(Linear) (Six-base) MAPSORT of: KIEE4472.seq Check: 3064 from: 1 to: 1479 KIEE4472.orf With 182 enzymes: SgfI * March 22, 2012 18:08 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1457 1479 Size: 1457 22 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 361 595 607 1061 1479 Size: 361 234 12 454 418 Fragments arranged by size: 454 418 361 234 12 AflII C'TTAA_G Cuts at: 0 1194 1479 Size: 1194 285 AflIII A'CryG_T Cuts at: 0 314 1479 Size: 314 1165 AleI CACnn'nnGTG Cuts at: 0 1294 1479 Size: 1294 185 ApoI r'AATT_y Cuts at: 0 555 1090 1479 Size: 555 535 389 Fragments arranged by size: 555 535 389 AvaI C'yCGr_G Cuts at: 0 1324 1400 1479 Size: 1324 76 79 Fragments arranged by size: 1324 79 76 BanI G'GyrC_C Cuts at: 0 61 1479 Size: 61 1418 BbsI GAAGACnn'nnnn_ Cuts at: 0 318 849 1479 Size: 318 531 630 Fragments arranged by size: 630 531 318 BclI T'GATC_A Cuts at: 0 378 1286 1479 Size: 378 908 193 Fragments arranged by size: 908 378 193 BglII A'GATC_T Cuts at: 0 596 1040 1479 Size: 596 444 439 Fragments arranged by size: 596 444 439 BlpI GC'TnA_GC Cuts at: 0 904 1479 Size: 904 575 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 420 1269 1479 Size: 420 849 210 Fragments arranged by size: 849 420 210 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 403 754 958 1450 1479 Size: 403 351 204 492 29 Fragments arranged by size: 492 403 351 204 29 Bpu10I CC'TnA_GC Cuts at: 0 1442 1479 Size: 1442 37 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1012 1479 Size: 1012 467 BsaI GGTCTCn'nnnn_ Cuts at: 0 934 1479 Size: 934 545 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1052 1082 1479 Size: 1052 30 397 Fragments arranged by size: 1052 397 30 BseYI C'CCAG_C Cuts at: 0 33 369 813 997 1479 Size: 33 336 444 184 482 Fragments arranged by size: 482 444 336 184 33 BsiHKAI G_wGCw'C Cuts at: 0 1448 1479 Size: 1448 31 BsmBI CGTCTCn'nnnn_ Cuts at: 0 911 1479 Size: 911 568 Bsp1286I G_dGCh'C Cuts at: 0 1448 1479 Size: 1448 31 BspHI T'CATG_A Cuts at: 0 1346 1479 Size: 1346 133 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1457 1479 Size: 1457 22 BsrDI GCAATG_nn' Cuts at: 0 45 1479 Size: 45 1434 BssSI C'ACGA_G Cuts at: 0 70 124 1479 Size: 70 54 1355 Fragments arranged by size: 1355 70 54 BstAPI GCAn_nnn'nTGC Cuts at: 0 86 1479 Size: 86 1393 BstXI CCAn_nnnn'nTGG Cuts at: 0 813 1479 Size: 813 666 BstYI r'GATC_y Cuts at: 0 596 1040 1479 Size: 596 444 439 Fragments arranged by size: 596 444 439 Bsu36I CC'TnA_GG Cuts at: 0 1307 1479 Size: 1307 172 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 240 1479 Size: 240 1239 BtsI GCAGTG_nn' Cuts at: 0 356 808 938 1020 1479 Size: 356 452 130 82 459 Fragments arranged by size: 459 452 356 130 82 EaeI y'GGCC_r Cuts at: 0 207 632 869 891 1479 Size: 207 425 237 22 588 Fragments arranged by size: 588 425 237 207 22 EarI CTCTTCn'nnn_ Cuts at: 0 1136 1195 1375 1479 Size: 1136 59 180 104 Fragments arranged by size: 1136 180 104 59 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 361 403 595 607 754 958 1061 1450 Size: 361 42 192 12 147 204 103 389 Cuts at: 1450 1479 Size: 29 Fragments arranged by size: 389 361 204 192 147 103 42 29 12 EcoO109I rG'GnC_Cy Cuts at: 0 335 780 1479 Size: 335 445 699 Fragments arranged by size: 699 445 335 EcoRV GAT'ATC Cuts at: 0 177 1149 1479 Size: 177 972 330 Fragments arranged by size: 972 330 177 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1209 1479 Size: 1209 270 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 693 725 1052 1084 1300 1332 1383 1415 Size: 693 32 327 32 216 32 51 32 Cuts at: 1415 1479 Size: 64 Fragments arranged by size: 693 327 216 64 51 32 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1259 1479 Size: 1259 220 MscI TGG'CCA Cuts at: 0 209 871 1479 Size: 209 662 608 Fragments arranged by size: 662 608 209 MslI CAynn'nnrTG Cuts at: 0 685 1294 1479 Size: 685 609 185 Fragments arranged by size: 685 609 185 MspA1I CmG'CkG Cuts at: 0 435 546 1294 1479 Size: 435 111 748 185 Fragments arranged by size: 748 435 185 111 NspI r_CATG'y Cuts at: 0 831 1479 Size: 831 648 PflMI CCAn_nnn'nTGG Cuts at: 0 362 867 1479 Size: 362 505 612 Fragments arranged by size: 612 505 362 PfoI T'CCnGG_A Cuts at: 0 884 1479 Size: 884 595 PpuMI rG'GwC_Cy Cuts at: 0 335 780 1479 Size: 335 445 699 Fragments arranged by size: 699 445 335 PshAI GACnn'nnGTC Cuts at: 0 1159 1479 Size: 1159 320 PspXI vC'TCGA_Gb Cuts at: 0 1324 1479 Size: 1324 155 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 826 858 1479 Size: 826 32 621 Fragments arranged by size: 826 621 32 PstI C_TGCA'G Cuts at: 0 1280 1424 1479 Size: 1280 144 55 Fragments arranged by size: 1280 144 55 PvuII CAG'CTG Cuts at: 0 435 546 1479 Size: 435 111 933 Fragments arranged by size: 933 435 111 RsrII CG'GwC_CG Cuts at: 0 461 1479 Size: 461 1018 SanDI GG'GwC_CC Cuts at: 0 335 1479 Size: 335 1144 SapI GCTCTTCn'nnn_ Cuts at: 0 1195 1479 Size: 1195 284 SfcI C'TryA_G Cuts at: 0 1124 1276 1420 1479 Size: 1124 152 144 59 Fragments arranged by size: 1124 152 144 59 SmlI C'TyrA_G Cuts at: 0 1027 1194 1324 1400 1473 1479 Size: 1027 167 130 76 73 6 Fragments arranged by size: 1027 167 130 76 73 6 SphI G_CATG'C Cuts at: 0 831 1479 Size: 831 648 StuI AGG'CCT Cuts at: 0 1168 1479 Size: 1168 311 StyI C'CwwG_G Cuts at: 0 134 204 513 872 1479 Size: 134 70 309 359 607 Fragments arranged by size: 607 359 309 134 70 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 478 521 1479 Size: 478 43 958 Fragments arranged by size: 958 478 43 TatI w'GTAC_w Cuts at: 0 833 1479 Size: 833 646 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 217 267 1479 Size: 217 50 1212 Fragments arranged by size: 1212 217 50 XhoI C'TCGA_G Cuts at: 0 1324 1400 1479 Size: 1324 76 79 Fragments arranged by size: 1324 79 76 Enzymes that do cut: AarI AcuI AflII AflIII AleI ApoI AvaI BanI BbsI BclI BglII BlpI BplI BpmI Bpu10I BpuEI BsaI BsaXI BseYI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BssSI BstAPI BstXI BstYI Bsu36I BtgZI BtsI EaeI EarI Eco57MI EcoO109I EcoRV FalI Hin4I MmeI MscI MslI MspA1I NspI PflMI PfoI PpuMI PshAI PspXI PsrI PstI PvuII RsrII SanDI SapI SfcI SmlI SphI StuI StyI TaqII TatI XcmI XhoI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanII BbeI BbvCI BcgI BciVI BfrBI BglI Bme1580I BmgBI BmrI BmtI BsaAI BsaBI BsaHI BsaWI BseRI BsgI BsiEI BsiWI BsmI BspEI BsrBI BsrFI BsrGI BssHII BstBI BstEII BstZ17I BtgI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PsiI PspOMI PvuI SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TstI Tth111I XbaI XmaI XmnI ZraI