(Linear) (Six-base) MAPSORT of: KIEE4484.seq Check: 4341 from: 1 to: 1500 KIEE4484.orf With 182 enzymes: SgfI * March 22, 2012 18:10 .. AfeI AGC'GCT Cuts at: 0 1167 1500 Size: 1167 333 AflIII A'CryG_T Cuts at: 0 1241 1500 Size: 1241 259 AlwNI CAG_nnn'CTG Cuts at: 0 756 1500 Size: 756 744 ApaI G_GGCC'C Cuts at: 0 599 1478 1500 Size: 599 879 22 Fragments arranged by size: 879 599 22 ApoI r'AATT_y Cuts at: 0 983 1500 Size: 983 517 AseI AT'TA_AT Cuts at: 0 746 1500 Size: 746 754 BanI G'GyrC_C Cuts at: 0 483 1500 Size: 483 1017 BanII G_rGCy'C Cuts at: 0 579 599 1478 1500 Size: 579 20 879 22 Fragments arranged by size: 879 579 22 20 BbeI G_GCGC'C Cuts at: 0 487 1500 Size: 487 1013 BbsI GAAGACnn'nnnn_ Cuts at: 0 117 711 726 1500 Size: 117 594 15 774 Fragments arranged by size: 774 594 117 15 BciVI GTATCCnnnnn_n' Cuts at: 0 22 241 1500 Size: 22 219 1259 Fragments arranged by size: 1259 219 22 BclI T'GATC_A Cuts at: 0 812 1500 Size: 812 688 BfrBI ATG'CAT Cuts at: 0 103 1500 Size: 103 1397 BglII A'GATC_T Cuts at: 0 672 693 1500 Size: 672 21 807 Fragments arranged by size: 807 672 21 BlpI GC'TnA_GC Cuts at: 0 1108 1500 Size: 1108 392 Bme1580I G_kGCm'C Cuts at: 0 599 1478 1500 Size: 599 879 22 Fragments arranged by size: 879 599 22 BmrI ACTGGGnnnn_n' Cuts at: 0 1278 1500 Size: 1278 222 BmtI G_CTAG'C Cuts at: 0 1039 1500 Size: 1039 461 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 593 1146 1433 1500 Size: 593 553 287 67 Fragments arranged by size: 593 553 287 67 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 661 1500 Size: 661 839 BsaI GGTCTCn'nnnn_ Cuts at: 0 1387 1500 Size: 1387 113 BsaHI Gr'CG_yC Cuts at: 0 484 1280 1500 Size: 484 796 220 Fragments arranged by size: 796 484 220 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 8 38 1500 Size: 8 30 1462 Fragments arranged by size: 1462 30 8 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1498 1500 Size: 1498 2 BseYI C'CCAG_C Cuts at: 0 50 56 1411 1500 Size: 50 6 1355 89 Fragments arranged by size: 1355 89 50 6 BsmI GAATG_Cn' Cuts at: 0 1198 1500 Size: 1198 302 Bsp1286I G_dGCh'C Cuts at: 0 579 599 1478 1500 Size: 579 20 879 22 Fragments arranged by size: 879 579 22 20 BspHI T'CATG_A Cuts at: 0 263 1500 Size: 263 1237 BstEII G'GTnAC_C Cuts at: 0 1179 1500 Size: 1179 321 BstXI CCAn_nnnn'nTGG Cuts at: 0 195 444 1170 1500 Size: 195 249 726 330 Fragments arranged by size: 726 330 249 195 BstYI r'GATC_y Cuts at: 0 672 693 1500 Size: 672 21 807 Fragments arranged by size: 807 672 21 BtgI C'CryG_G Cuts at: 0 449 1500 Size: 449 1051 BtsI GCAGTG_nn' Cuts at: 0 68 1500 Size: 68 1432 EaeI y'GGCC_r Cuts at: 0 434 446 1500 Size: 434 12 1054 Fragments arranged by size: 1054 434 12 EarI CTCTTCn'nnn_ Cuts at: 0 859 1315 1385 1500 Size: 859 456 70 115 Fragments arranged by size: 859 456 115 70 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 593 1146 1433 1500 Size: 593 553 287 67 Fragments arranged by size: 593 553 287 67 EcoNI CCTnn'n_nnAGG Cuts at: 0 493 1500 Size: 493 1007 EcoO109I rG'GnC_Cy Cuts at: 0 217 1474 1500 Size: 217 1257 26 Fragments arranged by size: 1257 217 26 EcoRI G'AATT_C Cuts at: 0 983 1500 Size: 983 517 EcoRV GAT'ATC Cuts at: 0 182 1500 Size: 182 1318 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 655 912 1500 Size: 655 257 588 Fragments arranged by size: 655 588 257 HaeII r_GCGC'y Cuts at: 0 487 1169 1500 Size: 487 682 331 Fragments arranged by size: 682 487 331 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 206 238 712 744 1055 1087 1500 Size: 206 32 474 32 311 32 413 Fragments arranged by size: 474 413 311 206 32 32 32 HincII GTy'rAC Cuts at: 0 924 1500 Size: 924 576 HindIII A'AGCT_T Cuts at: 0 528 1500 Size: 528 972 KasI G'GCGC_C Cuts at: 0 483 1500 Size: 483 1017 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 6 1041 1125 1500 Size: 6 1035 84 375 Fragments arranged by size: 1035 375 84 6 MscI TGG'CCA Cuts at: 0 436 448 1500 Size: 436 12 1052 Fragments arranged by size: 1052 436 12 MslI CAynn'nnrTG Cuts at: 0 1318 1500 Size: 1318 182 NarI GG'CG_CC Cuts at: 0 484 1500 Size: 484 1016 NcoI C'CATG_G Cuts at: 0 449 1500 Size: 449 1051 NdeI CA'TA_TG Cuts at: 0 1031 1500 Size: 1031 469 NheI G'CTAG_C Cuts at: 0 1035 1500 Size: 1035 465 NsiI A_TGCA'T Cuts at: 0 105 1500 Size: 105 1395 NspI r_CATG'y Cuts at: 0 1245 1277 1500 Size: 1245 32 223 Fragments arranged by size: 1245 223 32 PciI A'CATG_T Cuts at: 0 1241 1500 Size: 1241 259 PflMI CCAn_nnn'nTGG Cuts at: 0 13 1500 Size: 13 1487 PfoI T'CCnGG_A Cuts at: 0 755 1500 Size: 755 745 PpuMI rG'GwC_Cy Cuts at: 0 217 1500 Size: 217 1283 PspOMI G'GGCC_C Cuts at: 0 595 1474 1500 Size: 595 879 26 Fragments arranged by size: 879 595 26 SfoI GGC'GCC Cuts at: 0 485 1500 Size: 485 1015 SmlI C'TyrA_G Cuts at: 0 640 1500 Size: 640 860 StyI C'CwwG_G Cuts at: 0 449 608 1478 1500 Size: 449 159 870 22 Fragments arranged by size: 870 449 159 22 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 790 1500 Size: 790 710 TatI w'GTAC_w Cuts at: 0 475 1500 Size: 475 1025 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 47 1500 Size: 47 1453 Enzymes that do cut: AfeI AflIII AlwNI ApaI ApoI AseI BanI BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BmtI BpmI BpuEI BsaI BsaHI BsaXI BseRI BseYI BsmI Bsp1286I BspHI BstEII BstXI BstYI BtgI BtsI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII KasI MmeI MscI MslI NarI NcoI NdeI NheI NsiI NspI PciI PflMI PfoI PpuMI PspOMI SfoI SmlI StyI TaqII TatI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AflII AgeI AhdI AleI AlfI AloI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BglI BmgBI BplI Bpu10I BsaAI BsaBI BsaWI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI FseI FspI FspAI HpaI KpnI MfeI MluI MspA1I NaeI NgoMIV NotI NruI PacI PasI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI XmaI XmnI ZraI