Restriction map for
pFN21AE4509
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BbrPI BciVI
BfrI BglI BglII BlnI BsaBI
BseAI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI Eam1105I
Eco81I EcoRI EcoRV EcoT22I FseI
HincII HindII HindIII HpaI KpnI
MamI MfeI MluI MroI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SpeI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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ApaI 61 GGGCCC GGGCC^C
BamHI 424 GGATCC G^GATCC
BbsI 733 GAAGAC GAAGAC(2/6)
BbvCI 1468 GCTGAGG CCTCAGC(-5/-2)
BclI 263 TGATCA T^GATCA
BpuAI 733 GAAGAC GAAGAC(2/6)
BsaI 16 GAGACC GGTCTC(1/5)
BsgI 1409 GTGCAG GTGCAG(16/14)
BsiEI 56 CGGCCG CG[GA][CT]^CG
BsiWI 565 CGTACG C^GTACG
BsmBI 496 GAGACG CGTCTC(1/5)
BsmI 1415 GCATTC GAATGC(1/-1)
BspHI 1326 TCATGA T^CATGA
DraIII 374 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 438 GACCCTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 56 CGGCCG C^GGCCG
EclXI 56 CGGCCG C^GGCCG
Eco52I 56 CGGCCG C^GGCCG
EcoNI 759 CCTCAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 263 TGATCA T^GATCA
MseI 709 TTAA T^TAA
NspI 251 GCATGC [GA]CATG^[CT]
PpuMI 1280 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 285 CTGCAG CTGCA^G
RcaI 1326 TCATGA T^CATGA
SapI 6 GAAGAGC GCTCTTC(1/4)
SfcI 285 CTGCAG C^T[GA][CT]AG
SphI 251 GCATGC GCATG^C
Tru9I 709 TTAA T^TAA
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# TWO CUT ENZYMES ( 43 enzymes) #
###################################
Aor51HI 37 AGCGCT AGC^GCT
126 AGCGCT
AspHI 68 GAGCTC G[AT]GC[AT]^C
1198 GAGCTC
AvaI 328 CTCGGG C^[CT]CG[GA]G
471 CTCGGG
BalI 521 TGGCCA TGG^CCA
1556 TGGCCA
BanI 119 GGCGCC G^G[CT][GA]CC
301 GGCGCC
BbeI 119 GGCGCC GGCGC^C
301 GGCGCC
BcgI 770 CGACGATGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
344 GCATCGCTGTCG
BfaI 718 CTAG C^TAG
1228 CTAG
BlpI 192 GCTCAGC GC^T[ACGT]AGC
1034 GCTCAGC
Bpu1102I 192 GCTCAGC GC^T[ACGT]AGC
1034 GCTCAGC
BsaAI 567 TACGTA [CT]AC^GT[GA]
802 TACGTA
BsaWI 427 TCCGGT [AT]^CCGG[AT]
1367 ACCGGA
BsiHKAI 68 GAGCTC G[AT]GC[AT]^C
1198 GAGCTC
BsoBI 328 CTCGGG C^[CT]CG[GA]G
471 CTCGGG
BsrDI 1176 CATTGC GCAATG(2/0)
1290 CATTGC
BstEII 213 GGTCACC G^GT[ACGT]ACC
870 GGTGACC
BstPI 213 GGTCACC G^GT[ACGT]ACC
870 GGTGACC
BstYI 424 GGATCC [GA]^GATC[CT]
632 AGATCC
CelII 192 GCTCAGC GC^T[ACGT]AGC
1034 GCTCAGC
EarI 6 GAAGAG CTCTTC(1/4)
1069 GAAGAG
Eco47III 37 AGCGCT AGC^GCT
126 AGCGCT
EcoO65I 213 GGTCACC G^GT[ACGT]ACC
870 GGTGACC
EcoT14I 401 CCTTGG C^C[AT][AT]GG
1580 CCAAGG
KasI 119 GGCGCC G^GCGCC
301 GGCGCC
Ksp632I 6 GAAGAG CTCTTC(1/4)
1069 GAAGAG
KspI 80 CCGCGG CCGC^GG
1166 CCGCGG
MaeI 718 CTAG C^TAG
1228 CTAG
MflI 424 GGATCC [GA]^GATC[CT]
632 AGATCC
MluNI 521 TGGCCA TGG^CCA
1556 TGGCCA
MscI 521 TGGCCA TGG^CCA
1556 TGGCCA
NarI 119 GGCGCC GG^CGCC
301 GGCGCC
PflMI 123 CCAAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1155 CCAGTGTGTGG
PvuII 321 CAGCTG CAG^CTG
1271 CAGCTG
SacI 68 GAGCTC GAGCT^C
1198 GAGCTC
SacII 80 CCGCGG CCGC^GG
1166 CCGCGG
SfoI 119 GGCGCC GGC^GCC
301 GGCGCC
SnaBI 567 TACGTA TAC^GTA
802 TACGTA
StyI 401 CCTTGG C^C[AT][AT]GG
1580 CCAAGG
TaqI 314 TCGA T^CGA
515 TCGA
Tsp509I 754 AATT ^AATT
930 AATT
TthHB8I 314 TCGA T^CGA
515 TCGA
Van91I 123 CCAAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1155 CCAGTGTGTGG
XhoII 424 GGATCC [GA]^GATC[CT]
632 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaII AviII BanII BbvI
BcnI BmyI BpmI BsaHI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI TfiI
TseI Tsp45I TspRI XcmI