(Circular) (Six-base) MAPSORT of: pFN21AE5715.seq Check: 9194 from: 1 to: 6598 pFN21AE5715 6598 bp With 182 enzymes: SgfI * December 8, 2014 13:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4408 278 Size: 53 83 186 936 2872 2468 Fragments arranged by size: 2872 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3941 1937 Size: 2004 4594 Acc65I G'GTAC_C Cuts at: 3919 3919 Size: 6598 AclI AA'CG_TT Cuts at: 1563 4725 5098 1563 Size: 3162 373 3063 Fragments arranged by size: 3162 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3186 4661 6045 6313 1632 Size: 1554 1475 1384 268 1917 Fragments arranged by size: 1917 1554 1475 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2624 2656 2831 820 Size: 197 1607 32 175 4587 Fragments arranged by size: 4587 1607 197 175 32 AflIII A'CryG_T Cuts at: 1203 2166 5497 6474 1203 Size: 963 3331 977 1327 Fragments arranged by size: 3331 1327 977 963 AgeI A'CCGG_T Cuts at: 3881 5429 3881 Size: 1548 5050 AhdI GACnn_n'nnGTC Cuts at: 3456 5327 3456 Size: 1871 4727 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6598 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2748 3447 3609 1184 Size: 1564 699 162 4173 Fragments arranged by size: 4173 1564 699 162 AlwNI CAG_nnn'CTG Cuts at: 2363 3476 4341 5913 2363 Size: 1113 865 1572 3048 Fragments arranged by size: 3048 1572 1113 865 ApaI G_GGCC'C Cuts at: 2112 2652 2112 Size: 540 6058 ApaLI G'TGCA_C Cuts at: 4655 5811 4655 Size: 1156 5442 ApoI r'AATT_y Cuts at: 1493 2221 2593 2784 3907 4133 4285 6394 1493 Size: 728 372 191 1123 226 152 2109 1697 Fragments arranged by size: 2109 1697 1123 728 372 226 191 152 AseI AT'TA_AT Cuts at: 160 1032 2334 5152 160 Size: 872 1302 2818 1606 Fragments arranged by size: 2818 1606 1302 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6598 AvaI C'yCGr_G Cuts at: 1870 1943 3779 3923 1870 Size: 73 1836 144 4545 Fragments arranged by size: 4545 1836 144 73 BamHI G'GATC_C Cuts at: 3928 4295 3928 Size: 367 6231 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3308 3919 5374 618 Size: 325 217 627 1521 611 1455 1842 Fragments arranged by size: 1842 1521 1455 627 611 325 217 BanII G_rGCy'C Cuts at: 1391 2112 2652 2772 2803 3917 1391 Size: 721 540 120 31 1114 4072 Fragments arranged by size: 4072 1114 721 540 120 31 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2610 928 Size: 971 711 4916 Fragments arranged by size: 4916 971 711 BbvCI CC'TCA_GC Cuts at: 3549 3549 Size: 6598 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2290 2324 4789 4823 1903 Size: 34 353 34 2465 34 3678 Fragments arranged by size: 3678 2465 353 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4490 5700 4490 Size: 1210 5388 BclI T'GATC_A Cuts at: 1548 2142 1548 Size: 594 6004 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5209 136 Size: 107 122 71 631 605 3537 1525 Fragments arranged by size: 3537 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6593 6593 Size: 6598 BlpI GC'TnA_GC Cuts at: 3108 4015 3108 Size: 907 5691 Bme1580I G_kGCm'C Cuts at: 1790 2112 2652 3183 4659 5815 1790 Size: 322 540 531 1476 1156 2573 Fragments arranged by size: 2573 1476 1156 540 531 322 BmgBI CAC'GTC Cuts at: 1318 2068 3464 1318 Size: 750 1396 4452 Fragments arranged by size: 4452 1396 750 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3333 4329 5287 Size: 416 53 83 399 24 1916 996 958 Cuts at: 5287 442 Size: 1753 Fragments arranged by size: 1916 1753 996 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6598 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3616 3771 5258 1239 Size: 2377 155 1487 2579 Fragments arranged by size: 2579 2377 1487 155 Bpu10I CC'TnA_GC Cuts at: 3303 3549 3303 Size: 246 6352 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4723 5588 5886 4723 Size: 865 298 5435 Fragments arranged by size: 5435 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2268 3379 5261 882 Size: 617 769 1111 1882 2219 Fragments arranged by size: 2219 1882 1111 769 617 BsaAI yAC'GTr Cuts at: 493 1204 2047 4389 493 Size: 711 843 2342 2702 Fragments arranged by size: 2702 2342 843 711 BsaBI GATnn'nnATC Cuts at: 6592 6592 Size: 6598 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4405 4787 5422 275 Size: 53 83 186 936 2872 382 635 1451 Fragments arranged by size: 2872 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2124 2934 3881 5031 5429 5703 5850 6261 2124 Size: 810 947 1150 398 274 147 411 2461 Fragments arranged by size: 2461 1150 947 810 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6373 Fragments arranged by size: 6373 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2146 3016 3642 1186 Size: 960 870 626 4142 Fragments arranged by size: 4142 960 870 626 BseYI C'CCAG_C Cuts at: 2012 2021 3722 5801 6297 2012 Size: 9 1701 2079 496 2313 Fragments arranged by size: 2313 2079 1701 496 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6025 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4048 4809 5837 1540 Size: 275 186 2047 761 1028 2301 Fragments arranged by size: 2301 2047 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2772 2803 3196 3361 4659 4744 5815 2772 Size: 31 393 165 1298 85 1071 3555 Fragments arranged by size: 3555 1298 1071 393 165 85 31 BsmI GAATG_Cn' Cuts at: 2490 4109 4202 2490 Size: 1619 93 4886 Fragments arranged by size: 4886 1619 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2936 2936 Size: 6598 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2112 2652 2772 2803 3183 3196 3361 Size: 399 322 540 120 31 380 13 165 Cuts at: 3361 3917 4659 4744 5815 1391 Size: 556 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 556 540 399 380 322 165 120 85 31 13 BspEI T'CCGG_A Cuts at: 2124 2934 6261 2124 Size: 810 3327 2461 Fragments arranged by size: 3327 2461 810 BspHI T'CATG_A Cuts at: 3213 4487 6217 3213 Size: 1274 1730 3594 Fragments arranged by size: 3594 1730 1274 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3953 844 Size: 3109 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4485 1394 Size: 429 2662 3507 Fragments arranged by size: 3507 2662 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5093 5267 65 Size: 1183 3845 174 1396 Fragments arranged by size: 3845 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3881 5242 5429 1700 Size: 110 2071 1361 187 2869 Fragments arranged by size: 2869 2071 1361 187 110 BsrGI T'GTAC_A Cuts at: 96 2431 3604 96 Size: 2335 1173 3090 Fragments arranged by size: 3090 2335 1173 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6598 BssSI C'ACGA_G Cuts at: 3408 4658 5670 3408 Size: 1250 1012 4336 Fragments arranged by size: 4336 1250 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3476 794 Size: 2682 3916 BstBI TT'CG_AA Cuts at: 2284 5402 2284 Size: 3118 3480 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6598 BstYI r'GATC_y Cuts at: 1972 2127 2629 3928 4295 4680 4697 6149 6235 Size: 155 502 1299 367 385 17 1452 86 Cuts at: 6235 6247 6593 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1299 502 385 367 346 155 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3515 513 Size: 552 2450 3596 Fragments arranged by size: 3596 2450 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2093 487 Size: 683 56 867 4992 Fragments arranged by size: 4992 867 683 56 BtsI GCAGTG_nn' Cuts at: 4114 4908 4928 4114 Size: 794 20 5784 Fragments arranged by size: 5784 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6598 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6563 DraI TTT'AAA Cuts at: 3902 3902 Size: 6598 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5605 809 Size: 507 4289 1802 Fragments arranged by size: 4289 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3078 4045 4934 Size: 54 1425 50 275 18 1248 967 889 Cuts at: 4934 6563 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1248 967 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4045 1537 Size: 275 2233 4090 Fragments arranged by size: 4090 2233 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3114 4528 1353 Size: 1761 1414 3423 Fragments arranged by size: 3423 1761 1414 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3338 5185 5559 5705 387 Size: 1064 138 1749 1847 374 146 1280 Fragments arranged by size: 1847 1749 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2770 2801 2770 Size: 31 6567 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3186 3616 3771 4661 5258 6045 6313 Size: 393 1554 430 155 890 597 787 268 Cuts at: 6313 1239 Size: 1524 Fragments arranged by size: 1554 1524 890 787 597 430 393 268 155 EcoNI CCTnn'n_nnAGG Cuts at: 3201 3201 Size: 6598 EcoO109I rG'GnC_Cy Cuts at: 2053 2108 2053 Size: 55 6543 EcoRI G'AATT_C Cuts at: 3907 4285 3907 Size: 378 6220 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2567 2650 2567 Size: 83 6515 FspI TGC'GCA Cuts at: 3506 5104 3506 Size: 1598 5000 FspAI rTGC'GCAy Cuts at: 3506 3506 Size: 6598 HaeII r_GCGC'y Cuts at: 1678 2623 3127 5745 1678 Size: 945 504 2618 2531 Fragments arranged by size: 2618 2531 945 504 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3442 3474 5239 5271 5313 Size: 32 135 32 1870 32 1765 32 42 Cuts at: 5313 5345 1373 Size: 32 2626 Fragments arranged by size: 2626 1870 1765 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3420 3871 3942 4188 669 Size: 988 281 1482 451 71 246 3079 Fragments arranged by size: 3079 1482 988 451 281 246 71 HindIII A'AGCT_T Cuts at: 748 2713 748 Size: 1965 4633 HpaI GTT'AAC Cuts at: 3871 4188 3871 Size: 317 6281 KpnI G_GTAC'C Cuts at: 3923 3923 Size: 6598 MfeI C'AATT_G Cuts at: 4197 4197 Size: 6598 MluI A'CGCG_T Cuts at: 2166 6474 2166 Size: 4308 2290 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2184 2494 3726 3914 5712 5896 1305 Size: 879 310 1232 188 1798 184 2007 Fragments arranged by size: 2007 1798 1232 879 310 188 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3080 10 Size: 54 1425 343 1248 3528 Fragments arranged by size: 3528 1425 1248 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2730 3561 3737 4556 4915 5074 518 Size: 648 1564 831 176 819 359 159 2042 Fragments arranged by size: 2042 1564 831 819 648 359 176 159 MspA1I CmG'CkG Cuts at: 1606 2151 2456 3221 3473 4014 4691 5839 6084 Size: 545 305 765 252 541 677 1148 245 Cuts at: 6084 1606 Size: 2120 Fragments arranged by size: 2120 1148 765 677 545 541 305 252 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6046 NdeI CA'TA_TG Cuts at: 387 387 Size: 6598 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6598 NotI GC'GGCC_GC Cuts at: 4045 4045 Size: 6598 NspI r_CATG'y Cuts at: 1132 1229 2207 3742 3956 5501 1132 Size: 97 978 1535 214 1545 2229 Fragments arranged by size: 2229 1545 1535 978 214 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6598 PciI A'CATG_T Cuts at: 5497 5497 Size: 6598 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6598 PmeI GTTT'AAAC Cuts at: 3902 3902 Size: 6598 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4667 4699 6180 6212 4667 Size: 32 1481 32 5053 Fragments arranged by size: 5053 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2053 2053 Size: 6598 PshAI GACnn'nnGTC Cuts at: 1613 3221 1613 Size: 1608 4990 PsiI TTA'TAA Cuts at: 2234 4168 2234 Size: 1934 4664 PspOMI G'GGCC_C Cuts at: 2108 2648 2108 Size: 540 6058 PspXI vC'TCGA_Gb Cuts at: 3779 3779 Size: 6598 PstI C_TGCA'G Cuts at: 830 2135 3472 3490 3950 830 Size: 1305 1337 18 460 3478 Fragments arranged by size: 3478 1337 1305 460 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6598 PvuII CAG'CTG Cuts at: 2456 3221 3473 2456 Size: 765 252 5581 Fragments arranged by size: 5581 765 252 SacI G_AGCT'C Cuts at: 2772 2803 2772 Size: 31 6567 SalI G'TCGA_C Cuts at: 1936 3940 1936 Size: 2004 4594 SapI GCTCTTCn'nnn_ Cuts at: 3114 3114 Size: 6598 SbfI CC_TGCA'GG Cuts at: 3950 3950 Size: 6598 ScaI AGT'ACT Cuts at: 3379 4846 6558 3379 Size: 1467 1712 3419 Fragments arranged by size: 3419 1712 1467 SexAI A'CCwGG_T Cuts at: 2877 2877 Size: 6598 SfcI C'TryA_G Cuts at: 826 1045 2131 2745 3468 3486 3819 3946 5081 Size: 219 1086 614 723 18 333 127 1135 Cuts at: 5081 5762 5953 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1086 723 681 614 333 219 191 127 18 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6598 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6598 SmaI CCC'GGG Cuts at: 1872 3925 1872 Size: 2053 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2624 2656 2831 3779 4702 5603 Size: 197 926 681 32 175 948 923 901 Cuts at: 5603 5865 820 Size: 262 1553 Fragments arranged by size: 1553 948 926 923 901 681 262 197 175 32 SnaBI TAC'GTA Cuts at: 493 4389 493 Size: 3896 2702 SpeI A'CTAG_T Cuts at: 152 2966 152 Size: 2814 3784 SphI G_CATG'C Cuts at: 1132 2207 3956 1132 Size: 1075 1749 3774 Fragments arranged by size: 3774 1749 1075 SspI AAT'ATT Cuts at: 5 52 4522 5 Size: 47 4470 2081 Fragments arranged by size: 4470 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6598 StyI C'CwwG_G Cuts at: 513 1065 2415 4037 513 Size: 552 1350 1622 3074 Fragments arranged by size: 3074 1622 1350 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2690 4625 4642 4795 4980 5425 325 Size: 1255 1110 1935 17 153 185 445 1498 Fragments arranged by size: 1935 1498 1255 1110 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2431 3377 3604 Size: 274 80 33 51 1443 454 946 227 Cuts at: 3604 3826 4844 6556 96 Size: 222 1018 1712 138 Fragments arranged by size: 1712 1443 1018 946 454 274 227 222 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6558 6590 6558 Size: 32 6566 Tth111I GACn'n_nGTC Cuts at: 1316 3285 1316 Size: 1969 4629 XbaI T'CTAG_A Cuts at: 3934 3934 Size: 6598 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3291 3291 Size: 6598 XhoI C'TCGA_G Cuts at: 1943 3779 1943 Size: 1836 4762 XmaI C'CCGG_G Cuts at: 1870 3923 1870 Size: 2053 4545 XmnI GAAnn'nnTTC Cuts at: 4727 4727 Size: 6598 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4406 276 Size: 53 83 186 936 2872 2468 Fragments arranged by size: 2872 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI