(Circular) (Six-base) MAPSORT of: pFN21AE3666.seq Check: 9230 from: 1 to: 5770 pFN21AE3666 5770 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2365 2365 Size: 5770 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3580 278 Size: 53 83 186 936 2044 2468 Fragments arranged by size: 2468 2044 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3113 1937 Size: 1176 4594 Acc65I G'GTAC_C Cuts at: 3091 3091 Size: 5770 AclI AA'CG_TT Cuts at: 1563 3897 4270 1563 Size: 2334 373 3063 Fragments arranged by size: 3063 2334 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2942 3833 5217 5485 1632 Size: 1310 891 1384 268 1917 Fragments arranged by size: 1917 1384 1310 891 268 AflII C'TTAA_G Cuts at: 820 1017 2111 820 Size: 197 1094 4479 Fragments arranged by size: 4479 1094 197 AflIII A'CryG_T Cuts at: 1203 2978 4669 5646 1203 Size: 1775 1691 977 1327 Fragments arranged by size: 1775 1691 1327 977 AgeI A'CCGG_T Cuts at: 4601 4601 Size: 5770 AhdI GACnn_n'nnGTC Cuts at: 4499 4499 Size: 5770 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5770 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5770 AlwNI CAG_nnn'CTG Cuts at: 2707 3513 5085 2707 Size: 806 1572 3392 Fragments arranged by size: 3392 1572 806 ApaLI G'TGCA_C Cuts at: 3827 4983 3827 Size: 1156 4614 ApoI r'AATT_y Cuts at: 1493 2788 3056 3079 3305 3457 5566 1493 Size: 1295 268 23 226 152 2109 1697 Fragments arranged by size: 2109 1697 1295 268 226 152 23 AseI AT'TA_AT Cuts at: 160 1032 4324 160 Size: 872 3292 1606 Fragments arranged by size: 3292 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 AvaI C'yCGr_G Cuts at: 1870 1943 3095 1870 Size: 73 1152 4545 Fragments arranged by size: 4545 1152 73 BamHI G'GATC_C Cuts at: 3100 3467 3100 Size: 367 5403 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3091 4546 618 Size: 325 217 627 1304 1455 1842 Fragments arranged by size: 1842 1455 1304 627 325 217 BanII G_rGCy'C Cuts at: 1391 3089 1391 Size: 1698 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2208 928 Size: 971 309 4490 Fragments arranged by size: 4490 971 309 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3961 3995 1903 Size: 34 2024 34 3678 Fragments arranged by size: 3678 2024 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2280 2577 3662 4872 2280 Size: 297 1085 1210 3178 Fragments arranged by size: 3178 1210 1085 297 BclI T'GATC_A Cuts at: 1548 2262 1548 Size: 714 5056 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4381 136 Size: 107 122 71 631 605 2709 1525 Fragments arranged by size: 2709 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5765 5765 Size: 5770 BlpI GC'TnA_GC Cuts at: 3187 3187 Size: 5770 Bme1580I G_kGCm'C Cuts at: 1790 2547 3831 4987 1790 Size: 757 1284 1156 2573 Fragments arranged by size: 2573 1284 1156 757 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5770 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3501 4459 442 Size: 416 53 83 399 24 2084 958 1753 Fragments arranged by size: 2084 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5770 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2350 4430 1239 Size: 1111 2080 2579 Fragments arranged by size: 2579 2080 1111 Bpu10I CC'TnA_GC Cuts at: 2282 2282 Size: 5770 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3895 4760 5058 3895 Size: 865 298 4607 Fragments arranged by size: 4607 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2359 4433 882 Size: 617 860 2074 2219 Fragments arranged by size: 2219 2074 860 617 BsaAI yAC'GTr Cuts at: 493 1204 3561 493 Size: 711 2357 2702 Fragments arranged by size: 2702 2357 711 BsaBI GATnn'nnATC Cuts at: 2940 5764 2940 Size: 2824 2946 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3577 3959 4594 275 Size: 53 83 186 936 2044 382 635 1451 Fragments arranged by size: 2044 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4203 4601 4875 5022 5433 4203 Size: 398 274 147 411 4540 Fragments arranged by size: 4540 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2027 2057 2339 2369 1178 Size: 30 165 30 624 30 282 30 4579 Fragments arranged by size: 4579 624 282 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2048 2449 1186 Size: 862 401 4507 Fragments arranged by size: 4507 862 401 BseYI C'CCAG_C Cuts at: 2125 4973 5469 2125 Size: 2848 496 2426 Fragments arranged by size: 2848 2426 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5197 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2624 3220 3981 5009 1540 Size: 275 186 623 596 761 1028 2301 Fragments arranged by size: 2301 1028 761 623 596 275 186 BsiHKAI G_wGCw'C Cuts at: 3831 3916 4987 3831 Size: 85 1071 4614 Fragments arranged by size: 4614 1071 85 BsmI GAATG_Cn' Cuts at: 3281 3374 3281 Size: 93 5677 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2547 3089 3831 3916 4987 1391 Size: 399 757 542 742 85 1071 2174 Fragments arranged by size: 2174 1071 757 742 542 399 85 BspEI T'CCGG_A Cuts at: 5433 5433 Size: 5770 BspHI T'CATG_A Cuts at: 3659 5389 3659 Size: 1730 4040 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2365 3125 844 Size: 1521 760 3489 Fragments arranged by size: 3489 1521 760 BsrBI CCG'CTC Cuts at: 1394 1823 3657 1394 Size: 429 1834 3507 Fragments arranged by size: 3507 1834 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4265 4439 65 Size: 1183 3017 174 1396 Fragments arranged by size: 3017 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2009 2552 4414 4601 1700 Size: 110 199 543 1862 187 2869 Fragments arranged by size: 2869 1862 543 199 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5770 BssHII G'CGCG_C Cuts at: 1925 2099 1925 Size: 174 5596 BssSI C'ACGA_G Cuts at: 3830 4842 3830 Size: 1012 4758 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5770 BstBI TT'CG_AA Cuts at: 4574 4574 Size: 5770 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5770 BstXI CCAn_nnnn'nTGG Cuts at: 2186 2186 Size: 5770 BstYI r'GATC_y Cuts at: 1972 2404 2821 3100 3467 3852 3869 5321 5407 Size: 432 417 279 367 385 17 1452 86 Cuts at: 5407 5419 5765 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 432 417 385 367 346 279 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3050 3050 Size: 5770 BtgI C'CryG_G Cuts at: 513 1065 2004 2028 2179 513 Size: 552 939 24 151 4104 Fragments arranged by size: 4104 939 552 151 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2285 2476 487 Size: 683 56 1059 191 3781 Fragments arranged by size: 3781 1059 683 191 56 BtsI GCAGTG_nn' Cuts at: 2644 3286 4080 4100 2644 Size: 642 794 20 4314 Fragments arranged by size: 4314 794 642 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5770 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5735 DraI TTT'AAA Cuts at: 2758 3074 2758 Size: 316 5454 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2079 4777 809 Size: 507 763 2698 1802 Fragments arranged by size: 2698 1802 763 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2614 3217 Size: 54 1425 50 275 18 177 607 603 Cuts at: 3217 4106 5735 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 607 603 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3217 1537 Size: 275 1405 4090 Fragments arranged by size: 4090 1405 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2733 2826 3700 1353 Size: 1380 93 874 3423 Fragments arranged by size: 3423 1380 874 93 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4357 4731 4877 387 Size: 1064 138 2768 374 146 1280 Fragments arranged by size: 2768 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2350 2942 3833 4430 5217 5485 1239 Size: 393 718 592 891 597 787 268 1524 Fragments arranged by size: 1524 891 787 718 597 592 393 268 EcoO109I rG'GnC_Cy Cuts at: 2704 2704 Size: 5770 EcoRI G'AATT_C Cuts at: 2788 3056 3079 3457 2788 Size: 268 23 378 5101 Fragments arranged by size: 5101 378 268 23 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2546 2546 Size: 5770 FspI TGC'GCA Cuts at: 4276 4276 Size: 5770 HaeII r_GCGC'y Cuts at: 1678 4917 1678 Size: 3239 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2027 2059 2339 2371 2901 Size: 32 135 32 455 32 280 32 530 Cuts at: 2901 2933 4411 4443 4485 4517 1373 Size: 32 1478 32 42 32 2626 Fragments arranged by size: 2626 1478 530 455 280 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3114 3360 669 Size: 988 281 1176 246 3079 Fragments arranged by size: 3079 1176 988 281 246 HindIII A'AGCT_T Cuts at: 748 2924 748 Size: 2176 3594 HpaI GTT'AAC Cuts at: 3360 3360 Size: 5770 KpnI G_GTAC'C Cuts at: 3095 3095 Size: 5770 MfeI C'AATT_G Cuts at: 3369 3369 Size: 5770 MluI A'CGCG_T Cuts at: 5646 5646 Size: 5770 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2433 4884 5068 1305 Size: 1128 2451 184 2007 Fragments arranged by size: 2451 2007 1128 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2616 10 Size: 54 1425 343 784 3164 Fragments arranged by size: 3164 1425 784 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2249 3728 4087 4246 518 Size: 648 1083 1479 359 159 2042 Fragments arranged by size: 2042 1479 1083 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2030 2449 3186 3863 5011 5256 1606 Size: 424 419 737 677 1148 245 2120 Fragments arranged by size: 2120 1148 737 677 424 419 245 NaeI GCC'GGC Cuts at: 2011 2011 Size: 5770 NcoI C'CATG_G Cuts at: 513 1065 2004 2179 513 Size: 552 939 175 4104 Fragments arranged by size: 4104 939 552 175 NdeI CA'TA_TG Cuts at: 387 387 Size: 5770 NgoMIV G'CCGG_C Cuts at: 2009 2009 Size: 5770 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5770 NotI GC'GGCC_GC Cuts at: 3217 3217 Size: 5770 NspI r_CATG'y Cuts at: 1132 1229 2982 3128 4673 1132 Size: 97 1753 146 1545 2229 Fragments arranged by size: 2229 1753 1545 146 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5770 PciI A'CATG_T Cuts at: 2978 4669 2978 Size: 1691 4079 PflMI CCAn_nnn'nTGG Cuts at: 1108 2707 1108 Size: 1599 4171 PfoI T'CCnGG_A Cuts at: 2415 2415 Size: 5770 PmeI GTTT'AAAC Cuts at: 3074 3074 Size: 5770 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3839 3871 5352 5384 3839 Size: 32 1481 32 4225 Fragments arranged by size: 4225 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2704 2704 Size: 5770 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5770 PsiI TTA'TAA Cuts at: 3340 3340 Size: 5770 PstI C_TGCA'G Cuts at: 830 3122 830 Size: 2292 3478 PvuI CG_AT'CG Cuts at: 2001 2624 2001 Size: 623 5147 PvuII CAG'CTG Cuts at: 2449 2449 Size: 5770 SacII CC_GC'GG Cuts at: 2031 2031 Size: 5770 SalI G'TCGA_C Cuts at: 1936 3112 1936 Size: 1176 4594 SbfI CC_TGCA'GG Cuts at: 3122 3122 Size: 5770 ScaI AGT'ACT Cuts at: 4018 5730 4018 Size: 1712 4058 SexAI A'CCwGG_T Cuts at: 2700 2700 Size: 5770 SfcI C'TryA_G Cuts at: 826 1045 2985 3038 3118 4253 4934 5125 826 Size: 219 1940 53 80 1135 681 191 1471 Fragments arranged by size: 1940 1471 1135 681 219 191 80 53 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5770 SmaI CCC'GGG Cuts at: 1872 3097 1872 Size: 1225 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2111 3874 4775 5037 820 Size: 197 926 168 1763 901 262 1553 Fragments arranged by size: 1763 1553 926 901 262 197 168 SnaBI TAC'GTA Cuts at: 493 3561 493 Size: 3068 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5770 SphI G_CATG'C Cuts at: 1132 3128 1132 Size: 1996 3774 SspI AAT'ATT Cuts at: 5 52 2468 3001 3694 5 Size: 47 2416 533 693 2081 Fragments arranged by size: 2416 2081 693 533 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5770 StyI C'CwwG_G Cuts at: 513 1065 2004 2179 3209 513 Size: 552 939 175 1030 3074 Fragments arranged by size: 3074 1030 939 552 175 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2899 3797 3814 3967 4152 4597 325 Size: 1255 1319 898 17 153 185 445 1498 Fragments arranged by size: 1498 1319 1255 898 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2844 4016 5728 Size: 274 80 33 51 1443 867 1172 1712 Cuts at: 5728 96 Size: 138 Fragments arranged by size: 1712 1443 1172 867 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5730 5762 5730 Size: 32 5738 Tth111I GACn'n_nGTC Cuts at: 1316 2351 2702 1316 Size: 1035 351 4384 Fragments arranged by size: 4384 1035 351 XbaI T'CTAG_A Cuts at: 3106 3106 Size: 5770 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2374 2674 2374 Size: 300 5470 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5770 XmaI C'CCGG_G Cuts at: 1870 3095 1870 Size: 1225 4545 XmnI GAAnn'nnTTC Cuts at: 3899 3899 Size: 5770 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3578 276 Size: 53 83 186 936 2044 2468 Fragments arranged by size: 2468 2044 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI