Restriction map for
pFN21AE1311
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccII AclI AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BclI BglI BlnI BlpI
Bpu1102I BsaAI BsaI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HhaI HinP1I
HpaI KasI KpnI Ksp632I KspI
MaeII MfeI MluI MluNI MscI
MslI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI StuI TaqI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AccI 1192 GTAGAC GT^[AC][GT]AC
AccIII 1236 TCCGGA T^CCGGA
AcyI 247 GACGCC G[GA]^CG[CT]C
AvaI 203 CCCGGG C^[CT]CG[GA]G
BanII 945 GGGCTC G[GA]GC[CT]^C
BsaHI 247 GACGCC G[GA]^CG[CT]C
BseAI 1236 TCCGGA T^CCGGA
BseRI 400 GAGGAG GAGGAG(10/8)
BsmAI 1025 GAGAC GTCTC(1/5)
BsoBI 203 CCCGGG C^[CT]CG[GA]G
BspEI 1236 TCCGGA T^CCGGA
BsrBI 1279 GAGCGG CCGCTC(-3/-3)
BsrDI 1045 CATTGC GCAATG(2/0)
Cac8I 867 GCAGGC GC[ACGT]^[ACGT]GC
Cfr13I 783 GGGCC G^G[ACGT]CC
DraI 570 TTTAAA TTT^AAA
EcoT14I 170 CCAAGG C^C[AT][AT]GG
HaeIII 784 GGCC GG^CC
HgaI 247 GACGC GACGC(5/10)
Hin1I 247 GACGCC G[GA]^CG[CT]C
HincII 1119 GTCAAC GT[CT]^[GA]AC
HindII 1119 GTCAAC GT[CT]^[GA]AC
HindIII 661 AAGCTT A^AGCTT
MroI 1236 TCCGGA T^CCGGA
PstI 38 CTGCAG CTGCA^G
Sau96I 783 GGGCC G^G[ACGT]CC
SmaI 203 CCCGGG CCC^GGG
StyI 170 CCAAGG C^C[AT][AT]GG
SwaI 569 ATTTAAAT ATTT^AAAT
TfiI 223 GATTC G^A[AT]TC
XmaCI 203 CCCGGG C^CCGGG
XmaI 203 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AciI 553 GCGG CCGC(-3/-1)
1281 GCGG
AflII 54 CTTAAG C^TTAAG
1051 CTTAAG
AlwNI 19 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
41 CAGATCCTG
AseI 163 ATTAAT AT^TAAT
1243 ATTAAT
AsnI 163 ATTAAT AT^TAAT
1243 ATTAAT
Asp700I 1294 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1310 GAATAATTTC
AspHI 693 GAGCAC G[AT]GC[AT]^C
767 GAGCAC
BbsI 495 GAAGAC GAAGAC(2/6)
1176 GTCTTC
BciVI 756 GGATAC GTATCC(6/5)
613 GTATCC
BcnI 203 CCCGG CC^[GC]GG
204 CCGGG
BfrI 54 CTTAAG C^TTAAG
1051 CTTAAG
BpuAI 495 GAAGAC GAAGAC(2/6)
1176 GTCTTC
BsaBI 308 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
568 GATTTAAATC
BsaJI 170 CCAAGG C^C[ACGT][ACGT]GG
203 CCCGGG
BsaWI 73 TCCGGT [AT]^CCGG[AT]
1236 TCCGGA
BsgI 350 GTGCAG GTGCAG(16/14)
865 GTGCAG
BsiHKAI 693 GAGCAC G[AT]GC[AT]^C
767 GAGCAC
BstNI 379 CCTGG CC^[AT]GG
438 CCAGG
EcoRII 379 CCTGG ^CC[AT]GG
438 CCAGG
MamI 308 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
568 GATTTAAATC
MvaI 379 CCTGG CC^[AT]GG
438 CCAGG
MwoI 297 GCTGGATAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
871 GCTAGAGCTGC
NciI 203 CCCGG CC^[GC]GG
204 CCGGG
NlaIII 532 CATG CATG^
1081 CATG
PshBI 163 ATTAAT AT^TAAT
1243 ATTAAT
SmlI 54 CTTAAG C^T[CT][GA]AG
1051 CTTAAG
SspI 521 AATATT AAT^ATT
1010 AATATT
Tsp45I 59 GTGAC ^GT[GC]AC
245 GTGAC
XmnI 1294 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1310 GAATAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BfaI BglII BmyI BpmI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstYI DdeI DpnII
Fnu4HI FokI HapII HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MspI
NdeII PleI RsaI Sau3AI ScrFI
SfcI Tru9I TseI Tsp509I TspRI
XhoII