(Linear) (Six-base) MAPSORT of: KIEE4390.seq Check: 2074 from: 1 to: 1236 KIEE4390.orf With 182 enzymes: SgfI * March 22, 2012 17:54 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1021 1236 Size: 1021 215 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 179 211 1236 Size: 179 32 1025 Fragments arranged by size: 1025 179 32 AlwNI CAG_nnn'CTG Cuts at: 0 493 837 1098 1197 1236 Size: 493 344 261 99 39 Fragments arranged by size: 493 344 261 99 39 ApaI G_GGCC'C Cuts at: 0 34 377 1236 Size: 34 343 859 Fragments arranged by size: 859 343 34 AvaI C'yCGr_G Cuts at: 0 20 27 103 253 509 1236 Size: 20 7 76 150 256 727 Fragments arranged by size: 727 256 150 76 20 7 AvrII C'CTAG_G Cuts at: 0 55 1236 Size: 55 1181 BanI G'GyrC_C Cuts at: 0 903 1177 1236 Size: 903 274 59 Fragments arranged by size: 903 274 59 BanII G_rGCy'C Cuts at: 0 34 48 377 612 665 706 1236 Size: 34 14 329 235 53 41 530 Fragments arranged by size: 530 329 235 53 41 34 14 BbeI G_GCGC'C Cuts at: 0 907 1236 Size: 907 329 BbsI GAAGACnn'nnnn_ Cuts at: 0 689 1236 Size: 689 547 BbvCI CC'TCA_GC Cuts at: 0 460 1236 Size: 460 776 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 804 838 879 913 1236 Size: 804 34 41 34 323 Fragments arranged by size: 804 323 41 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 571 818 832 901 1048 1060 1236 Size: 571 247 14 69 147 12 176 Fragments arranged by size: 571 247 176 147 69 14 12 BglII A'GATC_T Cuts at: 0 1166 1236 Size: 1166 70 Bme1580I G_kGCm'C Cuts at: 0 34 254 377 972 1180 1236 Size: 34 220 123 595 208 56 Fragments arranged by size: 595 220 208 123 56 34 BmgBI CAC'GTC Cuts at: 0 249 1236 Size: 249 987 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 540 1236 Size: 540 696 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 109 904 982 1236 Size: 109 795 78 254 Fragments arranged by size: 795 254 109 78 Bpu10I CC'TnA_GC Cuts at: 0 206 460 1219 1236 Size: 206 254 759 17 Fragments arranged by size: 759 254 206 17 BsaAI yAC'GTr Cuts at: 0 1116 1236 Size: 1116 120 BsaHI Gr'CG_yC Cuts at: 0 904 1151 1236 Size: 904 247 85 Fragments arranged by size: 904 247 85 BsaWI w'CCGG_w Cuts at: 0 334 1229 1236 Size: 334 895 7 Fragments arranged by size: 895 334 7 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 271 322 1236 Size: 271 51 914 Fragments arranged by size: 914 271 51 BseYI C'CCAG_C Cuts at: 0 198 690 1236 Size: 198 492 546 Fragments arranged by size: 546 492 198 BsiEI CG_ry'CG Cuts at: 0 1070 1236 Size: 1070 166 BsiHKAI G_wGCw'C Cuts at: 0 48 1236 Size: 48 1188 Bsp1286I G_dGCh'C Cuts at: 0 34 48 254 377 612 665 706 972 Size: 34 14 206 123 235 53 41 266 Cuts at: 972 1180 1236 Size: 208 56 Fragments arranged by size: 266 235 208 206 123 56 53 41 34 14 BspHI T'CATG_A Cuts at: 0 344 1236 Size: 344 892 BsrBI CCG'CTC Cuts at: 0 1137 1236 Size: 1137 99 BsrDI GCAATG_nn' Cuts at: 0 164 842 1236 Size: 164 678 394 Fragments arranged by size: 678 394 164 BsrFI r'CCGG_y Cuts at: 0 1128 1236 Size: 1128 108 BstAPI GCAn_nnn'nTGC Cuts at: 0 493 1236 Size: 493 743 BstXI CCAn_nnnn'nTGG Cuts at: 0 303 1236 Size: 303 933 BstYI r'GATC_y Cuts at: 0 1094 1166 1236 Size: 1094 72 70 Fragments arranged by size: 1094 72 70 BtgI C'CryG_G Cuts at: 0 242 302 318 584 710 1037 1236 Size: 242 60 16 266 126 327 199 Fragments arranged by size: 327 266 242 199 126 60 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 282 807 1236 Size: 282 525 429 Fragments arranged by size: 525 429 282 BtsI GCAGTG_nn' Cuts at: 0 486 1236 Size: 486 750 EaeI y'GGCC_r Cuts at: 0 2 239 713 1040 1049 1236 Size: 2 237 474 327 9 187 Fragments arranged by size: 474 327 237 187 9 2 EarI CTCTTCn'nnn_ Cuts at: 0 199 1236 Size: 199 1037 EcoICRI GAG'CTC Cuts at: 0 46 1236 Size: 46 1190 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 109 904 982 1021 1236 Size: 109 795 78 39 215 Fragments arranged by size: 795 215 109 78 39 EcoO109I rG'GnC_Cy Cuts at: 0 31 59 373 834 1236 Size: 31 28 314 461 402 Fragments arranged by size: 461 402 314 31 28 HaeII r_GCGC'y Cuts at: 0 907 1236 Size: 907 329 HincII GTy'rAC Cuts at: 0 354 1236 Size: 354 882 KasI G'GCGC_C Cuts at: 0 903 1236 Size: 903 333 MscI TGG'CCA Cuts at: 0 241 1042 1236 Size: 241 801 194 Fragments arranged by size: 801 241 194 MslI CAynn'nnrTG Cuts at: 0 247 301 1236 Size: 247 54 935 Fragments arranged by size: 935 247 54 MspA1I CmG'CkG Cuts at: 0 202 320 1194 1236 Size: 202 118 874 42 Fragments arranged by size: 874 202 118 42 NaeI GCC'GGC Cuts at: 0 1130 1236 Size: 1130 106 NarI GG'CG_CC Cuts at: 0 904 1236 Size: 904 332 NcoI C'CATG_G Cuts at: 0 242 302 1037 1236 Size: 242 60 735 199 Fragments arranged by size: 735 242 199 60 NgoMIV G'CCGG_C Cuts at: 0 1128 1236 Size: 1128 108 NspI r_CATG'y Cuts at: 0 134 1236 Size: 134 1102 PasI CC'CwG_GG Cuts at: 0 837 1236 Size: 837 399 PflMI CCAn_nnn'nTGG Cuts at: 0 93 551 566 658 837 1098 1197 1236 Size: 93 458 15 92 179 261 99 39 Fragments arranged by size: 458 261 179 99 93 92 39 15 PspOMI G'GGCC_C Cuts at: 0 30 373 1236 Size: 30 343 863 Fragments arranged by size: 863 343 30 SacI G_AGCT'C Cuts at: 0 48 1236 Size: 48 1188 SacII CC_GC'GG Cuts at: 0 321 1236 Size: 321 915 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1048 1236 Size: 1048 188 SfoI GGC'GCC Cuts at: 0 905 1236 Size: 905 331 SmaI CCC'GGG Cuts at: 0 22 511 1236 Size: 22 489 725 Fragments arranged by size: 725 489 22 SrfI GCCC'GGGC Cuts at: 0 22 511 1236 Size: 22 489 725 Fragments arranged by size: 725 489 22 StyI C'CwwG_G Cuts at: 0 55 242 302 395 746 1037 1236 Size: 55 187 60 93 351 291 199 Fragments arranged by size: 351 291 199 187 93 60 55 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 465 497 1236 Size: 465 32 739 Fragments arranged by size: 739 465 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 798 1044 1095 1236 Size: 798 246 51 141 Fragments arranged by size: 798 246 141 51 XmaI C'CCGG_G Cuts at: 0 20 509 1236 Size: 20 489 727 Fragments arranged by size: 727 489 20 XmnI GAAnn'nnTTC Cuts at: 0 1018 1236 Size: 1018 218 Enzymes that do cut: AcuI AloI AlwNI ApaI AvaI AvrII BanI BanII BbeI BbsI BbvCI BcgI BglI BglII Bme1580I BmgBI BplI BpmI Bpu10I BsaAI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BsrBI BsrDI BsrFI BstAPI BstXI BstYI BtgI BtgZI BtsI EaeI EarI EcoICRI Eco57MI EcoO109I HaeII HincII KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PspOMI SacI SacII SfiI SfoI SmaI SrfI StyI TstI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI ApaLI ApoI AscI AseI AsiSI BaeI BamHI BciVI BclI BfrBI BlpI BmrI BmtI BpuEI BsaI BsaBI BsaXI BsgI BsiWI BsmI BsmBI BspEI BspMI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI SwaI TaqII TatI Tth111I XbaI XhoI ZraI