Restriction map for
pFN21AE3647
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# NO CUT ENZYMES ( 184 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstYI
BstZ17I CelII Cfr10I ClaI CpoI
DpnII DraI DraII DraIII EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO109I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MamI MboI MboII
MfeI MflI MluI MluNI MroI
MscI MslI MspA1I MunI MvnI
NaeI NarI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfcI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tsp509I Tth111I TthHB8I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AciI 125 CCGC CCGC(-3/-1)
AfaI 13 GTAC GT^AC
AlwNI 177 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 265 CCTGAGG CC^T[ACGT]AGG
AspHI 222 GAGCAC G[AT]GC[AT]^C
AvaII 261 GGACC G^G[AT]CC
BmyI 222 GAGCAC G[AGT]GC[ACT]^C
BpmI 219 CTGGAG CTGGAG(16/14)
BsgI 148 GTGCAG GTGCAG(16/14)
BsiHKAI 222 GAGCAC G[AT]GC[AT]^C
BsiYI 176 CCAGGGGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 176 CCAGGGGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 156 GTCCC GGGAC(10/14)
Bsp1286I 222 GAGCAC G[AGT]GC[ACT]^C
BsrI 218 ACTGG ACTGG(1/-1)
BstEII 186 GGTGACC G^GT[ACGT]ACC
BstPI 186 GGTGACC G^GT[ACGT]ACC
Bsu36I 265 CCTGAGG CC^T[ACGT]AGG
CfoI 37 GCGC GCG^C
DrdI 262 GACCCTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 287 CCGTGG C^C[GA][CT]GG
Eco81I 265 CCTGAGG CC^T[ACGT]AGG
EcoO65I 186 GGTGACC G^GT[ACGT]ACC
EcoT14I 128 CCAAGG C^C[AT][AT]GG
HaeII 36 GGCGCT [GA]GCGC^[CT]
HapII 9 CCGG C^CGG
HhaI 37 GCGC GCG^C
HinP1I 37 GCGC G^CGC
HinfI 154 GAGTC G^A[ACGT]TC
HpaII 9 CCGG C^CGG
HphI 186 GGTGA GGTGA(8/7)
MseI 230 TTAA T^TAA
MspI 9 CCGG C^CGG
NlaIV 261 GGACCC GG[ACGT]^[ACGT]CC
PflMI 176 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 154 GAGTC GAGTC(4/5)
RsaI 13 GTAC GT^AC
SfiI 165 GGCCCAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 8 CCCGGG CCC^GGG
StyI 128 CCAAGG C^C[AT][AT]GG
Tru9I 230 TTAA T^TAA
Tsp45I 187 GTGAC ^GT[GC]AC
TspRI 137 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 176 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 8 CCCGGG C^CCGGG
XmaI 8 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 13 enzymes) #
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AluI 57 AGCT AG^CT
88 AGCT
AvaI 8 CCCGGG C^[CT]CG[GA]G
53 CCCGAG
BcnI 8 CCCGG CC^[GC]GG
9 CCGGG
BglI 28 GCCCTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
166 GCCCAGCAGGC
BsoBI 8 CCCGGG C^[CT]CG[GA]G
53 CCCGAG
BstF5I 72 GGATG GGATG(2/0)
132 GGATG
Cfr13I 165 GGCCC G^G[ACGT]CC
261 GGACC
FokI 72 GGATG GGATG(9/13)
132 GGATG
MaeIII 101 GTTAC ^GT[ACGT]AC
187 GTGAC
NciI 8 CCCGG CC^[GC]GG
9 CCGGG
NlaIII 93 CATG CATG^
105 CATG
Sau96I 165 GGCCC G^G[ACGT]CC
261 GGACC
SfaNI 73 GATGC GCATC(5/9)
133 GATGC
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
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BbvI BsaJI BssKI BstNI Cac8I
DdeI EcoRII Fnu4HI HaeIII ItaI
MnlI MvaI MwoI ScrFI TseI