################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI Alw44I AlwI AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsgI BsiWI BsmI BspEI BspHI BssHII BssSI Bst1107I BstBI BstXI BstYI BstZ17I Bsu36I CelII CpoI DraI DraIII EaeI EagI EclXI Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FseI HpaI KasI KpnI KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SbfI ScaI SexAI SfiI SfoI SfuI SnaBI SphI Sse8387I SwaI TfiI Tsp509I Tth111I XbaI XcmI XhoII XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccI 1413 GTCGAC GT^[AC][GT]AC AcyI 175 GGCGTC G[GA]^CG[CT]C AflIII 671 ACATGT A^C[GA][CT]GT AhdI 1405 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 742 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 335 AGCGCT AGC^GCT ApaI 857 GGGCCC GGGCC^C AspEI 1405 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1194 TGCGCA TGC^GCA BanI 133 GGCACC G^G[CT][GA]CC BsaHI 175 GGCGTC G[GA]^CG[CT]C BsaI 418 GAGACC GGTCTC(1/5) BsiEI 1415 CGACCG CG[GA][CT]^CG BsmBI 1301 CGTCTC CGTCTC(1/5) Bsp1407I 668 TGTACA T^GTACA BspDI 901 ATCGAT AT^CGAT BspLU11I 671 ACATGT A^CATGT BspMI 746 ACCTGC ACCTGC(4/8) BsrBI 3 GAGCGG CCGCTC(-3/-3) BsrDI 377 GCAATG GCAATG(2/0) BsrFI 136 ACCGGC [GA]^CCGG[CT] BsrGI 668 TGTACA T^GTACA BstAPI 483 GCAGATCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 297 GGTGACC G^GT[ACGT]ACC BstPI 297 GGTGACC G^GT[ACGT]ACC Cfr10I 136 ACCGGC [GA]^CCGG[CT] ClaI 901 ATCGAT AT^CGAT DrdI 763 GACACTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 164 CCATGG C^C[GA][CT]GG Eam1105I 1405 GACTGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 335 AGCGCT AGC^GCT EcoNI 16 CCTTCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 297 GGTGACC G^GT[ACGT]ACC FspI 1194 TGCGCA TGC^GCA HaeII 335 AGCGCT [GA]GCGC^[CT] HgaI 176 GCGTC GACGC(5/10) Hin1I 175 GGCGTC G[GA]^CG[CT]C HindIII 1376 AAGCTT A^AGCTT HinfI 1389 GACTC G^A[ACGT]TC NcoI 164 CCATGG C^CATGG NspI 671 ACATGT [GA]CATG^[CT] PaeR7I 186 CTCGAG C^TCGAG PleI 1389 GACTC GAGTC(4/5) PpuMI 1336 AGGTCCC [GA]G^G[AT]CC[CT] PshAI 300 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 481 CTGCAG CTGCA^G SalI 1413 GTCGAC G^TCGAC SapI 681 GCTCTTC GCTCTTC(1/4) SfcI 481 CTGCAG C^T[GA][CT]AG SgrAI 135 CACCGGCG C[GA]^CCGG[CT]G SpeI 429 ACTAGT A^CTAGT SspBI 668 TGTACA T^GTACA SspI 511 AATATT AAT^ATT StuI 1187 AGGCCT AGG^CCT XhoI 186 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BsmFI 965 GGGAC GGGAC(10/14) 1338 GTCCC DpnII 486 GATC ^GATC 993 GATC EarI 682 CTCTTC CTCTTC(1/4) 1264 CTCTTC HincII 639 GTTGAC GT[CT]^[GA]AC 1413 GTCGAC HindII 639 GTTGAC GT[CT]^[GA]AC 1413 GTCGAC Ksp632I 682 CTCTTC CTCTTC(1/4) 1264 CTCTTC MboI 486 GATC ^GATC 993 GATC MseI 1076 TTAA T^TAA 1381 TTAA MslI 363 CATCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 996 CATCAAGGTG NdeII 486 GATC ^GATC 993 GATC PflMI 342 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 981 CCAGCTGGTGG Sau3AI 486 GATC ^GATC 993 GATC SfaNI 1420 GATGC GCATC(5/9) 151 GCATC SmaI 265 CCCGGG CCC^GGG 1019 CCCGGG Tru9I 1076 TTAA T^TAA 1381 TTAA Van91I 342 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 981 CCAGCTGGTGG XmaCI 265 CCCGGG C^CCGGG 1019 CCCGGG XmaI 265 CCCGGG C^CCGGG 1019 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AccII AciI AfaI AluI AspHI AvaI AvaII BanII BbvI BcnI BfaI BglI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeI MaeIII MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PvuII RsaI SacI Sau96I ScrFI SmlI StyI TaqI TseI Tsp45I TspRI TthHB8I