(Circular) (Six-base) MAPSORT of: pFN21AE1425.seq Check: 5843 from: 1 to: 5797 pFN21AE1425 5797 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3607 278 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2322 3140 1937 Size: 385 818 4594 Fragments arranged by size: 4594 818 385 Acc65I G'GTAC_C Cuts at: 3118 3118 Size: 5797 AclI AA'CG_TT Cuts at: 1563 3924 4297 1563 Size: 2361 373 3063 Fragments arranged by size: 3063 2361 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3860 5244 5512 1632 Size: 2228 1384 268 1917 Fragments arranged by size: 2228 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5600 AflIII A'CryG_T Cuts at: 1203 4696 5673 1203 Size: 3493 977 1327 Fragments arranged by size: 3493 1327 977 AgeI A'CCGG_T Cuts at: 4628 4628 Size: 5797 AhdI GACnn_n'nnGTC Cuts at: 4526 4526 Size: 5797 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5797 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5797 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2291 2323 2291 Size: 32 5765 AlwNI CAG_nnn'CTG Cuts at: 3540 5112 3540 Size: 1572 4225 ApaI G_GGCC'C Cuts at: 2047 2570 2047 Size: 523 5274 ApaLI G'TGCA_C Cuts at: 3854 5010 3854 Size: 1156 4641 ApoI r'AATT_y Cuts at: 1493 2062 3106 3332 3484 5593 1493 Size: 569 1044 226 152 2109 1697 Fragments arranged by size: 2109 1697 1044 569 226 152 AseI AT'TA_AT Cuts at: 160 1032 4351 160 Size: 872 3319 1606 Fragments arranged by size: 3319 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 AvaI C'yCGr_G Cuts at: 1870 1943 3122 1870 Size: 73 1179 4545 Fragments arranged by size: 4545 1179 73 BamHI G'GATC_C Cuts at: 2056 2287 3127 3494 2056 Size: 231 840 367 4359 Fragments arranged by size: 4359 840 367 231 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2624 2984 3118 4573 618 Size: 325 217 627 837 360 134 1455 1842 Fragments arranged by size: 1842 1455 837 627 360 325 217 134 BanII G_rGCy'C Cuts at: 1391 2047 2570 3116 1391 Size: 656 523 546 4072 Fragments arranged by size: 4072 656 546 523 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2858 928 Size: 971 959 3867 Fragments arranged by size: 3867 971 959 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3988 4022 1903 Size: 34 50 34 1967 34 3678 Fragments arranged by size: 3678 1967 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3689 4899 3689 Size: 1210 4587 BclI T'GATC_A Cuts at: 1548 1548 Size: 5797 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4408 136 Size: 107 122 71 631 605 2736 1525 Fragments arranged by size: 2736 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5792 5792 Size: 5797 BlpI GC'TnA_GC Cuts at: 3214 3214 Size: 5797 Bme1580I G_kGCm'C Cuts at: 1790 2047 2124 2570 3858 5014 1790 Size: 257 77 446 1288 1156 2573 Fragments arranged by size: 2573 1288 1156 446 257 77 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5797 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2162 2286 3528 Size: 416 53 83 399 24 745 124 1242 Cuts at: 3528 4486 442 Size: 958 1753 Fragments arranged by size: 1753 1242 958 745 416 399 124 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5797 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2202 2676 4457 1239 Size: 963 474 1781 2579 Fragments arranged by size: 2579 1781 963 474 Bpu10I CC'TnA_GC Cuts at: 2312 2746 2312 Size: 434 5363 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2956 3922 4787 5085 2956 Size: 966 865 298 3668 Fragments arranged by size: 3668 966 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2753 4460 882 Size: 617 1254 1707 2219 Fragments arranged by size: 2219 1707 1254 617 BsaAI yAC'GTr Cuts at: 493 1204 3588 493 Size: 711 2384 2702 Fragments arranged by size: 2702 2384 711 BsaBI GATnn'nnATC Cuts at: 5791 5791 Size: 5797 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2325 3604 3986 4621 Size: 53 83 186 936 792 1279 382 635 Cuts at: 4621 275 Size: 1451 Fragments arranged by size: 1451 1279 936 792 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4230 4628 4902 5049 5460 4230 Size: 398 274 147 411 4567 Fragments arranged by size: 4567 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2093 2123 2147 2177 2291 Size: 30 165 30 690 30 24 30 114 Cuts at: 2291 2321 2649 2679 2787 2817 1178 Size: 30 328 30 108 30 4158 Fragments arranged by size: 4158 690 328 165 114 108 30 30 30 30 30 30 30 24 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2270 2347 2575 2808 3026 1186 Size: 1084 77 228 233 218 3957 Fragments arranged by size: 3957 1084 233 228 218 77 BseYI C'CCAG_C Cuts at: 2400 5000 5496 2400 Size: 2600 496 2701 Fragments arranged by size: 2701 2600 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2099 2110 1163 Size: 573 363 11 4850 Fragments arranged by size: 4850 573 363 11 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3247 4008 5036 1540 Size: 275 186 1246 761 1028 2301 Fragments arranged by size: 2301 1246 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2595 3858 3943 5014 2595 Size: 1263 85 1071 3378 Fragments arranged by size: 3378 1263 1071 85 BsmI GAATG_Cn' Cuts at: 3308 3401 3308 Size: 93 5704 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2047 2124 2570 2595 3116 3858 3943 Size: 399 257 77 446 25 521 742 85 Cuts at: 3943 5014 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 521 446 399 257 85 77 25 BspEI T'CCGG_A Cuts at: 5460 5460 Size: 5797 BspHI T'CATG_A Cuts at: 3686 5416 3686 Size: 1730 4067 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2498 3152 844 Size: 1654 654 3489 Fragments arranged by size: 3489 1654 654 BsrBI CCG'CTC Cuts at: 1394 1823 3684 1394 Size: 429 1861 3507 Fragments arranged by size: 3507 1861 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4292 4466 65 Size: 1183 3044 174 1396 Fragments arranged by size: 3044 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4441 4628 1700 Size: 110 2631 187 2869 Fragments arranged by size: 2869 2631 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5797 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5797 BssSI C'ACGA_G Cuts at: 2117 3857 4869 2117 Size: 1740 1012 3045 Fragments arranged by size: 3045 1740 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5797 BstBI TT'CG_AA Cuts at: 4601 4601 Size: 5797 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5797 BstXI CCAn_nnnn'nTGG Cuts at: 2067 2563 2067 Size: 496 5301 BstYI r'GATC_y Cuts at: 1972 2056 2287 3127 3494 3879 3896 5348 5434 Size: 84 231 840 367 385 17 1452 86 Cuts at: 5434 5446 5792 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 840 385 367 346 231 86 84 17 12 BtgI C'CryG_G Cuts at: 513 1065 2606 2620 513 Size: 552 1541 14 3690 Fragments arranged by size: 3690 1541 552 14 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5058 Fragments arranged by size: 5058 683 56 BtsI GCAGTG_nn' Cuts at: 2889 3313 4107 4127 2889 Size: 424 794 20 4559 Fragments arranged by size: 4559 794 424 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5797 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5762 DraI TTT'AAA Cuts at: 3101 3101 Size: 5797 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4804 809 Size: 507 3488 1802 Fragments arranged by size: 3488 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2223 2470 3244 Size: 54 1425 50 275 18 393 247 774 Cuts at: 3244 4133 5762 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 774 393 275 247 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3244 1537 Size: 275 1432 4090 Fragments arranged by size: 4090 1432 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3727 1353 Size: 2374 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2264 4384 4758 4904 387 Size: 1064 138 675 2120 374 146 1280 Fragments arranged by size: 2120 1280 1064 675 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2202 2676 3860 4457 5244 5512 1239 Size: 393 570 474 1184 597 787 268 1524 Fragments arranged by size: 1524 1184 787 597 570 474 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2910 2910 Size: 5797 EcoO109I rG'GnC_Cy Cuts at: 2043 2044 2567 3090 2043 Size: 1 523 523 4750 Fragments arranged by size: 4750 523 523 1 EcoRI G'AATT_C Cuts at: 3106 3484 3106 Size: 378 5419 FspI TGC'GCA Cuts at: 4303 4303 Size: 5797 HaeII r_GCGC'y Cuts at: 1678 4944 1678 Size: 3266 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2147 2179 2262 2294 4438 Size: 32 135 32 575 32 83 32 2144 Cuts at: 4438 4470 4512 4544 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2144 575 135 83 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3141 3387 669 Size: 988 281 1203 246 3079 Fragments arranged by size: 3079 1203 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5797 HpaI GTT'AAC Cuts at: 3387 3387 Size: 5797 KpnI G_GTAC'C Cuts at: 3122 3122 Size: 5797 MfeI C'AATT_G Cuts at: 3396 3396 Size: 5797 MluI A'CGCG_T Cuts at: 5673 5673 Size: 5797 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4911 5095 1305 Size: 3606 184 2007 Fragments arranged by size: 3606 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2225 10 Size: 54 1425 343 393 3582 Fragments arranged by size: 3582 1425 393 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3755 4114 4273 518 Size: 648 2589 359 159 2042 Fragments arranged by size: 2589 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2011 2549 3213 3890 5038 5283 1606 Size: 405 538 664 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 664 538 405 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5245 NdeI CA'TA_TG Cuts at: 387 387 Size: 5797 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5797 NotI GC'GGCC_GC Cuts at: 3244 3244 Size: 5797 NspI r_CATG'y Cuts at: 1132 1229 2417 3155 4700 1132 Size: 97 1188 738 1545 2229 Fragments arranged by size: 2229 1545 1188 738 97 PasI CC'CwG_GG Cuts at: 1348 2563 1348 Size: 1215 4582 PciI A'CATG_T Cuts at: 4696 4696 Size: 5797 PflMI CCAn_nnn'nTGG Cuts at: 1108 2174 2723 2741 1108 Size: 1066 549 18 4164 Fragments arranged by size: 4164 1066 549 18 PfoI T'CCnGG_A Cuts at: 2187 2734 2187 Size: 547 5250 PmeI GTTT'AAAC Cuts at: 3101 3101 Size: 5797 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2291 2323 3866 3898 5379 5411 2291 Size: 32 1543 32 1481 32 2677 Fragments arranged by size: 2677 1543 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3090 3090 Size: 5797 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5797 PsiI TTA'TAA Cuts at: 3367 3367 Size: 5797 PspOMI G'GGCC_C Cuts at: 2043 2566 2043 Size: 523 5274 PstI C_TGCA'G Cuts at: 830 3149 830 Size: 2319 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5797 PvuII CAG'CTG Cuts at: 2549 2549 Size: 5797 SalI G'TCGA_C Cuts at: 1936 3139 1936 Size: 1203 4594 SbfI CC_TGCA'GG Cuts at: 3149 3149 Size: 5797 ScaI AGT'ACT Cuts at: 4045 5757 4045 Size: 1712 4085 SfcI C'TryA_G Cuts at: 826 1045 3145 4280 4961 5152 826 Size: 219 2100 1135 681 191 1471 Fragments arranged by size: 2100 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5797 SmaI CCC'GGG Cuts at: 1872 3124 1872 Size: 1252 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2971 3901 4802 5064 820 Size: 197 926 1028 930 901 262 1553 Fragments arranged by size: 1553 1028 930 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3588 493 Size: 3095 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5797 SphI G_CATG'C Cuts at: 1132 3155 1132 Size: 2023 3774 SspI AAT'ATT Cuts at: 5 52 3721 5 Size: 47 3669 2081 Fragments arranged by size: 3669 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5797 StyI C'CwwG_G Cuts at: 513 1065 2281 3057 3236 513 Size: 552 1216 776 179 3074 Fragments arranged by size: 3074 1216 776 552 179 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2915 3824 3841 3994 4179 4624 325 Size: 1255 1335 909 17 153 185 445 1498 Fragments arranged by size: 1498 1335 1255 909 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2248 4043 5755 Size: 274 80 33 51 1443 271 1795 1712 Cuts at: 5755 96 Size: 138 Fragments arranged by size: 1795 1712 1443 274 271 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2093 2125 2649 2681 2689 2721 5757 5789 2093 Size: 32 524 32 8 32 3036 32 2101 Fragments arranged by size: 3036 2101 524 32 32 32 32 8 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5797 XbaI T'CTAG_A Cuts at: 2461 3133 2461 Size: 672 5125 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2770 2881 2770 Size: 111 5686 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5797 XmaI C'CCGG_G Cuts at: 1870 3122 1870 Size: 1252 4545 XmnI GAAnn'nnTTC Cuts at: 3926 3926 Size: 5797 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3605 276 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI