(Linear) (Six-base) MAPSORT of: KIEE1427.seq Check: 2757 from: 1 to: 3945 KIEE1427.orf With 182 enzymes: SgfI * July 15, 2010 18:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1274 1653 3945 Size: 1274 379 2292 Fragments arranged by size: 2292 1274 379 AccI GT'mk_AC Cuts at: 0 1521 2272 3945 Size: 1521 751 1673 Fragments arranged by size: 1673 1521 751 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 115 314 826 1321 2093 2297 2884 3683 Size: 115 199 512 495 772 204 587 799 Cuts at: 3683 3945 Size: 262 Fragments arranged by size: 799 772 587 512 495 262 204 199 115 AflIII A'CryG_T Cuts at: 0 14 3945 Size: 14 3931 AhdI GACnn_n'nnGTC Cuts at: 0 549 3945 Size: 549 3396 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2010 3442 3945 Size: 2010 1432 503 Fragments arranged by size: 2010 1432 503 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 830 862 1154 1186 3945 Size: 830 32 292 32 2759 Fragments arranged by size: 2759 830 292 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 674 699 726 967 1111 1266 1383 1590 Size: 674 25 27 241 144 155 117 207 Cuts at: 1590 1938 3166 3225 3807 3864 3945 Size: 348 1228 59 582 57 81 Fragments arranged by size: 1228 674 582 348 241 207 155 144 117 81 59 57 27 25 ApoI r'AATT_y Cuts at: 0 138 3366 3945 Size: 138 3228 579 Fragments arranged by size: 3228 579 138 AvaI C'yCGr_G Cuts at: 0 440 1030 1133 2674 3945 Size: 440 590 103 1541 1271 Fragments arranged by size: 1541 1271 590 440 103 AvrII C'CTAG_G Cuts at: 0 3880 3945 Size: 3880 65 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 3796 3829 3945 Size: 3796 33 116 Fragments arranged by size: 3796 116 33 BamHI G'GATC_C Cuts at: 0 3129 3492 3945 Size: 3129 363 453 Fragments arranged by size: 3129 453 363 BanI G'GyrC_C Cuts at: 0 586 1119 2645 2907 3820 3945 Size: 586 533 1526 262 913 125 Fragments arranged by size: 1526 913 586 533 262 125 BanII G_rGCy'C Cuts at: 0 260 1868 1920 2207 2810 3945 Size: 260 1608 52 287 603 1135 Fragments arranged by size: 1608 1135 603 287 260 52 BbeI G_GCGC'C Cuts at: 0 2911 3945 Size: 2911 1034 BbsI GAAGACnn'nnnn_ Cuts at: 0 302 1435 2081 2182 3945 Size: 302 1133 646 101 1763 Fragments arranged by size: 1763 1133 646 302 101 BbvCI CC'TCA_GC Cuts at: 0 133 248 929 3945 Size: 133 115 681 3016 Fragments arranged by size: 3016 681 133 115 BciVI GTATCCnnnnn_n' Cuts at: 0 2857 3631 3945 Size: 2857 774 314 Fragments arranged by size: 2857 774 314 BglI GCCn_nnn'nGGC Cuts at: 0 3353 3544 3945 Size: 3353 191 401 Fragments arranged by size: 3353 401 191 BlpI GC'TnA_GC Cuts at: 0 2776 3460 3945 Size: 2776 684 485 Fragments arranged by size: 2776 684 485 Bme1580I G_kGCm'C Cuts at: 0 1124 3381 3438 3584 3945 Size: 1124 2257 57 146 361 Fragments arranged by size: 2257 1124 361 146 57 BmrI ACTGGGnnnn_n' Cuts at: 0 315 2805 3578 3945 Size: 315 2490 773 367 Fragments arranged by size: 2490 773 367 315 BmtI G_CTAG'C Cuts at: 0 2600 3945 Size: 2600 1345 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1140 3945 Size: 1140 2805 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 488 1133 1391 1434 1537 1654 1812 1985 Size: 488 645 258 43 103 117 158 173 Cuts at: 1985 2440 3945 Size: 455 1505 Fragments arranged by size: 1505 645 488 455 258 173 158 117 103 43 Bpu10I CC'TnA_GC Cuts at: 0 80 133 248 929 1435 2089 2729 2821 Size: 80 53 115 681 506 654 640 92 Cuts at: 2821 3945 Size: 1124 Fragments arranged by size: 1124 681 654 640 506 115 92 80 53 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 622 994 2128 3945 Size: 622 372 1134 1817 Fragments arranged by size: 1817 1134 622 372 BsaI GGTCTCn'nnnn_ Cuts at: 0 1267 3722 3945 Size: 1267 2455 223 Fragments arranged by size: 2455 1267 223 BsaAI yAC'GTr Cuts at: 0 15 3945 Size: 15 3930 BsaHI Gr'CG_yC Cuts at: 0 2908 3945 Size: 2908 1037 BsaWI w'CCGG_w Cuts at: 0 1142 3770 3945 Size: 1142 2628 175 Fragments arranged by size: 2628 1142 175 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2638 2668 2797 2827 3392 3422 3945 Size: 2638 30 129 30 565 30 523 Fragments arranged by size: 2638 565 523 129 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 385 532 946 1031 1192 1249 1298 1653 Size: 385 147 414 85 161 57 49 355 Cuts at: 1653 2566 2692 3691 3945 Size: 913 126 999 254 Fragments arranged by size: 999 913 414 385 355 254 161 147 126 85 57 49 BseYI C'CCAG_C Cuts at: 0 1094 1194 2024 2834 2881 3280 3593 3701 Size: 1094 100 830 810 47 399 313 108 Cuts at: 3701 3734 3945 Size: 33 211 Fragments arranged by size: 1094 830 810 399 313 211 108 100 47 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1112 2119 2423 2672 3528 3945 Size: 1112 1007 304 249 856 417 Fragments arranged by size: 1112 1007 856 417 304 249 BsiHKAI G_wGCw'C Cuts at: 0 81 260 454 1210 2945 3945 Size: 81 179 194 756 1735 1000 Fragments arranged by size: 1735 1000 756 194 179 81 BsmI GAATG_Cn' Cuts at: 0 348 1168 1423 3945 Size: 348 820 255 2522 Fragments arranged by size: 2522 820 348 255 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2394 3192 3945 Size: 2394 798 753 Fragments arranged by size: 2394 798 753 Bsp1286I G_dGCh'C Cuts at: 0 81 260 454 1124 1210 1868 1920 2207 Size: 81 179 194 670 86 658 52 287 Cuts at: 2207 2810 2945 3381 3438 3584 3945 Size: 603 135 436 57 146 361 Fragments arranged by size: 670 658 603 436 361 287 194 179 146 135 86 81 57 52 BspEI T'CCGG_A Cuts at: 0 3770 3945 Size: 3770 175 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 975 1274 1653 1839 2031 2333 3045 3570 Size: 975 299 379 186 192 302 712 525 Cuts at: 3570 3945 Size: 375 Fragments arranged by size: 975 712 525 379 375 302 299 192 186 BsrDI GCAATG_nn' Cuts at: 0 311 3537 3636 3761 3945 Size: 311 3226 99 125 184 Fragments arranged by size: 3226 311 184 125 99 BsrGI T'GTAC_A Cuts at: 0 53 3945 Size: 53 3892 BstAPI GCAn_nnn'nTGC Cuts at: 0 699 1266 1743 3945 Size: 699 567 477 2202 Fragments arranged by size: 2202 699 567 477 BstEII G'GTnAC_C Cuts at: 0 2442 3945 Size: 2442 1503 BstXI CCAn_nnnn'nTGG Cuts at: 0 462 999 3601 3945 Size: 462 537 2602 344 Fragments arranged by size: 2602 537 462 344 BstYI r'GATC_y Cuts at: 0 266 1067 1379 3129 3492 3606 3945 Size: 266 801 312 1750 363 114 339 Fragments arranged by size: 1750 801 363 339 312 266 114 Bsu36I CC'TnA_GG Cuts at: 0 1043 2665 3022 3611 3925 3945 Size: 1043 1622 357 589 314 20 Fragments arranged by size: 1622 1043 589 357 314 20 BtgI C'CryG_G Cuts at: 0 715 1787 2462 2657 2786 3945 Size: 715 1072 675 195 129 1159 Fragments arranged by size: 1159 1072 715 675 195 129 BtsI GCAGTG_nn' Cuts at: 0 1464 1936 1997 2358 3436 3516 3584 3862 Size: 1464 472 61 361 1078 80 68 278 Cuts at: 3862 3900 3945 Size: 38 45 Fragments arranged by size: 1464 1078 472 361 278 80 68 61 45 38 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 390 425 3945 Size: 390 35 3520 Fragments arranged by size: 3520 390 35 DrdI GACnn_nn'nnGTC Cuts at: 0 2429 3945 Size: 2429 1516 EaeI y'GGCC_r Cuts at: 0 733 1061 2783 2789 3014 3945 Size: 733 328 1722 6 225 931 Fragments arranged by size: 1722 931 733 328 225 6 EarI CTCTTCn'nnn_ Cuts at: 0 1081 2261 3945 Size: 1081 1180 1684 Fragments arranged by size: 1684 1180 1081 EcoICRI GAG'CTC Cuts at: 0 258 3945 Size: 258 3687 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 115 314 488 826 1133 1321 1391 1434 Size: 115 199 174 338 307 188 70 43 Cuts at: 1434 1537 1654 1812 1985 2093 2297 2440 2884 Size: 103 117 158 173 108 204 143 444 Cuts at: 2884 3683 3945 Size: 799 262 Fragments arranged by size: 799 444 338 307 262 204 199 188 174 173 158 143 117 115 108 103 70 43 EcoNI CCTnn'n_nnAGG Cuts at: 0 400 2143 3781 3945 Size: 400 1743 1638 164 Fragments arranged by size: 1743 1638 400 164 EcoO109I rG'GnC_Cy Cuts at: 0 642 809 846 964 1431 1574 1587 2178 Size: 642 167 37 118 467 143 13 591 Cuts at: 2178 3270 3559 3658 3945 Size: 1092 289 99 287 Fragments arranged by size: 1092 642 591 467 289 287 167 143 118 99 37 13 EcoRI G'AATT_C Cuts at: 0 3366 3945 Size: 3366 579 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2719 3945 Size: 2719 1226 FspI TGC'GCA Cuts at: 0 3343 3945 Size: 3343 602 HaeII r_GCGC'y Cuts at: 0 120 2911 3945 Size: 120 2791 1034 Fragments arranged by size: 2791 1034 120 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 535 567 1364 1396 2668 2700 3121 3153 Size: 535 32 797 32 1272 32 421 32 Cuts at: 3153 3229 3261 3392 3424 3820 3852 3945 Size: 76 32 131 32 396 32 93 Fragments arranged by size: 1272 797 535 421 396 131 93 76 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 2519 3230 3945 Size: 2519 711 715 Fragments arranged by size: 2519 715 711 KasI G'GCGC_C Cuts at: 0 2907 3945 Size: 2907 1038 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 3260 3435 3945 Size: 3260 175 510 Fragments arranged by size: 3260 510 175 MscI TGG'CCA Cuts at: 0 735 2785 2791 3945 Size: 735 2050 6 1154 Fragments arranged by size: 2050 1154 735 6 MslI CAynn'nnrTG Cuts at: 0 2381 3945 Size: 2381 1564 MspA1I CmG'CkG Cuts at: 0 541 621 1094 1203 1359 1454 3574 3597 Size: 541 80 473 109 156 95 2120 23 Cuts at: 3597 3705 3945 Size: 108 240 Fragments arranged by size: 2120 541 473 240 156 109 108 95 80 23 NarI GG'CG_CC Cuts at: 0 2908 3945 Size: 2908 1037 NcoI C'CATG_G Cuts at: 0 1787 2462 2657 2786 3945 Size: 1787 675 195 129 1159 Fragments arranged by size: 1787 1159 675 195 129 NdeI CA'TA_TG Cuts at: 0 194 1993 3945 Size: 194 1799 1952 Fragments arranged by size: 1952 1799 194 NheI G'CTAG_C Cuts at: 0 2596 3945 Size: 2596 1349 NspI r_CATG'y Cuts at: 0 2386 2491 3725 3945 Size: 2386 105 1234 220 Fragments arranged by size: 2386 1234 220 105 PasI CC'CwG_GG Cuts at: 0 2757 3816 3945 Size: 2757 1059 129 Fragments arranged by size: 2757 1059 129 PflMI CCAn_nnn'nTGG Cuts at: 0 18 873 2463 2468 2763 3945 Size: 18 855 1590 5 295 1182 Fragments arranged by size: 1590 1182 855 295 18 5 PmlI CAC'GTG Cuts at: 0 15 3945 Size: 15 3930 PpuMI rG'GwC_Cy Cuts at: 0 642 809 846 964 1431 1574 1587 3270 Size: 642 167 37 118 467 143 13 1683 Cuts at: 3270 3559 3658 3945 Size: 289 99 287 Fragments arranged by size: 1683 642 467 289 287 167 143 118 99 37 13 PstI C_TGCA'G Cuts at: 0 695 1459 1664 2117 2481 2508 3056 3443 Size: 695 764 205 453 364 27 548 387 Cuts at: 3443 3579 3907 3945 Size: 136 328 38 Fragments arranged by size: 764 695 548 453 387 364 328 205 136 38 27 PvuII CAG'CTG Cuts at: 0 621 1094 1359 1454 3574 3597 3945 Size: 621 473 265 95 2120 23 348 Fragments arranged by size: 2120 621 473 348 265 95 23 SacI G_AGCT'C Cuts at: 0 260 3945 Size: 260 3685 SanDI GG'GwC_CC Cuts at: 0 809 3945 Size: 809 3136 SapI GCTCTTCn'nnn_ Cuts at: 0 1081 3945 Size: 1081 2864 SbfI CC_TGCA'GG Cuts at: 0 695 1664 3945 Size: 695 969 2281 Fragments arranged by size: 2281 969 695 SexAI A'CCwGG_T Cuts at: 0 299 2526 3945 Size: 299 2227 1419 Fragments arranged by size: 2227 1419 299 SfcI C'TryA_G Cuts at: 0 691 1455 1660 2113 2477 2504 3052 3439 Size: 691 764 205 453 364 27 548 387 Cuts at: 3439 3575 3903 3945 Size: 136 328 42 Fragments arranged by size: 764 691 548 453 387 364 328 205 136 42 27 SfoI GGC'GCC Cuts at: 0 2909 3945 Size: 2909 1036 SmaI CCC'GGG Cuts at: 0 442 3945 Size: 442 3503 SmlI C'TyrA_G Cuts at: 0 637 973 2143 3945 Size: 637 336 1170 1802 Fragments arranged by size: 1802 1170 637 336 SrfI GCCC'GGGC Cuts at: 0 442 3945 Size: 442 3503 StuI AGG'CCT Cuts at: 0 913 1609 3885 3945 Size: 913 696 2276 60 Fragments arranged by size: 2276 913 696 60 StyI C'CwwG_G Cuts at: 0 401 881 1787 1911 2033 2213 2462 2657 Size: 401 480 906 124 122 180 249 195 Cuts at: 2657 2786 3880 3945 Size: 129 1094 65 Fragments arranged by size: 1094 906 480 401 249 195 180 129 124 122 65 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 3374 3808 3945 Size: 3374 434 137 Fragments arranged by size: 3374 434 137 TatI w'GTAC_w Cuts at: 0 53 338 3945 Size: 53 285 3607 Fragments arranged by size: 3607 285 53 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2276 2308 2638 2670 3945 Size: 2276 32 330 32 1275 Fragments arranged by size: 2276 1275 330 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1689 2469 2911 3522 3945 Size: 1689 780 442 611 423 Fragments arranged by size: 1689 780 611 442 423 XmaI C'CCGG_G Cuts at: 0 440 3945 Size: 440 3505 Enzymes that do cut: AarI AccI AcuI AflIII AhdI AlfI AloI AlwNI ApoI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI BglI BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrDI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NspI PasI PflMI PmlI PpuMI PstI PvuII SacI SanDI SapI SbfI SexAI SfcI SfoI SmaI SmlI SrfI StuI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AleI ApaI ApaLI AscI AseI AsiSI BcgI BclI BfrBI BglII BmgBI BsaBI BsiEI BsiWI BspHI BsrBI BsrFI BssHII BssSI BstBI BstZ17I BtgZI ClaI DraI DraIII EagI EciI EcoRV FseI FspAI HindIII HpaI KpnI MfeI MluI NaeI NgoMIV NotI NruI NsiI PacI PciI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SspI SwaI Tth111I XbaI XhoI XmnI ZraI