(Linear) (Six-base) MAPSORT of: KIEE5715.seq Check: 4497 from: 1 to: 1893 KIEE5715.orf With 182 enzymes: SgfI * December 5, 2014 13:07 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1181 1893 Size: 1181 712 AflII C'TTAA_G Cuts at: 0 619 651 826 1893 Size: 619 32 175 1067 Fragments arranged by size: 1067 619 175 32 AflIII A'CryG_T Cuts at: 0 161 1893 Size: 161 1732 AgeI A'CCGG_T Cuts at: 0 1876 1893 Size: 1876 17 AhdI GACnn_n'nnGTC Cuts at: 0 1451 1893 Size: 1451 442 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 743 1442 1604 1893 Size: 743 699 162 289 Fragments arranged by size: 743 699 289 162 AlwNI CAG_nnn'CTG Cuts at: 0 358 1471 1893 Size: 358 1113 422 Fragments arranged by size: 1113 422 358 ApaI G_GGCC'C Cuts at: 0 107 647 1893 Size: 107 540 1246 Fragments arranged by size: 1246 540 107 ApoI r'AATT_y Cuts at: 0 216 588 779 1893 Size: 216 372 191 1114 Fragments arranged by size: 1114 372 216 191 AseI AT'TA_AT Cuts at: 0 329 1893 Size: 329 1564 AvaI C'yCGr_G Cuts at: 0 1774 1893 Size: 1774 119 BanI G'GyrC_C Cuts at: 0 1303 1893 Size: 1303 590 BanII G_rGCy'C Cuts at: 0 107 647 767 798 1893 Size: 107 540 120 31 1095 Fragments arranged by size: 1095 540 120 107 31 BbsI GAAGACnn'nnnn_ Cuts at: 0 605 1893 Size: 605 1288 BbvCI CC'TCA_GC Cuts at: 0 1544 1893 Size: 1544 349 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 285 319 1893 Size: 285 34 1574 Fragments arranged by size: 1574 285 34 BclI T'GATC_A Cuts at: 0 137 1893 Size: 137 1756 BlpI GC'TnA_GC Cuts at: 0 1103 1893 Size: 1103 790 Bme1580I G_kGCm'C Cuts at: 0 107 647 1178 1893 Size: 107 540 531 715 Fragments arranged by size: 715 540 531 107 BmgBI CAC'GTC Cuts at: 0 63 1459 1893 Size: 63 1396 434 Fragments arranged by size: 1396 434 63 BmrI ACTGGGnnnn_n' Cuts at: 0 1328 1893 Size: 1328 565 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1611 1766 1893 Size: 1611 155 127 Fragments arranged by size: 1611 155 127 Bpu10I CC'TnA_GC Cuts at: 0 1298 1544 1893 Size: 1298 246 349 Fragments arranged by size: 1298 349 246 BsaI GGTCTCn'nnnn_ Cuts at: 0 263 1374 1893 Size: 263 1111 519 Fragments arranged by size: 1111 519 263 BsaAI yAC'GTr Cuts at: 0 42 1893 Size: 42 1851 BsaWI w'CCGG_w Cuts at: 0 119 929 1876 1893 Size: 119 810 947 17 Fragments arranged by size: 947 810 119 17 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 141 1011 1637 1893 Size: 141 870 626 256 Fragments arranged by size: 870 626 256 141 BseYI C'CCAG_C Cuts at: 0 7 16 1717 1893 Size: 7 9 1701 176 Fragments arranged by size: 1701 176 9 7 BsiHKAI G_wGCw'C Cuts at: 0 767 798 1191 1356 1893 Size: 767 31 393 165 537 Fragments arranged by size: 767 537 393 165 31 BsmI GAATG_Cn' Cuts at: 0 485 1893 Size: 485 1408 BsmBI CGTCTCn'nnnn_ Cuts at: 0 931 1893 Size: 931 962 Bsp1286I G_dGCh'C Cuts at: 0 107 647 767 798 1178 1191 1356 1893 Size: 107 540 120 31 380 13 165 537 Fragments arranged by size: 540 537 380 165 120 107 31 13 BspEI T'CCGG_A Cuts at: 0 119 929 1893 Size: 119 810 964 Fragments arranged by size: 964 810 119 BspHI T'CATG_A Cuts at: 0 1208 1893 Size: 1208 685 BsrFI r'CCGG_y Cuts at: 0 1876 1893 Size: 1876 17 BsrGI T'GTAC_A Cuts at: 0 426 1599 1893 Size: 426 1173 294 Fragments arranged by size: 1173 426 294 BssSI C'ACGA_G Cuts at: 0 1403 1893 Size: 1403 490 BstAPI GCAn_nnn'nTGC Cuts at: 0 1471 1893 Size: 1471 422 BstBI TT'CG_AA Cuts at: 0 279 1893 Size: 279 1614 BstYI r'GATC_y Cuts at: 0 122 624 1893 Size: 122 502 1269 Fragments arranged by size: 1269 502 122 BtgI C'CryG_G Cuts at: 0 1510 1893 Size: 1510 383 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 88 1893 Size: 88 1805 EaeI y'GGCC_r Cuts at: 0 1073 1893 Size: 1073 820 EarI CTCTTCn'nnn_ Cuts at: 0 1109 1893 Size: 1109 784 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1333 1893 Size: 1333 560 EcoICRI GAG'CTC Cuts at: 0 765 796 1893 Size: 765 31 1097 Fragments arranged by size: 1097 765 31 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1181 1611 1766 1893 Size: 1181 430 155 127 Fragments arranged by size: 1181 430 155 127 EcoNI CCTnn'n_nnAGG Cuts at: 0 1196 1893 Size: 1196 697 EcoO109I rG'GnC_Cy Cuts at: 0 48 103 1893 Size: 48 55 1790 Fragments arranged by size: 1790 55 48 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 562 645 1893 Size: 562 83 1248 Fragments arranged by size: 1248 562 83 FspI TGC'GCA Cuts at: 0 1501 1893 Size: 1501 392 FspAI rTGC'GCAy Cuts at: 0 1501 1893 Size: 1501 392 HaeII r_GCGC'y Cuts at: 0 618 1122 1893 Size: 618 504 771 Fragments arranged by size: 771 618 504 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1437 1469 1893 Size: 1437 32 424 Fragments arranged by size: 1437 424 32 HincII GTy'rAC Cuts at: 0 1415 1866 1893 Size: 1415 451 27 Fragments arranged by size: 1415 451 27 HindIII A'AGCT_T Cuts at: 0 708 1893 Size: 708 1185 HpaI GTT'AAC Cuts at: 0 1866 1893 Size: 1866 27 MluI A'CGCG_T Cuts at: 0 161 1893 Size: 161 1732 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 179 489 1721 1893 Size: 179 310 1232 172 Fragments arranged by size: 1232 310 179 172 MscI TGG'CCA Cuts at: 0 1075 1893 Size: 1075 818 MslI CAynn'nnrTG Cuts at: 0 725 1556 1732 1893 Size: 725 831 176 161 Fragments arranged by size: 831 725 176 161 MspA1I CmG'CkG Cuts at: 0 146 451 1216 1468 1893 Size: 146 305 765 252 425 Fragments arranged by size: 765 425 305 252 146 NspI r_CATG'y Cuts at: 0 202 1737 1893 Size: 202 1535 156 Fragments arranged by size: 1535 202 156 PpuMI rG'GwC_Cy Cuts at: 0 48 1893 Size: 48 1845 PshAI GACnn'nnGTC Cuts at: 0 1216 1893 Size: 1216 677 PsiI TTA'TAA Cuts at: 0 229 1893 Size: 229 1664 PspOMI G'GGCC_C Cuts at: 0 103 643 1893 Size: 103 540 1250 Fragments arranged by size: 1250 540 103 PspXI vC'TCGA_Gb Cuts at: 0 1774 1893 Size: 1774 119 PstI C_TGCA'G Cuts at: 0 130 1467 1485 1893 Size: 130 1337 18 408 Fragments arranged by size: 1337 408 130 18 PvuII CAG'CTG Cuts at: 0 451 1216 1468 1893 Size: 451 765 252 425 Fragments arranged by size: 765 451 425 252 SacI G_AGCT'C Cuts at: 0 767 798 1893 Size: 767 31 1095 Fragments arranged by size: 1095 767 31 SapI GCTCTTCn'nnn_ Cuts at: 0 1109 1893 Size: 1109 784 ScaI AGT'ACT Cuts at: 0 1374 1893 Size: 1374 519 SexAI A'CCwGG_T Cuts at: 0 872 1893 Size: 872 1021 SfcI C'TryA_G Cuts at: 0 126 740 1463 1481 1814 1893 Size: 126 614 723 18 333 79 Fragments arranged by size: 723 614 333 126 79 18 SmlI C'TyrA_G Cuts at: 0 619 651 826 1774 1893 Size: 619 32 175 948 119 Fragments arranged by size: 948 619 175 119 32 SpeI A'CTAG_T Cuts at: 0 961 1893 Size: 961 932 SphI G_CATG'C Cuts at: 0 202 1893 Size: 202 1691 StyI C'CwwG_G Cuts at: 0 410 1893 Size: 410 1483 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 685 1893 Size: 685 1208 TatI w'GTAC_w Cuts at: 0 426 1372 1599 1821 1893 Size: 426 946 227 222 72 Fragments arranged by size: 946 426 227 222 72 Tth111I GACn'n_nGTC Cuts at: 0 1280 1893 Size: 1280 613 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1286 1893 Size: 1286 607 XhoI C'TCGA_G Cuts at: 0 1774 1893 Size: 1774 119 Enzymes that do cut: AcuI AflII AflIII AgeI AhdI AlfI AlwNI ApaI ApoI AseI AvaI BanI BanII BbsI BbvCI BcgI BclI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI BsaWI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI MluI MmeI MscI MslI MspA1I NspI PpuMI PshAI PsiI PspOMI PspXI PstI PvuII SacI SapI ScaI SexAI SfcI SmlI SpeI SphI StyI TaqII TatI Tth111I XcmI XhoI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AleI AloI ApaLI AscI AsiSI AvrII BaeI BamHI BbeI BciVI BfrBI BglI BglII BmtI BplI BpuEI BsaBI BsaHI BsaXI BsgI BsiEI BsiWI BspMI BsrBI BsrDI BssHII BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV FseI KasI KpnI MfeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PsrI PvuI RsrII SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI SspI StuI SwaI TstI XbaI XmaI XmnI ZraI