 0 1645 1995 Size: 1645 350 BsaHI Gr'CG_yC Cuts at: 0 1764 1988 1995 Size: 1764 224 7 Fragments arranged by size: 1764 224 7 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 569 599 709 739 1051 1081 1995 Size: 569 30 110 30 312 30 914 Fragments arranged by size: 914 569 312 110 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 303 493 717 1237 1318 1720 1995 Size: 303 190 224 520 81 402 275 Fragments arranged by size: 520 402 303 275 224 190 81 BseYI C'CCAG_C Cuts at: 0 391 411 826 1724 1844 1995 Size: 391 20 415 898 120 151 Fragments arranged by size: 898 415 391 151 120 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 343 1995 Size: 343 1652 BsiHKAI G_wGCw'C Cuts at: 0 749 1871 1995 Size: 749 1122 124 Fragments arranged by size: 1122 749 124 BsmI GAATG_Cn' Cuts at: 0 363 1995 Size: 363 1632 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1038 1995 Size: 1038 957 Bsp1286I G_dGCh'C Cuts at: 0 450 507 749 1206 1237 1244 1374 1871 Size: 450 57 242 457 31 7 130 497 Cuts at: 1871 1995 Size: 124 Fragments arranged by size: 497 457 450 242 130 124 57 31 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 304 1995 Size: 304 1691 BsrBI CCG'CTC Cuts at: 0 666 1401 1995 Size: 666 735 594 Fragments arranged by size: 735 666 594 BsrDI GCAATG_nn' Cuts at: 0 1031 1995 Size: 1031 964 BsrFI r'CCGG_y Cuts at: 0 494 842 1972 1995 Size: 494 348 1130 23 Fragments arranged by size: 1130 494 348 23 BssSI C'ACGA_G Cuts at: 0 947 1995 Size: 947 1048 BstAPI GCAn_nnn'nTGC Cuts at: 0 408 675 1995 Size: 408 267 1320 Fragments arranged by size: 1320 408 267 BstXI CCAn_nnnn'nTGG Cuts at: 0 1488 1984 1995 Size: 1488 496 11 Fragments arranged by size: 1488 496 11 BstYI r'GATC_y Cuts at: 0 533 1640 1779 1806 1995 Size: 533 1107 139 27 189 Fragments arranged by size: 1107 533 189 139 27 Bsu36I CC'TnA_GG Cuts at: 0 334 1214 1703 1995 Size: 334 880 489 292 Fragments arranged by size: 880 489 334 292 BtgI C'CryG_G Cuts at: 0 5 442 499 1244 1872 1995 Size: 5 437 57 745 628 123 Fragments arranged by size: 745 628 437 123 57 5 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1534 1995 Size: 1534 461 EaeI y'GGCC_r Cuts at: 0 2 151 496 1310 1974 1995 Size: 2 149 345 814 664 21 Fragments arranged by size: 814 664 345 149 21 2 EarI CTCTTCn'nnn_ Cuts at: 0 1214 1421 1663 1995 Size: 1214 207 242 332 Fragments arranged by size: 1214 332 242 207 EcoICRI GAG'CTC Cuts at: 0 1869 1995 Size: 1869 126 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 35 104 255 1238 1339 1495 1672 1699 Size: 35 69 151 983 101 156 177 27 Cuts at: 1699 1804 1866 1966 1995 Size: 105 62 100 29 Fragments arranged by size: 983 177 156 151 105 101 100 69 62 35 29 27 EcoNI CCTnn'n_nnAGG Cuts at: 0 754 985 1579 1995 Size: 754 231 594 416 Fragments arranged by size: 754 594 416 231 EcoO109I rG'GnC_Cy Cuts at: 0 504 861 939 1150 1210 1370 1371 1434 Size: 504 357 78 211 60 160 1 63 Cuts at: 1434 1896 1995 Size: 462 99 Fragments arranged by size: 504 462 357 211 160 99 78 63 60 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1195 1995 Size: 1195 800 FseI GG_CCGG'CC Cuts at: 0 498 1995 Size: 498 1497 HaeII r_GCGC'y Cuts at: 0 726 1326 1767 1983 1991 1995 Size: 726 600 441 216 8 4 Fragments arranged by size: 726 600 441 216 8 4 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 199 231 609 641 1051 1083 1995 Size: 199 32 378 32 410 32 912 Fragments arranged by size: 912 410 378 199 32 32 32 KasI G'GCGC_C Cuts at: 0 1763 1987 1995 Size: 1763 224 8 Fragments arranged by size: 1763 224 8 MluI A'CGCG_T Cuts at: 0 1905 1995 Size: 1905 90 MspA1I CmG'CkG Cuts at: 0 7 55 172 212 444 768 1995 Size: 7 48 117 40 232 324 1227 Fragments arranged by size: 1227 324 232 117 48 40 7 NaeI GCC'GGC Cuts at: 0 496 1974 1995 Size: 496 1478 21 Fragments arranged by size: 1478 496 21 NarI GG'CG_CC Cuts at: 0 1764 1988 1995 Size: 1764 224 7 Fragments arranged by size: 1764 224 7 NcoI C'CATG_G Cuts at: 0 499 1244 1995 Size: 499 745 751 Fragments arranged by size: 751 745 499 NgoMIV G'CCGG_C Cuts at: 0 494 1972 1995 Size: 494 1478 23 Fragments arranged by size: 1478 494 23 PasI CC'CwG_GG Cuts at: 0 1356 1995 Size: 1356 639 PfoI T'CCnGG_A Cuts at: 0 560 1995 Size: 560 1435 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1780 1812 1995 Size: 1780 32 183 Fragments arranged by size: 1780 183 32 PpuMI rG'GwC_Cy Cuts at: 0 1150 1434 1896 1995 Size: 1150 284 462 99 Fragments arranged by size: 1150 462 284 99 PspOMI G'GGCC_C Cuts at: 0 503 1370 1995 Size: 503 867 625 Fragments arranged by size: 867 625 503 PstI C_TGCA'G Cuts at: 0 467 1136 1995 Size: 467 669 859 Fragments arranged by size: 859 669 467 PvuII CAG'CTG Cuts at: 0 212 1995 Size: 212 1783 SacI G_AGCT'C Cuts at: 0 1871 1995 Size: 1871 124 SacII CC_GC'GG Cuts at: 0 8 445 1995 Size: 8 437 1550 Fragments arranged by size: 1550 437 8 ScaI AGT'ACT Cuts at: 0 294 1995 Size: 294 1701 SexAI A'CCwGG_T Cuts at: 0 581 1995 Size: 581 1414 SfcI C'TryA_G Cuts at: 0 463 698 1132 1285 1600 1732 1995 Size: 463 235 434 153 315 132 263 Fragments arranged by size: 463 434 315 263 235 153 132 SfoI GGC'GCC Cuts at: 0 1765 1989 1995 Size: 1765 224 6 Fragments arranged by size: 1765 224 6 SmlI C'TyrA_G Cuts at: 0 1453 1995 Size: 1453 542 StyI C'CwwG_G Cuts at: 0 499 1094 1145 1244 1995 Size: 499 595 51 99 751 Fragments arranged by size: 751 595 499 99 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 988 1995 Size: 988 1007 TatI w'GTAC_w Cuts at: 0 292 1995 Size: 292 1703 Tth111I GACn'n_nGTC Cuts at: 0 1063 1995 Size: 1063 932 XbaI T'CTAG_A Cuts at: 0 219 1995 Size: 219 1776 Enzymes that do cut: AclI AcuI AfeI AflII AflIII AlfI AlwNI ApaI AvaI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BplI BpmI Bpu10I BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI HaeII Hin4I KasI MluI MspA1I NaeI NarI NcoI NgoMIV PasI PfoI PpiI PpuMI PspOMI PstI PvuII SacI SacII ScaI SexAI SfcI SfoI SmlI StyI TaqII TatI Tth111I XbaI Enzymes that do not cut: AarI AatII AccI Acc65I AgeI AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BfrBI BmgBI BmrI BmtI BpuEI BsaAI BsaWI BsiEI BsiWI BspEI BspHI BsrGI BssHII BstBI BstEII BstZ17I BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI EcoRV FspI FspAI HincII HindIII HpaI KpnI MfeI MmeI MscI MslI NdeI NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI XcmI XhoI XmaI XmnI ZraI