(Circular) (Six-base) MAPSORT of: pFN21AE4542.seq Check: 5000 from: 1 to: 6583 pFN21AE4542 6583 bp With 182 enzymes: SgfI * March 28, 2012 14:11 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2373 2760 4393 278 Size: 53 83 186 936 837 387 1633 2468 Fragments arranged by size: 2468 1633 936 837 387 186 83 53 AccI GT'mk_AC Cuts at: 1937 2606 2733 2820 3926 1937 Size: 669 127 87 1106 4594 Fragments arranged by size: 4594 1106 669 127 87 Acc65I G'GTAC_C Cuts at: 3904 3904 Size: 6583 AclI AA'CG_TT Cuts at: 1563 4710 5083 1563 Size: 3147 373 3063 Fragments arranged by size: 3147 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2480 4646 6030 6298 1632 Size: 848 2166 1384 268 1917 Fragments arranged by size: 2166 1917 1384 848 268 AfeI AGC'GCT Cuts at: 2124 2124 Size: 6583 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6386 AflIII A'CryG_T Cuts at: 1203 5482 6459 1203 Size: 4279 977 1327 Fragments arranged by size: 4279 1327 977 AgeI A'CCGG_T Cuts at: 5414 5414 Size: 6583 AhdI GACnn_n'nnGTC Cuts at: 5312 5312 Size: 6583 AleI CACnn'nnGTG Cuts at: 1166 2166 1166 Size: 1000 5583 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2736 1184 Size: 1552 5031 AlwNI CAG_nnn'CTG Cuts at: 2347 3613 3841 4326 5898 2347 Size: 1266 228 485 1572 3032 Fragments arranged by size: 3032 1572 1266 485 228 ApaLI G'TGCA_C Cuts at: 4640 5796 4640 Size: 1156 5427 ApoI r'AATT_y Cuts at: 1493 3088 3892 4118 4270 6379 1493 Size: 1595 804 226 152 2109 1697 Fragments arranged by size: 2109 1697 1595 804 226 152 AseI AT'TA_AT Cuts at: 160 1032 5137 160 Size: 872 4105 1606 Fragments arranged by size: 4105 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6583 AvaI C'yCGr_G Cuts at: 1870 1943 3699 3908 1870 Size: 73 1756 209 4545 Fragments arranged by size: 4545 1756 209 73 BamHI G'GATC_C Cuts at: 3913 4280 3913 Size: 367 6216 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2405 2841 3904 5359 618 Size: 325 217 627 618 436 1063 1455 1842 Fragments arranged by size: 1842 1455 1063 627 618 436 325 217 BanII G_rGCy'C Cuts at: 1391 2253 2439 2481 2541 2553 3177 3189 3216 Size: 862 186 42 60 12 624 12 27 Cuts at: 3216 3243 3255 3282 3470 3902 1391 Size: 27 12 27 188 432 4072 Fragments arranged by size: 4072 862 624 432 188 186 60 42 27 27 27 12 12 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2468 928 Size: 971 569 5043 Fragments arranged by size: 5043 971 569 BbvCI CC'TCA_GC Cuts at: 3811 3811 Size: 6583 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4774 4808 1903 Size: 34 50 34 2753 34 3678 Fragments arranged by size: 3678 2753 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4475 5685 4475 Size: 1210 5373 BclI T'GATC_A Cuts at: 1548 3339 3421 1548 Size: 1791 82 4710 Fragments arranged by size: 4710 1791 82 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2476 5194 136 Size: 107 122 71 631 605 804 2718 1525 Fragments arranged by size: 2718 1525 804 631 605 122 107 71 BglII A'GATC_T Cuts at: 6578 6578 Size: 6583 BlpI GC'TnA_GC Cuts at: 3581 3845 4000 3581 Size: 264 155 6164 Fragments arranged by size: 6164 264 155 Bme1580I G_kGCm'C Cuts at: 1790 2410 2846 3699 4644 5800 1790 Size: 620 436 853 945 1156 2573 Fragments arranged by size: 2573 1156 945 853 620 436 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6583 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4314 5272 442 Size: 416 53 83 399 24 2897 958 1753 Fragments arranged by size: 2897 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6583 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3262 3262 Size: 6583 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2115 2246 2522 2585 3230 3454 3686 3730 Size: 876 131 276 63 645 224 232 44 Cuts at: 3730 5243 1239 Size: 1513 2579 Fragments arranged by size: 2579 1513 876 645 276 232 224 131 63 44 Bpu10I CC'TnA_GC Cuts at: 3811 3811 Size: 6583 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4708 5573 5871 4708 Size: 865 298 5420 Fragments arranged by size: 5420 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2074 3542 5246 882 Size: 617 575 1468 1704 2219 Fragments arranged by size: 2219 1704 1468 617 575 BsaAI yAC'GTr Cuts at: 493 1204 2737 4374 493 Size: 711 1533 1637 2702 Fragments arranged by size: 2702 1637 1533 711 BsaBI GATnn'nnATC Cuts at: 6577 6577 Size: 6583 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2370 2486 2757 4390 Size: 53 83 186 936 837 116 271 1633 Cuts at: 4390 4772 5407 275 Size: 382 635 1451 Fragments arranged by size: 1633 1451 936 837 635 382 271 186 116 83 53 BsaWI w'CCGG_w Cuts at: 5016 5414 5688 5835 6246 5016 Size: 398 274 147 411 5353 Fragments arranged by size: 5353 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2174 2204 3420 3450 1178 Size: 30 165 30 771 30 1216 30 4311 Fragments arranged by size: 4311 1216 771 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2003 2006 2576 3013 3500 1186 Size: 817 3 570 437 487 4269 Fragments arranged by size: 4269 817 570 487 437 3 BseYI C'CCAG_C Cuts at: 2287 2546 3260 3585 5786 6282 2287 Size: 259 714 325 2201 496 2588 Fragments arranged by size: 2588 2201 714 496 325 259 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2810 1163 Size: 573 1074 4936 Fragments arranged by size: 4936 1074 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4033 4794 5822 1540 Size: 275 186 2032 761 1028 2301 Fragments arranged by size: 2301 2032 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2253 2439 2541 3450 3470 3851 4644 4729 5800 Size: 186 102 909 20 381 793 85 1071 Cuts at: 5800 2253 Size: 3036 Fragments arranged by size: 3036 1071 909 793 381 186 102 85 20 BsmI GAATG_Cn' Cuts at: 4094 4187 4094 Size: 93 6490 BsmBI CGTCTCn'nnnn_ Cuts at: 2377 2377 Size: 6583 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2253 2410 2439 2481 2541 2553 2846 Size: 399 463 157 29 42 60 12 293 Cuts at: 2846 3177 3189 3216 3243 3255 3282 3450 3470 Size: 331 12 27 27 12 27 168 20 Cuts at: 3470 3699 3851 3902 4644 4729 5800 1391 Size: 229 152 51 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 463 399 331 293 229 168 157 152 85 60 51 42 29 27 27 27 20 12 12 12 BspEI T'CCGG_A Cuts at: 6246 6246 Size: 6583 BspHI T'CATG_A Cuts at: 4472 6202 4472 Size: 1730 4853 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2145 3849 3938 844 Size: 1301 1704 89 3489 Fragments arranged by size: 3489 1704 1301 89 BsrBI CCG'CTC Cuts at: 1394 1823 3744 4470 1394 Size: 429 1921 726 3507 Fragments arranged by size: 3507 1921 726 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2458 5078 5252 65 Size: 1183 1210 2620 174 1396 Fragments arranged by size: 2620 1396 1210 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2382 5227 5414 1700 Size: 110 572 2845 187 2869 Fragments arranged by size: 2869 2845 572 187 110 BsrGI T'GTAC_A Cuts at: 96 2181 96 Size: 2085 4498 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6583 BssSI C'ACGA_G Cuts at: 4643 5655 4643 Size: 1012 5571 BstAPI GCAn_nnn'nTGC Cuts at: 794 2326 2347 794 Size: 1532 21 5030 Fragments arranged by size: 5030 1532 21 BstBI TT'CG_AA Cuts at: 5387 5387 Size: 6583 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6583 BstXI CCAn_nnnn'nTGG Cuts at: 2270 3078 3664 2270 Size: 808 586 5189 Fragments arranged by size: 5189 808 586 BstYI r'GATC_y Cuts at: 1972 2299 2688 3913 4280 4665 4682 6134 6220 Size: 327 389 1225 367 385 17 1452 86 Cuts at: 6220 6232 6578 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1225 389 385 367 346 327 86 17 12 BstZ17I GTA'TAC Cuts at: 2607 2607 Size: 6583 BtgI C'CryG_G Cuts at: 513 1065 2659 3439 513 Size: 552 1594 780 3657 Fragments arranged by size: 3657 1594 780 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3283 487 Size: 683 56 2057 3787 Fragments arranged by size: 3787 2057 683 56 BtsI GCAGTG_nn' Cuts at: 2345 2798 2994 4099 4893 4913 2345 Size: 453 196 1105 794 20 4015 Fragments arranged by size: 4015 1105 794 453 196 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6583 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2916 2951 529 Size: 35 2352 35 4161 Fragments arranged by size: 4161 2352 35 35 DraI TTT'AAA Cuts at: 3887 3887 Size: 6583 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5590 809 Size: 507 4274 1802 Fragments arranged by size: 4274 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2079 2781 3622 Size: 54 1425 50 275 18 249 702 841 Cuts at: 3622 3775 3871 4030 4919 6548 8 Size: 153 96 159 889 1629 43 Fragments arranged by size: 1629 1425 889 841 702 275 249 159 153 96 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4030 1537 Size: 275 2218 4090 Fragments arranged by size: 4090 2218 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2889 3545 3719 3758 4513 1353 Size: 1536 656 174 39 755 3423 Fragments arranged by size: 3423 1536 755 656 174 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5170 5544 5690 387 Size: 1064 138 3581 374 146 1280 Fragments arranged by size: 3581 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2251 2437 2539 3468 2251 Size: 186 102 929 5366 Fragments arranged by size: 5366 929 186 102 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2115 2246 2480 2522 2585 3230 3454 Size: 393 483 131 234 42 63 645 224 Cuts at: 3454 3686 3730 4646 5243 6030 6298 1239 Size: 232 44 916 597 787 268 1524 Fragments arranged by size: 1524 916 787 645 597 483 393 268 234 232 224 131 63 44 42 EcoO109I rG'GnC_Cy Cuts at: 2292 2631 3610 3785 3807 3838 2292 Size: 339 979 175 22 31 5037 Fragments arranged by size: 5037 979 339 175 31 22 EcoRI G'AATT_C Cuts at: 3892 4270 3892 Size: 378 6205 FspI TGC'GCA Cuts at: 5089 5089 Size: 6583 HaeII r_GCGC'y Cuts at: 1678 2126 5730 1678 Size: 448 3604 2531 Fragments arranged by size: 3604 2531 448 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2284 2316 3811 3843 5224 Size: 32 135 32 712 32 1495 32 1381 Cuts at: 5224 5256 5298 5330 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1495 1381 712 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3927 4173 669 Size: 988 281 1989 246 3079 Fragments arranged by size: 3079 1989 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6583 HpaI GTT'AAC Cuts at: 4173 4173 Size: 6583 KpnI G_GTAC'C Cuts at: 3908 3908 Size: 6583 MfeI C'AATT_G Cuts at: 4182 4182 Size: 6583 MluI A'CGCG_T Cuts at: 6459 6459 Size: 6583 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3059 5697 5881 1305 Size: 1754 2638 184 2007 Fragments arranged by size: 2638 2007 1754 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2081 2783 3624 3873 10 Size: 54 1425 343 249 702 841 249 2720 Fragments arranged by size: 2720 1425 841 702 343 249 249 54 MslI CAynn'nnrTG Cuts at: 518 1166 2166 3076 3320 4541 4900 5059 518 Size: 648 1000 910 244 1221 359 159 2042 Fragments arranged by size: 2042 1221 1000 910 648 359 244 159 MspA1I CmG'CkG Cuts at: 1606 2230 2317 2546 3999 4676 5824 6069 1606 Size: 624 87 229 1453 677 1148 245 2120 Fragments arranged by size: 2120 1453 1148 677 624 245 229 87 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6031 NdeI CA'TA_TG Cuts at: 387 387 Size: 6583 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6583 NotI GC'GGCC_GC Cuts at: 4030 4030 Size: 6583 NspI r_CATG'y Cuts at: 1132 1229 3599 3941 5486 1132 Size: 97 2370 342 1545 2229 Fragments arranged by size: 2370 2229 1545 342 97 PasI CC'CwG_GG Cuts at: 1348 2474 2970 3517 1348 Size: 1126 496 547 4414 Fragments arranged by size: 4414 1126 547 496 PciI A'CATG_T Cuts at: 5482 5482 Size: 6583 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6583 PfoI T'CCnGG_A Cuts at: 2364 3180 2364 Size: 816 5767 PmeI GTTT'AAAC Cuts at: 3887 3887 Size: 6583 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4652 4684 6165 6197 4652 Size: 32 1481 32 5038 Fragments arranged by size: 5038 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2292 2631 3838 2292 Size: 339 1207 5037 Fragments arranged by size: 5037 1207 339 PshAI GACnn'nnGTC Cuts at: 1613 2936 1613 Size: 1323 5260 PsiI TTA'TAA Cuts at: 4153 4153 Size: 6583 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2165 2197 2165 Size: 32 6551 PstI C_TGCA'G Cuts at: 830 2715 3675 3935 830 Size: 1885 960 260 3478 Fragments arranged by size: 3478 1885 960 260 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6583 PvuII CAG'CTG Cuts at: 2230 2317 2230 Size: 87 6496 SacI G_AGCT'C Cuts at: 2253 2439 2541 3470 2253 Size: 186 102 929 5366 Fragments arranged by size: 5366 929 186 102 SalI G'TCGA_C Cuts at: 1936 3925 1936 Size: 1989 4594 SanDI GG'GwC_CC Cuts at: 2292 2631 2292 Size: 339 6244 SapI GCTCTTCn'nnn_ Cuts at: 3719 3719 Size: 6583 SbfI CC_TGCA'GG Cuts at: 3935 3935 Size: 6583 ScaI AGT'ACT Cuts at: 4831 6543 4831 Size: 1712 4871 SfcI C'TryA_G Cuts at: 826 1045 2711 3671 3931 5066 5747 5938 826 Size: 219 1666 960 260 1135 681 191 1471 Fragments arranged by size: 1666 1471 1135 960 681 260 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6583 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6583 SmaI CCC'GGG Cuts at: 1872 3701 3910 1872 Size: 1829 209 4545 Fragments arranged by size: 4545 1829 209 SmlI C'TyrA_G Cuts at: 820 1017 1943 4687 5588 5850 820 Size: 197 926 2744 901 262 1553 Fragments arranged by size: 2744 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2737 4374 493 Size: 2244 1637 2702 Fragments arranged by size: 2702 2244 1637 SpeI A'CTAG_T Cuts at: 152 152 Size: 6583 SphI G_CATG'C Cuts at: 1132 3941 1132 Size: 2809 3774 SspI AAT'ATT Cuts at: 5 52 4507 5 Size: 47 4455 2081 Fragments arranged by size: 4455 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6583 StyI C'CwwG_G Cuts at: 513 1065 2851 3451 3625 4022 513 Size: 552 1786 600 174 397 3074 Fragments arranged by size: 3074 1786 600 552 397 174 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2509 4610 4627 4780 4965 5410 325 Size: 1255 929 2101 17 153 185 445 1498 Fragments arranged by size: 2101 1498 1255 929 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 4829 6541 Size: 274 80 33 51 1443 204 2648 1712 Cuts at: 6541 96 Size: 138 Fragments arranged by size: 2648 1712 1443 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2734 2766 3092 3124 3420 3452 3672 3704 6543 Size: 32 326 32 296 32 220 32 2839 Cuts at: 6543 6575 2734 Size: 32 2742 Fragments arranged by size: 2839 2742 326 296 220 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6583 XbaI T'CTAG_A Cuts at: 3004 3919 3004 Size: 915 5668 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2497 2932 2497 Size: 435 6148 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6583 XmaI C'CCGG_G Cuts at: 1870 3699 3908 1870 Size: 1829 209 4545 Fragments arranged by size: 4545 1829 209 XmnI GAAnn'nnTTC Cuts at: 4712 4712 Size: 6583 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2371 2758 4391 276 Size: 53 83 186 936 837 387 1633 2468 Fragments arranged by size: 2468 1633 936 837 387 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII SexAI SfiI SfoI SrfI SwaI