(Linear) (Six-base) MAPSORT of: KIEE4542.seq Check: 7298 from: 1 to: 1878 KIEE4542.orf With 182 enzymes: SgfI * March 22, 2012 18:19 .. AatII G_ACGT'C Cuts at: 0 368 755 1878 Size: 368 387 1123 Fragments arranged by size: 1123 387 368 AccI GT'mk_AC Cuts at: 0 601 728 815 1878 Size: 601 127 87 1063 Fragments arranged by size: 1063 601 127 87 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 475 1878 Size: 475 1403 AfeI AGC'GCT Cuts at: 0 119 1878 Size: 119 1759 AleI CACnn'nnGTG Cuts at: 0 161 1878 Size: 161 1717 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 731 1878 Size: 731 1147 AlwNI CAG_nnn'CTG Cuts at: 0 342 1608 1836 1878 Size: 342 1266 228 42 Fragments arranged by size: 1266 342 228 42 ApoI r'AATT_y Cuts at: 0 1083 1878 Size: 1083 795 AvaI C'yCGr_G Cuts at: 0 1694 1878 Size: 1694 184 BanI G'GyrC_C Cuts at: 0 400 836 1878 Size: 400 436 1042 Fragments arranged by size: 1042 436 400 BanII G_rGCy'C Cuts at: 0 248 434 476 536 548 1172 1184 1211 Size: 248 186 42 60 12 624 12 27 Cuts at: 1211 1238 1250 1277 1465 1878 Size: 27 12 27 188 413 Fragments arranged by size: 624 413 248 188 186 60 42 27 27 27 12 12 12 BbsI GAAGACnn'nnnn_ Cuts at: 0 463 1878 Size: 463 1415 BbvCI CC'TCA_GC Cuts at: 0 1806 1878 Size: 1806 72 BclI T'GATC_A Cuts at: 0 1334 1416 1878 Size: 1334 82 462 Fragments arranged by size: 1334 462 82 BglI GCCn_nnn'nGGC Cuts at: 0 471 1878 Size: 471 1407 BlpI GC'TnA_GC Cuts at: 0 1576 1840 1878 Size: 1576 264 38 Fragments arranged by size: 1576 264 38 Bme1580I G_kGCm'C Cuts at: 0 405 841 1694 1878 Size: 405 436 853 184 Fragments arranged by size: 853 436 405 184 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1257 1878 Size: 1257 621 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 110 241 517 580 1225 1449 1681 1725 Size: 110 131 276 63 645 224 232 44 Cuts at: 1725 1878 Size: 153 Fragments arranged by size: 645 276 232 224 153 131 110 63 44 Bpu10I CC'TnA_GC Cuts at: 0 1806 1878 Size: 1806 72 BsaI GGTCTCn'nnnn_ Cuts at: 0 69 1537 1878 Size: 69 1468 341 Fragments arranged by size: 1468 341 69 BsaAI yAC'GTr Cuts at: 0 732 1878 Size: 732 1146 BsaHI Gr'CG_yC Cuts at: 0 365 481 752 1878 Size: 365 116 271 1126 Fragments arranged by size: 1126 365 271 116 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 169 199 1415 1445 1878 Size: 169 30 1216 30 433 Fragments arranged by size: 1216 433 169 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1 571 1008 1495 1878 Size: 1 570 437 487 383 Fragments arranged by size: 570 487 437 383 1 BseYI C'CCAG_C Cuts at: 0 282 541 1255 1580 1878 Size: 282 259 714 325 298 Fragments arranged by size: 714 325 298 282 259 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 805 1878 Size: 805 1073 BsiHKAI G_wGCw'C Cuts at: 0 248 434 536 1445 1465 1846 1878 Size: 248 186 102 909 20 381 32 Fragments arranged by size: 909 381 248 186 102 32 20 BsmBI CGTCTCn'nnnn_ Cuts at: 0 372 1878 Size: 372 1506 Bsp1286I G_dGCh'C Cuts at: 0 248 405 434 476 536 548 841 1172 Size: 248 157 29 42 60 12 293 331 Cuts at: 1172 1184 1211 1238 1250 1277 1445 1465 1694 Size: 12 27 27 12 27 168 20 229 Cuts at: 1694 1846 1878 Size: 152 32 Fragments arranged by size: 331 293 248 229 168 157 152 60 42 32 29 27 27 27 20 12 12 12 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 140 1844 1878 Size: 140 1704 34 Fragments arranged by size: 1704 140 34 BsrBI CCG'CTC Cuts at: 0 1739 1878 Size: 1739 139 BsrDI GCAATG_nn' Cuts at: 0 453 1878 Size: 453 1425 BsrFI r'CCGG_y Cuts at: 0 377 1878 Size: 377 1501 BsrGI T'GTAC_A Cuts at: 0 176 1878 Size: 176 1702 BstAPI GCAn_nnn'nTGC Cuts at: 0 321 342 1878 Size: 321 21 1536 Fragments arranged by size: 1536 321 21 BstXI CCAn_nnnn'nTGG Cuts at: 0 265 1073 1659 1878 Size: 265 808 586 219 Fragments arranged by size: 808 586 265 219 BstYI r'GATC_y Cuts at: 0 294 683 1878 Size: 294 389 1195 Fragments arranged by size: 1195 389 294 BstZ17I GTA'TAC Cuts at: 0 602 1878 Size: 602 1276 BtgI C'CryG_G Cuts at: 0 654 1434 1878 Size: 654 780 444 Fragments arranged by size: 780 654 444 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1278 1878 Size: 1278 600 BtsI GCAGTG_nn' Cuts at: 0 340 793 989 1878 Size: 340 453 196 889 Fragments arranged by size: 889 453 340 196 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 911 946 1878 Size: 911 35 932 Fragments arranged by size: 932 911 35 EaeI y'GGCC_r Cuts at: 0 74 776 1617 1770 1866 1878 Size: 74 702 841 153 96 12 Fragments arranged by size: 841 702 153 96 74 12 EarI CTCTTCn'nnn_ Cuts at: 0 884 1540 1714 1753 1878 Size: 884 656 174 39 125 Fragments arranged by size: 884 656 174 125 39 EcoICRI GAG'CTC Cuts at: 0 246 432 534 1463 1878 Size: 246 186 102 929 415 Fragments arranged by size: 929 415 246 186 102 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 110 241 475 517 580 1225 1449 1681 Size: 110 131 234 42 63 645 224 232 Cuts at: 1681 1725 1878 Size: 44 153 Fragments arranged by size: 645 234 232 224 153 131 110 63 44 42 EcoO109I rG'GnC_Cy Cuts at: 0 287 626 1605 1780 1802 1833 1878 Size: 287 339 979 175 22 31 45 Fragments arranged by size: 979 339 287 175 45 31 22 HaeII r_GCGC'y Cuts at: 0 121 1878 Size: 121 1757 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 279 311 1806 1838 1878 Size: 279 32 1495 32 40 Fragments arranged by size: 1495 279 40 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1054 1878 Size: 1054 824 MscI TGG'CCA Cuts at: 0 76 778 1619 1868 1878 Size: 76 702 841 249 10 Fragments arranged by size: 841 702 249 76 10 MslI CAynn'nnrTG Cuts at: 0 161 1071 1315 1878 Size: 161 910 244 563 Fragments arranged by size: 910 563 244 161 MspA1I CmG'CkG Cuts at: 0 225 312 541 1878 Size: 225 87 229 1337 Fragments arranged by size: 1337 229 225 87 NspI r_CATG'y Cuts at: 0 1594 1878 Size: 1594 284 PasI CC'CwG_GG Cuts at: 0 469 965 1512 1878 Size: 469 496 547 366 Fragments arranged by size: 547 496 469 366 PfoI T'CCnGG_A Cuts at: 0 359 1175 1878 Size: 359 816 703 Fragments arranged by size: 816 703 359 PpuMI rG'GwC_Cy Cuts at: 0 287 626 1833 1878 Size: 287 339 1207 45 Fragments arranged by size: 1207 339 287 45 PshAI GACnn'nnGTC Cuts at: 0 931 1878 Size: 931 947 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 160 192 1878 Size: 160 32 1686 Fragments arranged by size: 1686 160 32 PstI C_TGCA'G Cuts at: 0 710 1670 1878 Size: 710 960 208 Fragments arranged by size: 960 710 208 PvuII CAG'CTG Cuts at: 0 225 312 1878 Size: 225 87 1566 Fragments arranged by size: 1566 225 87 SacI G_AGCT'C Cuts at: 0 248 434 536 1465 1878 Size: 248 186 102 929 413 Fragments arranged by size: 929 413 248 186 102 SanDI GG'GwC_CC Cuts at: 0 287 626 1878 Size: 287 339 1252 Fragments arranged by size: 1252 339 287 SapI GCTCTTCn'nnn_ Cuts at: 0 1714 1878 Size: 1714 164 SfcI C'TryA_G Cuts at: 0 706 1666 1878 Size: 706 960 212 Fragments arranged by size: 960 706 212 SmaI CCC'GGG Cuts at: 0 1696 1878 Size: 1696 182 SnaBI TAC'GTA Cuts at: 0 732 1878 Size: 732 1146 StyI C'CwwG_G Cuts at: 0 846 1446 1620 1878 Size: 846 600 174 258 Fragments arranged by size: 846 600 258 174 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 504 1878 Size: 504 1374 TatI w'GTAC_w Cuts at: 0 176 1878 Size: 176 1702 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 729 761 1087 1119 1415 1447 1667 1699 Size: 729 32 326 32 296 32 220 32 Cuts at: 1699 1878 Size: 179 Fragments arranged by size: 729 326 296 220 179 32 32 32 32 XbaI T'CTAG_A Cuts at: 0 999 1878 Size: 999 879 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 492 927 1878 Size: 492 435 951 Fragments arranged by size: 951 492 435 XmaI C'CCGG_G Cuts at: 0 1694 1878 Size: 1694 184 ZraI GAC'GTC Cuts at: 0 366 753 1878 Size: 366 387 1125 Fragments arranged by size: 1125 387 366 Enzymes that do cut: AatII AccI AcuI AfeI AleI AlfI AlwNI ApoI AvaI BanI BanII BbsI BbvCI BclI BglI BlpI Bme1580I BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI BstYI BstZ17I BtgI BtgZI BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I MmeI MscI MslI MspA1I NspI PasI PfoI PpuMI PshAI PsrI PstI PvuII SacI SanDI SapI SfcI SmaI SnaBI StyI TaqII TatI TstI XbaI XcmI XmaI ZraI Enzymes that do not cut: AarI Acc65I AclI AflII AflIII AgeI AhdI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BfrBI BglII BmgBI BmrI BmtI BpuEI BsaBI BsaWI BsiEI BsiWI BsmI BspEI BspHI BssHII BssSI BstBI BstEII Bsu36I ClaI DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PmeI PmlI PpiI PsiI PspOMI PspXI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SpeI SphI SrfI SspI StuI SwaI Tth111I XhoI XmnI