(Circular) (Six-base) MAPSORT of: pFN21AE4509.seq Check: 3942 from: 1 to: 6301 pFN21AE4509 6301 bp With 182 enzymes: SgfI * March 28, 2012 14:06 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4111 278 Size: 53 83 186 936 2575 2468 Fragments arranged by size: 2575 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3644 1937 Size: 1707 4594 Acc65I G'GTAC_C Cuts at: 3622 3622 Size: 6301 AclI AA'CG_TT Cuts at: 1563 4428 4801 1563 Size: 2865 373 3063 Fragments arranged by size: 3063 2865 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2432 4364 5748 6016 1632 Size: 800 1932 1384 268 1917 Fragments arranged by size: 1932 1917 1384 800 268 AfeI AGC'GCT Cuts at: 2044 2133 2044 Size: 89 6212 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6104 AflIII A'CryG_T Cuts at: 1203 5200 6177 1203 Size: 3997 977 1327 Fragments arranged by size: 3997 1327 977 AgeI A'CCGG_T Cuts at: 5132 5132 Size: 6301 AhdI GACnn_n'nnGTC Cuts at: 5030 5030 Size: 6301 AleI CACnn'nnGTG Cuts at: 1166 3383 3386 1166 Size: 2217 3 4081 Fragments arranged by size: 4081 2217 3 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2122 3486 1184 Size: 938 1364 3999 Fragments arranged by size: 3999 1364 938 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2922 2954 2922 Size: 32 6269 AlwNI CAG_nnn'CTG Cuts at: 4044 5616 4044 Size: 1572 4729 ApaI G_GGCC'C Cuts at: 2070 2070 Size: 6301 ApaLI G'TGCA_C Cuts at: 4358 5514 4358 Size: 1156 5145 ApoI r'AATT_y Cuts at: 1493 3610 3836 3988 6097 1493 Size: 2117 226 152 2109 1697 Fragments arranged by size: 2117 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4855 160 Size: 872 3823 1606 Fragments arranged by size: 3823 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 AvaI C'yCGr_G Cuts at: 1870 1943 2333 2476 3626 1870 Size: 73 390 143 1150 4545 Fragments arranged by size: 4545 1150 390 143 73 BamHI G'GATC_C Cuts at: 2429 3631 3998 2429 Size: 1202 367 4732 Fragments arranged by size: 4732 1202 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2124 2306 3622 5077 618 Size: 325 217 627 337 182 1316 1455 1842 Fragments arranged by size: 1842 1455 1316 627 337 325 217 182 BanII G_rGCy'C Cuts at: 1391 2070 2077 2199 3207 3620 1391 Size: 679 7 122 1008 413 4072 Fragments arranged by size: 4072 1008 679 413 122 7 BbeI G_GCGC'C Cuts at: 2128 2310 2128 Size: 182 6119 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2745 928 Size: 971 846 4484 Fragments arranged by size: 4484 971 846 BbvCI CC'TCA_GC Cuts at: 3474 3474 Size: 6301 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2338 2372 2764 2798 4492 4526 1903 Size: 34 401 34 392 34 1694 34 3678 Fragments arranged by size: 3678 1694 401 392 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4193 5403 4193 Size: 1210 5091 BclI T'GATC_A Cuts at: 1548 2268 1548 Size: 720 5581 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4912 136 Size: 107 122 71 631 605 3240 1525 Fragments arranged by size: 3240 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6296 6296 Size: 6301 BlpI GC'TnA_GC Cuts at: 2198 3040 3718 2198 Size: 842 678 4781 Fragments arranged by size: 4781 842 678 Bme1580I G_kGCm'C Cuts at: 1790 2070 3571 4362 5518 1790 Size: 280 1501 791 1156 2573 Fragments arranged by size: 2573 1501 1156 791 280 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6301 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4032 4990 442 Size: 416 53 83 399 24 2615 958 1753 Fragments arranged by size: 2615 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6301 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2759 3305 2759 Size: 546 5755 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2489 3019 3300 3329 4961 1239 Size: 1250 530 281 29 1632 2579 Fragments arranged by size: 2579 1632 1250 530 281 29 Bpu10I CC'TnA_GC Cuts at: 2046 2204 3474 2046 Size: 158 1270 4873 Fragments arranged by size: 4873 1270 158 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4426 5291 5589 4426 Size: 865 298 5138 Fragments arranged by size: 5138 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2015 4964 882 Size: 617 516 2949 2219 Fragments arranged by size: 2949 2219 617 516 BsaAI yAC'GTr Cuts at: 493 1204 2574 2809 4092 493 Size: 711 1370 235 1283 2702 Fragments arranged by size: 2702 1370 1283 711 235 BsaBI GATnn'nnATC Cuts at: 6295 6295 Size: 6301 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2125 2307 2338 4108 Size: 53 83 186 936 592 182 31 1770 Cuts at: 4108 4490 5125 275 Size: 382 635 1451 Fragments arranged by size: 1770 1451 936 635 592 382 186 182 83 53 31 BsaWI w'CCGG_w Cuts at: 2432 3372 4734 5132 5406 5553 5964 2432 Size: 940 1362 398 274 147 411 2769 Fragments arranged by size: 2769 1362 940 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2922 2952 1178 Size: 30 165 30 1519 30 4527 Fragments arranged by size: 4527 1519 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2475 2990 3527 3530 1186 Size: 1289 515 537 3 3957 Fragments arranged by size: 3957 1289 537 515 3 BseYI C'CCAG_C Cuts at: 5504 6000 5504 Size: 496 5805 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3435 1163 Size: 573 1699 4029 Fragments arranged by size: 4029 1699 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2064 3751 4512 5540 1540 Size: 275 186 63 1687 761 1028 2301 Fragments arranged by size: 2301 1687 1028 761 275 186 63 BsiHKAI G_wGCw'C Cuts at: 2077 3207 4362 4447 5518 2077 Size: 1130 1155 85 1071 2860 Fragments arranged by size: 2860 1155 1130 1071 85 BsiWI C'GTAC_G Cuts at: 2570 2570 Size: 6301 BsmI GAATG_Cn' Cuts at: 3420 3812 3905 3420 Size: 392 93 5816 Fragments arranged by size: 5816 392 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2495 2495 Size: 6301 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2077 2199 3207 3571 3620 4362 Size: 399 280 7 122 1008 364 49 742 Cuts at: 4362 4447 5518 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 1008 742 399 364 280 122 85 49 7 BspEI T'CCGG_A Cuts at: 5964 5964 Size: 6301 BspHI T'CATG_A Cuts at: 3331 4190 5920 3331 Size: 859 1730 3712 Fragments arranged by size: 3712 1730 859 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2206 3038 3096 3345 3656 844 Size: 1362 832 58 249 311 3489 Fragments arranged by size: 3489 1362 832 311 249 58 BsrBI CCG'CTC Cuts at: 1394 1823 4188 1394 Size: 429 2365 3507 Fragments arranged by size: 3507 2365 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3180 3294 4796 4970 65 Size: 1183 1932 114 1502 174 1396 Fragments arranged by size: 1932 1502 1396 1183 174 114 BsrFI r'CCGG_y Cuts at: 1700 1810 4945 5132 1700 Size: 110 3135 187 2869 Fragments arranged by size: 3135 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6301 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6301 BssSI C'ACGA_G Cuts at: 4361 5373 4361 Size: 1012 5289 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6301 BstBI TT'CG_AA Cuts at: 5105 5105 Size: 6301 BstEII G'GTnAC_C Cuts at: 1184 2218 2875 1184 Size: 1034 657 4610 Fragments arranged by size: 4610 1034 657 BstXI CCAn_nnnn'nTGG Cuts at: 2461 2554 3307 3385 2461 Size: 93 753 78 5377 Fragments arranged by size: 5377 753 93 78 BstYI r'GATC_y Cuts at: 1972 2429 2637 3631 3998 4383 4400 5852 5938 Size: 457 208 994 367 385 17 1452 86 Cuts at: 5938 5950 6296 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 994 457 385 367 346 208 