################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfaI BglI BlnI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BsrI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I CfoI Cfr10I ClaI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoT22I FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MamI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tth111I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AfaI 834 GTAC GT^AC AflII 1194 CTTAAG C^TTAAG AflIII 314 ACGTGT A^C[GA][CT]GT AocI 1306 CCTGAGG CC^T[ACGT]AGG AspHI 1444 GAGCAC G[AT]GC[AT]^C BanI 61 GGCACC G^G[CT][GA]CC BfrI 1194 CTTAAG C^TTAAG BlpI 903 GCTCAGC GC^T[ACGT]AGC BmyI 1444 GAGCAC G[AGT]GC[ACT]^C Bpu1102I 903 GCTCAGC GC^T[ACGT]AGC BsaI 940 GAGACC GGTCTC(1/5) BsiHKAI 1444 GAGCAC G[AT]GC[AT]^C BsmBI 917 GAGACG CGTCTC(1/5) Bsp1286I 1444 GAGCAC G[AGT]GC[ACT]^C BspHI 1346 TCATGA T^CATGA BspMI 1448 ACCTGC ACCTGC(4/8) BsrDI 38 GCAATG GCAATG(2/0) BstAPI 80 GCATCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 806 CCACACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1306 CCTGAGG CC^T[ACGT]AGG CelII 903 GCTCAGC GC^T[ACGT]AGC CpoI 460 CGGACCG CG^G[AT]CCG Eco81I 1306 CCTGAGG CC^T[ACGT]AGG FauI 824 CCCGC CCCGC(4/6) MseI 1195 TTAA T^TAA NspI 827 GCATGC [GA]CATG^[CT] PshAI 1155 GACTACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1346 TCATGA T^CATGA RsaI 834 GTAC GT^AC RsrII 460 CGGACCG CG^G[AT]CCG SapI 1188 GCTCTTC GCTCTTC(1/4) SphI 827 GCATGC GCATG^C StuI 1166 AGGCCT AGG^CCT Tru9I 1195 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcsI 555 AAATTT [GA]^AATT[CT] 1090 AAATTC ApoI 555 AAATTT [GA]^AATT[CT] 1090 AAATTC AvaI 1324 CTCGAG C^[CT]CG[GA]G 1400 CTCGAG BalI 207 TGGCCA TGG^CCA 869 TGGCCA BbsI 325 GTCTTC GAAGAC(2/6) 856 GTCTTC BclI 378 TGATCA T^GATCA 1286 TGATCA BglII 596 AGATCT A^GATCT 1040 AGATCT BpuAI 325 GTCTTC GAAGAC(2/6) 856 GTCTTC BsoBI 1324 CTCGAG C^[CT]CG[GA]G 1400 CTCGAG BssSI 70 CACGAG CACGAG(-5/-1) 124 CACGAG BstYI 596 AGATCT [GA]^GATC[CT] 1040 AGATCT DraII 334 GGGACCC [GA]G^G[ACGT]CC[CT] 779 AGGACCT EcoO109I 334 GGGACCC [GA]G^G[ACGT]CC[CT] 779 AGGACCT EcoRV 175 GATATC GAT^ATC 1147 GATATC FbaI 378 TGATCA T^GATCA 1286 TGATCA MaeII 314 ACGT A^CGT 614 ACGT MflI 596 AGATCT [GA]^GATC[CT] 1040 AGATCT MluNI 207 TGGCCA TGG^CCA 869 TGGCCA MscI 207 TGGCCA TGG^CCA 869 TGGCCA MslI 681 CATTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1290 CACCGCTGTG PaeR7I 1324 CTCGAG C^TCGAG 1400 CTCGAG PflMI 356 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 861 CCACTCGCTGG PpuMI 334 GGGACCC [GA]G^G[AT]CC[CT] 779 AGGACCT PstI 1276 CTGCAG CTGCA^G 1420 CTGCAG PvuII 433 CAGCTG CAG^CTG 544 CAGCTG Van91I 356 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 861 CCACTCGCTGG XcmI 210 CCAAGTCAAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 260 CCAGCAGCCAGGTGG XhoI 1324 CTCGAG C^TCGAG 1400 CTCGAG XhoII 596 AGATCT [GA]^GATC[CT] 1040 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AluI AlwI AvaII BbvI BpmI BsaJI BsiYI BslI BsmAI BsmFI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeIII MboI MboII MnlI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I