################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaHI BsaI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MseI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccIII 423 TCCGGA T^CCGGA ApaI 4 GGGCCC GGGCC^C AspI 679 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BclI 944 TGATCA T^GATCA BcnI 555 CCCGG CC^[GC]GG BfaI 995 CTAG C^TAG BglI 1030 GCCAGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 100 GCTCAGC GC^T[ACGT]AGC BpmI 447 CTCCAG CTGGAG(16/14) Bpu1102I 100 GCTCAGC GC^T[ACGT]AGC BsaWI 423 TCCGGA [AT]^CCGG[AT] BseAI 423 TCCGGA T^CCGGA BsmFI 563 GTCCC GGGAC(10/14) BspEI 423 TCCGGA T^CCGGA BspHI 1013 TCATGA T^CATGA BssSI 185 CTCGTG CACGAG(-5/-1) BstXI 461 CCAACCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 100 GCTCAGC GC^T[ACGT]AGC CfoI 33 GCGC GCG^C DsaI 467 CCGTGG C^C[GA][CT]GG EcoT22I 311 ATGCAT ATGCA^T FauI 87 CCCGC CCCGC(4/6) FbaI 944 TGATCA T^GATCA HhaI 33 GCGC GCG^C HinP1I 33 GCGC G^CGC MaeI 995 CTAG C^TAG MroI 423 TCCGGA T^CCGGA NciI 555 CCCGG CC^[GC]GG NsiI 311 ATGCAT ATGCA^T PflFI 679 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 930 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 574 CAGCTG CAG^CTG RcaI 1013 TCATGA T^CATGA ScaI 1023 AGTACT AGT^ACT Tsp509I 234 AATT ^AATT Tth111I 679 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 177 CCAGGACCCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### BalI 405 TGGCCA TGG^CCA 1028 TGGCCA BamHI 352 GGATCC G^GATCC 883 GGATCC BanII 4 GGGCCC G[GA]GC[CT]^C 49 GGGCTC BmyI 4 GGGCCC G[AGT]GC[ACT]^C 49 GGGCTC Bsp1286I 4 GGGCCC G[AGT]GC[ACT]^C 49 GGGCTC BstYI 352 GGATCC [GA]^GATC[CT] 883 GGATCC Cac8I 276 GCCTGC GC[ACGT]^[ACGT]GC 892 GCCTGC HapII 424 CCGG C^CGG 556 CCGG HpaII 424 CCGG C^CGG 556 CCGG MaeII 332 ACGT A^CGT 626 ACGT MboII 394 GAAGA GAAGA(8/7) 153 TCTTC MflI 352 GGATCC [GA]^GATC[CT] 883 GGATCC MluNI 405 TGGCCA TGG^CCA 1028 TGGCCA MscI 405 TGGCCA TGG^CCA 1028 TGGCCA MspA1I 574 CAGCTG C[AC]G^C[GT]G 879 CAGCGG MspI 424 CCGG C^CGG 556 CCGG TaqI 231 TCGA T^CGA 291 TCGA TthHB8I 231 TCGA T^CGA 291 TCGA XhoII 352 GGATCC [GA]^GATC[CT] 883 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaII BbvI BsaJI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboI MnlI MvaI MwoI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TseI Tsp45I TspRI