################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MroI MseI MunI MvnI NarI NciI NcoI NdeI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AccI 636 GTCTAC GT^[AC][GT]AC AfaI 500 GTAC GT^AC Asp700I 789 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 2 TGGCCA TGG^CCA BpmI 560 CTCCAG CTGGAG(16/14) BsgI 77 GTGCAG GTGCAG(16/14) BspMI 534 ACCTGC ACCTGC(4/8) BsrFI 205 GCCGGC [GA]^CCGG[CT] BsrI 355 CCAGT ACTGG(1/-1) BstYI 441 AGATCC [GA]^GATC[CT] Cfr10I 205 GCCGGC [GA]^CCGG[CT] EcoT14I 63 CCAAGG C^C[AT][AT]GG HapII 206 CCGG C^CGG HpaII 206 CCGG C^CGG MflI 441 AGATCC [GA]^GATC[CT] MluNI 2 TGGCCA TGG^CCA MscI 2 TGGCCA TGG^CCA MspI 206 CCGG C^CGG NaeI 205 GCCGGC GCC^GGC NgoMIV 205 GCCGGC G^CCGGC NheI 238 GCTAGC G^CTAGC PstI 749 CTGCAG CTGCA^G RsaI 500 GTAC GT^AC SapI 82 GAAGAGC GCTCTTC(1/4) StyI 63 CCAAGG C^C[AT][AT]GG TaqI 833 TCGA T^CGA TthHB8I 833 TCGA T^CGA XcmI 47 CCACGAGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 441 AGATCC [GA]^GATC[CT] XmnI 789 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 156 GAATTT [GA]^AATT[CT] 481 GAATTT AlwI 442 GATCC GGATC(4/5) 119 GGATC AlwNI 148 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 641 CAGAAACTG ApoI 156 GAATTT [GA]^AATT[CT] 481 GAATTT BsmFI 192 GGGAC GGGAC(10/14) 706 GTCCC BsmI 416 GCATTC GAATGC(1/-1) 673 GCATTC BssSI 48 CACGAG CACGAG(-5/-1) 411 CTCGTG BstF5I 93 CATCC GGATG(2/0) 515 CATCC DdeI 170 CTAAG C^T[ACGT]AG 196 CTAAG DsaI 580 CCACGG C^C[GA][CT]GG 710 CCACGG EaeI 2 TGGCCA [CT]^GGCC[GA] 203 TGGCCG FokI 93 CATCC GGATG(9/13) 515 CATCC HphI 122 TCACC GGTGA(8/7) 431 TCACC MslI 93 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 123 CACCCTTGTG MspA1I 151 CAGCTG C[AC]G^C[GT]G 752 CAGCTG NlaIII 104 CATG CATG^ 633 CATG NlaIV 53 GGCTCC GG[ACGT]^[ACGT]CC 351 GGTTCC PleI 916 GAGTC GAGTC(4/5) 924 GAGTC PvuII 151 CAGCTG CAG^CTG 752 CAGCTG SfaNI 947 GATGC GCATC(5/9) 514 GCATC StuI 176 AGGCCT AGG^CCT 791 AGGCCT Tsp45I 494 GTGAC ^GT[GC]AC 604 GTCAC TspRI 356 CAGTG CA[GC]TG[ACGT][ACGT]^ 492 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AluI AvaII BbvI BfaI BsaJI BseRI BsiYI BslI BssKI BstNI Cac8I Cfr13I DpnII EarI EcoRII Fnu4HI HaeIII HinfI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MvaI MwoI NdeII Sau3AI Sau96I ScrFI SfcI TfiI TseI Tsp509I