(Circular) (Six-base) MAPSORT of: pFN21AB5917.seq Check: 6220 from: 1 to: 6220 pFN21AB5917 6220 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2520 4030 278 Size: 53 83 186 936 984 1510 2468 Fragments arranged by size: 2468 1510 984 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3236 3563 1937 Size: 1299 327 4594 Fragments arranged by size: 4594 1299 327 Acc65I G'GTAC_C Cuts at: 3541 3541 Size: 6220 AclI AA'CG_TT Cuts at: 1563 4347 4720 1563 Size: 2784 373 3063 Fragments arranged by size: 3063 2784 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2573 2614 3357 3438 4283 5667 5935 1632 Size: 941 41 743 81 845 1384 268 1917 Fragments arranged by size: 1917 1384 941 845 743 268 81 41 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6023 AflIII A'CryG_T Cuts at: 1203 2646 3119 5119 6096 1203 Size: 1443 473 2000 977 1327 Fragments arranged by size: 2000 1443 1327 977 473 AgeI A'CCGG_T Cuts at: 5051 5051 Size: 6220 AhdI GACnn_n'nnGTC Cuts at: 4949 4949 Size: 6220 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6220 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2054 1184 Size: 870 5350 AlwNI CAG_nnn'CTG Cuts at: 2848 3169 3963 5535 2848 Size: 321 794 1572 3533 Fragments arranged by size: 3533 1572 794 321 ApaI G_GGCC'C Cuts at: 2226 2226 Size: 6220 ApaLI G'TGCA_C Cuts at: 2680 3050 3278 4277 5433 2680 Size: 370 228 999 1156 3467 Fragments arranged by size: 3467 1156 999 370 228 ApoI r'AATT_y Cuts at: 1493 3529 3755 3907 6016 1493 Size: 2036 226 152 2109 1697 Fragments arranged by size: 2109 2036 1697 226 152 AscI GG'CGCG_CC Cuts at: 2020 2020 Size: 6220 AseI AT'TA_AT Cuts at: 160 1032 4774 160 Size: 872 3742 1606 Fragments arranged by size: 3742 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 AvaI C'yCGr_G Cuts at: 1870 1943 2065 2156 2987 3327 3506 3545 1870 Size: 73 122 91 831 340 179 39 4545 Fragments arranged by size: 4545 831 340 179 122 91 73 39 BamHI G'GATC_C Cuts at: 3550 3917 3550 Size: 367 5853 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2201 2216 2259 3383 3541 Size: 325 217 627 414 15 43 1124 158 Cuts at: 3541 4996 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1124 627 414 325 217 158 43 15 BanII G_rGCy'C Cuts at: 1391 2066 2135 2163 2187 2226 2703 3074 3096 Size: 675 69 28 24 39 477 371 22 Cuts at: 3096 3539 1391 Size: 443 4072 Fragments arranged by size: 4072 675 477 443 371 69 39 28 24 22 BbeI G_GCGC'C Cuts at: 2205 2220 2263 2205 Size: 15 43 6162 Fragments arranged by size: 6162 43 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2433 2480 3363 3457 928 Size: 971 534 47 883 94 3691 Fragments arranged by size: 3691 971 883 534 94 47 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2652 2686 4411 4445 1903 Size: 34 715 34 1725 34 3678 Fragments arranged by size: 3678 1725 715 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4112 5322 4112 Size: 1210 5010 BclI T'GATC_A Cuts at: 1548 1548 Size: 6220 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4831 136 Size: 107 122 71 631 605 3159 1525 Fragments arranged by size: 3159 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2352 2715 6215 2352 Size: 363 3500 2357 Fragments arranged by size: 3500 2357 363 BlpI GC'TnA_GC Cuts at: 3312 3637 3312 Size: 325 5895 Bme1580I G_kGCm'C Cuts at: 1790 2226 2684 2930 3054 3081 3119 3282 4281 Size: 436 458 246 124 27 38 163 999 Cuts at: 4281 5437 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 999 458 436 246 163 124 38 27 BmgBI CAC'GTC Cuts at: 1318 2119 1318 Size: 801 5419 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2913 3951 4909 Size: 416 53 83 399 24 1496 1038 958 Cuts at: 4909 442 Size: 1753 Fragments arranged by size: 1753 1496 1038 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6220 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2737 3353 2737 Size: 616 5604 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2063 2237 2516 2732 4880 1239 Size: 824 174 279 216 2148 2579 Fragments arranged by size: 2579 2148 824 279 216 174 Bpu10I CC'TnA_GC Cuts at: 3005 3005 Size: 6220 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2453 4345 5210 5508 2453 Size: 1892 865 298 3165 Fragments arranged by size: 3165 1892 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2984 4883 882 Size: 617 1485 1899 2219 Fragments arranged by size: 2219 1899 1485 617 BsaAI yAC'GTr Cuts at: 493 1204 3249 4011 493 Size: 711 2045 762 2702 Fragments arranged by size: 2702 2045 762 711 BsaBI GATnn'nnATC Cuts at: 6214 6214 Size: 6220 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2202 2217 2260 Size: 53 83 186 936 476 193 15 43 Cuts at: 2260 2286 2517 2819 3259 4027 4409 5044 275 Size: 26 231 302 440 768 382 635 1451 Fragments arranged by size: 1451 936 768 635 476 440 382 302 231 193 186 83 53 43 26 15 BsaWI w'CCGG_w Cuts at: 4653 5051 5325 5472 5883 4653 Size: 398 274 147 411 4990 Fragments arranged by size: 4990 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2489 2519 2690 2720 1178 Size: 30 165 30 1086 30 171 30 4678 Fragments arranged by size: 4678 1086 171 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2960 1186 Size: 1774 4446 BseYI C'CCAG_C Cuts at: 2296 2562 2870 5423 5919 2296 Size: 266 308 2553 496 2597 Fragments arranged by size: 2597 2553 496 308 266 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2480 3229 3245 3342 3425 1163 Size: 573 744 749 16 97 83 3958 Fragments arranged by size: 3958 749 744 573 97 83 16 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2320 2559 3670 4431 5459 1540 Size: 275 186 319 239 1111 761 1028 2301 Fragments arranged by size: 2301 1111 1028 761 319 275 239 186 BsiHKAI G_wGCw'C Cuts at: 2684 3054 3096 3282 3339 4281 4366 5437 2684 Size: 370 42 186 57 942 85 1071 3467 Fragments arranged by size: 3467 1071 942 370 186 85 57 42 BsmI GAATG_Cn' Cuts at: 3731 3824 3731 Size: 93 6127 BsmBI CGTCTCn'nnnn_ Cuts at: 2524 2524 Size: 6220 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2066 2135 2163 2187 2226 2684 2703 Size: 399 276 69 28 24 39 458 19 Cuts at: 2703 2930 3054 3074 3081 3096 3119 3282 3339 Size: 227 124 20 7 15 23 163 57 Cuts at: 3339 3539 4281 4366 5437 1391 Size: 200 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 458 399 276 227 200 163 124 85 69 57 39 28 24 23 20 19 15 7 BspEI T'CCGG_A Cuts at: 5883 5883 Size: 6220 BspHI T'CATG_A Cuts at: 4109 5839 4109 Size: 1730 4490 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2452 3447 3575 844 Size: 1608 995 128 3489 Fragments arranged by size: 3489 1608 995 128 BsrBI CCG'CTC Cuts at: 1394 1823 4107 1394 Size: 429 2284 3507 Fragments arranged by size: 3507 2284 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4715 4889 65 Size: 1183 3467 174 1396 Fragments arranged by size: 3467 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2072 2288 3009 4864 5051 1700 Size: 110 262 216 721 1855 187 2869 Fragments arranged by size: 2869 1855 721 262 216 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6220 BssHII G'CGCG_C Cuts at: 1925 2020 2050 1925 Size: 95 30 6095 Fragments arranged by size: 6095 95 30 BssSI C'ACGA_G Cuts at: 3445 4280 5292 3445 Size: 835 1012 4373 Fragments arranged by size: 4373 1012 835 BstAPI GCAn_nnn'nTGC Cuts at: 794 2095 794 Size: 1301 4919 BstBI TT'CG_AA Cuts at: 5024 5024 Size: 6220 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6220 BstXI CCAn_nnnn'nTGG Cuts at: 2710 3046 2710 Size: 336 5884 BstYI r'GATC_y Cuts at: 1972 2352 2715 3426 3550 3917 4302 4319 5771 Size: 380 363 711 124 367 385 17 1452 Cuts at: 5771 5857 5869 6215 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 711 385 380 367 363 346 124 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2750 2750 Size: 6220 BtgI C'CryG_G Cuts at: 513 1065 2004 2171 2205 2822 513 Size: 552 939 167 34 617 3911 Fragments arranged by size: 3911 939 617 552 167 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2098 3019 487 Size: 683 56 872 921 3688 Fragments arranged by size: 3688 921 872 683 56 BtsI GCAGTG_nn' Cuts at: 3736 4530 4550 3736 Size: 794 20 5406 Fragments arranged by size: 5406 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6220 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6185 DraI TTT'AAA Cuts at: 3524 3524 Size: 6220 DraIII CAC_nnn'GTG Cuts at: 3130 3303 3130 Size: 173 6047 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5227 809 Size: 507 3911 1802 Fragments arranged by size: 3911 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2317 3011 3489 Size: 54 1425 50 275 18 487 694 478 Cuts at: 3489 3516 3667 4556 6185 8 Size: 27 151 889 1629 43 Fragments arranged by size: 1629 1425 889 694 487 478 275 151 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 2317 3667 1537 Size: 275 505 1350 4090 Fragments arranged by size: 4090 1350 505 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2669 2750 4150 1353 Size: 1316 81 1400 3423 Fragments arranged by size: 3423 1400 1316 81 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2126 4807 5181 5327 387 Size: 1064 138 537 2681 374 146 1280 Fragments arranged by size: 2681 1280 1064 537 374 146 138 EcoICRI GAG'CTC Cuts at: 3094 3094 Size: 6220 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2063 2237 2516 2573 2614 2732 3357 Size: 393 431 174 279 57 41 118 625 Cuts at: 3357 3438 4283 4880 5667 5935 1239 Size: 81 845 597 787 268 1524 Fragments arranged by size: 1524 845 787 625 597 431 393 279 268 174 118 81 57 41 EcoO109I rG'GnC_Cy Cuts at: 2223 2386 2464 2550 3001 3059 2223 Size: 163 78 86 451 58 5384 Fragments arranged by size: 5384 451 163 86 78 58 EcoRI G'AATT_C Cuts at: 3529 3907 3529 Size: 378 5842 FspI TGC'GCA Cuts at: 2276 3110 4726 2276 Size: 834 1616 3770 Fragments arranged by size: 3770 1616 834 HaeII r_GCGC'y Cuts at: 1678 2169 2205 2220 2246 2263 5367 1678 Size: 491 36 15 26 17 3104 2531 Fragments arranged by size: 3104 2531 491 36 26 17 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2420 2452 3428 3460 4861 Size: 32 135 32 848 32 976 32 1401 Cuts at: 4861 4893 4935 4967 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1401 976 848 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3564 3810 669 Size: 988 281 1626 246 3079 Fragments arranged by size: 3079 1626 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6220 HpaI GTT'AAC Cuts at: 3810 3810 Size: 6220 KasI G'GCGC_C Cuts at: 2201 2216 2259 2201 Size: 15 43 6162 Fragments arranged by size: 6162 43 15 KpnI G_GTAC'C Cuts at: 3545 3545 Size: 6220 MfeI C'AATT_G Cuts at: 3819 3819 Size: 6220 MluI A'CGCG_T Cuts at: 3119 6096 3119 Size: 2977 3243 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5334 5518 1305 Size: 4029 184 2007 Fragments arranged by size: 4029 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4398 Fragments arranged by size: 4398 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4178 4537 4696 518 Size: 648 3012 359 159 2042 Fragments arranged by size: 3012 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2092 2173 2207 2233 2257 2372 2593 2824 Size: 486 81 34 26 24 115 221 231 Cuts at: 2824 2870 2914 3433 3636 4313 5461 5706 1606 Size: 46 44 519 203 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 519 486 245 231 221 203 115 81 46 44 34 26 24 NaeI GCC'GGC Cuts at: 2074 3011 2074 Size: 937 5283 NarI GG'CG_CC Cuts at: 2202 2217 2260 2202 Size: 15 43 6162 Fragments arranged by size: 6162 43 15 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4729 Fragments arranged by size: 4729 939 552 NdeI CA'TA_TG Cuts at: 387 2665 387 Size: 2278 3942 NgoMIV G'CCGG_C Cuts at: 2072 3009 2072 Size: 937 5283 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6220 NotI GC'GGCC_GC Cuts at: 3667 3667 Size: 6220 NspI r_CATG'y Cuts at: 1132 1229 2650 3578 5123 1132 Size: 97 1421 928 1545 2229 Fragments arranged by size: 2229 1545 1421 928 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6220 PciI A'CATG_T Cuts at: 2646 5119 2646 Size: 2473 3747 PflMI CCAn_nnn'nTGG Cuts at: 1108 3193 1108 Size: 2085 4135 PfoI T'CCnGG_A Cuts at: 3399 3399 Size: 6220 PmeI GTTT'AAAC Cuts at: 3524 3524 Size: 6220 PmlI CAC'GTG Cuts at: 3249 3249 Size: 6220 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2955 2987 4289 4321 5802 5834 2955 Size: 32 1302 32 1481 32 3341 Fragments arranged by size: 3341 1481 1302 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2464 2550 2464 Size: 86 6134 PshAI GACnn'nnGTC Cuts at: 1613 2151 2555 1613 Size: 538 404 5278 Fragments arranged by size: 5278 538 404 PsiI TTA'TAA Cuts at: 3790 3790 Size: 6220 PspOMI G'GGCC_C Cuts at: 2222 2222 Size: 6220 PstI C_TGCA'G Cuts at: 830 2844 3269 3366 3572 830 Size: 2014 425 97 206 3478 Fragments arranged by size: 3478 2014 425 206 97 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6220 PvuII CAG'CTG Cuts at: 2233 2372 2870 2914 3433 2233 Size: 139 498 44 519 5020 Fragments arranged by size: 5020 519 498 139 44 SacI G_AGCT'C Cuts at: 3096 3096 Size: 6220 SacII CC_GC'GG Cuts at: 2174 2208 2825 2174 Size: 34 617 5569 Fragments arranged by size: 5569 617 34 SalI G'TCGA_C Cuts at: 1936 3562 1936 Size: 1626 4594 SbfI CC_TGCA'GG Cuts at: 3572 3572 Size: 6220 ScaI AGT'ACT Cuts at: 4468 6180 4468 Size: 1712 4508 SfcI C'TryA_G Cuts at: 826 1045 2840 3237 3265 3362 3568 4703 5384 Size: 219 1795 397 28 97 206 1135 681 Cuts at: 5384 5575 826 Size: 191 1471 Fragments arranged by size: 1795 1471 1135 681 397 219 206 191 97 28 SfoI GGC'GCC Cuts at: 2203 2218 2261 2203 Size: 15 43 6162 Fragments arranged by size: 6162 43 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 SgrAI Cr'CCGG_yG Cuts at: 1700 2288 1700 Size: 588 5632 SmaI CCC'GGG Cuts at: 1872 3547 1872 Size: 1675 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2468 4324 5225 5487 820 Size: 197 926 525 1856 901 262 1553 Fragments arranged by size: 1856 1553 926 901 525 262 197 SnaBI TAC'GTA Cuts at: 493 4011 493 Size: 3518 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6220 SphI G_CATG'C Cuts at: 1132 3578 1132 Size: 2446 3774 SspI AAT'ATT Cuts at: 5 52 4144 5 Size: 47 4092 2081 Fragments arranged by size: 4092 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6220 StyI C'CwwG_G Cuts at: 513 1065 2004 2757 3066 3659 513 Size: 552 939 753 309 593 3074 Fragments arranged by size: 3074 939 753 593 552 309 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2545 4247 4264 4417 4602 5047 325 Size: 1255 965 1702 17 153 185 445 1498 Fragments arranged by size: 1702 1498 1255 965 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4466 6178 96 Size: 274 80 33 51 1443 2489 1712 138 Fragments arranged by size: 2489 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6180 6212 6180 Size: 32 6188 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6220 XbaI T'CTAG_A Cuts at: 3556 3556 Size: 6220 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3194 3194 Size: 6220 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6220 XmaI C'CCGG_G Cuts at: 1870 3545 1870 Size: 1675 4545 XmnI GAAnn'nnTTC Cuts at: 4349 4349 Size: 6220 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2518 4028 276 Size: 53 83 186 936 984 1510 2468 Fragments arranged by size: 2468 1510 984 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI