################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MseI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccI 682 GTCTAC GT^[AC][GT]AC AgeI 586 ACCGGT A^CCGGT AlwNI 115 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 456 GAGCAC G[AT]GC[AT]^C BamHI 567 GGATCC G^GATCC BbsI 576 GAAGAC GAAGAC(2/6) BcnI 605 CCGGG CC^[GC]GG BglI 375 GCCTCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 576 GAAGAC GAAGAC(2/6) BsaI 693 GGTCTC GGTCTC(1/5) BsaWI 586 ACCGGT [AT]^CCGG[AT] BsiHKAI 456 GAGCAC G[AT]GC[AT]^C BsmBI 520 CGTCTC CGTCTC(1/5) BsrBI 247 GAGCGG CCGCTC(-3/-3) BsrFI 586 ACCGGT [GA]^CCGG[CT] BsrI 510 CCAGT ACTGG(1/-1) BssSI 616 CTCGTG CACGAG(-5/-1) BstYI 567 GGATCC [GA]^GATC[CT] Cac8I 433 GCTGGC GC[ACGT]^[ACGT]GC CfoI 732 GCGC GCG^C Cfr10I 586 ACCGGT [GA]^CCGG[CT] DsaI 528 CCATGG C^C[GA][CT]GG FauI 92 CCCGC CCCGC(4/6) HaeII 731 AGCGCC [GA]GCGC^[CT] HhaI 732 GCGC GCG^C HinP1I 732 GCGC G^CGC MaeIII 214 GTCAC ^GT[ACGT]AC MflI 567 GGATCC [GA]^GATC[CT] NciI 605 CCGGG CC^[GC]GG NcoI 528 CCATGG C^CATGG PinAI 586 ACCGGT A^CCGGT SfaNI 152 GCATC GCATC(5/9) Tsp45I 214 GTCAC ^GT[GC]AC Tsp509I 535 AATT ^AATT XhoII 567 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwI 568 GATCC GGATC(4/5) 567 GGATC ApaI 224 GGGCCC GGGCC^C 610 GGGCCC AvaII 251 GGACC G^G[AT]CC 359 GGACC BanII 224 GGGCCC G[GA]GC[CT]^C 610 GGGCCC BfaI 157 CTAG C^TAG 389 CTAG BpmI 674 CTCCAG CTGGAG(16/14) 340 CTGGAG BseRI 13 CTCCTC GAGGAG(10/8) 55 GAGGAG BsgI 423 GTGCAG GTGCAG(16/14) 496 GTGCAG DpnII 60 GATC ^GATC 568 GATC HapII 587 CCGG C^CGG 605 CCGG HpaII 587 CCGG C^CGG 605 CCGG HphI 405 GGTGA GGTGA(8/7) 215 TCACC MaeI 157 CTAG C^TAG 389 CTAG MaeII 519 ACGT A^CGT 629 ACGT MboI 60 GATC ^GATC 568 GATC MslI 153 CATCCTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 363 CACAGCTGTG MspI 587 CCGG C^CGG 605 CCGG NdeII 60 GATC ^GATC 568 GATC PstI 334 CTGCAG CTGCA^G 475 CTGCAG Sau3AI 60 GATC ^GATC 568 GATC SfcI 334 CTGCAG C^T[GA][CT]AG 475 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AciI AluI BbvI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI Cfr13I DdeI DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MboII MnlI MspA1I MvaI MwoI NlaIII NlaIV PleI PvuII Sau96I ScrFI StyI TseI TspRI