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2085 2400 3171 3381 3558 513 Size: 552 939 81 315 771 210 177 3256 Fragments arranged by size: 3256 939 771 552 315 210 177 81 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2335 3507 487 Size: 683 56 1109 1172 3281 Fragments arranged by size: 3281 1172 1109 683 56 BtsI GCAGTG_nn' Cuts at: 3817 4611 4631 3817 Size: 794 20 5487 Fragments arranged by size: 5487 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6301 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6266 DraI TTT'AAA Cuts at: 3605 3605 Size: 6301 DraIII CAC_nnn'GTG Cuts at: 2384 2384 Size: 6301 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2449 5308 809 Size: 507 1133 2859 1802 Fragments arranged by size: 2859 1802 1133 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2061 2526 3168 Size: 54 1425 50 275 18 231 465 642 Cuts at: 3168 3561 3748 4637 6266 8 Size: 393 187 889 1629 43 Fragments arranged by size: 1629 1425 889 642 465 393 275 231 187 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2061 3748 1537 Size: 275 249 1687 4090 Fragments arranged by size: 4090 1687 275 249 EarI CTCTTCn'nnn_ Cuts at: 1353 2006 3069 4231 1353 Size: 653 1063 1162 3423 Fragments arranged by size: 3423 1162 1063 653 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4888 5262 5408 387 Size: 1064 138 3299 374 146 1280 Fragments arranged by size: 3299 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2075 3205 2075 Size: 1130 5171 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2432 2489 3019 3300 3329 4364 4961 Size: 393 800 57 530 281 29 1035 597 Cuts at: 4961 5748 6016 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1035 800 787 597 530 393 281 268 57 29 EcoNI CCTnn'n_nnAGG Cuts at: 2768 2768 Size: 6301 EcoO109I rG'GnC_Cy Cuts at: 2054 2480 3286 2054 Size: 426 806 5069 Fragments arranged by size: 5069 806 426 EcoRI G'AATT_C Cuts at: 3610 3988 3610 Size: 378 5923 FspI TGC'GCA Cuts at: 2264 2439 3186 4807 2264 Size: 175 747 1621 3758 Fragments arranged by size: 3758 1621 747 175 HaeII r_GCGC'y Cuts at: 1678 2046 2128 2135 2310 2396 5448 1678 Size: 368 82 7 175 86 3052 2531 Fragments arranged by size: 3052 2531 368 175 86 82 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3138 3170 3506 3538 4942 Size: 32 135 32 1566 32 336 32 1404 Cuts at: 4942 4974 5016 5048 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1566 1404 336 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3645 3891 669 Size: 988 281 1707 246 3079 Fragments arranged by size: 3079 1707 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6301 HpaI GTT'AAC Cuts at: 3891 3891 Size: 6301 KasI G'GCGC_C Cuts at: 2124 2306 2124 Size: 182 6119 KpnI G_GTAC'C Cuts at: 3626 3626 Size: 6301 MfeI C'AATT_G Cuts at: 3900 3900 Size: 6301 MluI A'CGCG_T Cuts at: 6177 6177 Size: 6301 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2815 3238 5415 5599 1305 Size: 1510 423 2177 184 2007 Fragments arranged by size: 2177 2007 1510 423 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2528 3563 10 Size: 54 1425 343 696 1035 2748 Fragments arranged by size: 2748 1425 1035 696 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2867 3233 3383 3386 4259 4618 4777 Size: 648 1701 366 150 3 873 359 159 Cuts at: 4777 518 Size: 2042 Fragments arranged by size: 2042 1701 873 648 366 359 159 150 3 MspA1I CmG'CkG Cuts at: 1606 2087 2281 2328 3173 3278 3551 3717 4394 Size: 481 194 47 845 105 273 166 677 Cuts at: 4394 5542 5787 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 845 677 481 273 245 194 166 105 47 NarI GG'CG_CC Cuts at: 2125 2307 2125 Size: 182 6119 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4810 Fragments arranged by size: 4810 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6301 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6301 NotI GC'GGCC_GC Cuts at: 3748 3748 Size: 6301 NspI r_CATG'y Cuts at: 1132 1229 2260 3659 5204 1132 Size: 97 1031 1399 1545 2229 Fragments arranged by size: 2229 1545 1399 1031 97 PasI CC'CwG_GG Cuts at: 1348 3409 1348 Size: 2061 4240 PciI A'CATG_T Cuts at: 5200 5200 Size: 6301 PflMI CCAn_nnn'nTGG Cuts at: 1108 2134 3166 1108 Size: 1026 1032 4243 Fragments arranged by size: 4243 1032 1026 PfoI T'CCnGG_A Cuts at: 2792 3306 2792 Size: 514 5787 PmeI GTTT'AAAC Cuts at: 3605 3605 Size: 6301 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2597 2629 2922 2954 4370 4402 5883 5915 2597 Size: 32 293 32 1416 32 1481 32 2983 Fragments arranged by size: 2983 1481 1416 293 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3286 3286 Size: 6301 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6301 PsiI TTA'TAA Cuts at: 2589 3871 2589 Size: 1282 5019 PspOMI G'GGCC_C Cuts at: 2066 2066 Size: 6301 PstI C_TGCA'G Cuts at: 830 2294 3653 830 Size: 1464 1359 3478 Fragments arranged by size: 3478 1464 1359 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6301 PvuII CAG'CTG Cuts at: 2328 3278 2328 Size: 950 5351 SacI G_AGCT'C Cuts at: 2077 3207 2077 Size: 1130 5171 SacII CC_GC'GG Cuts at: 2088 3174 2088 Size: 1086 5215 SalI G'TCGA_C Cuts at: 1936 3643 1936 Size: 1707 4594 SapI GCTCTTCn'nnn_ Cuts at: 2006 2006 Size: 6301 SbfI CC_TGCA'GG Cuts at: 3653 3653 Size: 6301 ScaI AGT'ACT Cuts at: 4549 6261 4549 Size: 1712 4589 SfcI C'TryA_G Cuts at: 826 1045 2290 3649 4784 5465 5656 826 Size: 219 1245 1359 1135 681 191 1471 Fragments arranged by size: 1471 1359 1245 1135 681 219 191 SfoI GGC'GCC Cuts at: 2126 2308 2126 Size: 182 6119 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6301 SmaI CCC'GGG Cuts at: 1872 3628 1872 Size: 1756 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4405 5306 5568 820 Size: 197 926 2462 901 262 1553 Fragments arranged by size: 2462 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2574 2809 4092 493 Size: 2081 235 1283 2702 Fragments arranged by size: 2702 2081 1283 235 SpeI A'CTAG_T Cuts at: 152 152 Size: 6301 SphI G_CATG'C Cuts at: 1132 2260 3659 1132 Size: 1128 1399 3774 Fragments arranged by size: 3774 1399 1128 SspI AAT'ATT Cuts at: 5 52 4225 5 Size: 47 4173 2081 Fragments arranged by size: 4173 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6301 StyI C'CwwG_G Cuts at: 513 1065 2004 2406 3585 3740 513 Size: 552 939 402 1179 155 3074 Fragments arranged by size: 3074 1179 939 552 402 155 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3401 4328 4345 4498 4683 5128 325 Size: 1255 1821 927 17 153 185 445 1498 Fragments arranged by size: 1821 1498 1255 927 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4547 6259 96 Size: 274 80 33 51 1443 2570 1712 138 Fragments arranged by size: 2570 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6261 6293 6261 Size: 32 6269 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6301 XbaI T'CTAG_A Cuts at: 3637 3637 Size: 6301 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2503 2924 3136 3548 2503 Size: 421 212 412 5256 Fragments arranged by size: 5256 421 412 212 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6301 XmaI C'CCGG_G Cuts at: 1870 3626 1870 Size: 1756 4545 XmnI GAAnn'nnTTC Cuts at: 4430 4430 Size: 6301 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4109 276 Size: 53 83 186 936 2575 2468 Fragments arranged by size: 2575 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BfrBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI