################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MseI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tth111I TthHB8I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 27 enzymes) # ################################### AccI 316 GTAGAC GT^[AC][GT]AC AcsI 57 AAATTT [GA]^AATT[CT] AcyI 319 GACGCC G[GA]^CG[CT]C ApoI 57 AAATTT [GA]^AATT[CT] AspHI 586 GTGCTC G[AT]GC[AT]^C BalI 218 TGGCCA TGG^CCA BbsI 846 GAAGAC GAAGAC(2/6) BpuAI 846 GAAGAC GAAGAC(2/6) BsaHI 319 GACGCC G[GA]^CG[CT]C BsaI 754 GAGACC GGTCTC(1/5) BsiHKAI 586 GTGCTC G[AT]GC[AT]^C BsmFI 206 GGGAC GGGAC(10/14) BspMI 484 ACCTGC ACCTGC(4/8) BssSI 112 CTCGTG CACGAG(-5/-1) Cac8I 810 GCTCGC GC[ACGT]^[ACGT]GC EcoNI 901 CCTACCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG Hin1I 319 GACGCC G[GA]^CG[CT]C MaeII 689 ACGT A^CGT MluNI 218 TGGCCA TGG^CCA MscI 218 TGGCCA TGG^CCA NspI 408 GCATGT [GA]CATG^[CT] PpuMI 1084 AGGACCC [GA]G^G[AT]CC[CT] PvuII 542 CAGCTG CAG^CTG SfaNI 311 GCATC GCATC(5/9) SmlI 966 CTCAAG C^T[CT][GA]AG Tsp509I 58 AATT ^AATT XbaI 456 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### ApaI 38 GGGCCC GGGCC^C 561 GGGCCC AvaII 612 GGACC G^G[AT]CC 1085 GGACC BamHI 51 GGATCC G^GATCC 282 GGATCC BanI 619 GGCACC G^G[CT][GA]CC 979 GGTGCC BanII 38 GGGCCC G[GA]GC[CT]^C 561 GGGCCC BfaI 232 CTAG C^TAG 457 CTAG BpmI 212 CTCCAG CTGGAG(16/14) 650 CTGGAG BsgI 120 CTGCAC GTGCAG(16/14) 73 GTGCAG BstXI 55 CCAAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 551 CCACCTCCCTGG BstYI 51 GGATCC [GA]^GATC[CT] 282 GGATCC DsaI 601 CCACGG C^C[GA][CT]GG 615 CCACGG EaeI 218 TGGCCA [CT]^GGCC[GA] 465 CGGCCA EcoT14I 276 CCTTGG C^C[AT][AT]GG 1052 CCAAGG HgaI 319 GACGC GACGC(5/10) 107 GCGTC MaeI 232 CTAG C^TAG 457 CTAG MflI 51 GGATCC [GA]^GATC[CT] 282 GGATCC MspA1I 4 CCGCTG C[AC]G^C[GT]G 542 CAGCTG StyI 276 CCTTGG C^C[AT][AT]GG 1052 CCAAGG TfiI 266 GATTC G^A[AT]TC 1044 GAATC XcmI 758 CCAGAGCAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 869 CCAGGACAGCAGTGG XhoII 51 GGATCC [GA]^GATC[CT] 282 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AfaI AluI AlwI BbvI BcnI BmyI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI RsaI Sau3AI Sau96I ScrFI TseI Tsp45I TspRI Van91I