################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII AccII AccIII AclI AcyI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspI AviII AvrII BbeI BbvCI BcgI BciVI BclI BglI BglII BlnI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HindIII HinfI HpaI KasI KspI MamI MfeI MluI MroI MslI MunI MvnI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 547 GGTACC G^GTACC AccI 148 GTCTAC GT^[AC][GT]AC AflII 267 CTTAAG C^TTAAG Asp718I 547 GGTACC G^GTACC AvaI 1010 CCCGGG C^[CT]CG[GA]G BalI 26 TGGCCA TGG^CCA BamHI 1104 GGATCC G^GATCC BbrPI 1342 CACGTG CAC^GTG BbsI 408 GAAGAC GAAGAC(2/6) BfaI 1213 CTAG C^TAG BfrI 267 CTTAAG C^TTAAG BlpI 75 GCTCAGC GC^T[ACGT]AGC Bpu1102I 75 GCTCAGC GC^T[ACGT]AGC BpuAI 408 GAAGAC GAAGAC(2/6) BsaAI 1342 CACGTG [CT]AC^GT[GA] BsaI 11 GGTCTC GGTCTC(1/5) BsgI 820 GTGCAG GTGCAG(16/14) BsoBI 1010 CCCGGG C^[CT]CG[GA]G BsrDI 745 CATTGC GCAATG(2/0) BsrFI 511 GCCGGC [GA]^CCGG[CT] BstEII 997 GGTGACC G^GT[ACGT]ACC BstPI 997 GGTGACC G^GT[ACGT]ACC CelII 75 GCTCAGC GC^T[ACGT]AGC Cfr10I 511 GCCGGC [GA]^CCGG[CT] EcoO65I 997 GGTGACC G^GT[ACGT]ACC HaeII 1241 AGCGCC [GA]GCGC^[CT] HincII 970 GTCAAC GT[CT]^[GA]AC HindII 970 GTCAAC GT[CT]^[GA]AC KpnI 547 GGTACC GGTAC^C MaeI 1213 CTAG C^TAG MaeII 1343 ACGT A^CGT MluNI 26 TGGCCA TGG^CCA MscI 26 TGGCCA TGG^CCA MseI 268 TTAA T^TAA NaeI 511 GCCGGC GCC^GGC NcoI 5 CCATGG C^CATGG NgoMIV 511 GCCGGC G^CCGGC PmaCI 1342 CACGTG CAC^GTG PmlI 1342 CACGTG CAC^GTG SmaI 1010 CCCGGG CCC^GGG StuI 1119 AGGCCT AGG^CCT Tru9I 268 TTAA T^TAA XcmI 470 CCAGGCTGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1010 CCCGGG C^CCGGG XmaI 1010 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcsI 1068 AAATTC [GA]^AATT[CT] 1222 AAATTC AflIII 368 ACATGT A^C[GA][CT]GT 1277 ACATGT ApoI 1068 AAATTC [GA]^AATT[CT] 1222 AAATTC Asp700I 1218 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1405 GAAGGGGTTC AvaII 216 GGACC G^G[AT]CC 582 GGACC BsmAI 643 GAGAC GTCTC(1/5) 12 GTCTC BsmFI 1025 GTCCC GGGAC(10/14) 1041 GTCCC BspLU11I 368 ACATGT A^CATGT 1277 ACATGT BspMI 38 ACCTGC ACCTGC(4/8) 391 ACCTGC BstYI 713 AGATCC [GA]^GATC[CT] 1104 GGATCC CfoI 873 GCGC GCG^C 1242 GCGC EarI 909 CTCTTC CTCTTC(1/4) 1018 CTCTTC HhaI 873 GCGC GCG^C 1242 GCGC HinP1I 873 GCGC G^CGC 1242 GCGC Ksp632I 909 CTCTTC CTCTTC(1/4) 1018 CTCTTC MflI 713 AGATCC [GA]^GATC[CT] 1104 GGATCC PflMI 203 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 579 CCAGGACCTGG PpuMI 215 AGGACCC [GA]G^G[AT]CC[CT] 581 AGGACCT PshAI 217 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 367 GACATGTGTC PstI 348 CTGCAG CTGCA^G 475 CTGCAG SapI 908 GCTCTTC GCTCTTC(1/4) 1017 GCTCTTC TaqI 124 TCGA T^CGA 981 TCGA Tsp509I 1069 AATT ^AATT 1223 AATT TthHB8I 124 TCGA T^CGA 981 TCGA Van91I 203 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 579 CCAGGACCTGG XhoII 713 AGATCC [GA]^GATC[CT] 1104 GGATCC XmnI 1218 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1405 GAAGGGGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HpaII HphI ItaI MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TseI Tsp45I TspRI