(Linear) (Six-base) MAPSORT of: KIBB5917.seq Check: 330 from: 1 to: 1515 KIBB5917.orf With 182 enzymes: SgfI * July 15, 2010 18:30 .. AatII G_ACGT'C Cuts at: 0 515 1515 Size: 515 1000 AccI GT'mk_AC Cuts at: 0 1231 1515 Size: 1231 284 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 568 609 1352 1433 1515 Size: 568 41 743 81 82 Fragments arranged by size: 743 568 82 81 41 AflIII A'CryG_T Cuts at: 0 641 1114 1515 Size: 641 473 401 Fragments arranged by size: 641 473 401 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 49 1515 Size: 49 1466 AlwNI CAG_nnn'CTG Cuts at: 0 843 1164 1515 Size: 843 321 351 Fragments arranged by size: 843 351 321 ApaI G_GGCC'C Cuts at: 0 221 1515 Size: 221 1294 ApaLI G'TGCA_C Cuts at: 0 675 1045 1273 1515 Size: 675 370 228 242 Fragments arranged by size: 675 370 242 228 AscI GG'CGCG_CC Cuts at: 0 15 1515 Size: 15 1500 AvaI C'yCGr_G Cuts at: 0 60 151 982 1322 1501 1515 Size: 60 91 831 340 179 14 Fragments arranged by size: 831 340 179 91 60 14 BanI G'GyrC_C Cuts at: 0 196 211 254 1378 1515 Size: 196 15 43 1124 137 Fragments arranged by size: 1124 196 137 43 15 BanII G_rGCy'C Cuts at: 0 61 130 158 182 221 698 1069 1091 Size: 61 69 28 24 39 477 371 22 Cuts at: 1091 1515 Size: 424 Fragments arranged by size: 477 424 371 69 61 39 28 24 22 BbeI G_GCGC'C Cuts at: 0 200 215 258 1515 Size: 200 15 43 1257 Fragments arranged by size: 1257 200 43 15 BbsI GAAGACnn'nnnn_ Cuts at: 0 428 475 1358 1452 1515 Size: 428 47 883 94 63 Fragments arranged by size: 883 428 94 63 47 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 647 681 1515 Size: 647 34 834 Fragments arranged by size: 834 647 34 BglII A'GATC_T Cuts at: 0 347 710 1515 Size: 347 363 805 Fragments arranged by size: 805 363 347 BlpI GC'TnA_GC Cuts at: 0 1307 1515 Size: 1307 208 Bme1580I G_kGCm'C Cuts at: 0 221 679 925 1049 1076 1114 1277 1515 Size: 221 458 246 124 27 38 163 238 Fragments arranged by size: 458 246 238 221 163 124 38 27 BmgBI CAC'GTC Cuts at: 0 114 1515 Size: 114 1401 BmrI ACTGGGnnnn_n' Cuts at: 0 908 1515 Size: 908 607 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 732 1348 1515 Size: 732 616 167 Fragments arranged by size: 732 616 167 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 58 232 511 727 1515 Size: 58 174 279 216 788 Fragments arranged by size: 788 279 216 174 58 Bpu10I CC'TnA_GC Cuts at: 0 1000 1515 Size: 1000 515 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 448 1515 Size: 448 1067 BsaI GGTCTCn'nnnn_ Cuts at: 0 979 1515 Size: 979 536 BsaAI yAC'GTr Cuts at: 0 1244 1515 Size: 1244 271 BsaHI Gr'CG_yC Cuts at: 0 4 197 212 255 281 512 814 1254 Size: 4 193 15 43 26 231 302 440 Cuts at: 1254 1515 Size: 261 Fragments arranged by size: 440 302 261 231 193 43 26 15 4 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 484 514 685 715 1515 Size: 484 30 171 30 800 Fragments arranged by size: 800 484 171 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 955 1515 Size: 955 560 BseYI C'CCAG_C Cuts at: 0 291 557 865 1515 Size: 291 266 308 650 Fragments arranged by size: 650 308 291 266 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 475 1224 1240 1337 1420 1515 Size: 475 749 16 97 83 95 Fragments arranged by size: 749 475 97 95 83 16 BsiEI CG_ry'CG Cuts at: 0 315 554 1515 Size: 315 239 961 Fragments arranged by size: 961 315 239 BsiHKAI G_wGCw'C Cuts at: 0 679 1049 1091 1277 1334 1515 Size: 679 370 42 186 57 181 Fragments arranged by size: 679 370 186 181 57 42 BsmBI CGTCTCn'nnnn_ Cuts at: 0 519 1515 Size: 519 996 Bsp1286I G_dGCh'C Cuts at: 0 61 130 158 182 221 679 698 925 Size: 61 69 28 24 39 458 19 227 Cuts at: 925 1049 1069 1076 1091 1114 1277 1334 1515 Size: 124 20 7 15 23 163 57 181 Fragments arranged by size: 458 227 181 163 124 69 61 57 39 28 24 23 20 19 15 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 447 1442 1515 Size: 447 995 73 Fragments arranged by size: 995 447 73 BsrFI r'CCGG_y Cuts at: 0 67 283 1004 1515 Size: 67 216 721 511 Fragments arranged by size: 721 511 216 67 BssHII G'CGCG_C Cuts at: 0 15 45 1515 Size: 15 30 1470 Fragments arranged by size: 1470 30 15 BssSI C'ACGA_G Cuts at: 0 1440 1515 Size: 1440 75 BstAPI GCAn_nnn'nTGC Cuts at: 0 90 1515 Size: 90 1425 BstXI CCAn_nnnn'nTGG Cuts at: 0 705 1041 1515 Size: 705 336 474 Fragments arranged by size: 705 474 336 BstYI r'GATC_y Cuts at: 0 347 710 1421 1515 Size: 347 363 711 94 Fragments arranged by size: 711 363 347 94 Bsu36I CC'TnA_GG Cuts at: 0 745 1515 Size: 745 770 BtgI C'CryG_G Cuts at: 0 166 200 817 1515 Size: 166 34 617 698 Fragments arranged by size: 698 617 166 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 93 1014 1515 Size: 93 921 501 Fragments arranged by size: 921 501 93 DraIII CAC_nnn'GTG Cuts at: 0 1125 1298 1515 Size: 1125 173 217 Fragments arranged by size: 1125 217 173 EaeI y'GGCC_r Cuts at: 0 312 1006 1484 1515 Size: 312 694 478 31 Fragments arranged by size: 694 478 312 31 EagI C'GGCC_G Cuts at: 0 312 1515 Size: 312 1203 EarI CTCTTCn'nnn_ Cuts at: 0 664 745 1515 Size: 664 81 770 Fragments arranged by size: 770 664 81 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 121 1515 Size: 121 1394 EcoICRI GAG'CTC Cuts at: 0 1089 1515 Size: 1089 426 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 58 232 511 568 609 727 1352 1433 Size: 58 174 279 57 41 118 625 81 Cuts at: 1433 1515 Size: 82 Fragments arranged by size: 625 279 174 118 82 81 58 57 41 EcoO109I rG'GnC_Cy Cuts at: 0 218 381 459 545 996 1054 1515 Size: 218 163 78 86 451 58 461 Fragments arranged by size: 461 451 218 163 86 78 58 FspI TGC'GCA Cuts at: 0 271 1105 1515 Size: 271 834 410 Fragments arranged by size: 834 410 271 HaeII r_GCGC'y Cuts at: 0 164 200 215 241 258 1515 Size: 164 36 15 26 17 1257 Fragments arranged by size: 1257 164 36 26 17 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 415 447 1423 1455 1515 Size: 415 32 976 32 60 Fragments arranged by size: 976 415 60 32 32 KasI G'GCGC_C Cuts at: 0 196 211 254 1515 Size: 196 15 43 1261 Fragments arranged by size: 1261 196 43 15 MluI A'CGCG_T Cuts at: 0 1114 1515 Size: 1114 401 MspA1I CmG'CkG Cuts at: 0 87 168 202 228 252 367 588 819 Size: 87 81 34 26 24 115 221 231 Cuts at: 819 865 909 1428 1515 Size: 46 44 519 87 Fragments arranged by size: 519 231 221 115 87 87 81 46 44 34 26 24 NaeI GCC'GGC Cuts at: 0 69 1006 1515 Size: 69 937 509 Fragments arranged by size: 937 509 69 NarI GG'CG_CC Cuts at: 0 197 212 255 1515 Size: 197 15 43 1260 Fragments arranged by size: 1260 197 43 15 NdeI CA'TA_TG Cuts at: 0 660 1515 Size: 660 855 NgoMIV G'CCGG_C Cuts at: 0 67 1004 1515 Size: 67 937 511 Fragments arranged by size: 937 511 67 NspI r_CATG'y Cuts at: 0 645 1515 Size: 645 870 PciI A'CATG_T Cuts at: 0 641 1515 Size: 641 874 PflMI CCAn_nnn'nTGG Cuts at: 0 1188 1515 Size: 1188 327 PfoI T'CCnGG_A Cuts at: 0 1394 1515 Size: 1394 121 PmlI CAC'GTG Cuts at: 0 1244 1515 Size: 1244 271 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 950 982 1515 Size: 950 32 533 Fragments arranged by size: 950 533 32 PpuMI rG'GwC_Cy Cuts at: 0 459 545 1515 Size: 459 86 970 Fragments arranged by size: 970 459 86 PshAI GACnn'nnGTC Cuts at: 0 146 550 1515 Size: 146 404 965 Fragments arranged by size: 965 404 146 PspOMI G'GGCC_C Cuts at: 0 217 1515 Size: 217 1298 PstI C_TGCA'G Cuts at: 0 839 1264 1361 1515 Size: 839 425 97 154 Fragments arranged by size: 839 425 154 97 PvuII CAG'CTG Cuts at: 0 228 367 865 909 1428 1515 Size: 228 139 498 44 519 87 Fragments arranged by size: 519 498 228 139 87 44 SacI G_AGCT'C Cuts at: 0 1091 1515 Size: 1091 424 SacII CC_GC'GG Cuts at: 0 169 203 820 1515 Size: 169 34 617 695 Fragments arranged by size: 695 617 169 34 SfcI C'TryA_G Cuts at: 0 835 1232 1260 1357 1515 Size: 835 397 28 97 158 Fragments arranged by size: 835 397 158 97 28 SfoI GGC'GCC Cuts at: 0 198 213 256 1515 Size: 198 15 43 1259 Fragments arranged by size: 1259 198 43 15 SgrAI Cr'CCGG_yG Cuts at: 0 283 1515 Size: 283 1232 SmlI C'TyrA_G Cuts at: 0 463 1515 Size: 463 1052 StyI C'CwwG_G Cuts at: 0 752 1061 1515 Size: 752 309 454 Fragments arranged by size: 752 454 309 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 540 1515 Size: 540 975 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1189 1515 Size: 1189 326 ZraI GAC'GTC Cuts at: 0 513 1515 Size: 513 1002 Enzymes that do cut: AatII AccI AcuI AflIII AlfI AlwNI ApaI ApaLI AscI AvaI BanI BanII BbeI BbsI BcgI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FspI HaeII Hin4I KasI MluI MspA1I NaeI NarI NdeI NgoMIV NspI PciI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PstI PvuII SacI SacII SfcI SfoI SgrAI SmlI StyI TaqII XcmI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AgeI AhdI AleI AloI ApoI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglI BmtI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI BstBI BstEII BstZ17I BtsI ClaI CspCI DraI DrdI EcoNI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI MfeI MmeI MscI MslI NcoI NheI NotI NruI NsiI PacI PasI PmeI PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI