(Circular) (Six-base) MAPSORT of: pFN21AE4484.seq Check: 7034 from: 1 to: 6205 pFN21AE4484 6205 bp With 182 enzymes: SgfI * March 28, 2012 13:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4015 278 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3548 1937 Size: 1611 4594 Acc65I G'GTAC_C Cuts at: 3526 3526 Size: 6205 AclI AA'CG_TT Cuts at: 1563 4332 4705 1563 Size: 2769 373 3063 Fragments arranged by size: 3063 2769 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4268 5652 5920 1632 Size: 2636 1384 268 1917 Fragments arranged by size: 2636 1917 1384 268 AfeI AGC'GCT Cuts at: 3172 3172 Size: 6205 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6008 AflIII A'CryG_T Cuts at: 1203 3246 5104 6081 1203 Size: 2043 1858 977 1327 Fragments arranged by size: 2043 1858 1327 977 AgeI A'CCGG_T Cuts at: 5036 5036 Size: 6205 AhdI GACnn_n'nnGTC Cuts at: 4934 4934 Size: 6205 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6205 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6205 AlwNI CAG_nnn'CTG Cuts at: 2761 3948 5520 2761 Size: 1187 1572 3446 Fragments arranged by size: 3446 1572 1187 ApaI G_GGCC'C Cuts at: 2604 3483 2604 Size: 879 5326 ApaLI G'TGCA_C Cuts at: 4262 5418 4262 Size: 1156 5049 ApoI r'AATT_y Cuts at: 1493 2988 3514 3740 3892 6001 1493 Size: 1495 526 226 152 2109 1697 Fragments arranged by size: 2109 1697 1495 526 226 152 AseI AT'TA_AT Cuts at: 160 1032 2751 4759 160 Size: 872 1719 2008 1606 Fragments arranged by size: 2008 1719 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 AvaI C'yCGr_G Cuts at: 1870 1943 3530 1870 Size: 73 1587 4545 Fragments arranged by size: 4545 1587 73 BamHI G'GATC_C Cuts at: 3535 3902 3535 Size: 367 5838 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2488 3526 4981 618 Size: 325 217 627 701 1038 1455 1842 Fragments arranged by size: 1842 1455 1038 701 627 325 217 BanII G_rGCy'C Cuts at: 1391 2584 2604 3483 3524 1391 Size: 1193 20 879 41 4072 Fragments arranged by size: 4072 1193 879 41 20 BbeI G_GCGC'C Cuts at: 2492 2492 Size: 6205 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2122 2716 2731 928 Size: 971 223 594 15 4402 Fragments arranged by size: 4402 971 594 223 15 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4396 4430 1903 Size: 34 50 34 2375 34 3678 Fragments arranged by size: 3678 2375 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2027 2246 4097 5307 2027 Size: 219 1851 1210 2925 Fragments arranged by size: 2925 1851 1210 219 BclI T'GATC_A Cuts at: 1548 2817 1548 Size: 1269 4936 BfrBI ATG'CAT Cuts at: 2108 2108 Size: 6205 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4816 136 Size: 107 122 71 631 605 3144 1525 Fragments arranged by size: 3144 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2677 2698 6200 2677 Size: 21 3502 2682 Fragments arranged by size: 3502 2682 21 BlpI GC'TnA_GC Cuts at: 3113 3622 3113 Size: 509 5696 Bme1580I G_kGCm'C Cuts at: 1790 2604 3483 4266 5422 1790 Size: 814 879 783 1156 2573 Fragments arranged by size: 2573 1156 879 814 783 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6205 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3283 3936 4894 Size: 416 53 83 399 24 1866 653 958 Cuts at: 4894 442 Size: 1753 Fragments arranged by size: 1866 1753 958 653 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3044 1056 Size: 1988 4217 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2598 3151 3438 4865 1239 Size: 1359 553 287 1427 2579 Fragments arranged by size: 2579 1427 1359 553 287 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2666 4330 5195 5493 2666 Size: 1664 865 298 3378 Fragments arranged by size: 3378 1664 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3392 4868 882 Size: 617 1893 1476 2219 Fragments arranged by size: 2219 1893 1476 617 BsaAI yAC'GTr Cuts at: 493 1204 3996 493 Size: 711 2792 2702 Fragments arranged by size: 2792 2702 711 BsaBI GATnn'nnATC Cuts at: 6199 6199 Size: 6205 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2489 3285 4012 4394 Size: 53 83 186 936 956 796 727 382 Cuts at: 4394 5029 275 Size: 635 1451 Fragments arranged by size: 1451 956 936 796 727 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4638 5036 5310 5457 5868 4638 Size: 398 274 147 411 4975 Fragments arranged by size: 4975 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2013 2043 1178 Size: 30 165 30 610 30 5340 Fragments arranged by size: 5340 610 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3503 1186 Size: 2317 3888 BseYI C'CCAG_C Cuts at: 2055 2061 3416 5408 5904 2055 Size: 6 1355 1992 496 2356 Fragments arranged by size: 2356 1992 1355 496 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5632 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3655 4416 5444 1540 Size: 275 186 1654 761 1028 2301 Fragments arranged by size: 2301 1654 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4266 4351 5422 4266 Size: 85 1071 5049 Fragments arranged by size: 5049 1071 85 BsmI GAATG_Cn' Cuts at: 3203 3716 3809 3203 Size: 513 93 5599 Fragments arranged by size: 5599 513 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2584 2604 3483 3524 4266 4351 5422 Size: 399 794 20 879 41 742 85 1071 Cuts at: 5422 1391 Size: 2174 Fragments arranged by size: 2174 1071 879 794 742 399 85 41 20 BspEI T'CCGG_A Cuts at: 5868 5868 Size: 6205 BspHI T'CATG_A Cuts at: 2268 4094 5824 2268 Size: 1826 1730 2649 Fragments arranged by size: 2649 1826 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3560 844 Size: 2716 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4092 1394 Size: 429 2269 3507 Fragments arranged by size: 3507 2269 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4700 4874 65 Size: 1183 3452 174 1396 Fragments arranged by size: 3452 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4849 5036 1700 Size: 110 3039 187 2869 Fragments arranged by size: 3039 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6205 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6205 BssSI C'ACGA_G Cuts at: 4265 5277 4265 Size: 1012 5193 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6205 BstBI TT'CG_AA Cuts at: 5009 5009 Size: 6205 BstEII G'GTnAC_C Cuts at: 1184 3184 1184 Size: 2000 4205 BstXI CCAn_nnnn'nTGG Cuts at: 2200 2449 3175 2200 Size: 249 726 5230 Fragments arranged by size: 5230 726 249 BstYI r'GATC_y Cuts at: 1972 2677 2698 3535 3902 4287 4304 5756 5842 Size: 705 21 837 367 385 17 1452 86 Cuts at: 5842 5854 6200 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 837 705 385 367 346 86 21 17 12 BtgI C'CryG_G Cuts at: 513 1065 2454 513 Size: 552 1389 4264 Fragments arranged by size: 4264 1389 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5466 Fragments arranged by size: 5466 683 56 BtsI GCAGTG_nn' Cuts at: 2073 3721 4515 4535 2073 Size: 1648 794 20 3743 Fragments arranged by size: 3743 1648 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6205 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6170 DraI TTT'AAA Cuts at: 3509 3509 Size: 6205 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5212 809 Size: 507 3896 1802 Fragments arranged by size: 3896 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2439 2451 3652 Size: 54 1425 50 275 18 609 12 1201 Cuts at: 3652 4541 6170 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1201 889 609 275 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 3652 1537 Size: 275 1840 4090 Fragments arranged by size: 4090 1840 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2864 3320 3390 4135 1353 Size: 1511 456 70 745 3423 Fragments arranged by size: 3423 1511 745 456 70 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4792 5166 5312 387 Size: 1064 138 3203 374 146 1280 Fragments arranged by size: 3203 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2598 3151 3438 4268 4865 5652 5920 Size: 393 966 553 287 830 597 787 268 Cuts at: 5920 1239 Size: 1524 Fragments arranged by size: 1524 966 830 787 597 553 393 287 268 EcoNI CCTnn'n_nnAGG Cuts at: 2498 2498 Size: 6205 EcoO109I rG'GnC_Cy Cuts at: 2222 3479 2222 Size: 1257 4948 EcoRI G'AATT_C Cuts at: 2988 3514 3892 2988 Size: 526 378 5301 Fragments arranged by size: 5301 526 378 EcoRV GAT'ATC Cuts at: 2187 2187 Size: 6205 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2660 2917 2660 Size: 257 5948 FspI TGC'GCA Cuts at: 4711 4711 Size: 6205 HaeII r_GCGC'y Cuts at: 1678 2492 3174 5352 1678 Size: 814 682 2178 2531 Fragments arranged by size: 2531 2178 814 682 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2211 2243 2717 2749 3060 Size: 32 135 32 639 32 474 32 311 Cuts at: 3060 3092 4846 4878 4920 4952 1373 Size: 32 1754 32 42 32 2626 Fragments arranged by size: 2626 1754 639 474 311 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2929 3549 3795 669 Size: 988 281 991 620 246 3079 Fragments arranged by size: 3079 991 988 620 281 246 HindIII A'AGCT_T Cuts at: 748 2533 748 Size: 1785 4420 HpaI GTT'AAC Cuts at: 3795 3795 Size: 6205 KasI G'GCGC_C Cuts at: 2488 2488 Size: 6205 KpnI G_GTAC'C Cuts at: 3530 3530 Size: 6205 MfeI C'AATT_G Cuts at: 3804 3804 Size: 6205 MluI A'CGCG_T Cuts at: 6081 6081 Size: 6205 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1999 2011 3046 3130 5319 5503 1305 Size: 694 12 1035 84 2189 184 2007 Fragments arranged by size: 2189 2007 1035 694 184 84 12 MscI TGG'CCA Cuts at: 10 64 1489 1832 2441 2453 10 Size: 54 1425 343 609 12 3762 Fragments arranged by size: 3762 1425 609 343 54 12 MslI CAynn'nnrTG Cuts at: 518 1166 3323 4163 4522 4681 518 Size: 648 2157 840 359 159 2042 Fragments arranged by size: 2157 2042 840 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3621 4298 5446 5691 1606 Size: 2015 677 1148 245 2120 Fragments arranged by size: 2120 2015 1148 677 245 NarI GG'CG_CC Cuts at: 2489 2489 Size: 6205 NcoI C'CATG_G Cuts at: 513 1065 2454 513 Size: 552 1389 4264 Fragments arranged by size: 4264 1389 552 NdeI CA'TA_TG Cuts at: 387 3036 387 Size: 2649 3556 NheI G'CTAG_C Cuts at: 1052 3040 1052 Size: 1988 4217 NotI GC'GGCC_GC Cuts at: 3652 3652 Size: 6205 NsiI A_TGCA'T Cuts at: 2110 2110 Size: 6205 NspI r_CATG'y Cuts at: 1132 1229 3250 3282 3563 5108 1132 Size: 97 2021 32 281 1545 2229 Fragments arranged by size: 2229 2021 1545 281 97 32 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6205 PciI A'CATG_T Cuts at: 3246 5104 3246 Size: 1858 4347 PflMI CCAn_nnn'nTGG Cuts at: 1108 2018 1108 Size: 910 5295 PfoI T'CCnGG_A Cuts at: 2760 2760 Size: 6205 PmeI GTTT'AAAC Cuts at: 3509 3509 Size: 6205 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4274 4306 5787 5819 4274 Size: 32 1481 32 4660 Fragments arranged by size: 4660 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2222 2222 Size: 6205 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6205 PsiI TTA'TAA Cuts at: 3775 3775 Size: 6205 PspOMI G'GGCC_C Cuts at: 2600 3479 2600 Size: 879 5326 PstI C_TGCA'G Cuts at: 830 3557 830 Size: 2727 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6205 SalI G'TCGA_C Cuts at: 1936 3547 1936 Size: 1611 4594 SbfI CC_TGCA'GG Cuts at: 3557 3557 Size: 6205 ScaI AGT'ACT Cuts at: 4453 6165 4453 Size: 1712 4493 SfcI C'TryA_G Cuts at: 826 1045 3553 4688 5369 5560 826 Size: 219 2508 1135 681 191 1471 Fragments arranged by size: 2508 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2490 2490 Size: 6205 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6205 SmaI CCC'GGG Cuts at: 1872 3532 1872 Size: 1660 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2645 4309 5210 5472 820 Size: 197 926 702 1664 901 262 1553 Fragments arranged by size: 1664 1553 926 901 702 262 197 SnaBI TAC'GTA Cuts at: 493 3996 493 Size: 3503 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6205 SphI G_CATG'C Cuts at: 1132 3563 1132 Size: 2431 3774 SspI AAT'ATT Cuts at: 5 52 4129 5 Size: 47 4077 2081 Fragments arranged by size: 4077 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6205 StyI C'CwwG_G Cuts at: 513 1065 2454 2613 3483 3644 513 Size: 552 1389 159 870 161 3074 Fragments arranged by size: 3074 1389 870 552 161 159 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2795 4232 4249 4402 4587 5032 325 Size: 1255 1215 1437 17 153 185 445 1498 Fragments arranged by size: 1498 1437 1255 1215 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2480 4451 6163 Size: 274 80 33 51 1443 503 1971 1712 Cuts at: 6163 96 Size: 138 Fragments arranged by size: 1971 1712 1443 503 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6165 6197 6165 Size: 32 6173 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6205 XbaI T'CTAG_A Cuts at: 3541 3541 Size: 6205 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2052 2052 Size: 6205 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6205 XmaI C'CCGG_G Cuts at: 1870 3530 1870 Size: 1660 4545 XmnI GAAnn'nnTTC Cuts at: 4334 4334 Size: 6205 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4013 276 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI