(Circular) (Six-base) MAPSORT of: pFN21AE1311.seq Check: 8259 from: 1 to: 6112 pFN21AE1311 6112 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3922 278 Size: 53 83 186 936 2386 2468 Fragments arranged by size: 2468 2386 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3198 3455 1937 Size: 1261 257 4594 Fragments arranged by size: 4594 1261 257 Acc65I G'GTAC_C Cuts at: 3433 3433 Size: 6112 AclI AA'CG_TT Cuts at: 1563 4239 4612 1563 Size: 2676 373 3063 Fragments arranged by size: 3063 2676 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3060 4175 5559 5827 1632 Size: 1428 1115 1384 268 1917 Fragments arranged by size: 1917 1428 1384 1115 268 AflII C'TTAA_G Cuts at: 820 1017 2059 3056 820 Size: 197 1042 997 3876 Fragments arranged by size: 3876 1042 997 197 AflIII A'CryG_T Cuts at: 1203 5011 5988 1203 Size: 3808 977 1327 Fragments arranged by size: 3808 1327 977 AgeI A'CCGG_T Cuts at: 4943 4943 Size: 6112 AhdI GACnn_n'nnGTC Cuts at: 4841 4841 Size: 6112 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6112 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6112 AlwNI CAG_nnn'CTG Cuts at: 2029 2051 3855 5427 2029 Size: 22 1804 1572 2714 Fragments arranged by size: 2714 1804 1572 22 ApaLI G'TGCA_C Cuts at: 4169 5325 4169 Size: 1156 4956 ApoI r'AATT_y Cuts at: 1493 2820 3225 3237 3421 3647 3799 5908 1493 Size: 1327 405 12 184 226 152 2109 1697 Fragments arranged by size: 2109 1697 1327 405 226 184 152 12 AseI AT'TA_AT Cuts at: 160 1032 2169 3249 4666 160 Size: 872 1137 1080 1417 1606 Fragments arranged by size: 1606 1417 1137 1080 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6112 AvaI C'yCGr_G Cuts at: 1870 1943 2208 3437 1870 Size: 73 265 1229 4545 Fragments arranged by size: 4545 1229 265 73 BamHI G'GATC_C Cuts at: 3442 3809 3442 Size: 367 5745 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3433 4888 618 Size: 325 217 627 1646 1455 1842 Fragments arranged by size: 1842 1646 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2954 3431 1391 Size: 1563 477 4072 Fragments arranged by size: 4072 1563 477 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2507 3174 928 Size: 971 608 667 3866 Fragments arranged by size: 3866 971 667 608 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4303 4337 1903 Size: 34 2366 34 3678 Fragments arranged by size: 3678 2366 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2629 2755 4004 5214 2629 Size: 126 1249 1210 3527 Fragments arranged by size: 3527 1249 1210 126 BclI T'GATC_A Cuts at: 1548 1548 Size: 6112 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4723 136 Size: 107 122 71 631 605 3051 1525 Fragments arranged by size: 3051 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2318 2740 3337 6107 2318 Size: 422 597 2770 2323 Fragments arranged by size: 2770 2323 597 422 BlpI GC'TnA_GC Cuts at: 3529 3529 Size: 6112 Bme1580I G_kGCm'C Cuts at: 1790 4173 5329 1790 Size: 2383 1156 2573 Fragments arranged by size: 2573 2383 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6112 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3843 4801 442 Size: 416 53 83 399 24 2426 958 1753 Fragments arranged by size: 2426 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6112 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2976 3048 3150 3302 4772 1239 Size: 1737 72 102 152 1470 2579 Fragments arranged by size: 2579 1737 1470 152 102 72 Bpu10I CC'TnA_GC Cuts at: 2623 2623 Size: 6112 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4237 5102 5400 4237 Size: 865 298 4949 Fragments arranged by size: 4949 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4775 882 Size: 617 3276 2219 Fragments arranged by size: 3276 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3903 493 Size: 711 2699 2702 Fragments arranged by size: 2702 2699 711 BsaBI GATnn'nnATC Cuts at: 2317 2577 6106 2317 Size: 260 3529 2323 Fragments arranged by size: 3529 2323 260 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2253 3919 4301 4936 Size: 53 83 186 936 720 1666 382 635 Cuts at: 4936 275 Size: 1451 Fragments arranged by size: 1666 1451 936 720 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2078 3241 4545 4943 5217 5364 5775 2078 Size: 1163 1304 398 274 147 411 2415 Fragments arranged by size: 2415 1304 1163 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2592 2622 1178 Size: 30 165 30 1189 30 4668 Fragments arranged by size: 4668 1189 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2420 1186 Size: 1234 4878 BseYI C'CCAG_C Cuts at: 2096 5315 5811 2096 Size: 3219 496 2397 Fragments arranged by size: 3219 2397 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2376 2891 1163 Size: 573 640 515 4384 Fragments arranged by size: 4384 640 573 515 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3562 4323 5351 1540 Size: 275 186 1561 761 1028 2301 Fragments arranged by size: 2301 1561 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2702 2776 4173 4258 5329 2702 Size: 74 1397 85 1071 3485 Fragments arranged by size: 3485 1397 1071 85 74 BsmI GAATG_Cn' Cuts at: 3623 3716 3623 Size: 93 6019 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2702 2776 2954 3431 4173 4258 5329 Size: 399 912 74 178 477 742 85 1071 Cuts at: 5329 1391 Size: 2174 Fragments arranged by size: 2174 1071 912 742 477 399 178 85 74 BspEI T'CCGG_A Cuts at: 3241 5775 3241 Size: 2534 3578 BspHI T'CATG_A Cuts at: 4001 5731 4001 Size: 1730 4382 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3467 844 Size: 2623 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3286 3999 1394 Size: 429 1463 713 3507 Fragments arranged by size: 3507 1463 713 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3049 4607 4781 65 Size: 1183 1801 1558 174 1396 Fragments arranged by size: 1801 1558 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4756 4943 1700 Size: 110 2946 187 2869 Fragments arranged by size: 2946 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6112 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6112 BssSI C'ACGA_G Cuts at: 4172 5184 4172 Size: 1012 5100 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6112 BstBI TT'CG_AA Cuts at: 4916 4916 Size: 6112 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6112 BstYI r'GATC_y Cuts at: 1972 2047 2318 2422 2740 3337 3442 3809 4194 Size: 75 271 104 318 597 105 367 385 Cuts at: 4194 4211 5663 5749 5761 6107 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 597 385 367 346 318 271 105 104 86 75 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4621 Fragments arranged by size: 4621 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5373 Fragments arranged by size: 5373 683 56 BtsI GCAGTG_nn' Cuts at: 3628 4422 4442 3628 Size: 794 20 5298 Fragments arranged by size: 5298 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6112 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2691 2726 529 Size: 35 2127 35 3915 Fragments arranged by size: 3915 2127 35 35 DraI TTT'AAA Cuts at: 2577 3416 2577 Size: 839 5273 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5119 809 Size: 507 3803 1802 Fragments arranged by size: 3803 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3559 4448 6077 Size: 54 1425 50 275 18 1729 889 1629 Cuts at: 6077 8 Size: 43 Fragments arranged by size: 1729 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3559 1537 Size: 275 1747 4090 Fragments arranged by size: 4090 1747 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4042 1353 Size: 2689 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4699 5073 5219 387 Size: 1064 138 3110 374 146 1280 Fragments arranged by size: 3110 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2976 3048 3060 3150 3302 4175 4772 Size: 393 1344 72 12 90 152 873 597 Cuts at: 4772 5559 5827 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1344 873 787 597 393 268 152 90 72 12 EcoRI G'AATT_C Cuts at: 3421 3799 3421 Size: 378 5734 FspI TGC'GCA Cuts at: 4618 4618 Size: 6112 HaeII r_GCGC'y Cuts at: 1678 5259 1678 Size: 3581 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2297 2329 2645 2677 4753 Size: 32 135 32 725 32 316 32 2076 Cuts at: 4753 4785 4827 4859 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2076 725 316 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3126 3456 3702 669 Size: 988 281 1188 330 246 3079 Fragments arranged by size: 3079 1188 988 330 281 246 HindIII A'AGCT_T Cuts at: 748 2666 748 Size: 1918 4194 HpaI GTT'AAC Cuts at: 3702 3702 Size: 6112 KpnI G_GTAC'C Cuts at: 3437 3437 Size: 6112 MfeI C'AATT_G Cuts at: 3711 3711 Size: 6112 MluI A'CGCG_T Cuts at: 5988 5988 Size: 6112 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2791 5226 5410 1305 Size: 1486 2435 184 2007 Fragments arranged by size: 2435 2007 1486 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4290 Fragments arranged by size: 4290 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4070 4429 4588 518 Size: 648 2904 359 159 2042 Fragments arranged by size: 2904 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3528 4205 5353 5598 1606 Size: 1922 677 1148 245 2120 Fragments arranged by size: 2120 1922 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4621 Fragments arranged by size: 4621 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6112 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6112 NotI GC'GGCC_GC Cuts at: 3559 3559 Size: 6112 NspI r_CATG'y Cuts at: 1132 1229 3470 5015 1132 Size: 97 2241 1545 2229 Fragments arranged by size: 2241 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6112 PciI A'CATG_T Cuts at: 5011 5011 Size: 6112 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6112 PmeI GTTT'AAAC Cuts at: 3416 3416 Size: 6112 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4181 4213 5694 5726 4181 Size: 32 1481 32 4567 Fragments arranged by size: 4567 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6112 PsiI TTA'TAA Cuts at: 3110 3682 3110 Size: 572 5540 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3054 3086 3054 Size: 32 6080 PstI C_TGCA'G Cuts at: 830 2047 3464 830 Size: 1217 1417 3478 Fragments arranged by size: 3478 1417 1217 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6112 SalI G'TCGA_C Cuts at: 1936 3454 1936 Size: 1518 4594 SbfI CC_TGCA'GG Cuts at: 3464 3464 Size: 6112 ScaI AGT'ACT Cuts at: 4360 6072 4360 Size: 1712 4400 SfcI C'TryA_G Cuts at: 826 1045 2043 2466 2859 3195 3460 4595 5276 Size: 219 998 423 393 336 265 1135 681 Cuts at: 5276 5467 826 Size: 191 1471 Fragments arranged by size: 1471 1135 998 681 423 393 336 265 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6112 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6112 SmaI CCC'GGG Cuts at: 1872 2210 3439 1872 Size: 338 1229 4545 Fragments arranged by size: 4545 1229 338 SmlI C'TyrA_G Cuts at: 820 1017 1943 2059 3056 4216 5117 5379 820 Size: 197 926 116 997 1160 901 262 1553 Fragments arranged by size: 1553 1160 997 926 901 262 197 116 SnaBI TAC'GTA Cuts at: 493 3903 493 Size: 3410 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6112 SphI G_CATG'C Cuts at: 1132 3470 1132 Size: 2338 3774 SspI AAT'ATT Cuts at: 5 52 2528 3017 4036 5 Size: 47 2476 489 1019 2081 Fragments arranged by size: 2476 2081 1019 489 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6112 StyI C'CwwG_G Cuts at: 513 1065 2004 2175 3551 513 Size: 552 939 171 1376 3074 Fragments arranged by size: 3074 1376 939 552 171 SwaI ATTT'AAAT Cuts at: 2577 2577 Size: 6112 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2221 4139 4156 4309 4494 4939 325 Size: 1255 641 1918 17 153 185 445 1498 Fragments arranged by size: 1918 1498 1255 641 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3137 4358 6070 Size: 274 80 33 51 1443 1160 1221 1712 Cuts at: 6070 96 Size: 138 Fragments arranged by size: 1712 1443 1221 1160 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2259 2291 2592 2624 6072 6104 2259 Size: 32 301 32 3448 32 2267 Fragments arranged by size: 3448 2267 301 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6112 XbaI T'CTAG_A Cuts at: 3448 3448 Size: 6112 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6112 XmaI C'CCGG_G Cuts at: 1870 2208 3437 1870 Size: 338 1229 4545 Fragments arranged by size: 4545 1229 338 XmnI GAAnn'nnTTC Cuts at: 3303 3319 4241 3303 Size: 16 922 5174 Fragments arranged by size: 5174 922 16 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3920 276 Size: 53 83 186 936 2386 2468 Fragments arranged by size: 2468 2386 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI XcmI