################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AgeI AhdI Aor51HI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BamHI BbvCI BciVI BclI BfrI BglI BlnI BsaBI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DrdI Eam1105I Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI MamI MfeI MluNI MroI MscI MseI MslI MunI NcoI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfiI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 511 GACGTC GACGT^C AccI 1230 GTCTAC GT^[AC][GT]AC AfaI 1016 GTAC GT^AC AlwI 1422 GATCC GGATC(4/5) AocI 744 CCTGAGG CC^T[ACGT]AGG ApaI 217 GGGCCC GGGCC^C AscI 14 GGCGCGCC GG^CGCGCC BbrPI 1242 CACGTG CAC^GTG BcgI 658 GCATATGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 764 CTAG C^TAG BlpI 1306 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1306 GCTGAGC GC^T[ACGT]AGC BsaAI 1242 CACGTG [CT]AC^GT[GA] BsaI 985 GAGACC GGTCTC(1/5) BseRI 964 CTCCTC GAGGAG(10/8) BsmBI 513 CGTCTC CGTCTC(1/5) BspLU11I 641 ACATGT A^CATGT BssSI 1440 CTCGTG CACGAG(-5/-1) BstAPI 84 GCAGCGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 744 CCTGAGG CC^T[ACGT]AGG CelII 1306 GCTGAGC GC^T[ACGT]AGC EagI 312 CGGCCG C^GGCCG EclXI 312 CGGCCG C^GGCCG Eco52I 312 CGGCCG C^GGCCG Eco81I 744 CCTGAGG CC^T[ACGT]AGG MaeI 764 CTAG C^TAG MluI 1114 ACGCGT A^CGCGT NdeI 659 CATATG CA^TATG NspI 641 ACATGT [GA]CATG^[CT] PflMI 1182 CCAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1242 CACGTG CAC^GTG PmlI 1242 CACGTG CAC^GTG RsaI 1016 GTAC GT^AC SacI 1087 GAGCTC GAGCT^C SgrAI 282 CGCCGGTG C[GA]^CCGG[CT]G SmlI 463 CTCAAG C^T[CT][GA]AG Van91I 1182 CCAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1182 CCAGCTTGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AflIII 641 ACATGT A^C[GA][CT]GT 1114 ACGCGT AlwNI 838 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG 1159 CAGGAGCTG AviII 269 TGCGCA TGC^GCA 1103 TGCGCA BglII 347 AGATCT A^GATCT 710 AGATCT BsiEI 312 CGGCCG CG[GA][CT]^CG 551 CGGTCG BspMI 1433 ACCTGC ACCTGC(4/8) 456 GCAGGT BssHII 15 GCGCGC G^CGCGC 45 GCGCGC BstXI 698 CCAGGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1034 CCACCAGACTGG DraIII 1120 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG 1293 CACACTGTG EarI 739 CTCTTC CTCTTC(1/4) 669 GAAGAG EcoT14I 752 CCAAGG C^C[AT][AT]GG 1061 CCAAGG FspI 269 TGCGCA TGC^GCA 1103 TGCGCA Ksp632I 739 CTCTTC CTCTTC(1/4) 669 GAAGAG NaeI 67 GCCGGC GCC^GGC 1004 GCCGGC NgoMIV 67 GCCGGC G^CCGGC 1004 GCCGGC NlaIII 442 CATG CATG^ 642 CATG PpuMI 458 AGGTCCT [GA]G^G[AT]CC[CT] 544 GGGACCT PshAI 142 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 546 GACCTCGGTC StyI 752 CCAAGG C^C[AT][AT]GG 1061 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AccII AciI AcyI AluI Alw44I ApaLI AspHI AvaI AvaII BanI BanII BbeI BbsI BbvI BcnI BmyI BpmI BpuAI BsaHI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KspI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIV PleI PstI PvuII SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI TaqI TseI Tsp45I TspRI TthHB8I XhoII