################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## Acc65I AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BfrI BglII BlnI BsaBI BsaWI BseAI BsiEI BsiWI BsmI BspDI BspEI BspHI BspLU11I BssHII BssSI BstBI BstEII BstPI BstUI Bsu36I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XhoI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AcsI 1083 AAATTT [GA]^AATT[CT] Aor51HI 117 AGCGCT AGC^GCT ApoI 1083 AAATTT [GA]^AATT[CT] AvaI 1694 CCCGGG C^[CT]CG[GA]G BbsI 456 GAAGAC GAAGAC(2/6) BbvCI 1805 CCTCAGC CCTCAGC(-5/-2) BglI 465 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 456 GAAGAC GAAGAC(2/6) BsaAI 730 TACGTA [CT]AC^GT[GA] BsgI 784 GTGCAG GTGCAG(16/14) BsmBI 366 CGTCTC CGTCTC(1/5) BsoBI 1694 CCCGGG C^[CT]CG[GA]G Bsp1407I 176 TGTACA T^GTACA BsrBI 1737 CCGCTC CCGCTC(-3/-3) BsrDI 446 GCAATG GCAATG(2/0) BsrFI 377 ACCGGC [GA]^CCGG[CT] BsrGI 176 TGTACA T^GTACA Bst1107I 600 GTATAC GTA^TAC BstZ17I 600 GTATAC GTA^TAC CfoI 118 GCGC GCG^C Cfr10I 377 ACCGGC [GA]^CCGG[CT] Eco47III 117 AGCGCT AGC^GCT HaeII 117 AGCGCT [GA]GCGC^[CT] HhaI 118 GCGC GCG^C HinP1I 118 GCGC G^CGC MseI 1357 TTAA T^TAA NspI 1590 GCATGT [GA]CATG^[CT] PshAI 927 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 1707 GCTCTTC GCTCTTC(1/4) SmaI 1694 CCCGGG CCC^GGG SnaBI 730 TACGTA TAC^GTA SspBI 176 TGTACA T^GTACA Tru9I 1357 TTAA T^TAA XbaI 999 TCTAGA T^CTAGA XmaCI 1694 CCCGGG C^CCGGG XmaI 1694 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AatII 364 GACGTC GACGT^C 751 GACGTC AlwI 684 GATCC GGATC(4/5) 294 GGATC BanI 400 GGTGCC G^G[CT][GA]CC 836 GGTGCC BclI 1334 TGATCA T^GATCA 1416 TGATCA BlpI 1575 GCTGAGC GC^T[ACGT]AGC 1839 GCTGAGC Bpu1102I 1575 GCTGAGC GC^T[ACGT]AGC 1839 GCTGAGC BsaI 63 GGTCTC GGTCTC(1/5) 1531 GGTCTC BspMI 131 ACCTGC ACCTGC(4/8) 1835 ACCTGC BsrI 277 CCAGT ACTGG(1/-1) 779 CCAGT BstAPI 315 GCAAAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 336 GCAGACACTGC BstYI 294 GGATCT [GA]^GATC[CT] 683 AGATCC CelII 1575 GCTGAGC GC^T[ACGT]AGC 1839 GCTGAGC DsaI 654 CCACGG C^C[GA][CT]GG 1434 CCGTGG FbaI 1334 TGATCA T^GATCA 1416 TGATCA HgaI 270 GACGC GACGC(5/10) 480 GACGC MflI 294 GGATCT [GA]^GATC[CT] 683 AGATCC PleI 1612 GACTC GAGTC(4/5) 1815 GAGTC PstI 706 CTGCAG CTGCA^G 1666 CTGCAG PvuII 223 CAGCTG CAG^CTG 310 CAGCTG SfaNI 201 GATGC GCATC(5/9) 1026 GCATC SfcI 706 CTGCAG C^T[GA][CT]AG 1666 CTGCAG Tsp509I 796 AATT ^AATT 1084 AATT XcmI 485 CCACACCCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 920 CCACAAGGACTGTGG XhoII 294 GGATCT [GA]^GATC[CT] 683 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AccI AciI AcyI AfaI AluI AlwNI AspHI AvaII BalI BanII BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII Hin1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PpuMI RsaI SacI Sau3AI Sau96I ScrFI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I