(Circular) (Six-base) MAPSORT of: pFN21AE3648.seq Check: 7292 from: 1 to: 5407 pFN21AE3648 5407 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2437 2437 Size: 5407 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2063 3217 278 Size: 53 83 186 936 527 1154 2468 Fragments arranged by size: 2468 1154 936 527 186 83 53 AccI GT'mk_AC Cuts at: 1937 2750 1937 Size: 813 4594 Acc65I G'GTAC_C Cuts at: 2728 2728 Size: 5407 AclI AA'CG_TT Cuts at: 1563 3534 3907 1563 Size: 1971 373 3063 Fragments arranged by size: 3063 1971 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2153 3470 4854 5122 1632 Size: 521 1317 1384 268 1917 Fragments arranged by size: 1917 1384 1317 521 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5210 AflIII A'CryG_T Cuts at: 1203 4306 5283 1203 Size: 3103 977 1327 Fragments arranged by size: 3103 1327 977 AgeI A'CCGG_T Cuts at: 2283 4238 2283 Size: 1955 3452 AhdI GACnn_n'nnGTC Cuts at: 4136 4136 Size: 5407 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5407 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2045 1184 Size: 861 4546 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2270 2302 2270 Size: 32 5375 AlwNI CAG_nnn'CTG Cuts at: 2266 3150 4722 2266 Size: 884 1572 2951 Fragments arranged by size: 2951 1572 884 ApaLI G'TGCA_C Cuts at: 3464 4620 3464 Size: 1156 4251 ApoI r'AATT_y Cuts at: 1493 2716 2942 3094 5203 1493 Size: 1223 226 152 2109 1697 Fragments arranged by size: 2109 1697 1223 226 152 AseI AT'TA_AT Cuts at: 160 1032 3961 160 Size: 872 2929 1606 Fragments arranged by size: 2929 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5407 AvaI C'yCGr_G Cuts at: 1870 1943 2732 1870 Size: 73 789 4545 Fragments arranged by size: 4545 789 73 BamHI G'GATC_C Cuts at: 2737 3104 2737 Size: 367 5040 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2120 2437 2608 2728 4183 Size: 325 217 627 333 317 171 120 1455 Cuts at: 4183 618 Size: 1842 Fragments arranged by size: 1842 1455 627 333 325 317 217 171 120 BanII G_rGCy'C Cuts at: 1391 2204 2726 1391 Size: 813 522 4072 Fragments arranged by size: 4072 813 522 BbeI G_GCGC'C Cuts at: 2124 2124 Size: 5407 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2063 928 Size: 971 164 4272 Fragments arranged by size: 4272 971 164 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3598 3632 1903 Size: 34 1661 34 3678 Fragments arranged by size: 3678 1661 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3299 4509 3299 Size: 1210 4197 BclI T'GATC_A Cuts at: 1548 1548 Size: 5407 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4018 136 Size: 107 122 71 631 605 2346 1525 Fragments arranged by size: 2346 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2690 5402 2690 Size: 2712 2695 BlpI GC'TnA_GC Cuts at: 2529 2824 2529 Size: 295 5112 Bme1580I G_kGCm'C Cuts at: 1790 2440 3468 4624 1790 Size: 650 1028 1156 2573 Fragments arranged by size: 2573 1156 1028 650 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5407 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3138 4096 442 Size: 416 53 83 399 24 1721 958 1753 Fragments arranged by size: 1753 1721 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5407 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2297 2477 4067 1239 Size: 1058 180 1590 2579 Fragments arranged by size: 2579 1590 1058 180 Bpu10I CC'TnA_GC Cuts at: 2442 2442 Size: 5407 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2474 3532 4397 4695 2474 Size: 1058 865 298 3186 Fragments arranged by size: 3186 1058 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2540 2582 4070 882 Size: 617 1041 42 1488 2219 Fragments arranged by size: 2219 1488 1041 617 42 BsaAI yAC'GTr Cuts at: 493 1204 3198 493 Size: 711 1994 2702 Fragments arranged by size: 2702 1994 711 BsaBI GATnn'nnATC Cuts at: 2250 5401 2250 Size: 3151 2256 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2060 2121 3214 3596 Size: 53 83 186 936 527 61 1093 382 Cuts at: 3596 4231 275 Size: 635 1451 Fragments arranged by size: 1451 1093 936 635 527 382 186 83 61 53 BsaWI w'CCGG_w Cuts at: 2283 3840 4238 4512 4659 5070 2283 Size: 1557 398 274 147 411 2620 Fragments arranged by size: 2620 1557 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2141 2171 2270 2300 2411 Size: 30 165 30 738 30 99 30 111 Cuts at: 2411 2441 1178 Size: 30 4144 Fragments arranged by size: 4144 738 165 111 99 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2375 1186 Size: 1189 4218 BseYI C'CCAG_C Cuts at: 2414 4610 5106 2414 Size: 2196 496 2715 Fragments arranged by size: 2715 2196 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2416 2471 1163 Size: 573 680 55 4099 Fragments arranged by size: 4099 680 573 55 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2226 2526 2857 3618 4646 1540 Size: 275 186 225 300 331 761 1028 2301 Fragments arranged by size: 2301 1028 761 331 300 275 225 186 BsiHKAI G_wGCw'C Cuts at: 2034 3468 3553 4624 2034 Size: 1434 85 1071 2817 Fragments arranged by size: 2817 1434 1071 85 BsmI GAATG_Cn' Cuts at: 2918 3011 2918 Size: 93 5314 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2204 2440 2726 3468 3553 4624 Size: 399 244 170 236 286 742 85 1071 Cuts at: 4624 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 286 244 236 170 85 BspEI T'CCGG_A Cuts at: 5070 5070 Size: 5407 BspHI T'CATG_A Cuts at: 3296 5026 3296 Size: 1730 3677 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2437 2762 844 Size: 1593 325 3489 Fragments arranged by size: 3489 1593 325 BsrBI CCG'CTC Cuts at: 1394 1823 2086 3294 1394 Size: 429 263 1208 3507 Fragments arranged by size: 3507 1208 429 263 BsrDI GCAATG_nn' Cuts at: 65 1248 3902 4076 65 Size: 1183 2654 174 1396 Fragments arranged by size: 2654 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2256 2283 2521 2561 4051 4238 1700 Size: 110 446 27 238 40 1490 187 2869 Fragments arranged by size: 2869 1490 446 238 187 110 40 27 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5407 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5407 BssSI C'ACGA_G Cuts at: 3467 4479 3467 Size: 1012 4395 BstAPI GCAn_nnn'nTGC Cuts at: 794 2319 2518 794 Size: 1525 199 3683 Fragments arranged by size: 3683 1525 199 BstBI TT'CG_AA Cuts at: 4211 4211 Size: 5407 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5407 BstYI r'GATC_y Cuts at: 1972 2484 2690 2737 3104 3489 3506 4958 5044 Size: 512 206 47 367 385 17 1452 86 Cuts at: 5044 5056 5402 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 512 385 367 346 206 86 47 17 12 BtgI C'CryG_G Cuts at: 513 1065 2191 513 Size: 552 1126 3729 Fragments arranged by size: 3729 1126 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4668 Fragments arranged by size: 4668 683 56 BtsI GCAGTG_nn' Cuts at: 2144 2923 3717 3737 2144 Size: 779 794 20 3814 Fragments arranged by size: 3814 794 779 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5407 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5372 DraI TTT'AAA Cuts at: 2711 2711 Size: 5407 DraIII CAC_nnn'GTG Cuts at: 2653 2653 Size: 5407 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4414 809 Size: 507 3098 1802 Fragments arranged by size: 3098 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2223 2523 2854 Size: 54 1425 50 275 18 393 300 331 Cuts at: 2854 3743 5372 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 393 331 300 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2223 2523 2854 1537 Size: 275 411 300 331 4090 Fragments arranged by size: 4090 411 331 300 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2330 2385 3337 1353 Size: 977 55 952 3423 Fragments arranged by size: 3423 977 952 55 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3994 4368 4514 387 Size: 1064 138 2405 374 146 1280 Fragments arranged by size: 2405 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2153 2297 2477 3470 4067 4854 5122 Size: 393 521 144 180 993 597 787 268 Cuts at: 5122 1239 Size: 1524 Fragments arranged by size: 1524 993 787 597 521 393 268 180 144 EcoNI CCTnn'n_nnAGG Cuts at: 2357 2357 Size: 5407 EcoO109I rG'GnC_Cy Cuts at: 2367 2367 Size: 5407 EcoRI G'AATT_C Cuts at: 2716 3094 2716 Size: 378 5029 FspI TGC'GCA Cuts at: 3913 3913 Size: 5407 HaeII r_GCGC'y Cuts at: 1678 2092 2124 2146 2556 4554 1678 Size: 414 32 22 410 1998 2531 Fragments arranged by size: 2531 1998 414 410 32 22 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2411 2443 2627 2659 4048 Size: 32 135 32 839 32 184 32 1389 Cuts at: 4048 4080 4122 4154 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1389 839 184 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2751 2997 669 Size: 988 281 813 246 3079 Fragments arranged by size: 3079 988 813 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5407 HpaI GTT'AAC Cuts at: 2997 2997 Size: 5407 KasI G'GCGC_C Cuts at: 2120 2120 Size: 5407 KpnI G_GTAC'C Cuts at: 2732 2732 Size: 5407 MfeI C'AATT_G Cuts at: 3006 3006 Size: 5407 MluI A'CGCG_T Cuts at: 5283 5283 Size: 5407 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4521 4705 1305 Size: 3216 184 2007 Fragments arranged by size: 3216 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3585 Fragments arranged by size: 3585 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2048 3365 3724 3883 518 Size: 648 882 1317 359 159 2042 Fragments arranged by size: 2042 1317 882 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2074 2455 2528 2823 3500 4648 4893 1606 Size: 468 381 73 295 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 468 381 295 245 73 NaeI GCC'GGC Cuts at: 2523 2523 Size: 5407 NarI GG'CG_CC Cuts at: 2121 2121 Size: 5407 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4855 NdeI CA'TA_TG Cuts at: 387 387 Size: 5407 NgoMIV G'CCGG_C Cuts at: 2521 2521 Size: 5407 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5407 NotI GC'GGCC_GC Cuts at: 2223 2854 2223 Size: 631 4776 NspI r_CATG'y Cuts at: 1132 1229 2765 4310 1132 Size: 97 1536 1545 2229 Fragments arranged by size: 2229 1545 1536 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5407 PciI A'CATG_T Cuts at: 4306 4306 Size: 5407 PflMI CCAn_nnn'nTGG Cuts at: 1108 2197 1108 Size: 1089 4318 PfoI T'CCnGG_A Cuts at: 2094 2094 Size: 5407 PmeI GTTT'AAAC Cuts at: 2711 2711 Size: 5407 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2270 2302 3476 3508 4989 5021 2270 Size: 32 1174 32 1481 32 2656 Fragments arranged by size: 2656 1481 1174 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5407 PsiI TTA'TAA Cuts at: 2478 2977 2478 Size: 499 4908 PstI C_TGCA'G Cuts at: 830 2073 2759 830 Size: 1243 686 3478 Fragments arranged by size: 3478 1243 686 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5407 PvuII CAG'CTG Cuts at: 2455 2455 Size: 5407 SalI G'TCGA_C Cuts at: 1936 2749 1936 Size: 813 4594 SbfI CC_TGCA'GG Cuts at: 2759 2759 Size: 5407 ScaI AGT'ACT Cuts at: 3655 5367 3655 Size: 1712 3695 SexAI A'CCwGG_T Cuts at: 2601 2601 Size: 5407 SfcI C'TryA_G Cuts at: 826 1045 2069 2755 3890 4571 4762 826 Size: 219 1024 686 1135 681 191 1471 Fragments arranged by size: 1471 1135 1024 686 681 219 191 SfoI GGC'GCC Cuts at: 2122 2122 Size: 5407 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5407 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5407 SmaI CCC'GGG Cuts at: 1872 2734 1872 Size: 862 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2489 3511 4412 4674 820 Size: 197 926 546 1022 901 262 1553 Fragments arranged by size: 1553 1022 926 901 546 262 197 SnaBI TAC'GTA Cuts at: 493 3198 493 Size: 2705 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5407 SphI G_CATG'C Cuts at: 1132 2765 1132 Size: 1633 3774 SspI AAT'ATT Cuts at: 5 52 3331 5 Size: 47 3279 2081 Fragments arranged by size: 3279 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5407 StyI C'CwwG_G Cuts at: 513 1065 2370 2432 2846 513 Size: 552 1305 62 414 3074 Fragments arranged by size: 3074 1305 552 414 62 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2685 2692 3434 3451 3604 3789 4234 Size: 1255 1105 7 742 17 153 185 445 Cuts at: 4234 325 Size: 1498 Fragments arranged by size: 1498 1255 1105 742 445 185 153 17 7 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2208 2493 3653 Size: 274 80 33 51 1443 231 285 1160 Cuts at: 3653 5365 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1160 285 274 231 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5367 5399 5367 Size: 32 5375 Tth111I GACn'n_nGTC Cuts at: 1316 2102 2423 1316 Size: 786 321 4300 Fragments arranged by size: 4300 786 321 XbaI T'CTAG_A Cuts at: 2743 2743 Size: 5407 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5407 XmaI C'CCGG_G Cuts at: 1870 2732 1870 Size: 862 4545 XmnI GAAnn'nnTTC Cuts at: 3536 3536 Size: 5407 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2061 3215 276 Size: 53 83 186 936 527 1154 2468 Fragments arranged by size: 2468 1154 936 527 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI XcmI