################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AatII Acc65I AccII AclI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspI BcgI BclI BfrI BglII BsaBI BsiEI BsiWI BspDI BspHI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Cfr10I ClaI CpoI DraI DraIII EagI EclXI Eco47III Eco52I EcoRV EcoT22I FbaI FseI HgaI HindIII HpaI KpnI KspI MamI MfeI MluI MunI MvnI NaeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI SspI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccIII 3770 TCCGGA T^CCGGA AcyI 2907 GGCGCC G[GA]^CG[CT]C AflIII 14 ACGTGT A^C[GA][CT]GT AhdI 544 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 544 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 3341 TGCGCA TGC^GCA AvrII 3880 CCTAGG C^CTAGG BbeI 2907 GGCGCC GGCGC^C BbrPI 13 CACGTG CAC^GTG BlnI 3880 CCTAGG C^CTAGG BsaAI 13 CACGTG [CT]AC^GT[GA] BsaHI 2907 GGCGCC G[GA]^CG[CT]C BseAI 3770 TCCGGA T^CCGGA Bsp1407I 53 TGTACA T^GTACA BspEI 3770 TCCGGA T^CCGGA BsrGI 53 TGTACA T^GTACA BstEII 2442 GGTGACC G^GT[ACGT]ACC BstPI 2442 GGTGACC G^GT[ACGT]ACC DrdI 2423 GACAGCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 544 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 2442 GGTGACC G^GT[ACGT]ACC EcoRI 3366 GAATTC G^AATTC FspI 3341 TGCGCA TGC^GCA Hin1I 2907 GGCGCC G[GA]^CG[CT]C KasI 2907 GGCGCC G^GCGCC MroI 3770 TCCGGA T^CCGGA MseI 680 TTAA T^TAA MslI 2377 CATTCGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 2907 GGCGCC GG^CGCC NheI 2596 GCTAGC G^CTAGC PmaCI 13 CACGTG CAC^GTG PmlI 13 CACGTG CAC^GTG SacI 256 GAGCTC GAGCT^C SapI 1086 GAAGAGC GCTCTTC(1/4) SfoI 2907 GGCGCC GGC^GCC SmaI 440 CCCGGG CCC^GGG SspBI 53 TGTACA T^GTACA TaqI 63 TCGA T^CGA Tru9I 680 TTAA T^TAA TthHB8I 63 TCGA T^CGA XmaCI 440 CCCGGG C^CCGGG XmaI 440 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccI 1520 GTCTAC GT^[AC][GT]AC 2271 GTCTAC AcsI 138 GAATTT [GA]^AATT[CT] 3366 GAATTC ApoI 138 GAATTT [GA]^AATT[CT] 3366 GAATTC BamHI 3129 GGATCC G^GATCC 3492 GGATCC BciVI 2846 GTATCC GTATCC(6/5) 3620 GTATCC BglI 3347 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3538 GCCTACCAGGC BlpI 2775 GCTGAGC GC^T[ACGT]AGC 3459 GCTCAGC Bpu1102I 2775 GCTGAGC GC^T[ACGT]AGC 3459 GCTCAGC BsaI 1273 GAGACC GGTCTC(1/5) 3728 GAGACC BsaWI 1142 ACCGGA [AT]^CCGG[AT] 3770 TCCGGA BsmBI 2388 CGTCTC CGTCTC(1/5) 3186 CGTCTC CelII 2775 GCTGAGC GC^T[ACGT]AGC 3459 GCTCAGC EarI 1086 GAAGAG CTCTTC(1/4) 2266 GAAGAG HaeII 116 GGCGCT [GA]GCGC^[CT] 2907 GGCGCC HincII 2517 GTTGAC GT[CT]^[GA]AC 3228 GTTGAC HindII 2517 GTTGAC GT[CT]^[GA]AC 3228 GTTGAC Ksp632I 1086 GAAGAG CTCTTC(1/4) 2266 GAAGAG MaeII 14 ACGT A^CGT 2357 ACGT NdeI 193 CATATG CA^TATG 1992 CATATG SbfI 690 CCTGCAGG CCTGCA^GG 1659 CCTGCAGG SexAI 299 ACCAGGT A^CC[AT]GGT 2526 ACCAGGT Sse8387I 690 CCTGCAGG CCTGCA^GG 1659 CCTGCAGG ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AocI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TfiI TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII