(Circular) (Six-base) MAPSORT of: pFN21AE4472.seq Check: 9557 from: 1 to: 6184 pFN21AE4472 6184 bp With 182 enzymes: SgfI * March 28, 2012 13:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3462 3462 Size: 6184 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3994 278 Size: 53 83 186 936 2458 2468 Fragments arranged by size: 2468 2458 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3527 1937 Size: 1590 4594 Acc65I G'GTAC_C Cuts at: 3505 3505 Size: 6184 AclI AA'CG_TT Cuts at: 1563 4311 4684 1563 Size: 2748 373 3063 Fragments arranged by size: 3063 2748 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2366 2600 2612 3066 4247 5631 5899 1632 Size: 734 234 12 454 1181 1384 268 1917 Fragments arranged by size: 1917 1384 1181 734 454 268 234 12 AflII C'TTAA_G Cuts at: 820 1017 3199 820 Size: 197 2182 3805 Fragments arranged by size: 3805 2182 197 AflIII A'CryG_T Cuts at: 1203 2319 5083 6060 1203 Size: 1116 2764 977 1327 Fragments arranged by size: 2764 1327 1116 977 AgeI A'CCGG_T Cuts at: 5015 5015 Size: 6184 AhdI GACnn_n'nnGTC Cuts at: 4913 4913 Size: 6184 AleI CACnn'nnGTG Cuts at: 1166 3299 1166 Size: 2133 4051 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6184 AlwNI CAG_nnn'CTG Cuts at: 3927 5499 3927 Size: 1572 4612 ApaLI G'TGCA_C Cuts at: 4241 5397 4241 Size: 1156 5028 ApoI r'AATT_y Cuts at: 1493 2560 3095 3493 3719 3871 5980 1493 Size: 1067 535 398 226 152 2109 1697 Fragments arranged by size: 2109 1697 1067 535 398 226 152 AseI AT'TA_AT Cuts at: 160 1032 4738 160 Size: 872 3706 1606 Fragments arranged by size: 3706 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 AvaI C'yCGr_G Cuts at: 1870 1943 3329 3405 3509 1870 Size: 73 1386 76 104 4545 Fragments arranged by size: 4545 1386 104 76 73 BamHI G'GATC_C Cuts at: 3514 3881 3514 Size: 367 5817 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2066 3505 4960 618 Size: 325 217 627 279 1439 1455 1842 Fragments arranged by size: 1842 1455 1439 627 325 279 217 BanII G_rGCy'C Cuts at: 1391 3503 1391 Size: 2112 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2323 2854 928 Size: 971 424 531 4258 Fragments arranged by size: 4258 971 531 424 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4375 4409 1903 Size: 34 50 34 2354 34 3678 Fragments arranged by size: 3678 2354 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4076 5286 4076 Size: 1210 4974 BclI T'GATC_A Cuts at: 1548 2383 3291 1548 Size: 835 908 4441 Fragments arranged by size: 4441 908 835 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4795 136 Size: 107 122 71 631 605 337 2786 1525 Fragments arranged by size: 2786 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2601 3045 6179 2601 Size: 444 3134 2606 Fragments arranged by size: 3134 2606 444 BlpI GC'TnA_GC Cuts at: 2909 3601 2909 Size: 692 5492 Bme1580I G_kGCm'C Cuts at: 1790 4245 5401 1790 Size: 2455 1156 2573 Fragments arranged by size: 2573 2455 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6184 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3915 4873 442 Size: 416 53 83 399 24 2498 958 1753 Fragments arranged by size: 2498 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6184 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2425 3274 2425 Size: 849 5335 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2408 2759 2963 3455 4844 1239 Size: 1169 351 204 492 1389 2579 Fragments arranged by size: 2579 1389 1169 492 351 204 Bpu10I CC'TnA_GC Cuts at: 3447 3447 Size: 6184 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3017 3499 4309 5174 5472 3017 Size: 482 810 865 298 3729 Fragments arranged by size: 3729 865 810 482 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2939 4847 882 Size: 617 1440 1908 2219 Fragments arranged by size: 2219 1908 1440 617 BsaAI yAC'GTr Cuts at: 493 1204 3975 493 Size: 711 2771 2702 Fragments arranged by size: 2771 2702 711 BsaBI GATnn'nnATC Cuts at: 6178 6178 Size: 6184 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3991 4373 5008 275 Size: 53 83 186 936 2458 382 635 1451 Fragments arranged by size: 2458 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4617 5015 5289 5436 5847 4617 Size: 398 274 147 411 4954 Fragments arranged by size: 4954 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3057 3087 1178 Size: 30 165 30 1654 30 4275 Fragments arranged by size: 4275 1654 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6184 BseYI C'CCAG_C Cuts at: 2038 2374 2818 3002 5387 5883 2038 Size: 336 444 184 2385 496 2339 Fragments arranged by size: 2385 2339 496 444 336 184 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5611 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3634 4395 5423 1540 Size: 275 186 1633 761 1028 2301 Fragments arranged by size: 2301 1633 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3453 4245 4330 5401 3453 Size: 792 85 1071 4236 Fragments arranged by size: 4236 1071 792 85 BsmI GAATG_Cn' Cuts at: 3695 3788 3695 Size: 93 6091 BsmBI CGTCTCn'nnnn_ Cuts at: 2916 2916 Size: 6184 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3453 3503 4245 4330 5401 1391 Size: 399 1663 50 742 85 1071 2174 Fragments arranged by size: 2174 1663 1071 742 399 85 50 BspEI T'CCGG_A Cuts at: 5847 5847 Size: 6184 BspHI T'CATG_A Cuts at: 3351 4073 5803 3351 Size: 722 1730 3732 Fragments arranged by size: 3732 1730 722 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3462 3539 844 Size: 2618 77 3489 Fragments arranged by size: 3489 2618 77 BsrBI CCG'CTC Cuts at: 1394 1823 4071 1394 Size: 429 2248 3507 Fragments arranged by size: 3507 2248 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2050 4679 4853 65 Size: 1183 802 2629 174 1396 Fragments arranged by size: 2629 1396 1183 802 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4828 5015 1700 Size: 110 3018 187 2869 Fragments arranged by size: 3018 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6184 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6184 BssSI C'ACGA_G Cuts at: 2075 2129 4244 5256 2075 Size: 54 2115 1012 3003 Fragments arranged by size: 3003 2115 1012 54 BstAPI GCAn_nnn'nTGC Cuts at: 794 2091 794 Size: 1297 4887 BstBI TT'CG_AA Cuts at: 4988 4988 Size: 6184 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6184 BstXI CCAn_nnnn'nTGG Cuts at: 2818 2818 Size: 6184 BstYI r'GATC_y Cuts at: 1972 2601 3045 3514 3881 4266 4283 5735 5821 Size: 629 444 469 367 385 17 1452 86 Cuts at: 5821 5833 6179 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 629 469 444 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3312 3312 Size: 6184 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5632 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2245 487 Size: 683 56 1019 4426 Fragments arranged by size: 4426 1019 683 56 BtsI GCAGTG_nn' Cuts at: 2361 2813 2943 3025 3700 4494 4514 2361 Size: 452 130 82 675 794 20 4031 Fragments arranged by size: 4031 794 675 452 130 82 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6184 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6149 DraI TTT'AAA Cuts at: 3488 3488 Size: 6184 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5191 809 Size: 507 3875 1802 Fragments arranged by size: 3875 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2212 2637 2874 Size: 54 1425 50 275 18 382 425 237 Cuts at: 2874 2896 3631 4520 6149 8 Size: 22 735 889 1629 43 Fragments arranged by size: 1629 1425 889 735 425 382 275 237 54 50 43 22 18 EagI C'GGCC_G Cuts at: 1537 1812 3631 1537 Size: 275 1819 4090 Fragments arranged by size: 4090 1819 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3141 3200 3380 4114 1353 Size: 1788 59 180 734 3423 Fragments arranged by size: 3423 1788 734 180 59 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4771 5145 5291 387 Size: 1064 138 3182 374 146 1280 Fragments arranged by size: 3182 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2366 2408 2600 2612 2759 2963 3066 Size: 393 734 42 192 12 147 204 103 Cuts at: 3066 3455 4247 4844 5631 5899 1239 Size: 389 792 597 787 268 1524 Fragments arranged by size: 1524 792 787 734 597 393 389 268 204 192 147 103 42 12 EcoO109I rG'GnC_Cy Cuts at: 2340 2785 2340 Size: 445 5739 EcoRI G'AATT_C Cuts at: 3493 3871 3493 Size: 378 5806 EcoRV GAT'ATC Cuts at: 2182 3154 2182 Size: 972 5212 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3214 3214 Size: 6184 FspI TGC'GCA Cuts at: 4690 4690 Size: 6184 HaeII r_GCGC'y Cuts at: 1678 5331 1678 Size: 3653 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2698 2730 3057 3089 3305 Size: 32 135 32 1126 32 327 32 216 Cuts at: 3305 3337 3388 3420 4825 4857 4899 4931 1373 Size: 32 51 32 1405 32 42 32 2626 Fragments arranged by size: 2626 1405 1126 327 216 135 51 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3528 3774 669 Size: 988 281 1590 246 3079 Fragments arranged by size: 3079 1590 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6184 HpaI GTT'AAC Cuts at: 3774 3774 Size: 6184 KpnI G_GTAC'C Cuts at: 3509 3509 Size: 6184 MfeI C'AATT_G Cuts at: 3783 3783 Size: 6184 MluI A'CGCG_T Cuts at: 6060 6060 Size: 6184 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3264 5298 5482 1305 Size: 1959 2034 184 2007 Fragments arranged by size: 2034 2007 1959 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2214 2876 10 Size: 54 1425 343 382 662 3318 Fragments arranged by size: 3318 1425 662 382 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2690 3299 4142 4501 4660 518 Size: 648 1524 609 843 359 159 2042 Fragments arranged by size: 2042 1524 843 648 609 359 159 MspA1I CmG'CkG Cuts at: 1606 2440 2551 3299 3600 4277 5425 5670 1606 Size: 834 111 748 301 677 1148 245 2120 Fragments arranged by size: 2120 1148 834 748 677 301 245 111 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5632 NdeI CA'TA_TG Cuts at: 387 387 Size: 6184 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6184 NotI GC'GGCC_GC Cuts at: 3631 3631 Size: 6184 NspI r_CATG'y Cuts at: 1132 1229 2836 3542 5087 1132 Size: 97 1607 706 1545 2229 Fragments arranged by size: 2229 1607 1545 706 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6184 PciI A'CATG_T Cuts at: 5083 5083 Size: 6184 PflMI CCAn_nnn'nTGG Cuts at: 1108 2367 2872 1108 Size: 1259 505 4420 Fragments arranged by size: 4420 1259 505 PfoI T'CCnGG_A Cuts at: 2889 2889 Size: 6184 PmeI GTTT'AAAC Cuts at: 3488 3488 Size: 6184 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4253 4285 5766 5798 4253 Size: 32 1481 32 4639 Fragments arranged by size: 4639 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2340 2785 2340 Size: 445 5739 PshAI GACnn'nnGTC Cuts at: 1613 3164 1613 Size: 1551 4633 PsiI TTA'TAA Cuts at: 3754 3754 Size: 6184 PspXI vC'TCGA_Gb Cuts at: 3329 3329 Size: 6184 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2831 2863 2831 Size: 32 6152 PstI C_TGCA'G Cuts at: 830 3285 3429 3536 830 Size: 2455 144 107 3478 Fragments arranged by size: 3478 2455 144 107 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6184 PvuII CAG'CTG Cuts at: 2440 2551 2440 Size: 111 6073 RsrII CG'GwC_CG Cuts at: 2466 2466 Size: 6184 SalI G'TCGA_C Cuts at: 1936 3526 1936 Size: 1590 4594 SanDI GG'GwC_CC Cuts at: 2340 2340 Size: 6184 SapI GCTCTTCn'nnn_ Cuts at: 3200 3200 Size: 6184 SbfI CC_TGCA'GG Cuts at: 3536 3536 Size: 6184 ScaI AGT'ACT Cuts at: 4432 6144 4432 Size: 1712 4472 SfcI C'TryA_G Cuts at: 826 1045 3129 3281 3425 3532 4667 5348 5539 Size: 219 2084 152 144 107 1135 681 191 Cuts at: 5539 826 Size: 1471 Fragments arranged by size: 2084 1471 1135 681 219 191 152 144 107 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6184 SmaI CCC'GGG Cuts at: 1872 3511 1872 Size: 1639 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3032 3199 3329 3405 3478 4288 Size: 197 926 1089 167 130 76 73 810 Cuts at: 4288 5189 5451 820 Size: 901 262 1553 Fragments arranged by size: 1553 1089 926 901 810 262 197 167 130 76 73 SnaBI TAC'GTA Cuts at: 493 3975 493 Size: 3482 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6184 SphI G_CATG'C Cuts at: 1132 2836 3542 1132 Size: 1704 706 3774 Fragments arranged by size: 3774 1704 706 SspI AAT'ATT Cuts at: 5 52 4108 5 Size: 47 4056 2081 Fragments arranged by size: 4056 2081 47 StuI AGG'CCT Cuts at: 1517 3173 1517 Size: 1656 4528 StyI C'CwwG_G Cuts at: 513 1065 2139 2209 2518 2877 3623 513 Size: 552 1074 70 309 359 746 3074 Fragments arranged by size: 3074 1074 746 552 359 309 70 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2483 2526 4211 4228 4381 4566 5011 Size: 1255 903 43 1685 17 153 185 445 Cuts at: 5011 325 Size: 1498 Fragments arranged by size: 1685 1498 1255 903 445 185 153 43 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2838 4430 6142 Size: 274 80 33 51 1443 861 1592 1712 Cuts at: 6142 96 Size: 138 Fragments arranged by size: 1712 1592 1443 861 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6144 6176 6144 Size: 32 6152 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6184 XbaI T'CTAG_A Cuts at: 3520 3520 Size: 6184 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2222 2272 2222 Size: 50 6134 XhoI C'TCGA_G Cuts at: 1943 3329 3405 1943 Size: 1386 76 4722 Fragments arranged by size: 4722 1386 76 XmaI C'CCGG_G Cuts at: 1870 3509 1870 Size: 1639 4545 XmnI GAAnn'nnTTC Cuts at: 4313 4313 Size: 6184 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3992 276 Size: 53 83 186 936 2458 2468 Fragments arranged by size: 2468 2458 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI SacI SacII SexAI SfiI SfoI SrfI SwaI