Restriction map for
pFN21AE6249
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# NO CUT ENZYMES ( 192 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BclI BcnI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MaeI
MamI MboII MfeI MflI MluI
MluNI MroI MscI MslI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TaqI
TfiI Tsp45I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 23 enzymes) #
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AlwI 629 GGATC GGATC(4/5)
BanII 521 GAGCCC G[GA]GC[CT]^C
BciVI 60 GTATCC GTATCC(6/5)
BglI 406 GCCTCAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 521 GAGCCC G[AGT]GC[ACT]^C
BpmI 387 CTCCAG CTGGAG(16/14)
BsaAI 358 CACGTA [CT]AC^GT[GA]
BseRI 605 CTCCTC GAGGAG(10/8)
BsmAI 148 GTCTC GTCTC(1/5)
BsmI 192 GCATTC GAATGC(1/-1)
Bsp1286I 521 GAGCCC G[AGT]GC[ACT]^C
BsrDI 66 CATTGC GCAATG(2/0)
DdeI 660 CTCAG C^T[ACGT]AG
EcoRI 372 GAATTC G^AATTC
EcoT14I 509 CCAAGG C^C[AT][AT]GG
HaeIII 459 GGCC GG^CC
MaeII 359 ACGT A^CGT
MaeIII 622 GTAAC ^GT[ACGT]AC
MspA1I 667 CAGCTG C[AC]G^C[GT]G
PstI 472 CTGCAG CTGCA^G
PvuII 667 CAGCTG CAG^CTG
SspI 341 AATATT AAT^ATT
StyI 509 CCAAGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 681 CCGC CCGC(-3/-1)
738 CCGC
BsiYI 10 CCAGACTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
751 CCTCTGAAGGG
BslI 10 CCAGACTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
751 CCTCTGAAGGG
BsmFI 87 GTCCC GGGAC(10/14)
718 GTCCC
BssKI 567 CCTGG ^CC[ACGT]GG
586 CCTGG
BstNI 567 CCTGG CC^[AT]GG
586 CCTGG
Cac8I 53 GCAAGC GC[ACGT]^[ACGT]GC
669 GCTGGC
DpnII 230 GATC ^GATC
630 GATC
EcoRII 567 CCTGG ^CC[AT]GG
586 CCTGG
HinfI 335 GAGTC G^A[ACGT]TC
597 GACTC
HphI 454 GGTGA GGTGA(8/7)
706 GGTGA
MboI 230 GATC ^GATC
630 GATC
MseI 101 TTAA T^TAA
503 TTAA
MvaI 567 CCTGG CC^[AT]GG
586 CCTGG
NdeII 230 GATC ^GATC
630 GATC
PleI 597 GACTC GAGTC(4/5)
335 GAGTC
Sau3AI 230 GATC ^GATC
630 GATC
ScaI 383 AGTACT AGT^ACT
480 AGTACT
ScrFI 567 CCTGG CC^[ACGT]GG
586 CCTGG
SfaNI 487 GATGC GCATC(5/9)
647 GCATC
SfcI 264 CTACAG C^T[GA][CT]AG
472 CTGCAG
Tru9I 101 TTAA T^TAA
503 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AcsI AfaI AluI ApoI AvaII
BbvI BsaJI BsrI BstF5I Cfr13I
Fnu4HI FokI ItaI MnlI MwoI
NlaIII RsaI Sau96I TseI Tsp509I
TspRI