################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII Acc65I AccI AclI AcyI Alw44I AocI Aor51HI ApaLI AscI Asp700I Asp718I AvrII BamHI BbeI BbrPI BciVI BglI BglII BlnI BsaBI BsaHI BsgI BsiEI BsiWI BspDI BspLU11I BspMI BsrBI BsrDI BssHII Bst1107I BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI Hin1I KasI KpnI KspI MamI MfeI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SgrAI SmaI SnaBI Sse8387I SspI StuI SwaI TfiI Van91I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### AflIII 161 ACGCGT A^C[GA][CT]GT AgeI 1876 ACCGGT A^CCGGT AhdI 1446 GACAGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 328 ATTAAT AT^TAAT AsnI 328 ATTAAT AT^TAAT AspEI 1446 GACAGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1277 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1774 CTCGAG C^[CT]CG[GA]G AviII 1499 TGCGCA TGC^GCA BalI 1073 TGGCCA TGG^CCA BanI 1303 GGTGCC G^G[CT][GA]CC BbsI 598 GAAGAC GAAGAC(2/6) BbvCI 1543 CCTCAGC CCTCAGC(-5/-2) BcgI 296 GCAACATATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 137 TGATCA T^GATCA BcnI 96 CCCGG CC^[GC]GG BlpI 1102 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1102 GCTCAGC GC^T[ACGT]AGC BpuAI 598 GAAGAC GAAGAC(2/6) BsaAI 40 TACGTG [CT]AC^GT[GA] BsmBI 925 CGTCTC CGTCTC(1/5) BsmI 479 GAATGC GAATGC(1/-1) BsoBI 1774 CTCGAG C^[CT]CG[GA]G BspHI 1208 TCATGA T^CATGA BsrFI 1876 ACCGGT [GA]^CCGG[CT] BssSI 1403 CACGAG CACGAG(-5/-1) BstAPI 1465 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 278 TTCGAA TT^CGAA CelII 1102 GCTCAGC GC^T[ACGT]AGC Cfr10I 1876 ACCGGT [GA]^CCGG[CT] DsaI 1510 CCACGG C^C[GA][CT]GG EaeI 1073 TGGCCA [CT]^GGCC[GA] Eam1105I 1446 GACAGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1114 GAAGAG CTCTTC(1/4) EcoNI 1192 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 410 CCAAGG C^C[AT][AT]GG FbaI 137 TGATCA T^GATCA FspI 1499 TGCGCA TGC^GCA HgaI 190 GACGC GACGC(5/10) HindIII 708 AAGCTT A^AGCTT HpaI 1864 GTTAAC GTT^AAC Ksp632I 1114 GAAGAG CTCTTC(1/4) MluI 161 ACGCGT A^CGCGT MluNI 1073 TGGCCA TGG^CCA MscI 1073 TGGCCA TGG^CCA NciI 96 CCCGG CC^[GC]GG NspV 278 TTCGAA TT^CGAA PaeR7I 1774 CTCGAG C^TCGAG PflFI 1277 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1876 ACCGGT A^CCGGT PpuMI 47 GGGACCT [GA]G^G[AT]CC[CT] PshAI 1212 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 328 ATTAAT AT^TAAT RcaI 1208 TCATGA T^CATGA SapI 1114 GAAGAGC GCTCTTC(1/4) ScaI 1372 AGTACT AGT^ACT SexAI 872 ACCAGGT A^CC[AT]GGT SfuI 278 TTCGAA TT^CGAA SpeI 961 ACTAGT A^CTAGT SphI 198 GCATGC GCATG^C StyI 410 CCAAGG C^C[AT][AT]GG Tth111I 1277 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1279 CCAAGTCCTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1774 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccII 162 CGCG CG^CG 192 CGCG AccIII 119 TCCGGA T^CCGGA 929 TCCGGA AlwNI 353 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG 1466 CAGCTGCTG ApaI 103 GGGCCC GGGCC^C 643 GGGCCC AvaII 48 GGACC G^G[AT]CC 1453 GGTCC BpmI 1626 CTCCAG CTGGAG(16/14) 1781 CTCCAG BsaI 1380 GAGACC GGTCTC(1/5) 257 GGTCTC BseAI 119 TCCGGA T^CCGGA 929 TCCGGA BsiYI 1192 CCTCCTTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1794 CCGTTCTAAGG BslI 1192 CCTCCTTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1794 CCGTTCTAAGG Bsp1407I 426 TGTACA T^GTACA 1599 TGTACA BspEI 119 TCCGGA T^CCGGA 929 TCCGGA BsrGI 426 TGTACA T^GTACA 1599 TGTACA BstUI 162 CGCG CG^CG 192 CGCG BstYI 122 GGATCT [GA]^GATC[CT] 624 GGATCT DraII 47 GGGACCT [GA]G^G[ACGT]CC[CT] 102 AGGGCCC EcoO109I 47 GGGACCT [GA]G^G[ACGT]CC[CT] 102 AGGGCCC HaeII 614 AGCGCC [GA]GCGC^[CT] 1118 AGCGCC HincII 1413 GTCAAC GT[CT]^[GA]AC 1864 GTTAAC HindII 1413 GTCAAC GT[CT]^[GA]AC 1864 GTTAAC MflI 122 GGATCT [GA]^GATC[CT] 624 GGATCT MroI 119 TCCGGA T^CCGGA 929 TCCGGA MvnI 162 CGCG CG^CG 192 CGCG NspI 198 GCATGC [GA]CATG^[CT] 1733 GCATGT SacI 763 GAGCTC GAGCT^C 794 GAGCTC SspBI 426 TGTACA T^GTACA 1599 TGTACA Tsp45I 64 GTGAC ^GT[GC]AC 1788 GTCAC XhoII 122 GGATCT [GA]^GATC[CT] 624 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AciI AcsI AfaI AflII AluI AlwI ApoI AspHI BanII BbvI BfaI BfrI BmyI BsaJI BsaWI BseRI BsiHKAI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI Tru9I TseI Tsp509I TspRI TthHB8I