################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BsaBI BsaHI BsaI BseAI BseRI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I Cfr10I ClaI CpoI DraI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI Hin1I HincII HindII HpaI HphI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MroI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tru9I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AciI 687 CCGC CCGC(-3/-1) AspHI 34 GTGCTC G[AT]GC[AT]^C BanII 597 GAGCCC G[GA]GC[CT]^C BbrPI 432 CACGTG CAC^GTG BbsI 218 GAAGAC GAAGAC(2/6) BbvI 437 GCAGC GCAGC(8/12) BclI 182 TGATCA T^GATCA BcnI 522 CCCGG CC^[GC]GG BlpI 440 GCTCAGC GC^T[ACGT]AGC Bpu1102I 440 GCTCAGC GC^T[ACGT]AGC BpuAI 218 GAAGAC GAAGAC(2/6) BsaWI 326 TCCGGT [AT]^CCGG[AT] BsgI 435 GTGCAG GTGCAG(16/14) BsiHKAI 34 GTGCTC G[AT]GC[AT]^C BsmFI 71 GGGAC GGGAC(10/14) BssSI 712 CTCGTG CACGAG(-5/-1) CelII 440 GCTCAGC GC^T[ACGT]AGC CfoI 288 GCGC GCG^C DraII 540 GGGTCCT [GA]G^G[ACGT]CC[CT] DraIII 845 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 550 GACCTCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 695 CCGTGG C^C[GA][CT]GG EcoO109I 540 GGGTCCT [GA]G^G[ACGT]CC[CT] FbaI 182 TGATCA T^GATCA Fnu4HI 437 GCAGC GC^[ACGT]GC HaeII 287 GGCGCT [GA]GCGC^[CT] HhaI 288 GCGC GCG^C HinP1I 288 GCGC G^CGC HindIII 161 AAGCTT A^AGCTT ItaI 437 GCAGC GC^[ACGT]GC MboII 218 GAAGA GAAGA(8/7) MspA1I 175 CAGCTG C[AC]G^C[GT]G NciI 522 CCCGG CC^[GC]GG PmaCI 432 CACGTG CAC^GTG PmlI 432 CACGTG CAC^GTG PpuMI 540 GGGTCCT [GA]G^G[AT]CC[CT] PstI 386 CTGCAG CTGCA^G PvuII 175 CAGCTG CAG^CTG SfaNI 408 GATGC GCATC(5/9) SspI 421 AATATT AAT^ATT TaqI 103 TCGA T^CGA TseI 437 GCAGC G^C[AT]GC Tsp509I 742 AATT ^AATT TthHB8I 103 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### BalI 473 TGGCCA TGG^CCA 725 TGGCCA BpmI 343 CTGGAG CTGGAG(16/14) 801 CTGGAG BsaAI 198 CACGTA [CT]AC^GT[GA] 432 CACGTG BsmAI 238 GAGAC GTCTC(1/5) 559 GTCTC BstYI 127 GGATCT [GA]^GATC[CT] 566 GGATCT HapII 327 CCGG C^CGG 523 CCGG HgaI 271 GACGC GACGC(5/10) 672 GACGC HpaII 327 CCGG C^CGG 523 CCGG MaeII 199 ACGT A^CGT 433 ACGT MflI 127 GGATCT [GA]^GATC[CT] 566 GGATCT MluNI 473 TGGCCA TGG^CCA 725 TGGCCA MscI 473 TGGCCA TGG^CCA 725 TGGCCA MslI 505 CACCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 690 CATGGCCGTG MspI 327 CCGG C^CGG 523 CCGG PleI 649 GACTC GAGTC(4/5) 105 GAGTC XcmI 527 CCAACTTCTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 620 CCAGTAATGCCTTGG XhoII 127 GGATCT [GA]^GATC[CT] 566 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AfaI AluI AlwI AvaII BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EaeI EcoRII EcoT14I FokI HaeIII HinfI MaeIII MboI MnlI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfcI StyI TfiI Tsp45I TspRI