(Linear) (Six-base) MAPSORT of: KIEE4509.seq Check: 1935 from: 1 to: 1596 KIEE4509.orf With 182 enzymes: SgfI * March 22, 2012 18:14 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 427 1596 Size: 427 1169 AfeI AGC'GCT Cuts at: 0 39 128 1596 Size: 39 89 1468 Fragments arranged by size: 1468 89 39 AleI CACnn'nnGTG Cuts at: 0 1378 1381 1596 Size: 1378 3 215 Fragments arranged by size: 1378 215 3 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 117 1481 1596 Size: 117 1364 115 Fragments arranged by size: 1364 117 115 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 917 949 1596 Size: 917 32 647 Fragments arranged by size: 917 647 32 ApaI G_GGCC'C Cuts at: 0 65 1596 Size: 65 1531 AvaI C'yCGr_G Cuts at: 0 328 471 1596 Size: 328 143 1125 Fragments arranged by size: 1125 328 143 BamHI G'GATC_C Cuts at: 0 424 1596 Size: 424 1172 BanI G'GyrC_C Cuts at: 0 119 301 1596 Size: 119 182 1295 Fragments arranged by size: 1295 182 119 BanII G_rGCy'C Cuts at: 0 65 72 194 1202 1596 Size: 65 7 122 1008 394 Fragments arranged by size: 1008 394 122 65 7 BbeI G_GCGC'C Cuts at: 0 123 305 1596 Size: 123 182 1291 Fragments arranged by size: 1291 182 123 BbsI GAAGACnn'nnnn_ Cuts at: 0 740 1596 Size: 740 856 BbvCI CC'TCA_GC Cuts at: 0 1469 1596 Size: 1469 127 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 333 367 759 793 1596 Size: 333 34 392 34 803 Fragments arranged by size: 803 392 333 34 34 BclI T'GATC_A Cuts at: 0 263 1596 Size: 263 1333 BlpI GC'TnA_GC Cuts at: 0 193 1035 1596 Size: 193 842 561 Fragments arranged by size: 842 561 193 Bme1580I G_kGCm'C Cuts at: 0 65 1566 1596 Size: 65 1501 30 Fragments arranged by size: 1501 65 30 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 754 1300 1596 Size: 754 546 296 Fragments arranged by size: 754 546 296 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 484 1014 1295 1324 1596 Size: 484 530 281 29 272 Fragments arranged by size: 530 484 281 272 29 Bpu10I CC'TnA_GC Cuts at: 0 41 199 1469 1596 Size: 41 158 1270 127 Fragments arranged by size: 1270 158 127 41 BsaI GGTCTCn'nnnn_ Cuts at: 0 10 1596 Size: 10 1586 BsaAI yAC'GTr Cuts at: 0 569 804 1596 Size: 569 235 792 Fragments arranged by size: 792 569 235 BsaHI Gr'CG_yC Cuts at: 0 120 302 333 1596 Size: 120 182 31 1263 Fragments arranged by size: 1263 182 120 31 BsaWI w'CCGG_w Cuts at: 0 427 1367 1596 Size: 427 940 229 Fragments arranged by size: 940 427 229 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 917 947 1596 Size: 917 30 649 Fragments arranged by size: 917 649 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 470 985 1522 1525 1596 Size: 470 515 537 3 71 Fragments arranged by size: 537 515 470 71 3 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1430 1596 Size: 1430 166 BsiEI CG_ry'CG Cuts at: 0 59 1596 Size: 59 1537 BsiHKAI G_wGCw'C Cuts at: 0 72 1202 1596 Size: 72 1130 394 Fragments arranged by size: 1130 394 72 BsiWI C'GTAC_G Cuts at: 0 565 1596 Size: 565 1031 BsmI GAATG_Cn' Cuts at: 0 1415 1596 Size: 1415 181 BsmBI CGTCTCn'nnnn_ Cuts at: 0 490 1596 Size: 490 1106 Bsp1286I G_dGCh'C Cuts at: 0 65 72 194 1202 1566 1596 Size: 65 7 122 1008 364 30 Fragments arranged by size: 1008 364 122 65 30 7 BspHI T'CATG_A Cuts at: 0 1326 1596 Size: 1326 270 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 201 1033 1091 1340 1596 Size: 201 832 58 249 256 Fragments arranged by size: 832 256 249 201 58 BsrDI GCAATG_nn' Cuts at: 0 1175 1289 1596 Size: 1175 114 307 Fragments arranged by size: 1175 307 114 BstEII G'GTnAC_C Cuts at: 0 213 870 1596 Size: 213 657 726 Fragments arranged by size: 726 657 213 BstXI CCAn_nnnn'nTGG Cuts at: 0 456 549 1302 1380 1596 Size: 456 93 753 78 216 Fragments arranged by size: 753 456 216 93 78 BstYI r'GATC_y Cuts at: 0 424 632 1596 Size: 424 208 964 Fragments arranged by size: 964 424 208 BtgI C'CryG_G Cuts at: 0 80 395 1166 1376 1553 1596 Size: 80 315 771 210 177 43 Fragments arranged by size: 771 315 210 177 80 43 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 330 1502 1596 Size: 330 1172 94 Fragments arranged by size: 1172 330 94 DraIII CAC_nnn'GTG Cuts at: 0 379 1596 Size: 379 1217 DrdI GACnn_nn'nnGTC Cuts at: 0 444 1596 Size: 444 1152 EaeI y'GGCC_r Cuts at: 0 56 521 1163 1556 1596 Size: 56 465 642 393 40 Fragments arranged by size: 642 465 393 56 40 EagI C'GGCC_G Cuts at: 0 56 1596 Size: 56 1540 EarI CTCTTCn'nnn_ Cuts at: 0 1 1064 1596 Size: 1 1063 532 Fragments arranged by size: 1063 532 1 EcoICRI GAG'CTC Cuts at: 0 70 1200 1596 Size: 70 1130 396 Fragments arranged by size: 1130 396 70 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 427 484 1014 1295 1324 1596 Size: 427 57 530 281 29 272 Fragments arranged by size: 530 427 281 272 57 29 EcoNI CCTnn'n_nnAGG Cuts at: 0 763 1596 Size: 763 833 EcoO109I rG'GnC_Cy Cuts at: 0 49 475 1281 1596 Size: 49 426 806 315 Fragments arranged by size: 806 426 315 49 FspI TGC'GCA Cuts at: 0 259 434 1181 1596 Size: 259 175 747 415 Fragments arranged by size: 747 415 259 175 HaeII r_GCGC'y Cuts at: 0 41 123 130 305 391 1596 Size: 41 82 7 175 86 1205 Fragments arranged by size: 1205 175 86 82 41 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1133 1165 1501 1533 1596 Size: 1133 32 336 32 63 Fragments arranged by size: 1133 336 63 32 32 KasI G'GCGC_C Cuts at: 0 119 301 1596 Size: 119 182 1295 Fragments arranged by size: 1295 182 119 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 810 1233 1596 Size: 810 423 363 Fragments arranged by size: 810 423 363 MscI TGG'CCA Cuts at: 0 523 1558 1596 Size: 523 1035 38 Fragments arranged by size: 1035 523 38 MslI CAynn'nnrTG Cuts at: 0 862 1228 1378 1381 1596 Size: 862 366 150 3 215 Fragments arranged by size: 862 366 215 150 3 MspA1I CmG'CkG Cuts at: 0 82 276 323 1168 1273 1546 1596 Size: 82 194 47 845 105 273 50 Fragments arranged by size: 845 273 194 105 82 50 47 NarI GG'CG_CC Cuts at: 0 120 302 1596 Size: 120 182 1294 Fragments arranged by size: 1294 182 120 NspI r_CATG'y Cuts at: 0 255 1596 Size: 255 1341 PasI CC'CwG_GG Cuts at: 0 1404 1596 Size: 1404 192 PflMI CCAn_nnn'nTGG Cuts at: 0 129 1161 1596 Size: 129 1032 435 Fragments arranged by size: 1032 435 129 PfoI T'CCnGG_A Cuts at: 0 787 1301 1596 Size: 787 514 295 Fragments arranged by size: 787 514 295 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 592 624 917 949 1596 Size: 592 32 293 32 647 Fragments arranged by size: 647 592 293 32 32 PpuMI rG'GwC_Cy Cuts at: 0 1281 1596 Size: 1281 315 PsiI TTA'TAA Cuts at: 0 584 1596 Size: 584 1012 PspOMI G'GGCC_C Cuts at: 0 61 1596 Size: 61 1535 PstI C_TGCA'G Cuts at: 0 289 1596 Size: 289 1307 PvuII CAG'CTG Cuts at: 0 323 1273 1596 Size: 323 950 323 Fragments arranged by size: 950 323 323 SacI G_AGCT'C Cuts at: 0 72 1202 1596 Size: 72 1130 394 Fragments arranged by size: 1130 394 72 SacII CC_GC'GG Cuts at: 0 83 1169 1596 Size: 83 1086 427 Fragments arranged by size: 1086 427 83 SapI GCTCTTCn'nnn_ Cuts at: 0 1 1596 Size: 1 1595 SfcI C'TryA_G Cuts at: 0 285 1596 Size: 285 1311 SfoI GGC'GCC Cuts at: 0 121 303 1596 Size: 121 182 1293 Fragments arranged by size: 1293 182 121 SnaBI TAC'GTA Cuts at: 0 569 804 1596 Size: 569 235 792 Fragments arranged by size: 792 569 235 SphI G_CATG'C Cuts at: 0 255 1596 Size: 255 1341 StyI C'CwwG_G Cuts at: 0 401 1580 1596 Size: 401 1179 16 Fragments arranged by size: 1179 401 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1396 1596 Size: 1396 200 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 498 919 1131 1543 1596 Size: 498 421 212 412 53 Fragments arranged by size: 498 421 412 212 53 Enzymes that do cut: AcuI AfeI AleI AlfI AloI ApaI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI BlpI Bme1580I BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BstEII BstXI BstYI BtgI BtgZI DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KasI MmeI MscI MslI MspA1I NarI NspI PasI PflMI PfoI PpiI PpuMI PsiI PspOMI PstI PvuII SacI SacII SapI SfcI SfoI SnaBI SphI StyI TaqII XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BciVI BfrBI BglI BglII BmgBI BmrI BmtI BpuEI BsaBI BseYI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtsI ClaI CspCI DraI EciI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI MfeI MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PshAI PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SpeI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI