(Circular) (Six-base) MAPSORT of: pFN21AE6704.seq Check: 5011 from: 1 to: 7888 pFN21AE6704 7888 bp With 182 enzymes: SgfI * June 19, 2019 18:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3287 3477 5698 278 Size: 53 83 186 936 1751 190 2221 2468 Fragments arranged by size: 2468 2221 1751 936 190 186 83 53 AccI GT'mk_AC Cuts at: 1937 5231 1937 Size: 3294 4594 Acc65I G'GTAC_C Cuts at: 5209 5209 Size: 7888 AclI AA'CG_TT Cuts at: 1563 6015 6388 1563 Size: 4452 373 3063 Fragments arranged by size: 4452 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2099 2794 3829 3986 4220 4271 4613 5951 Size: 467 695 1035 157 234 51 342 1338 Cuts at: 5951 7335 7603 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1338 1035 695 467 342 268 234 157 51 AfeI AGC'GCT Cuts at: 3971 5151 3971 Size: 1180 6708 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7691 AflIII A'CryG_T Cuts at: 1203 3648 6787 7764 1203 Size: 2445 3139 977 1327 Fragments arranged by size: 3139 2445 1327 977 AgeI A'CCGG_T Cuts at: 2469 6719 2469 Size: 4250 3638 AhdI GACnn_n'nnGTC Cuts at: 6617 6617 Size: 7888 AleI CACnn'nnGTG Cuts at: 1166 3302 1166 Size: 2136 5752 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2693 4385 1184 Size: 1509 1692 4687 Fragments arranged by size: 4687 1692 1509 AlwNI CAG_nnn'CTG Cuts at: 2533 2817 3256 3892 3991 4243 4555 4660 4756 Size: 284 439 636 99 252 312 105 96 Cuts at: 4756 4927 5631 7203 2533 Size: 171 704 1572 3218 Fragments arranged by size: 3218 1572 704 636 439 312 284 252 171 105 99 96 ApaI G_GGCC'C Cuts at: 3352 3352 Size: 7888 ApaLI G'TGCA_C Cuts at: 4132 5945 7101 4132 Size: 1813 1156 4919 Fragments arranged by size: 4919 1813 1156 ApoI r'AATT_y Cuts at: 1493 2411 3560 4315 5197 5423 5575 7684 1493 Size: 918 1149 755 882 226 152 2109 1697 Fragments arranged by size: 2109 1697 1149 918 882 755 226 152 AseI AT'TA_AT Cuts at: 160 1032 6442 160 Size: 872 5410 1606 Fragments arranged by size: 5410 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7888 AvaI C'yCGr_G Cuts at: 1870 1943 2063 2624 2861 4700 5213 1870 Size: 73 120 561 237 1839 513 4545 Fragments arranged by size: 4545 1839 561 513 237 120 73 AvrII C'CTAG_G Cuts at: 2443 5032 2443 Size: 2589 5299 BamHI G'GATC_C Cuts at: 3786 5218 5585 3786 Size: 1432 367 6089 Fragments arranged by size: 6089 1432 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2934 4004 4257 4475 4560 Size: 325 217 627 1147 1070 253 218 85 Cuts at: 4560 5209 6664 618 Size: 649 1455 1842 Fragments arranged by size: 1842 1455 1147 1070 649 627 325 253 218 217 85 BanII G_rGCy'C Cuts at: 1391 2829 2850 2901 3352 3411 3833 4784 5207 Size: 1438 21 51 451 59 422 951 423 Cuts at: 5207 1391 Size: 4072 Fragments arranged by size: 4072 1438 951 451 423 422 59 51 21 BbeI G_GCGC'C Cuts at: 4008 4008 Size: 7888 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2433 3157 3477 4035 928 Size: 971 534 724 320 558 4781 Fragments arranged by size: 4781 971 724 558 534 320 BbvCI CC'TCA_GC Cuts at: 2462 4890 2462 Size: 2428 5460 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5040 5074 6079 6113 1903 Size: 34 50 34 3019 34 1005 34 3678 Fragments arranged by size: 3678 3019 1005 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2287 5780 6990 2287 Size: 3493 1210 3185 Fragments arranged by size: 3493 3185 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7888 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4779 5079 6499 Size: 107 122 71 631 605 3107 300 1420 Cuts at: 6499 136 Size: 1525 Fragments arranged by size: 3107 1525 1420 631 605 300 122 107 71 BglII A'GATC_T Cuts at: 4596 7883 4596 Size: 3287 4601 BlpI GC'TnA_GC Cuts at: 4273 5069 5305 4273 Size: 796 236 6856 Fragments arranged by size: 6856 796 236 Bme1580I G_kGCm'C Cuts at: 1790 3352 4136 5949 7105 1790 Size: 1562 784 1813 1156 2573 Fragments arranged by size: 2573 1813 1562 1156 784 BmgBI CAC'GTC Cuts at: 1318 3625 1318 Size: 2307 5581 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2713 4390 4559 Size: 416 53 83 399 24 1296 1677 169 Cuts at: 4559 4879 5619 6577 442 Size: 320 740 958 1753 Fragments arranged by size: 1753 1677 1296 958 740 416 399 320 169 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2580 1056 Size: 1524 6364 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2238 4306 4651 2238 Size: 2068 345 5475 Fragments arranged by size: 5475 2068 345 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2233 2825 3086 3230 3278 3344 3749 4252 Size: 994 592 261 144 48 66 405 503 Cuts at: 4252 4457 4541 4733 4799 4904 6548 1239 Size: 205 84 192 66 105 1644 2579 Fragments arranged by size: 2579 1644 994 592 503 405 261 205 192 144 105 84 66 66 48 Bpu10I CC'TnA_GC Cuts at: 2462 2823 3006 3893 4676 4890 2462 Size: 361 183 887 783 214 5460 Fragments arranged by size: 5460 887 783 361 214 183 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2885 3568 4867 6013 6878 7176 2885 Size: 683 1299 1146 865 298 3597 Fragments arranged by size: 3597 1299 1146 865 683 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2588 3707 3757 3955 6551 882 Size: 617 1089 1119 50 198 2596 2219 Fragments arranged by size: 2596 2219 1119 1089 617 198 50 BsaAI yAC'GTr Cuts at: 493 1204 3175 4740 5679 493 Size: 711 1971 1565 939 2702 Fragments arranged by size: 2702 1971 1565 939 711 BsaBI GATnn'nnATC Cuts at: 7882 7882 Size: 7888 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3284 3474 4005 4101 Size: 53 83 186 936 1751 190 531 96 Cuts at: 4101 5695 6077 6712 275 Size: 1594 382 635 1451 Fragments arranged by size: 1751 1594 1451 936 635 531 382 190 186 96 83 53 BsaWI w'CCGG_w Cuts at: 2469 3447 6321 6719 6993 7140 7551 2469 Size: 978 2874 398 274 147 411 2806 Fragments arranged by size: 2874 2806 978 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3860 3890 3914 3935 3944 Size: 30 165 30 2457 30 24 21 9 Cuts at: 3944 3965 1178 Size: 21 5101 Fragments arranged by size: 5101 2457 165 30 30 30 24 21 21 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2219 2630 2963 3449 3725 3884 3887 3943 Size: 1033 411 333 486 276 159 3 56 Cuts at: 3943 1186 Size: 5131 Fragments arranged by size: 5131 1033 486 411 333 276 159 56 3 BseYI C'CCAG_C Cuts at: 2116 2175 2828 2943 3225 4690 4777 7091 7587 Size: 59 653 115 282 1465 87 2314 496 Cuts at: 7587 2116 Size: 2417 Fragments arranged by size: 2417 2314 1465 653 496 282 115 87 59 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3977 4091 4420 1163 Size: 573 2241 114 329 4631 Fragments arranged by size: 4631 2241 573 329 114 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5338 6099 7127 1540 Size: 275 186 3337 761 1028 2301 Fragments arranged by size: 3337 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2502 2850 2901 3072 3392 3945 4046 4136 4473 Size: 348 51 171 320 553 101 90 337 Cuts at: 4473 4815 5949 6034 7105 2502 Size: 342 1134 85 1071 3285 Fragments arranged by size: 3285 1134 1071 553 348 342 337 320 171 101 90 85 51 BsmI GAATG_Cn' Cuts at: 2792 5399 5492 2792 Size: 2607 93 5188 Fragments arranged by size: 5188 2607 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3481 3481 Size: 7888 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2502 2829 2850 2901 3072 3352 3392 Size: 399 712 327 21 51 171 280 40 Cuts at: 3392 3411 3833 3945 4046 4136 4473 4784 4815 Size: 19 422 112 101 90 337 311 31 Cuts at: 4815 5207 5949 6034 7105 1391 Size: 392 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 712 422 399 392 337 327 311 280 171 112 101 90 85 51 40 31 21 19 BspEI T'CCGG_A Cuts at: 3447 7551 3447 Size: 4104 3784 BspHI T'CATG_A Cuts at: 5777 7507 5777 Size: 1730 6158 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2506 3248 3327 5243 844 Size: 1662 742 79 1916 3489 Fragments arranged by size: 3489 1916 1662 742 79 BsrBI CCG'CTC Cuts at: 1394 1823 2239 5775 1394 Size: 429 416 3536 3507 Fragments arranged by size: 3536 3507 429 416 BsrDI GCAATG_nn' Cuts at: 65 1248 2440 3924 6383 6557 65 Size: 1183 1192 1484 2459 174 1396 Fragments arranged by size: 2459 1484 1396 1192 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2156 2469 4773 6532 6719 1700 Size: 110 346 313 2304 1759 187 2869 Fragments arranged by size: 2869 2304 1759 346 313 187 110 BsrGI T'GTAC_A Cuts at: 96 3306 96 Size: 3210 4678 BssHII G'CGCG_C Cuts at: 1925 2438 2832 1925 Size: 513 394 6981 Fragments arranged by size: 6981 513 394 BssSI C'ACGA_G Cuts at: 5948 6960 5948 Size: 1012 6876 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7888 BstBI TT'CG_AA Cuts at: 6692 6692 Size: 7888 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7888 BstXI CCAn_nnnn'nTGG Cuts at: 2053 3043 3082 2053 Size: 990 39 6859 Fragments arranged by size: 6859 990 39 BstYI r'GATC_y Cuts at: 1972 2130 2374 3087 3786 3861 4330 4596 5218 Size: 158 244 713 699 75 469 266 622 Cuts at: 5218 5585 5970 5987 7439 7525 7537 7883 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 713 699 622 469 385 367 346 266 244 158 86 75 17 12 Bsu36I CC'TnA_GG Cuts at: 2922 4244 4535 4727 2922 Size: 1322 291 192 6083 Fragments arranged by size: 6083 1322 291 192 BtgI C'CryG_G Cuts at: 513 1065 2562 2640 2781 2961 3036 513 Size: 552 1497 78 141 180 75 5365 Fragments arranged by size: 5365 1497 552 180 141 78 75 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7149 Fragments arranged by size: 7149 683 56 BtsI GCAGTG_nn' Cuts at: 3297 3309 3788 4608 5404 6198 6218 3297 Size: 12 479 820 796 794 20 4967 Fragments arranged by size: 4967 820 796 794 479 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7888 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7853 DraI TTT'AAA Cuts at: 5192 5192 Size: 7888 DraIII CAC_nnn'GTG Cuts at: 2306 2692 3204 2306 Size: 386 512 6990 Fragments arranged by size: 6990 512 386 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5045 6895 809 Size: 507 3729 1850 1802 Fragments arranged by size: 3729 1850 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5335 6224 7853 Size: 54 1425 50 275 18 3505 889 1629 Cuts at: 7853 8 Size: 43 Fragments arranged by size: 3505 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5335 1537 Size: 275 3523 4090 Fragments arranged by size: 4090 3523 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2726 2750 3200 3728 4247 5818 1353 Size: 1373 24 450 528 519 1571 3423 Fragments arranged by size: 3423 1571 1373 528 519 450 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2723 6475 6849 6995 387 Size: 1064 138 1134 3752 374 146 1280 Fragments arranged by size: 3752 1280 1134 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2848 2899 2848 Size: 51 7837 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2099 2233 2794 2825 3086 3230 3278 Size: 393 467 134 561 31 261 144 48 Cuts at: 3278 3344 3749 3829 3986 4220 4252 4271 4457 Size: 66 405 80 157 234 32 19 186 Cuts at: 4457 4541 4613 4733 4799 4904 5951 6548 7335 Size: 84 72 120 66 105 1047 597 787 Cuts at: 7335 7603 1239 Size: 268 1524 Fragments arranged by size: 1524 1047 787 597 561 467 405 393 268 261 234 186 157 144 134 120 105 84 80 72 66 66 48 32 31 19 EcoNI CCTnn'n_nnAGG Cuts at: 3431 3590 3707 3824 3431 Size: 159 117 117 7495 Fragments arranged by size: 7495 159 117 117 EcoO109I rG'GnC_Cy Cuts at: 2254 2281 2927 3348 3349 3442 3889 4285 4381 Size: 27 646 421 1 93 447 396 96 Cuts at: 4381 4696 4723 4873 2254 Size: 315 27 150 5269 Fragments arranged by size: 5269 646 447 421 396 315 150 96 93 27 27 1 EcoRI G'AATT_C Cuts at: 3560 5197 5575 3560 Size: 1637 378 5873 Fragments arranged by size: 5873 1637 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2372 3139 2372 Size: 767 7121 FspI TGC'GCA Cuts at: 6394 6394 Size: 7888 HaeII r_GCGC'y Cuts at: 1678 2623 2869 3973 3984 4008 5153 7035 1678 Size: 945 246 1104 11 24 1145 1882 2531 Fragments arranged by size: 2531 1882 1145 1104 945 246 24 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4414 4446 6529 6561 6603 Size: 32 135 32 2842 32 2083 32 42 Cuts at: 6603 6635 1373 Size: 32 2626 Fragments arranged by size: 2842 2626 2083 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2334 2458 3639 5232 5478 669 Size: 988 281 396 124 1181 1593 246 3079 Fragments arranged by size: 3079 1593 1181 988 396 281 246 124 HindIII A'AGCT_T Cuts at: 748 748 Size: 7888 HpaI GTT'AAC Cuts at: 5478 5478 Size: 7888 KasI G'GCGC_C Cuts at: 4004 4004 Size: 7888 KpnI G_GTAC'C Cuts at: 5213 5213 Size: 7888 MfeI C'AATT_G Cuts at: 5487 5487 Size: 7888 MluI A'CGCG_T Cuts at: 3648 7764 3648 Size: 4116 3772 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4330 4479 5074 7002 7186 1305 Size: 3025 149 595 1928 184 2007 Fragments arranged by size: 3025 2007 1928 595 184 149 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6066 Fragments arranged by size: 6066 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3302 3767 3999 5846 6205 6364 518 Size: 648 2136 465 232 1847 359 159 2042 Fragments arranged by size: 2136 2042 1847 648 465 359 232 159 MspA1I CmG'CkG Cuts at: 1606 2179 2814 3256 3849 4124 4435 5304 5981 Size: 573 635 442 593 275 311 869 677 Cuts at: 5981 7129 7374 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 869 677 635 593 573 442 311 275 245 NaeI GCC'GGC Cuts at: 4775 4775 Size: 7888 NarI GG'CG_CC Cuts at: 4005 4005 Size: 7888 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7336 NdeI CA'TA_TG Cuts at: 387 387 Size: 7888 NgoMIV G'CCGG_C Cuts at: 4773 4773 Size: 7888 NheI G'CTAG_C Cuts at: 1052 2576 1052 Size: 1524 6364 NotI GC'GGCC_GC Cuts at: 5335 5335 Size: 7888 NspI r_CATG'y Cuts at: 1132 1229 2340 2797 3083 3313 5246 6791 1132 Size: 97 1111 457 286 230 1933 1545 2229 Fragments arranged by size: 2229 1933 1545 1111 457 286 230 97 PasI CC'CwG_GG Cuts at: 1348 2136 4177 4984 1348 Size: 788 2041 807 4252 Fragments arranged by size: 4252 2041 807 788 PciI A'CATG_T Cuts at: 6787 6787 Size: 7888 PflMI CCAn_nnn'nTGG Cuts at: 1108 2306 1108 Size: 1198 6690 PfoI T'CCnGG_A Cuts at: 2841 3287 3555 3585 3864 4833 2841 Size: 446 268 30 279 969 5896 Fragments arranged by size: 5896 969 446 279 268 30 PmeI GTTT'AAAC Cuts at: 5192 5192 Size: 7888 PmlI CAC'GTG Cuts at: 4740 4740 Size: 7888 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5957 5989 7470 7502 5957 Size: 32 1481 32 6343 Fragments arranged by size: 6343 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2254 2281 2927 3442 4285 4696 2254 Size: 27 646 515 843 411 5446 Fragments arranged by size: 5446 843 646 515 411 27 PshAI GACnn'nnGTC Cuts at: 1613 3283 1613 Size: 1670 6218 PsiI TTA'TAA Cuts at: 5458 5458 Size: 7888 PspOMI G'GGCC_C Cuts at: 3348 3348 Size: 7888 PspXI vC'TCGA_Gb Cuts at: 2063 2063 Size: 7888 PstI C_TGCA'G Cuts at: 830 2762 3304 3825 3977 4297 4410 4574 4916 Size: 1932 542 521 152 320 113 164 342 Cuts at: 4916 5240 830 Size: 324 3478 Fragments arranged by size: 3478 1932 542 521 342 324 320 164 152 113 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7888 PvuII CAG'CTG Cuts at: 2179 2814 3256 3849 4435 2179 Size: 635 442 593 586 5632 Fragments arranged by size: 5632 635 593 586 442 SacI G_AGCT'C Cuts at: 2850 2901 2850 Size: 51 7837 SalI G'TCGA_C Cuts at: 1936 5230 1936 Size: 3294 4594 SanDI GG'GwC_CC Cuts at: 2927 2927 Size: 7888 SapI GCTCTTCn'nnn_ Cuts at: 2726 2726 Size: 7888 SbfI CC_TGCA'GG Cuts at: 5240 5240 Size: 7888 ScaI AGT'ACT Cuts at: 6136 7848 6136 Size: 1712 6176 SexAI A'CCwGG_T Cuts at: 2283 2302 2283 Size: 19 7869 SfcI C'TryA_G Cuts at: 826 1045 2389 2758 3300 3532 3821 3973 4012 Size: 219 1344 369 542 232 289 152 39 Cuts at: 4012 4105 4293 4406 4570 4912 5236 6371 7052 Size: 93 188 113 164 342 324 1135 681 Cuts at: 7052 7243 826 Size: 191 1471 Fragments arranged by size: 1471 1344 1135 681 542 369 342 324 289 232 219 191 188 164 152 113 93 39 SfoI GGC'GCC Cuts at: 4006 4006 Size: 7888 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7888 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7888 SmaI CCC'GGG Cuts at: 1872 5215 1872 Size: 3343 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2063 2900 3547 4846 5992 6893 Size: 197 926 120 837 647 1299 1146 901 Cuts at: 6893 7155 820 Size: 262 1553 Fragments arranged by size: 1553 1299 1146 926 901 837 647 262 197 120 SnaBI TAC'GTA Cuts at: 493 5679 493 Size: 5186 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7888 SphI G_CATG'C Cuts at: 1132 2797 5246 1132 Size: 1665 2449 3774 Fragments arranged by size: 3774 2449 1665 SspI AAT'ATT Cuts at: 5 52 5812 5 Size: 47 5760 2081 Fragments arranged by size: 5760 2081 47 StuI AGG'CCT Cuts at: 1517 2210 1517 Size: 693 7195 StyI C'CwwG_G Cuts at: 513 1065 2443 2725 3393 3834 4956 5032 5327 Size: 552 1378 282 668 441 1122 76 295 Cuts at: 5327 513 Size: 3074 Fragments arranged by size: 3074 1378 1122 668 552 441 295 282 76 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2204 3217 3385 3656 4578 5021 5915 Size: 1255 624 1013 168 271 922 443 894 Cuts at: 5915 5932 6085 6270 6715 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1013 922 894 624 445 443 271 185 168 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3306 3576 6134 Size: 274 80 33 51 1443 1329 270 2558 Cuts at: 6134 7846 96 Size: 1712 138 Fragments arranged by size: 2558 1712 1443 1329 274 270 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3935 3967 7848 7880 3935 Size: 32 3881 32 3943 Fragments arranged by size: 3943 3881 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2399 1316 Size: 1083 6805 XbaI T'CTAG_A Cuts at: 5224 5224 Size: 7888 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2208 3724 4486 2208 Size: 1516 762 5610 Fragments arranged by size: 5610 1516 762 XhoI C'TCGA_G Cuts at: 1943 2063 1943 Size: 120 7768 XmaI C'CCGG_G Cuts at: 1870 5213 1870 Size: 3343 4545 XmnI GAAnn'nnTTC Cuts at: 2084 2720 3500 6017 2084 Size: 636 780 2517 3955 Fragments arranged by size: 3955 2517 780 636 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3285 3475 5696 276 Size: 53 83 186 936 1751 190 2221 2468 Fragments arranged by size: 2468 2221 1751 936 190 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BfrBI BsiWI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PsrI RsrII SacII SfiI SrfI SwaI