################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BglI BglII BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI DsaI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MaeIII MamI MfeI MluI MroI MseI MslI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tsp45I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AfaI 433 GTAC GT^AC AluI 189 AGCT AG^CT Aor51HI 69 AGCGCT AGC^GCT AspHI 301 GAGCAC G[AT]GC[AT]^C AvaI 50 CCCGAG C^[CT]CG[GA]G AvaII 47 GGACC G^G[AT]CC AvrII 43 CCTAGG C^CTAGG BalI 440 TGGCCA TGG^CCA BamHI 228 GGATCC G^GATCC BclI 446 TGATCA T^GATCA BcnI 12 CCGGG CC^[GC]GG BfaI 44 CTAG C^TAG BlnI 43 CCTAGG C^CTAGG BlpI 124 GCTCAGC GC^T[ACGT]AGC BmyI 301 GAGCAC G[AGT]GC[ACT]^C BpmI 298 CTGGAG CTGGAG(16/14) Bpu1102I 124 GCTCAGC GC^T[ACGT]AGC BsgI 400 GTGCAG GTGCAG(16/14) BsiEI 61 CGGCCG CG[GA][CT]^CG BsiHKAI 301 GAGCAC G[AT]GC[AT]^C BsmBI 37 CGTCTC CGTCTC(1/5) BsmFI 166 GGGAC GGGAC(10/14) BsoBI 50 CCCGAG C^[CT]CG[GA]G Bsp1286I 301 GAGCAC G[AGT]GC[ACT]^C BsrI 254 ACTGG ACTGG(1/-1) BssHII 21 GCGCGC G^CGCGC BssSI 112 CACGAG CACGAG(-5/-1) BstXI 335 CCAATCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 124 GCTCAGC GC^T[ACGT]AGC DdeI 125 CTCAG C^T[ACGT]AG EagI 61 CGGCCG C^GGCCG EarI 131 CTCTTC CTCTTC(1/4) EclXI 61 CGGCCG C^GGCCG Eco47III 69 AGCGCT AGC^GCT Eco52I 61 CGGCCG C^GGCCG FauI 33 CCCGC CCCGC(4/6) FbaI 446 TGATCA T^GATCA HgaI 36 GCGTC GACGC(5/10) Ksp632I 131 CTCTTC CTCTTC(1/4) MaeI 44 CTAG C^TAG MluNI 440 TGGCCA TGG^CCA MscI 440 TGGCCA TGG^CCA MspA1I 188 CAGCTG C[AC]G^C[GT]G NciI 12 CCGGG CC^[GC]GG PpuMI 46 AGGACCC [GA]G^G[AT]CC[CT] PvuII 188 CAGCTG CAG^CTG RsaI 433 GTAC GT^AC SgrAI 24 CGCCGGCG C[GA]^CCGG[CT]G StuI 271 AGGCCT AGG^CCT TaqI 236 TCGA T^CGA Tsp509I 197 AATT ^AATT TspRI 510 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 236 TCGA T^CGA XcmI 386 CCAACTGCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccII 22 CGCG CG^CG 35 CGCG BbvI 67 GCAGC GCAGC(8/12) 402 GCAGC BsrFI 25 GCCGGC [GA]^CCGG[CT] 63 GCCGGC BstF5I 468 CATCC GGATG(2/0) 429 GGATG BstUI 22 CGCG CG^CG 35 CGCG BstYI 228 GGATCC [GA]^GATC[CT] 374 AGATCC Cfr10I 25 GCCGGC [GA]^CCGG[CT] 63 GCCGGC DraII 46 AGGACCC [GA]G^G[ACGT]CC[CT] 116 AGGCCCT EaeI 61 CGGCCG [CT]^GGCC[GA] 440 TGGCCA EcoO109I 46 AGGACCC [GA]G^G[ACGT]CC[CT] 116 AGGCCCT EcoT14I 43 CCTAGG C^C[AT][AT]GG 341 CCTTGG FokI 468 CATCC GGATG(9/13) 429 GGATG HaeII 69 AGCGCT [GA]GCGC^[CT] 94 GGCGCT HphI 171 GGTGA GGTGA(8/7) 369 TCACC MboII 174 GAAGA GAAGA(8/7) 132 TCTTC MflI 228 GGATCC [GA]^GATC[CT] 374 AGATCC MvnI 22 CGCG CG^CG 35 CGCG MwoI 23 GCGCCGGCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 63 GCCGGCAGCGC NaeI 25 GCCGGC GCC^GGC 63 GCCGGC NgoMIV 25 GCCGGC G^CCGGC 63 GCCGGC NlaIII 261 CATG CATG^ 444 CATG NlaIV 47 GGACCC GG[ACGT]^[ACGT]CC 228 GGATCC PleI 366 GACTC GAGTC(4/5) 53 GAGTC PshAI 48 GACCCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 186 GACAGCTGTC StyI 43 CCTAGG C^C[AT][AT]GG 341 CCTTGG TfiI 105 GAATC G^A[AT]TC 207 GAATC TseI 67 GCAGC G^C[AT]GC 402 GCAGC XhoII 228 GGATCC [GA]^GATC[CT] 374 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AciI AlwI BsaJI BseRI BsiYI BslI BsmAI BssKI BstNI Cac8I CfoI Cfr13I DpnII EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboI MnlI MspI MvaI NdeII Sau3AI Sau96I ScrFI