################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII BamHI BbrPI BciVI BclI BfrI BlpI Bpu1102I BsaBI BsaI BseAI BsgI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI MamI MfeI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsaI RsrII SalI SapI SbfI ScaI SexAI SfcI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Asp700I 1014 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 44 GAGCTC G[AT]GC[AT]^C AvaII 642 GGTCC G^G[AT]CC AvrII 55 CCTAGG C^CTAGG BbeI 903 GGCGCC GGCGC^C BbsI 682 GAAGAC GAAGAC(2/6) BbvCI 459 CCTCAGC CCTCAGC(-5/-2) BfaI 56 CTAG C^TAG BglII 1166 AGATCT A^GATCT BlnI 55 CCTAGG C^CTAGG BpuAI 682 GAAGAC GAAGAC(2/6) BsaAI 1114 TACGTG [CT]AC^GT[GA] BsiEI 1067 CGACCG CG[GA][CT]^CG BsiHKAI 44 GAGCTC G[AT]GC[AT]^C BsmAI 106 GAGAC GTCTC(1/5) BspHI 344 TCATGA T^CATGA BsrBI 1135 GAGCGG CCGCTC(-3/-3) BsrFI 1128 GCCGGC [GA]^CCGG[CT] BstAPI 487 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 296 CCATCGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 1128 GCCGGC [GA]^CCGG[CT] EarI 193 CTCTTC CTCTTC(1/4) HaeII 903 GGCGCC [GA]GCGC^[CT] HincII 352 GTCAAC GT[CT]^[GA]AC HindII 352 GTCAAC GT[CT]^[GA]AC KasI 903 GGCGCC G^GCGCC Ksp632I 193 CTCTTC CTCTTC(1/4) KspI 318 CCGCGG CCGC^GG MaeI 56 CTAG C^TAG NaeI 1128 GCCGGC GCC^GGC NarI 903 GGCGCC GG^CGCC NgoMIV 1128 GCCGGC G^CCGGC NspI 130 ACATGC [GA]CATG^[CT] RcaI 344 TCATGA T^CATGA SacI 44 GAGCTC GAGCT^C SacII 318 CCGCGG CCGC^GG SfiI 1041 GGCCAAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 903 GGCGCC GGC^GCC TfiI 885 GAATC G^A[AT]TC Tsp509I 444 AATT ^AATT XmnI 1014 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcyI 903 GGCGCC G[GA]^CG[CT]C 1150 GACGCC AlwI 1095 GATCC GGATC(4/5) 554 GGATC ApaI 30 GGGCCC GGGCC^C 373 GGGCCC BalI 239 TGGCCA TGG^CCA 1040 TGGCCA BanI 903 GGCGCC G^G[CT][GA]CC 1177 GGCACC BcgI 815 GCATCGGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 890 GCATGGCCTTCG BsaHI 903 GGCGCC G[GA]^CG[CT]C 1150 GACGCC BsaWI 334 ACCGGA [AT]^CCGG[AT] 1229 ACCGGA BseRI 256 GAGGAG GAGGAG(10/8) 307 GAGGAG BsmFI 137 GGGAC GGGAC(10/14) 643 GTCCC BsrDI 165 CATTGC GCAATG(2/0) 843 CATTGC BstYI 1094 AGATCC [GA]^GATC[CT] 1166 AGATCT HgaI 1150 GACGC GACGC(5/10) 496 GCGTC Hin1I 903 GGCGCC G[GA]^CG[CT]C 1150 GACGCC MflI 1094 AGATCC [GA]^GATC[CT] 1166 AGATCT MluNI 239 TGGCCA TGG^CCA 1040 TGGCCA MscI 239 TGGCCA TGG^CCA 1040 TGGCCA MslI 243 CATGGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 297 CATCGCCATG PleI 523 GACTC GAGTC(4/5) 338 GAGTC SmaI 20 CCCGGG CCC^GGG 509 CCCGGG TaqI 278 TCGA T^CGA 860 TCGA TthHB8I 278 TCGA T^CGA 860 TCGA XhoII 1094 AGATCC [GA]^GATC[CT] 1166 AGATCT XmaCI 20 CCCGGG C^CCGGG 509 CCCGGG XmaI 20 CCCGGG C^CCGGG 509 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AccII AciI AluI AlwNI AvaI BanII BbvI BcnI BglI BmyI BpmI BsaJI BsiYI BslI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI Sau3AI Sau96I ScrFI SfaNI StyI TseI Tsp45I TspRI Van91I XcmI