(Circular) (Six-base) MAPSORT of: pFN21AE4390.seq Check: 1658 from: 1 to: 5941 pFN21AE4390 5941 bp With 182 enzymes: SgfI * March 28, 2012 14:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3751 278 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3284 1937 Size: 1347 4594 Acc65I G'GTAC_C Cuts at: 3262 3262 Size: 5941 AclI AA'CG_TT Cuts at: 1563 4068 4441 1563 Size: 2505 373 3063 Fragments arranged by size: 3063 2505 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3026 4004 5388 5656 1632 Size: 1394 978 1384 268 1917 Fragments arranged by size: 1917 1394 1384 978 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5744 AflIII A'CryG_T Cuts at: 1203 4840 5817 1203 Size: 3637 977 1327 Fragments arranged by size: 3637 1327 977 AgeI A'CCGG_T Cuts at: 4772 4772 Size: 5941 AhdI GACnn_n'nnGTC Cuts at: 4670 4670 Size: 5941 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5941 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5941 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2184 2216 2184 Size: 32 5909 AlwNI CAG_nnn'CTG Cuts at: 2498 2842 3103 3202 3684 5256 2498 Size: 344 261 99 482 1572 3183 Fragments arranged by size: 3183 1572 482 344 261 99 ApaI G_GGCC'C Cuts at: 2039 2382 2039 Size: 343 5598 ApaLI G'TGCA_C Cuts at: 3998 5154 3998 Size: 1156 4785 ApoI r'AATT_y Cuts at: 1493 3250 3476 3628 5737 1493 Size: 1757 226 152 2109 1697 Fragments arranged by size: 2109 1757 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4495 160 Size: 872 3463 1606 Fragments arranged by size: 3463 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 AvaI C'yCGr_G Cuts at: 1870 1943 2025 2032 2108 2258 2514 3266 1870 Size: 73 82 7 76 150 256 752 4545 Fragments arranged by size: 4545 752 256 150 82 76 73 7 AvrII C'CTAG_G Cuts at: 2060 2060 Size: 5941 BamHI G'GATC_C Cuts at: 3271 3638 3271 Size: 367 5574 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2908 3182 3262 4717 618 Size: 325 217 627 1121 274 80 1455 1842 Fragments arranged by size: 1842 1455 1121 627 325 274 217 80 BanII G_rGCy'C Cuts at: 1391 2039 2053 2382 2617 2670 2711 3260 1391 Size: 648 14 329 235 53 41 549 4072 Fragments arranged by size: 4072 648 549 329 235 53 41 14 BbeI G_GCGC'C Cuts at: 2912 2912 Size: 5941 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2694 928 Size: 971 795 4175 Fragments arranged by size: 4175 971 795 BbvCI CC'TCA_GC Cuts at: 2465 2465 Size: 5941 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2809 2843 2884 2918 4132 4166 1903 Size: 34 872 34 41 34 1214 34 3678 Fragments arranged by size: 3678 1214 872 41 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3833 5043 3833 Size: 1210 4731 BclI T'GATC_A Cuts at: 1548 1548 Size: 5941 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2576 2823 2837 Size: 107 122 71 631 605 904 247 14 Cuts at: 2837 2906 3053 3065 4552 136 Size: 69 147 12 1487 1525 Fragments arranged by size: 1525 1487 904 631 605 247 147 122 107 71 69 14 12 BglII A'GATC_T Cuts at: 3171 5936 3171 Size: 2765 3176 BlpI GC'TnA_GC Cuts at: 3358 3358 Size: 5941 Bme1580I G_kGCm'C Cuts at: 1790 2039 2259 2382 2977 3185 4002 5158 1790 Size: 249 220 123 595 208 817 1156 2573 Fragments arranged by size: 2573 1156 817 595 249 220 208 123 BmgBI CAC'GTC Cuts at: 1318 2254 1318 Size: 936 5005 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3672 4630 442 Size: 416 53 83 399 24 2255 958 1753 Fragments arranged by size: 2255 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5941 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2545 2545 Size: 5941 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2114 2909 2987 4601 1239 Size: 875 795 78 1614 2579 Fragments arranged by size: 2579 1614 875 795 78 Bpu10I CC'TnA_GC Cuts at: 2211 2465 3224 2211 Size: 254 759 4928 Fragments arranged by size: 4928 759 254 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4066 4931 5229 4066 Size: 865 298 4778 Fragments arranged by size: 4778 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4604 882 Size: 617 3105 2219 Fragments arranged by size: 3105 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3121 3732 493 Size: 711 1917 611 2702 Fragments arranged by size: 2702 1917 711 611 BsaBI GATnn'nnATC Cuts at: 5935 5935 Size: 5941 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2909 3156 3748 4130 Size: 53 83 186 936 1376 247 592 382 Cuts at: 4130 4765 275 Size: 635 1451 Fragments arranged by size: 1451 1376 936 635 592 382 247 186 83 53 BsaWI w'CCGG_w Cuts at: 2339 3234 4374 4772 5046 5193 5604 2339 Size: 895 1140 398 274 147 411 2676 Fragments arranged by size: 2676 1140 895 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5716 Fragments arranged by size: 5716 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2276 2327 1186 Size: 1090 51 4800 Fragments arranged by size: 4800 1090 51 BseYI C'CCAG_C Cuts at: 2203 2695 5144 5640 2203 Size: 492 2449 496 2504 Fragments arranged by size: 2504 2449 496 492 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5368 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3075 3391 4152 5180 1540 Size: 275 186 1074 316 761 1028 2301 Fragments arranged by size: 2301 1074 1028 761 316 275 186 BsiHKAI G_wGCw'C Cuts at: 2053 4002 4087 5158 2053 Size: 1949 85 1071 2836 Fragments arranged by size: 2836 1949 1071 85 BsmI GAATG_Cn' Cuts at: 3452 3545 3452 Size: 93 5848 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2039 2053 2259 2382 2617 2670 2711 Size: 399 249 14 206 123 235 53 41 Cuts at: 2711 2977 3185 3260 4002 4087 5158 1391 Size: 266 208 75 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 266 249 235 208 206 123 85 75 53 41 14 BspEI T'CCGG_A Cuts at: 5604 5604 Size: 5941 BspHI T'CATG_A Cuts at: 2349 3830 5560 2349 Size: 1481 1730 2730 Fragments arranged by size: 2730 1730 1481 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3296 844 Size: 2452 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3142 3828 1394 Size: 429 1319 686 3507 Fragments arranged by size: 3507 1319 686 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2169 2847 4436 4610 65 Size: 1183 921 678 1589 174 1396 Fragments arranged by size: 1589 1396 1183 921 678 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3133 4585 4772 1700 Size: 110 1323 1452 187 2869 Fragments arranged by size: 2869 1452 1323 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5941 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5941 BssSI C'ACGA_G Cuts at: 4001 5013 4001 Size: 1012 4929 BstAPI GCAn_nnn'nTGC Cuts at: 794 2498 794 Size: 1704 4237 BstBI TT'CG_AA Cuts at: 4745 4745 Size: 5941 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5941 BstXI CCAn_nnnn'nTGG Cuts at: 2308 2308 Size: 5941 BstYI r'GATC_y Cuts at: 1972 3099 3171 3271 3638 4023 4040 5492 5578 Size: 1127 72 100 367 385 17 1452 86 Cuts at: 5578 5590 5936 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1127 385 367 346 100 86 72 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2247 2307 2323 2589 2715 3042 Size: 552 939 243 60 16 266 126 327 Cuts at: 3042 513 Size: 3412 Fragments arranged by size: 3412 939 552 327 266 243 126 60 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2287 2812 487 Size: 683 56 1061 525 3616 Fragments arranged by size: 3616 1061 683 525 56 BtsI GCAGTG_nn' Cuts at: 2491 3457 4251 4271 2491 Size: 966 794 20 4161 Fragments arranged by size: 4161 966 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5941 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5906 DraI TTT'AAA Cuts at: 3245 3245 Size: 5941 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4948 809 Size: 507 3632 1802 Fragments arranged by size: 3632 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2244 2718 Size: 54 1425 50 275 18 177 237 474 Cuts at: 2718 3045 3054 3388 4277 5906 8 Size: 327 9 334 889 1629 43 Fragments arranged by size: 1629 1425 889 474 334 327 275 237 177 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 3388 1537 Size: 275 1576 4090 Fragments arranged by size: 4090 1576 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2204 3871 1353 Size: 851 1667 3423 Fragments arranged by size: 3423 1667 851 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4528 4902 5048 387 Size: 1064 138 2939 374 146 1280 Fragments arranged by size: 2939 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2051 2051 Size: 5941 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 2909 2987 3026 4004 4601 5388 Size: 393 482 795 78 39 978 597 787 Cuts at: 5388 5656 1239 Size: 268 1524 Fragments arranged by size: 1524 978 795 787 597 482 393 268 78 39 EcoO109I rG'GnC_Cy Cuts at: 2036 2064 2378 2839 2036 Size: 28 314 461 5138 Fragments arranged by size: 5138 461 314 28 EcoRI G'AATT_C Cuts at: 3250 3628 3250 Size: 378 5563 FspI TGC'GCA Cuts at: 4447 4447 Size: 5941 HaeII r_GCGC'y Cuts at: 1678 2912 5088 1678 Size: 1234 2176 2531 Fragments arranged by size: 2531 2176 1234 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4582 4614 4656 4688 1373 Size: 32 135 32 3010 32 42 32 2626 Fragments arranged by size: 3010 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2359 3285 3531 669 Size: 988 281 421 926 246 3079 Fragments arranged by size: 3079 988 926 421 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5941 HpaI GTT'AAC Cuts at: 3531 3531 Size: 5941 KasI G'GCGC_C Cuts at: 2908 2908 Size: 5941 KpnI G_GTAC'C Cuts at: 3266 3266 Size: 5941 MfeI C'AATT_G Cuts at: 3540 3540 Size: 5941 MluI A'CGCG_T Cuts at: 5817 5817 Size: 5941 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5055 5239 1305 Size: 3750 184 2007 Fragments arranged by size: 3750 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2246 3047 10 Size: 54 1425 343 414 801 2904 Fragments arranged by size: 2904 1425 801 414 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2252 2306 3899 4258 4417 518 Size: 648 1086 54 1593 359 159 2042 Fragments arranged by size: 2042 1593 1086 648 359 159 54 MspA1I CmG'CkG Cuts at: 1606 2207 2325 3199 3357 4034 5182 5427 1606 Size: 601 118 874 158 677 1148 245 2120 Fragments arranged by size: 2120 1148 874 677 601 245 158 118 NaeI GCC'GGC Cuts at: 3135 3135 Size: 5941 NarI GG'CG_CC Cuts at: 2909 2909 Size: 5941 NcoI C'CATG_G Cuts at: 513 1065 2004 2247 2307 3042 513 Size: 552 939 243 60 735 3412 Fragments arranged by size: 3412 939 735 552 243 60 NdeI CA'TA_TG Cuts at: 387 387 Size: 5941 NgoMIV G'CCGG_C Cuts at: 3133 3133 Size: 5941 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5941 NotI GC'GGCC_GC Cuts at: 3388 3388 Size: 5941 NspI r_CATG'y Cuts at: 1132 1229 2139 3299 4844 1132 Size: 97 910 1160 1545 2229 Fragments arranged by size: 2229 1545 1160 910 97 PasI CC'CwG_GG Cuts at: 1348 2842 1348 Size: 1494 4447 PciI A'CATG_T Cuts at: 4840 4840 Size: 5941 PflMI CCAn_nnn'nTGG Cuts at: 1108 2098 2556 2571 2663 2842 3103 3202 1108 Size: 990 458 15 92 179 261 99 3847 Fragments arranged by size: 3847 990 458 261 179 99 92 15 PmeI GTTT'AAAC Cuts at: 3245 3245 Size: 5941 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4010 4042 5523 5555 4010 Size: 32 1481 32 4396 Fragments arranged by size: 4396 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5941 PsiI TTA'TAA Cuts at: 3511 3511 Size: 5941 PspOMI G'GGCC_C Cuts at: 2035 2378 2035 Size: 343 5598 PstI C_TGCA'G Cuts at: 830 3293 830 Size: 2463 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5941 SacI G_AGCT'C Cuts at: 2053 2053 Size: 5941 SacII CC_GC'GG Cuts at: 2326 2326 Size: 5941 SalI G'TCGA_C Cuts at: 1936 3283 1936 Size: 1347 4594 SbfI CC_TGCA'GG Cuts at: 3293 3293 Size: 5941 ScaI AGT'ACT Cuts at: 4189 5901 4189 Size: 1712 4229 SfcI C'TryA_G Cuts at: 826 1045 3289 4424 5105 5296 826 Size: 219 2244 1135 681 191 1471 Fragments arranged by size: 2244 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3053 3053 Size: 5941 SfoI GGC'GCC Cuts at: 2910 2910 Size: 5941 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5941 SmaI CCC'GGG Cuts at: 1872 2027 2516 3268 1872 Size: 155 489 752 4545 Fragments arranged by size: 4545 752 489 155 SmlI C'TyrA_G Cuts at: 820 1017 1943 4045 4946 5208 820 Size: 197 926 2102 901 262 1553 Fragments arranged by size: 2102 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3732 493 Size: 3239 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5941 SphI G_CATG'C Cuts at: 1132 3299 1132 Size: 2167 3774 SrfI GCCC'GGGC Cuts at: 2027 2516 2027 Size: 489 5452 SspI AAT'ATT Cuts at: 5 52 3865 5 Size: 47 3813 2081 Fragments arranged by size: 3813 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5941 StyI C'CwwG_G Cuts at: 513 1065 2004 2060 2247 2307 2400 2751 3042 Size: 552 939 56 187 60 93 351 291 Cuts at: 3042 3380 513 Size: 338 3074 Fragments arranged by size: 3074 939 552 351 338 291 187 93 60 56 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3968 3985 4138 4323 4768 325 Size: 1255 2388 17 153 185 445 1498 Fragments arranged by size: 2388 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4187 5899 96 Size: 274 80 33 51 1443 2210 1712 138 Fragments arranged by size: 2210 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2470 2502 5901 5933 2470 Size: 32 3399 32 2478 Fragments arranged by size: 3399 2478 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5941 XbaI T'CTAG_A Cuts at: 3277 3277 Size: 5941 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2803 3049 3100 2803 Size: 246 51 5644 Fragments arranged by size: 5644 246 51 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5941 XmaI C'CCGG_G Cuts at: 1870 2025 2514 3266 1870 Size: 155 489 752 4545 Fragments arranged by size: 4545 752 489 155 XmnI GAAnn'nnTTC Cuts at: 3023 4070 3023 Size: 1047 4894 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3749 276 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SanDI SapI SexAI SwaI